以罗马生菜种子为材料,研究了6种不同比例的红（R）蓝（B）光质（2R/1B,4R/1B,8R/1B,1R/2B,1R/4B,1R/8B）对生菜种子萌发及幼苗生长的影响。结果表明：与对照的白光相比,以红光为主的复合光处理（2R/1B,4R/1B,8R/1B）促进种子萌发效果明显,其发芽势、发芽指数和活力指数均显著高于对照。红蓝复合光对生菜幼苗的形态建成、根系活力、叶片色素含量和叶绿素荧光均有促进作用,以红光为主的复合光处理促进效果优于以蓝光为主的复合光处理。在以红光为主的复合光处理中,2R/1B,4R/1B处理对生菜种子萌发及幼苗生长的促进效果优于8R/1B。总之,不同红蓝光比例显著影响了生菜种子萌发及幼苗生长,其中以2R/1B和4R/1B处理最优。

The study investigated the effects of light qualities on seed germination and seedling growth of lettuce . Plants were grown under six different combinations of red and blue light (2R/1B, 4R/1B, 8R/1B, 1R/2B, 1R/4B, 1R/8B).The results showed that different light qualities had different influences on seed germination and seedling growth of lettuce .The combination of red ( R ) and blue ( B ) lights based on red promoted seed germination obviously, and the germination potential , germination index and vigor index under these treatments were significantly higher than those of control .Compared with seedlings grown under white light , the mixture of blue and red light promoted the morphogenesis , root activity , content of leaf pigment and chlorophyll fluorescence of lettuce seedlings . Furthermore, the promoting effects under the RB lights based on red were better than those of treatments based on blue.The combination of 2R/1B and 4R/1B were better than the combination of 8B/1R on promoti

对渤海湾北岸孔深600m的Bg10孔进行了详细的沉积学和古地磁研究,划分了39个沉积单元;基于516个样品(60个交变样品,456个热退样品)分离出的特征剩磁,建立了钻孔的磁性地层,并与标准极性年表相对比,确定正极性N1、N2、N3、N4、N5、N6和N7分别对应C1n、C1r.1n、C2n、C2r.1n、C2An.1n、C2An.2n和C2An.3n,R1、R2、R3、R4、R5和R6分别对应C1r.1r、C1r.2r+C1r.3r、C2r.1r、C2r.2r、C2An.1r和C2An.2r。钻孔B/M界限埋深162.3m,M/G界限埋深为475.8m,钻孔底部位于C2An.3n内,其年龄应小于3.596Ma。Bg10孔包括3个沉积阶段:Ⅰ阶段为3.3~3.6Ma,沉积速率略大于130m/Ma,为构造活动期,唐县期夷平面结束后,盆地沉降和山体隆升的差异增大,并与西部地区的青藏运动A幕相对应;Ⅱ阶段为1.9~3.3Ma,沉积速率为112m/Ma,为盆地的填充过程,气候变化控制沉积过程,但沧县隆起和黄骅坳陷之间沉降仍有差异。Ⅲ阶段为0~1.9Ma,沉积速率为202m/Ma,约在1.0~1.2Ma发生一期构造活动,与西部地区的昆黄运动时代大体相当。1.0Ma以来,沉积过程受构造和气候变化双重影响,沧县隆起和黄骅坳陷之间仍有差异沉降。唐山-邢台断裂带可能在1.9Ma之后活动性增强,并和张家口-蓬莱断裂带在沧县隆起上的交汇区域形成一个次级凹陷。

Based on detailedsedimentological and magnetostratigraphic study of Bg10 borehole (600m)in the northern coast of Bohai bay,there are 39 sedimentary units and 8 normal magnetozones and 7 reverse magnetozones according on 516 specimens (456 specimens subjected to progressive thermal demagnetization and 60 specimens subjected to alternating field demagnetization ) calculated the characteristic remanent magnetization (ChRM)directions.Thinking about sedimentation rates of different magnetozones,Comparing to the geomagnetic polarity timescale (Cande et al.,1995;Gradstein et al., 2004),the normal magnetozones N1,N2,N3,N4,N5,N6 and N7 can be correlated with C1n,C1r.1n, C2n,C2r.1n,C2An.1n,C2An.2n and C2An.3n,the reverse magnetozones R1,R2,R3,R4,R5 and R6 can be collated with C1r.1r,C1r.2r+C1r.3r,C2r.1r,C2r.2r,C2An.1r and C2An.2r.The B-M and M-G boundary of borehole are located in 162.3m and 475.8m respectively,and the age of the bottom is less than 3.596Ma.There have been three sedimentation ph

