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双语推荐:表达

日本の文芸では1900年代から「旅愁「」旅情」が流行語になり、1910年代にかけて郷土色の強い情緒や情調を醸し出すことを狙う表現が開花した。これまであまり論じられてこなかった1910年代の人気作家、長田幹彦による祇園情緒の表現に焦点を当て、その特徴を述べ、そして、その情緒の表現が1920年代から1930年代にかけて「、乾いた抒情」に転換してゆくことを三好達治の詩を例にとって示す。背景には、印象主義から耽美主義、象徴主義、感情移入美学の流行、そして狭義のモダニズムへと芸術概念の大きな変容があるが、ここでは、それに詳しく立ち入らない。
The words“ryoshū”and“ryojō”, meaning the melancholy during a journey, became popular from the 1900s, and gained buzzword status in the 1910’s when expressions of emotion filled with local color burst into usage. This paper focuses on the representation of Gion by Mikihiko Nagata in the 1910s, and introduces its features. It then takes Tatsuji Miyoshi’s poems as an example to show the emotional changes occurring from the 1920s to the 1930s.

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转型期中国由于利益表达制度尚不够健全,公民利益表达需求的多样性与政府制度供给不足存在尖锐矛盾,尤其是社会弱势群体长期利益表达不畅,导致群体性事件频发。弱势群体利益表达问题的关键是解决其利益表达的实效性问题。当前弱势群体利益表达的无效或低效背后,既有表达渠道和表达制度供给的滞后性因素,也同弱势群体自身的局限性及党政官员的能力和素质缺陷密不可分。要想提升弱势群体利益表达有效性从而消减群体性事件,单凭完善弱势群体利益表达制度本身是不够的,还须有其他的措施来加以保障。这其中,培育成熟的表达主体是有效表达的前提保障,完善配套制度是有效表达的根本保障,党政官员的责任心和能力建设是有效表达的基本保障。
During the transition period in China, as the interests expression system is still not perfect, sharp contradictions between diversity of citizen’ s interests expression demand and deficiency of govern-ment’s institution supply, especially the interests expression of social vulnerable groups is not smooth, which have caused frequent mass incidents.The key problem of vulnerable groups’ interests expression is expression effectiveness.Currently, the reasons of ineffective or inefficient interests expression of vulnerable groups includes both expression channels and expression system factors.Also, limitations of vulnerable groups and ability and quality defects of expression object are important factors.In order to improve the interests expression effectiveness of vulnerable groups and then reduce mass incidents, only by perfecting the interests expression system is not enough, other measures must be taken, too.Among them, cultivating mature expression subject is the premise

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目的:构建miR101的慢病毒表达系统并验证其对牙周膜细胞PLAP-1基因的表达调控。方法:用矿化培养液培养牙周膜细胞,使用pLVX-shRNA1慢病毒表达系统构建获得超表达miR101和抑制表达miR101的慢病毒;用qPCR验证miR101的表达水平,使用Western检测PLAP-1的表达水平。结果:成功获得了miR101的慢病毒表达系统,用于转染矿化诱导液培养的人牙周膜细胞。过表达miR101可抑制PLAP-1的表达,抑制表达miR101可增强PLAP-1的表达。结论:miR101可以调节PLAP-1基因并参与人牙周膜细胞骨向分化中PLAP-1基因表达的调节, miR101可能在人牙周膜细胞骨向分化中发挥调节作用。
AIM:To investigate whether miR101 regulates the gene expression of PLAP-1 in human perio-dontal ligament cells ( PDLCs) .METHODS:Overexpression and downexpression systems of miR101 were constructed with pLVX-shRNA1 system.PDLCs were cultured with osteogenesis induction medium and infected by the miR101 overexpression and downexpression systems respectively.Western blot was performed to confirm the effects of these constructs on PLAP-1 gene expression.RESULTS:Constructs of lentiviral-miR101 were obtained.Overexpression of miR101 reduced the expression level of PLAP-1.Downexpression of miR101 increased the expression level of PLAP-1.CONCLUSION:miR101 regulates PLAP-1 expression during osteogenic differentiation of PDLCs, sugges-ting that miR101 may play important roles in the osteogenic differentiation of PDLCs.

