目的：观察硫酸镁对促肾上腺皮质激素（ACTH）治疗婴儿痉挛症（IS）过程中高血压的预防作用。方法选取2011年5月至2013年10月住院的46例IS患儿，以入住医院分为硫酸镁组23例，给予硫酸镁联合ACTH治疗；对照组23例，单用ACTH治疗，疗程均为2周。观察比较两组高血压的发生率。结果硫酸镁组患儿没有出现高血压，全部完成疗程；对照组出现6例高血压，18例完成疗程。硫酸镁组在高血压的发生及疗程完成方面均优于对照组，差异有统计学意义（P〈0.05）。结论硫酸镁能预防ACTH在治疗IS过程中高血压的发生，有利于完成疗程，尽早控制病情。

Objective To examine the preventive effect of magnesium sulfate on hypertension caused by ACTH in the treatment of infantile spasms (IS). Methods 46 children diagnosed as IS were recruited from two hospitals during May, 2011 to October, 2013.23 patients in group A (treatment group) were treated with magnesium sulfate and ACTH in hospital A;another 23 cases in group B (control group) were treated with ACTH only in hospital B. The therapy course was 2 weeks. Results Hyperten-sion was not observed in the treatment group, while 6 children were observed with hypertension in the control group. There was signiifcant difference between the two groups (P<0.05). Conclusions Magnesium sulfate could prevent the incidence of hyper-tension in the treatment of IS with ACTH, and beneift the completion of treatment course.

目的探讨糖皮质激素(GC)、促肾上腺皮质激素(ACTH)在肾病综合征复发患儿中的疗效及安全性。方法选取激素敏感型原发性肾病综合征患儿为研究对象,其中95例患儿在停用激素后复发或治疗期间复发,将重新采用糖皮质激素治疗或上调激素剂量治疗的患儿纳入GC组,采用ACTH治疗的患儿纳入ACTH组,观察治疗前与治疗期间两组患儿激素用量、临床疗效、不良反应及肾上腺皮质功能变化。结果 ACTH组35例(83.3%)治疗期间未再复发,7例(16.7%)患者因感染导致频繁复发,治疗无效。GC组41例(77.4%)治疗有效,12例(22.6%)单纯激素治疗效果不明显。ACTH组与GC组总有效率无显著差异(P0.05)。ACTH组患儿GC用量显著高于GC组(P0.05)、身高增加显著高于高于GC组,体重增加显著高于低于GC组、肾上腺皮质功能降低显著低于GC组(P均0.05)。结论 ACTH方案治疗原发性肾病综合征复发患儿效果肯定,可降低糖皮质激素使用剂量、改善患儿生长发育的抑制、减轻避免肾上腺皮质功能降低。

Objective To investigate the efficacy and clinical significance of adrenocorticotropic hormone on the treatment of children with relapsed primary nephrotic syndrome( PNS) . Methods Children enrolled in this study all met the diagnostic criteria of PNS char-acterized by steroid-dependent and relapse. These children were assigned into GC group and ACTH group based on different treatments. The hormone dose,clinical efficacy,adverse reactions and adrenocortical function were compared between two groups. Results Thirty-five children(83. 3%) had improved and 7 children(16. 7%) relapsed due to infection in ACTH group, while forty-one children (77. 4%) had improved and 12 children(22. 6%) relapsed in GC group. The efficacy had no obvious difference between two groups (P>0. 05). The dosage of hormone used in GC group was higher than ACTH group during treatment(P<0. 05). In ACTH group dos-age of hormone could be successfully reduced till stop of using. The ratio of height and the ratio of weight

目的探讨三叶因子3(TFF3)在腺垂体远侧部嗜酸性细胞和嗜碱性细胞中的表达,明确TFF3在远侧部的分布。方法采用相邻切片的免疫组织化学染色,在相邻切片上分别显示TFF3/生长激素(GH)、TFF3/催乳素(PRL)、TFF3/促甲状腺激素(TSH)、TFF3/促肾上腺皮质激素(ACTH)、TFF3/卵泡刺激素(FSH)、TFF3/黄体生成素(LH)的表达。结果 TFF3和各嗜色细胞的免疫反应产物为棕黄色,主要位于细胞质,ACTH免疫阳性信号在胞膜也有表达,主要分布在腺垂体的远侧部。邻片显示TFF3存在于部分GH、PRL、TSH、ACTH、FSH、LH细胞,分别占19.4%、22.4%、9.2%、6.5%、35.7%、8.3%,以FSH最多,PRL、GH次之。结论垂体远侧部TFF3可分别表达于GH、PRL、TSH、ACTH、FSH、LH细胞。

