目的 探讨低频超声辐射是否增加化学治疗(化疗)药物对人肺癌细胞的凋亡作用.方法 将人肺癌A549的细胞分成4组:空白对照组常规培养;单纯使用顺氯氨铂组(单纯化疗组,药物终浓度5mg/L);单纯低频超声辐射处理组(单纯超声组,频率30 kHz,发射强度2.8 W/cm2,作用时间5 min)以及低频超声辐射联合顺氯氨铂化疗组(超声联合化疗组,超声治疗和化疗方法同单纯超声组和单纯化疗组).经过各个条件处理的细胞培养36 h后应用流式细胞仪检测A549细胞凋亡率.结果 超声联合化疗组A549细胞凋亡率[(7.84±2.74)%]明显高于单纯化疗组[(5.06±0.88)%]、单纯超声组[(2.71±0.99)%]和空白对照组[(2.64±0.99)%],差异均有统计学意义(P=0.012,P=0.004,P=0.010),同时超声联合化疗组及单纯化疗组A549细胞凋亡率也明显高于空白对照组,差异有统计学意义(P =0.004),单纯超声组与空白对照组间A549细胞凋亡率差异无统计学意义(P =0.055).结论 体外低频超声辐射可以增加化疗药物对人肺癌细胞的凋亡作用.
Objective To investigate whether low intensity ultrasound enhances chemotherapeutic efficacy on human pulmonary carcinoma.Methods The human pulmonary carcinoma cell line A549 cell suspension was divided into four subgroups:routinely cultured A549 cells as control group; A549 cell suspension treated with cisplation group (final concentration was 5 mg/L) ; A549 cell suspension treated with low intensity ultrasound group (30 kHz,2.8 W/cm2,for five minutes) ; A549 cell suspension treated with combination of low intensity ultrasound (30 kHz,2.8 W/cm2,for five minutes) and cisplation group (final concentration was 5 mg/L).The A549 cell suspension treated with various treatments was incubated for 36 h.The A549 cell apoptosis ratio was detected using flow cytometry.Results The A549 apoptosis ratio was significantly higher in the combination of low intensity ultrasound and cisplation (7.84 ± 2.74) % than that in the subgroup of cisplation alone (5.06 ± 0.88) % (P =0.012) or low in