观察百合提取物(Lily extracted substances,LES)、知母提取物(Zhimu extracted substances,ZES)、百合知母汤提取物(Lily-Zhimu extracted substances,L-ZES)对慢性不可预见性温和刺激(Chronic unpredictable mild stress,CUMS)配合孤养所致大鼠抑郁模型的影响。方法:60只雄性SD大鼠随机分为正常组、模型组、百合提取物组、知母提取物组、百合知母汤提取物组、阳性药氟西汀组。采用CUMS配合孤养诱发抑郁模型。连续灌胃给药6周,观察大鼠体重变化,运用旷场实验、糖水偏爱实验评定大鼠的行为学特征。以酶联免疫吸附法(ELISA)检测血清多巴胺(DA)、促肾上腺皮质激素(ACTH)的含量。结果:与正常组相比,模型组大鼠的体重增加缓慢、行为学特征下降明显,血清DA含量下降、ACTH含量升高,差异有统计学意义(P0.01),说明CUMS配合孤养可致大鼠出现抑郁行为。与模型组比较,百合提取物组、知母提取物组及百合知母汤提取物组、阳性药氟西汀(FHP)组能显著对抗抑郁刺激,大鼠的体重增加、行为学特征改善,DA的含量提高ACTH的含量降低。结论:百合提取物、知母提取物、百合知母汤提取物有很好的抗抑郁作用,其机制可能与调节抑郁症模型大鼠体内神经递质紊乱及调节HPA轴的功能、抑制大鼠HPA轴亢进有关。

Objective:To observe the antidepressant effect of LES;ZES;L-ZES on the model of the depressive rats that are induced by the CUMS and separate feeding method. Methods 60 male SD rats were randomLy divided into normal group, model group, LES group, ZES group, L-ZES group, positive drug fluoxetine (FHP)group. Use CUMS and separate feeding induced model of the depressive rats, All the rats are continuously gavaged for 6 weeks , and their data of changing weight were taken.And the Open Field Test and the Preference for Sucrose Test were used to assessed behavioral characteristics of the rats. The content of serum DA and ACTH were detected by using ELISA.Results Compared with the normal group,the model group gained weight slower and their behavioral characteristics decreased significantly. The DA content in serum decreased and the ACTH increased .The difference was statistically significant (P<0.01).It indicates that CUMS and isolated raising can induce depression phenomenon in rats .When it

探讨柴胡加龙骨牡蛎汤对慢性应激抑郁大鼠海马BDNF表达的影响。方法:选择SD雄性大鼠50只,随机分为对照组、抑郁模型组(模型组)、柴胡加龙骨牡蛎汤低剂量组、柴胡加龙骨牡蛎高剂量组、氟西汀组5组,每组10只。用慢性应激结合孤养方法造模,免疫组化法观察大鼠海马BDNF的表达。结果:抑郁模型大鼠海马BDNF表达降低(P0.05),柴胡加龙骨牡蛎汤可不同程度减轻上述变化。中药高剂量组与氟西汀组作用相近(P0.05)。结论:柴胡加龙骨牡蛎汤可增加抑郁模型大鼠海马的BDNF表达,这可能是其抗抑郁作用的环节之一。

Objective:To discuss the effects of radix bupleuri ,fossil fragments and oyster soup on the expression in chronic mild stress depression rats ’ hippocampus .Method:Randomly divide 50 SD male rats into five groups , 10 SD rats in each group, which were control group, depression model group(model group), low and high dose groups of radix bupleuri, fossil fragments and oyster soup , fluoxetine group .Chronic mild stress with solitary condition and immunohistochemistry was taken to establish model to observe the expression of the hippocampus BDNF in rats .Results:Expression of hippo-campus BDNF in depression model group was lower (P 0 .05 ) . Conclusion:Radix bupleuri ,fossil fragments and oyster soup can increase the expression of hippocampus BDNF in de -pression models .This may be one phrase in antidepression .

目的：研究对慢性应激抑郁模型大鼠海马BDNF表达与认知功能损伤之间的关系。方法采用21d慢性不可预见的温和应激处理结合孤养制备抑郁大鼠模型。运用被动躲避实验评定大鼠的学习记忆能力，采用荧光定量PCR技术和免疫组织化学技术检测BDNF基因和蛋白的表达变化。结果抑郁组大鼠与正常对照组比较，体重、糖水消耗量、旷场试验运动均减少，被动规避缺陷，以上行为学改变差异均有统计学意义（P<0.05）。免疫组织化学和实时荧光定量PCR技术检测均显示：抑郁组大鼠海马各区BDNF蛋白和mRNA相对表达量均显著低于正常对照组，差异有统计学意义（P<0.01）。结论慢性应激抑郁大鼠海马区BDNF表达下降可使认知障碍加重。

