采用高效液相色谱蒸发光散射法(HPLC-ELSD)测定珠子参根茎中新化合物24(R)-珠子参苷 R1的质量浓度.色谱条件为:Aglilent Zorbax SB-C18(ODS,4.6 mm ×250 mm,5μm)色谱柱,流动相V(甲醇)∶V(水)=58∶42,流速1.0 mL/min,柱温25℃,载气压力1.94×105 Pa,漂移管温度85℃,撞击器关闭,进样量20μL.结果表明:在0.40-20.0μg内,24(R)-珠子参苷R1线性关系较好,r=0.9993(n=6);24(R)-珠子参苷 R1的平均回收率为98.7%,RSD=1.28%(n=6).
The determination of the new compound of 24(R)-majoroside R1 in Panaxjaponicus C.A. Mey.var.Major (Burk.)C.Y.Wu et K.M.Feng was developed by HPLC-ELSD method.The conditions of chromatography were Aglient Zorbax SB-C18 Column (ODS,4.6 mm×250 mm,5μm) was adopted;mobile phase was composed ofV(methanol)∶V(water)=58∶42;flow rate was set at 1.0 mL/min;temperature of column was set at 25 ℃;carrier gas pressure was set at 1.94×105 Pa tube temperature of ELSD was set at 85 ℃;impactor was set in closed state.As the results show in the range of 0.4-20.0μg,the linearity of 24(R)-majoroside R1 is good,r=0.999 3(n=6),the average recovery was 98.7%,and RSD=1.28% (n=6).