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目的观察乳癌基因1(BRCA1)在鼻咽癌中的表达水平并分析其表达水平与预后的关系。方法采用免疫组化S-P法检测100例患者鼻咽癌组织中BRCA1蛋白的表达水平,阳性细胞计数≥50%定为BRCA1高表达组;50%及不表达者定为BRCA1低表达组。应用χ2检验对BRCA1表达水平与临床资料进行关联性分析。结果 100例患者中13例失访,BRCA1高表达者37例(42.5%),低表达者50例(57.5%);BRCA1表达与患者的临床分期(P=0.383)、原发灶退缩情况(P=0.434)、肿瘤发生远处转移(P=0.055)无明显关联性,高表达组生存率稍高于低表达组,但差异无统计学意义(P0.05)。结论鼻咽癌组织中BRCA1蛋白的表达与临床分期和原发灶退缩程度无关,但BRCA1低表达者更易出现远处转移的趋势。
Objective To investigate the protein expression of Breast cancer 1(BRCA1) in nasopharyngeal carcinoma(NPC) and to analyze the relationship of BRCA1 expression with the prognosis.Methods The expression of BRCA1 proteins in the tissue of nasopharyngeal carcinoma in 100 patients with NPC was detected by immunohisto-chemistry.The high BRCA1 expression group included patients whose biopsy specimens showed at least 50%immu-nopositive tumor cells;The low BRCA1 expression group included patients whose biopsy specimens showed less than 50% immunopositive tumor cells , including negative expression .The correlations between protein expression and clin-ical data were assessed using the chi-square test.Results Thirteen patients failed to be followed-up.And in the rest 87 patients, the high BRCA1 expression group and low BRCA 1 expression group included 37 patients(42.5%) and 50 patients(57.5%) respectively.No significant differences were found between the expression of BRCA 1 proteins and TNM stag

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目的检测乳腺癌组织中Bmi1蛋白的表达,探讨Bmi1蛋白的表达与乳腺癌临床病理特征相关性。方法收集79例乳腺癌组织标本,利用免疫组化的方法检测Bmi1和E-cadherin的表达,分析Bmi1蛋白的表达与乳腺癌临床病理特征和E-cadherin的表达相关性。结果 Bmi1蛋白在乳腺癌中的表达率约31.6%(25/79),Bmi1蛋白高表达与淋巴结的转移呈正相关;Bmi1蛋白的表达与年龄、肿瘤大小、组织学分级无关。E-cadherin的表达率为57.0%(45/79),Bmi1蛋白的表达与E-cadherin的表达呈负相关。结论乳腺癌组织中Bmi1蛋白的表达与乳腺癌的转移有关,有可能成为预测乳腺癌发生上皮间质转化的新分子标记物。
Objective To detect the expression of Bmi1 in breast cancer and analyze the relationship between its expression and breast cancer clinicopathological features. Methods Samples of 79 breast cancer tissues from different patients were collected. Immunohistochemistry was performed to detect the expression of Bmi1 and E - cadherin. The relationship between Bmi1 expression and the clinicopathological features and E - cadherin expression of breast cancers were analyzed. Results Bmi1 was expressed in about 31. 6%(25/79)of breast cancers tissues. The expression of Bmi1 was positively correlated with lymph node metastasis,but not related to age,tumor size and histologic grade. The expression of E - cadherin was about 57. 0%(45/79),and negatively correlated with Bmi1 expression. Conclusion Bmi1expression was related to metastasis of breast cancer. The Bmil expression may be a new molecular marker of epithelial - mesenchymal transition in breast cancer.