Objectives To make clear of the relationship between brain-derived neurotrophic factor (BDNF) expression and cognitive impairment in depression rats. Methods Isolated-living condition and chronic unpredictable mild stress are applied successively to set up depression rat model . Learning and memory function is evaluated by passive avoidance test.Real Time-PCR and immunohistochemistry method are applied to detect the BDNF mRNA and BDNF protein. Results Compared with the normal control group,the depression group have significant changes (P<0.05):weight loss,the decrease of sucrose solution consumption,the drop of open-field behavior, the deficiency of passive avoid test. Compared with the normal control group,BDNF expression is significantly decreased in the hippocampus of the depression group (P<0.01). Conclusions The decreased expression of BDNF in the hippocampus of depression rats may make the cognitive impairment increase.

观察坤宁安对去卵巢抑郁模型大鼠海马Bcl-2和Bax表达的影响。方法:去卵巢雌性SD大鼠50只,随机分为5组:去卵巢组(对照组)、去卵巢抑郁模型组(模型组)、去卵巢坤宁安低剂量组(坤宁安低剂量组)、去卵巢坤宁安高剂量组(坤宁安高剂量组)、去卵巢氟西汀组(氟西汀组),采用孤养及慢性应激方式造模,观测大鼠海马Bcl-2和Bax表达的变化。结果:抑郁模型大鼠的Bax阳性细胞表达增强,Bcl-2阳性细胞表达降低,坤宁安可使模型大鼠海马Bcl-2表达上调,降低Bax的表达。结论:坤宁安能够影响抑郁模型大鼠海马Bcl-2和Bax的表达,可能是其抗抑郁作用机理之一。

Objective:This thesis is aimed at observing the effect of Kunningan on the expression of hippocampus Bcl -2 and Bax of ovariectomy depressive rats .Methods:Divide ovariectomy female SD rats into five groups randomly : ovariec-tomy group(control group), ovariectomy depression group(model group), ovariectomy Kunningan low dose group (low dose group), ovariectomy Kunningan high dose group ( high dose group ), ovariectomy Fluoxetine group ( Fluoxetine group).The methods of solitary condition and chronic stress were taken to establish the models , then with the methods to observe the changes on the expression of rats ’ hippocampus Bcl -2 and Bax.Results:The expression of rats’ Bax positive cells in depression group was enhanced and Bcl -2 was decreased.Kunningan could change the expressions of Bcl -2 and Bax.Conclusion:Kunningan can influence the expressions of hippocampus Bcl -2 and Bax in depressive model rats.That may be one of the mechanisms to treat the depression .

探讨β-细辛醚对抑郁模型大鼠海马组织及Bax、Bcl-2蛋白表达的影响。方法:经过敞箱实验选取得分相近的2~3个月龄SD大鼠80只,按随机数字表法分为四组,每组20只,结合孤养并给予慢性轻度不可预见刺激,复制抑郁模型,于造模成功第2天开始,分别给予氟西汀组和β-细辛醚组1.2 mg/(kg·d)氟西汀和25 mg/(kg·d)β-细辛醚灌胃,模型组与正常组给予等体积生理盐水。于第21天对大鼠处死取脑,免疫组织化学法检测Bax、Bcl-2蛋白相对含量。结果:模型组大鼠海马组织神经元数目减少,散在排列,细胞核发生固缩变形,CA1区,CA3区变化显著。与模型组比较,β-细辛醚组、氟西汀组Bax蛋白表达均显著降低(P0.01)。结论:β-细辛醚可以降低大鼠海马组织Bax蛋白表达,促进Bcl-2蛋白表达。

To investigate the effect ofβ-assrone on the hippocampus and the expressions of protein Bax and Bcl-2 in the hippocampus of depression model rats.Method:According to the open field test,adult Sprague-Dawley rates with similar scores were randomly divided into 4 groups,each group had 20 cases. Solitary and chronic mild unpredictability stimulation on each one except the normal control group,copy the model of depression. On the second day of a successful model replication,the fluoxetine control group and theβ-asarone group were given 1.2 mg/(kg·d)the fluoxetine and 25 mg/(kg·d)theβ-asarone. The model control group and the normal control group were given equal volumes of normal saline. On the 21th day,rats were to be put to death and take brain tissue. The protein expression of Bax and Bcl-2 in the hippocampus were tested by immunohistochemical staining method.Result:The numbers of neurons were reduced in the hippocampus of model group,scattered in the arrangement,the nucle