利用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱（Sephadex LH-20）、MCI柱色谱、中压ODS柱色谱及高效液相色谱（HPLC ）等分离手段，并借助核磁共振等多种波谱学方法，从昆明山海棠（Tripterygium hypoglaucum）茎叶中共分离鉴定了11个化合物，结构类型包括木脂素、甾体以及酚性成分，分别为杜仲树脂酚（1），（7R，7′R，7″R，8S，8′S，8″S）-4，4″，7″，9″-四羟基-3，3′，3″，5，5′，5″-六甲氧基-7，9′；7′，9；4′，8″-氧代-8，8′-倍半木脂素（2），（7R，7′R，7″S，8S，8′S，8″S）-4，4″，7″，9″-四羟基-3，3′，3″，5，5′，5″-六甲氧基-7，9′；7′，9；4′，8″-氧代-8，8′-倍半木脂素（3），（7R，7′R，8S，8′S）-4，4″，7″，9″-四羟基-3′，5，5′，5″-四甲氧基-7，9′；7′，9；4′，8″-氧代-8，8′-倍半木脂素（4），（7R，7′R，8S，8′S）-4，4″，7″，9″-四羟基-3′，3″，5，5′，5″-五甲氧基-7，9′；7′，9；4′，8″-氧代-8，8′-倍半木脂素（5），20β-β-D-葡萄糖-3-羰基孕甾-4-烯（6），20α-β-D-葡萄糖-3-羰基孕甾-4-烯（7），β-谷甾醇（8），邻羟基苯甲醇-1-O-β-D（3′-苯甲酰基）葡萄糖苷（9），邻羟基苯甲

By various kinds of isolation and purification methods and spectroscopic evidences,1 1 compounds are isolated and identified from the stems and leaves of Tripterygium hypoglaucum,including lignans,steroids and phenolic compounds.These known compounds are elucidated as (+)-medioresinol(1),(7R,7′R,7″R,8S,8′S,8″S)-4,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′;7′,9;4,8″-oxy-8,8′-sesquineolignan-7″,9″-diol(2),(7R,7′R,7″S,8S,8′S,8″S)-4,4″-dihydroxy-3,3′,3″,5,5′,5″-hexamethoxy-7,9′;7′,9;4,8″-oxy-8,8′-sesquineolignan(3),(7R,7′R,8S,8′S)-4,4″,7″,9″-tetrohydroxy-3′,5,5′,5″-tetramethoxy-7,9′;7′,9,4,8″-oxy-8,8′-sesquineolignan(4),(7R,7′R,8S,8′S)-4,4″,7″,9″-tetro-hydroxy-3′,3″,5,5′,5″-pentamethoxy-7,9′;7′,9,4,8″-oxy-8,8′-sesquineolignan(5),20β-β-D-glucopregn-4-en-3-one(6),20α-β-D-glucopregn-4-en-3 -one(7),β-sitosterol(8),salicyl al-cohol-1 -O-β-D(3′-benzoyl)glucopyranoside(9),2-hydroxy-benzene-methanol(10),4-hydroxy-2-methoxy-be