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目的:探讨在乳腺癌中ErbB2过表达与血管内皮生长因子A,C和D的高表达关系。方法:用免疫组织化学方法检测107例乳腺癌患者和22例良性肿瘤中ErbB2、VEGF-A、VEGF-C和VEGF-D的表达。结果:VEGF-C和VEGF-D在乳腺癌的表达率高于乳腺的良性肿瘤,然而,VEGF-A、VEGF-C和VEGF-D的表达与erbB2的表达呈显著正相关,VEGF-A高表达而生存率降低,乳腺癌患者中 VEGF-C 或者VEGF-D高表达时,生存率更低,但是没有统计学意义,然而VEGF -A和VEGF-C联合表达水平比单一表达更有统计学意义。结论:血管与淋巴管的表达与ErbB2有一定的关系,VEGF-A和VEGF-C的同时表达对患者预后生存率更有价值。
Objective:ErbB2 overexpression correlates with increased expression of vascular endotheliai growth factors A , C,and D in human breast carcinoma. Methods:The expression levels of VEGF -A,VEGF-C,and VEGF-D protein in 107 breast carcinoma cases and 22 nonmalignant breast tissue samples were examined by immunohistochemistry Results:Higher expression of VEGF -C and VEGF-D was found in breast carcinomas than in nonmal ignant breast tissue samples .Moreover,expression of VEGF-A, VEGF-C,and VEGF-D was significantly and positively correlated with ErbB 2 expression.High levels of VEGF -A expression were associated with shorter disease-free survival ( DFS) .Patients with tumors expressinghigh levels of VEGF -C or VEGF-D showed a notable trend for worse DFS , however ,it was not statlsticallysignificant .The combination of VEGF-A and VEGF-C status predicted survival better than either marker-alone.Conclusion:Our study suggests that expression of the angiogenic and lymphangiogenic factors (i.e.,VE

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目的:探讨TBX2、FEZ1在正常胃黏膜、上皮内肿瘤及胃腺癌中的表达及其临床病理意义。方法采用免疫组织化学方法检测30例正常胃黏膜、30例上皮内肿瘤及80例胃腺癌中 TBX、FEZ1蛋白质的表达。应用统计学软件 SPSS15.0分析其与临床病理及预后的关系。结果 TBX2在低分化组织中的阳性表达高于高分化组织(P<0.05),临床分期Ⅳ期的表达高于Ⅰ期(P<0.05),肌层浸润>1/2的表达高于≤1/2的表达(P<0.05);FEZ1在低分化组织中的阳性表达低于高分化组织(P<0.05),临床分期Ⅲ期的阳性率表达低于Ⅰ期的表达(P<0.05),肿瘤直径>4cm阳性表达低于≤4cm的表达(P<0.05),肌层浸润>1/2的表达低于≤1/2的表达(P<0.05),有淋巴结转移的表达低于无淋巴结转移的表达(P<0.05),年龄>60岁的表达低于≤60岁的表达(P<0.05)。结论TBX2和 FEZ1在胃黏膜发生发展中发挥着重要作用,前者发挥着原癌基因的作用,后者发挥着抑癌基因的作用。
Objective To investigate the expression of T-box protein 2 (TBX2)and anti-oncogene FEZ1 in normal gastric muscosa,intraepithelial tumor and gastric adenocarcinoma and the roles of their clinic pathology.Methods The exprssion of TBX2 and FEZ1 were detected by immunohistiochemistry in normal gatromucosa,intraepithelial tumor and gastric carcinoma. Statistic soft ware SPSS1 5 .0 was used to analyze the clinicopathological significance and its relationship to prognosis.Results The positive expression rate of TBX2 protein was higher in low differentiated group than in high differrentiated one (P<0.05),and TBX2 in group with stageⅣwas also higher than in group with stageⅠ(P<0.05),and also in group with over one half deeply muscle infiltration was significantly higher than in group with under one-half infiltration (P<0.05).The positive expression rate of FEZ1 protein was lower in low differentiated group than in high differrentiated one (P<0.05),so was it in group with tumor diame

