目的：改进青霉素V钾片含量测定的方法。方法：色谱条件：Inertis ODS－SP C18柱（150mm×4．6mm，5μm）；流动相为A-B（60：40）[A：pH3．5磷酸盐缓冲液（取0．5m01·L^-1磷酸二氢钾溶液，用磷酸调节pH至3．5）－乙腈．水（10：30：60）；B：pH3．5磷酸盐缓冲液．乙腈－水（10：55：35）]；流速为1．0ml·min^-1；检测波长268nm；柱温：室温；进样量：10tzl。结果：青霉素V钾在0．0329—0．8216mg·ml“范围内呈良好线性关系（r=0．9999）；平均回收率99．9％，RSD为0．6％（n=9）。结论：此法重复性好，结果准确，可用于青霉素V钾、青霉素V钾片及胶囊的含量测定。

Objective:To improve the determination method for penicillin V potassium tablets. Methods:An Inertis ODS-SP C18 column (150 mm × 4. 6 mm, 5 μm) was used. The mobile phase consisted of A and B(60∶40) [ A: pH 3. 5 phosphate buffer(0. 5 mol·L-1 potassium dihydrogen phosphate solution, adjusting pH to 3. 5 with phosphoric acid)-acetonitrile-water (10∶30∶60);B:pH 3. 5 phosphate buffer-acetonitrile-water (10∶55∶35)]. The flow rate was 1. 0 ml·min-1, the detection wavelength was 268nm, the column temperature was room temperature and the injection volume was 10 μl. Results:The linear range of penicillin V potassium was 0. 032 9-0. 821 6 mg·ml-1(r=0. 999 9, and the average recovery was 99. 9%( RSD=0. 6%, n=9). Conclusion:The method is reproducible and accurate, and can be used in the content determination of penicillin V potassium tablets and capsules.

以α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,β-内酰胺类抗生素青霉素V钾为模板,制备了分子印迹聚合物(MIP),通过紫外吸收光谱法研究发现模板分子与单体之间产生了氢键相互作用,二者的结合常数为2.889×109L3/mol3,化学配位比为3。通过吸附实验,探讨了印迹聚合物对模板分子的识别特性,结果印迹因子为2.31,最大表观结合量为8.63μg·mg-1,表明MIP对模板青霉素V钾有较强的特异识别性,为进一步将其应用于实际样品中抗生素的残留分析奠定了基础。

The penicillin V potassium imprinted polymer was prepared by bulk polymerization with methacrylic acid( MAA) as func-tional monomer,ethylene glycol dimethacrylate( EGDMA) as crosslinker,and azoisobutyronitrile( AIBN) as initiator,so that the MIP was targeted to recognize the penicillin VK. According to the ultraviolet spectrum, hydrogen-bonding interactions existed between template molecules and monomers with mole ratio 1:3 and better stabilization(K=2. 889×109L3/mol3). The adsorption and recog-nition ability of MIP for penicillin VK were researched by adsorption experiments. Imprinted factor was 2. 31,and the maximum ap-parent binding constant was 8. 63 μg·mg-1 . The results showed that MIP has high affnity,specific recognition ability and selectivity for penicillin VK.

从疑似发生猪链球菌病患猪的肺脏和淋巴结中分离到1株细菌。该细菌经形态学观察、培养特性、溶血试验、生化试验和动物试验鉴定为偏向厌氧型链球菌。该菌株对小白鼠存在致病性。药敏试验表明:分离菌株对阿莫西林和先锋V高度敏感,对菌必治、呋喃妥因、磺胺类药中敏,对青霉素、链霉素、卡那霉素和恩诺沙星等药物不敏感。

One Streptococcus suis was obtained from the lung and lymphaden of sick swine. The streptococcus suis was identified as deviation oxygen-phobe by observation of morphology, cultural character, hemolysin test, biochemical test and animal experiment,could cause death of the Mouse. Drug susceptibility test showed that Amoxicillin and vanguard V were high susceptible, Rocephin, Nitrofu-rantoin,and Sulfanilamide were middle susceptible,penicillin,Streptomycin,Kanamycin,enrofloxacin base were low susceptible.

在制糖过程中，蔗汁中微生物活动是影响白糖产品品质的一个重要因素。为了消除糖厂有害菌对白糖产品品质的影响，本文主要探索了常用食品防腐剂、天然食品防腐剂、其它试剂以及氯化钠水溶液低压直流电解后对甘蔗压榨过程中危害最严重的明串珠菌类微生物的抑菌效果。实验结果表明，对羟基苯甲酸乙酯钠、注射用青霉素钠、乙二胺四乙酸钠（EDTA-2Na）均能有效抑制糖厂生产中主要的有害菌，其最低有效抑菌浓度分别为0.0016、0.0096、0.0056 g/L；氯化钠水溶液在20 V 直流电压电解的抑菌效果与盐浓度的大小以及延迟时间相关，7%左右的盐溶液配合低压电解的抑菌作用最显著。

In the process of sugar production, the microbial activity in the cane juice is an important factor in damaging the quality of sugar products. In order to inhibit the harmful effect of bacterium on the quality of sugar products, the antibacterial effect of common food preservatives, natural food preservatives, other reagents and sodium chloride aqueous electrolytes solution on Leuconostoc, the most harmful bacterium during the crushing process of cane sugar mills, was explored in this paper. The results showed that sodium p-hydroxybenzoate, ethyl injection of sodium penicillin, sodium ethylenediamine tetraacetate (EDTA-2Na) could inhibit the main harmful bacterium effectively during the process of sugar production and the lowest effective antibacterial concentration was 0.0016 g/L, 0.0096 g/L, 0.0056 g/L respectively. The antibacterial effect of sodium chloride aqueous electrolytes solution at 20 V is related to the salt concentration and the delay time, and the antibacterial

目的比较注射与口服青霉素类药物不良反应的差别。方法采用回顾性分析方法对2007~2012年江苏省不良反应中心收集到的青霉素类药品的不良反应报告进行分析。结果江苏省注射用青霉素的不良反应数为6 082例,占92.98%;口服青霉素的不良反应数为454例,占6.94%。注射用青霉素有新的严重的不良反应2例。注射用青霉素不良反应病例经治疗后有9例死亡病例,3例病人留下后遗症;口服青霉素的不良反应经治疗后均痊愈或好转。结论口服青霉素类药物不良反应数量少,且严重程度低,经治疗后无死亡或留下后遗症的病例。

Objective Compare with injection penicillin drugs and oral penicillin drugs for ADR .Method The adverse reaction re-ports of penicillin drugs collected by Jiangsu adverse reaction center from 2007 to 2012 were analyzed retrospectively .Result The case number of adverse reactions of injection penicillin was 6 082 ,accounting for 92 .98% ;Oral penicillin adverse reactions num-ber was 454 cases ,accounting for 6 .94% ;The injection penicillin drugs had new serious adverse reactions in 2 cases ;After the treatment of injection penicillin adverse reaction ,9 cases were die and 3 cases left sequela ;On the other hand ,the cases of oral penicillin adverse reactions were recoveried or better after treatment .Conclusion For the adverse drug reactions of oral penicillin , the quantity was less and the serious degree was low and no death or sequela was reported after treatment .