目的探讨金属抗性促生菌对锌污染土壤中玉米生长和Zn吸收的影响，为利用微生物缓解重金属对农作物胁迫和减少重金属吸收提供理论依据。方法通过盆栽试验研究接种促生菌Ⅱ2 R3和Ⅳ8 R3是否能够缓减Zn对玉米的毒害作用，减少玉米对Zn的吸收和积累。结果和结论促生菌Ⅱ2 R3、Ⅳ8 R3不仅有很强的抗Zn胁迫能力，而且具有较强地吸附、积累Zn的能力。在中、轻度Zn污染（200和400 mg
83; kg －1）土壤上，单独接种促生菌Ⅳ8 R3促进了玉米的生长；当土壤Zn污染水平达到800 mg
83; kg－1时，同时接种促生菌Ⅳ8 R3＋Ⅱ2 R3显著促进玉米的生长。单独或同时接种促生菌Ⅳ8 R3、Ⅱ2 R3使不同Zn污染水平土壤中的有效Zn含量均显著降低。当土壤Zn含量达到800 mg
83; kg－1时，单独接种菌株Ⅱ2 R3或同时接种Ⅱ2 R3＋Ⅳ8 R3均显著抑制玉米对Zn的吸收和向地上部转运，而在中、轻度Zn污染（Zn≤400 mg
83; kg－1）土壤上，3种接种处理对玉米吸收Zn均没有显著影响。表明在高锌污染（800 mg
83; kg－1）土壤中，接种促生菌Ⅳ8 R3、Ⅱ2 R3能显著抑制玉米对Zn的吸收和向地上部转运；菌株Ⅳ8 R3、Ⅱ2 R3对玉米生长的影响与土壤Zn污染水平和菌株的性质有关。

Objective]Effects of metal resistant plant growth promoting bacteria ( PGPB ) on corn growth and Zn accumulation were studied to provide a fundamental groundwork for mitigating the stress of heavy metal on crop growth and decreasing heavy metal uptake .[Method] A pot experiment was carried out to study the effects of inoculation with two Zn resistant PGPB ,Ⅱ2 R3 andⅣ8 R3 on corn plant growth and Zn uptake from Zn contaminated soils (0, 200, 400 and 800 mg· kg -1 Zn addition).[Result and conclu-sion] The strains Ⅱ2 R3 and Ⅳ8 R3 not only grew well with different concentrations of Zn , but also ab-sorbed or accumulated much higher Zn from the growing medium .Individual inoculation of strain Ⅳ8 R3 to the soil with 200 or 400 mg· kg -1 Zn addition both promoted the corn growth .For the soils with 800 mg· kg -1 Zn addition , simultaneous inoculation with strains Ⅱ2 R3 andⅣ8 R3 promoted the corn growth . Individual or simultaneous inoculation with strains Ⅱ2 R3 andⅣ8 R3

从肖尔布拉克酒业酱香型高温大曲中通过常温法和热处理法分离出了39株细菌,采用麸皮浸出汁培养基发酵,发现其中15株细菌的发酵液酱香味明显。将这15株细菌进行复发酵并进行蛋白酶活力的测定,显示15株菌株均有蛋白酶活力,其中有6株活力较强,分别为C1-3,C4,C8,R12,R19和R20。对这6株菌株进行基因组测序,C1-3、C4、R19和R20为枯草芽孢杆菌,C8为甲基营养型芽孢杆菌,R12为考克氏菌。对该6株菌发酵液进行气相色谱质谱分析,结果显示,6株菌检测出的物质数分别为:8种、19种、24种、15种、8种和9种。其中C1-3和R19生成的四甲基吡嗪含量较高。

39 strains of bacteria were isolated from Xiaoerbulake Jiangxiang high-tem perature Daqu by normal temperature treatment and heat treatment. Fermented in wheat-extract medium, 15 of these strains produced obvious Jiangxiang flavor. The 15 strains went through fermentation process again and their protease activity were determined. All 15 strains had protease activity, and 6 of them (C1-3, C4, C8, R12, R19 and R20) had relatively high protease activity. Through 16S rDNA sequence analysis, strains C1-3, C4, R19, and R20 were identified as Bacillus subtilis, C8 as Bacillus methylotrophicus, and R12 as Kocuria sp. Then the fermentation liquid of the 6 strains went through GC-MS analysis. The results showed that the numbers of components generated were 8, 19, 24, 15, 8 and 9, respectively, and that strains C1-3 and R19 generated more Tetramethylpyrazine than other strains.