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目的探讨恒温快速冷冻切片对乳腺癌组织中Cerb B-2蛋白表达的影响。方法应用Max Vinsion免疫组织化学技术,对130例浸润性导管癌患者的恒温冷冻切片对应组织和剩余组织石蜡标本进行标记,比较两组Cerb B-2蛋白表达情况。结果 130例浸润性导管癌冰冻切片对应组织样本组中42例Cerb B-2表达阴性,33例Cerb B-2表达+,34例Cerb B-2表达++,21例Cerb B-2表达+++;剩余组织常规切片样本组56例Cerb B-2表达阴性,50例Cerb B-2表达+,19例Cerb B-2表达++,5例Cerb B-2表达+++;两组标记结果比较具有非常显著性差异,而且两组Cerb B-2阳性表达结果一致性较差。结论恒温快速冷冻切片影响乳腺癌组织中Cerb B-2蛋白表达,减弱乳腺癌组织中Cerb B-2蛋白阳性表达
Objective To explore the influence of frozen section on the expression of CerbB-2 in patients with advanced breast cancer .Methods The CerbB-2 expression of tissues after frozen section and the rest tissues in 130 cases of advanced breast cancer were detected by Max vinsion immunohistochemistry ,and the difference of the 2 groups were compared .Results There ware 42 cases of CerbB-2 negative expression ,33 cases of CerbB-2+expression ,34 cases of CerbB-2++expression and 21 cases of CerbB-2+++expression in the frozen section group .There were 56 cases of CerbB-2 negative expression ,50 cases of CerbB-2+expression,19 cases of CerbB-2++expression and 5 cases of CerbB-2+++expression in the rest tissues by routine section .The difference of the CerbB-2 expression of the 2 groups was significant , and the CerbB-2 expression of the 2 groups had low degree of consistency .Conclusion The frozen section affects the CerbB-2 expression in the advanced breast cancer,and decreases the Cerb
目的:探讨结直肠癌组织中干扰素诱导蛋白16(IFI16)表达与p53及p21waf1/cip表达间的相关性。方法免疫组化SP法检测56例不同病理组织分化结直肠癌标本IFI16、p53、p21waf1/cip的蛋白表达,Pearson等级相关分析法分析及其表达间的相关性。结果在结直肠癌细胞中IFI16除呈现胞核表达外,尚存在胞质的异位表达。 IFI16的表达与p21waf1/cip表达呈正相关(r=0.365,P<0.01),而异位的IFI16与p21waf1/cip表达呈负相关(r=-0.298,P<0.05);IFI16的表达与p53表达无显著相关性(r=0.056,P>0.05)。结论 IFI16可能通过细胞表达及定位调控p21waf1/cip及其下游路径激活参与结直肠癌的发生、发展。
Objective To study the expression and significance of IFI 16, p53 and p21waf1/cip in colorectal cancer tis-sues.Methods The SP immunohistochemistry method was applicated to detect the expression of IFI 16, p53 and p21waf1/cip in paraffin embedded blocks of colorectal cancer tissues .Results IFI16 was detected in nuclear and cytpoplasm of color-ectal cancer cell.The expression of IFI16 was positive correlated with p21waf1/cip (r=0.365, P 0.05).Conclusion IFI16 might take part in the occurrence progress of colorectal cancer ac-cording to the expression and location of IFI 16 related with the p21waf1/cip .

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探讨小细胞肺癌c-myc癌基因的表达对Nrf2表达的影响,分析c-myc与Nrf2表达的相关性。方法:选用两株耐药性不同的肺小细胞癌肿瘤细胞株,小细胞肺癌药物敏感株H69及耐药株H69AR,Western-Blot方法比较Nrf2,c-myc在两株细胞间的表达差异;H69细胞通过Lipofectamine2000脂质体转染过表达c-myc,Western-Blot方法检测过表达c-myc前后Nrf2蛋白表达变化;H69AR细胞通过Lipofectamine2000脂质体转染c-myc siRNA,Western-Blot方法检测c-myc siRNA前后Nrf2的蛋白表达变化。结果:与H69细胞相比,H69AR细胞株中c-myc及Nrf2高表达;H69细胞c-myc转染后,Nrf2表达上调;H69AR细胞c-myc siRNA干扰后,Nrf2表达下调。结论:小细胞肺癌中Nrf2的表达与c-myc表达呈正相关。
Objective:To determine the influence of oncogene c-myc over Nrf2 in small cell lung cancer, and to an-alyze the correlation between c-myc and Nrf2 expressions. Methods:Two lines of small-cell lung cancer cells were chosen in this study. One is drug sensitive H69 cell. The other is multidrug resistant H69AR cell. Western blot assays were uti-lized to compare the different expression levels of c-myc and Nrf2. Overexpression of c-myc by Lipofectamine 2000 trans-fection was performed in H69 cells, and Nrf2 protein expression was compared between the control and c-myc transfection groups. Down-regulation of c-myc by siRNA transfection was performed in H69AR cells, and Nrf2 protein expression was compared between the control and c-myc siRNA groups. Results: Compared with H69 cells, protein expression level of c-myc and Nrf2 was much higher in H69AR cell. In H69 tumor cells, Nrf2 was up-regulated after c-myc transfection. Mean-while, in H69AR tumor cells, Nrf2 was down-regulated after c-myc s

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