Objective To investigate the distribution and expression of trefoil factor 3(TFF3) in the eosinophilic cells and basophilic cells in the pars distalis of the rat pituitary .Methods The immunohistochemiscal staining technique was used to show the coexpression of TFF3/growth hormone (GH),TFF3/prolactin (PRL),TFF3/thyrotroph (TSH), TFF3/adrenocorticotropin (ACTH),TFF3/follicle stimulating hormone (FSH),TFF3/luteinizing hormone (LH) in two contiguous slices .Results The immunoreactive products of TFF 3 and chromophil cells were brown granules , mainly expressed in cytoplasm .ACTH positive signals were expressed in the cell membrane and mainly located in the pars distalis hypophyseos.TFF3 existed in parts of GH,PRL,TSH,ACTH,FSH and LH cells in contiguous slices, accounting for 19.4%, 22.4%, 9.2%, 6.5%, 35.7%, 8.3%, respectively , in which FSH was the most , PRL and GH were less . Conclusion TFF3 expresses in GH, PRL, TSH, ACTH, FSH, and LH cells in the pars distalis , which enriches the mor

目的：探讨大鼠脑梗死后急性期肾上腺皮质功能的改变以及地塞米松的干预作用。方法健康雄性SD大鼠60只，随机分为假手术组（对照组）、模型加生理盐水组（安慰剂组）和模型加地塞米松组（治疗组），每组20只。采用改进的线栓法建立大鼠脑缺血模型。治疗组应用小剂量地塞米松（0.8 mg/kg）替代治疗。各组分别于脑损伤后第3、12、24、72 h 4个时间点进行观察。给予小剂量促肾上腺皮质激素（ACTH）行刺激试验，应用ELISA法测定血清皮质酮（CORT）和ACTH含量。结果安慰剂组大鼠脑损伤后3 h外周血CORT和ACTH均明显高于对照组（P＜0.01），随后逐渐降低，72 h又有升高趋势；各时间点ACTH刺激后CORT上升值均低于对照组（P＜0.05或P＜0.01）。治疗组伤后所有时间点CORT均高于安慰剂组（P＜0.01或P＜0.05），ACTH仅24 h高于安慰剂组（P＜0.05）；各时间点ACTH刺激后CORT上升值均高于安慰剂组（P＜0.05或P＜0.01）。结论大鼠脑梗死急性期即存在肾上腺皮质功能不全，随着时间推移，肾上腺储备功能下降，对ACTH反应程度降低。小剂量短疗程肾上腺糖皮质激素可增强肾上腺皮质对ACTH刺激的敏感性。

Objective To study the changes in adrenal cortex function and protective effect of dexamethasone following acute cerebral infarction in rats. Methods 60 healthy male SD rats were randomly divided into three groups with 20 rats in each group: sham-operated group (control group), model group plus physiologic saline (placebo group) and model group plus dexamethasone (treatment group). The rat model was established by modified thread occlusion. A low dose of dexamethasone (0.8 mg/kg) was injected into the abdominal cavity 10 minutes after cerebral infarction. The related parameters were detected at four time points, 3 hours, 12 hours, 24 hours and 72 hours after cerebral infarction. The plasma ACTH and corticosterone (CORT) levels were measured after stimulated by 1 μg ACTH. Results The levels of ACTH and CORT at 3 hours of placebo group were increased remarkably compared to the control group (P<0.01), then reduced gradually and raised again at 72 hours post injury. The levels o

目的探讨抑郁症患者褪黑素(MT)水平对下丘脑-垂体-肾上腺轴(HPA)功能的影响。方法对86例抑郁症患者,检测血清MT、促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)、皮质醇(COR),并分析其相互关系。结果1以MT中位数(51.3ng/L)为切点,将所有抑郁症患者分为MT高值组(51.3ng/L,n=43)与MT低值组(51.3ng/L,n=43),前者血清CRH、ACTH、COR均显著低于后者(t=3.330,3.315,2.314;P0.01,0.01,0.05);2血清MT水平与CRH、ACTH水平负相关(r=-0.414,-0.329;P0.01,0.05)。结论褪黑素对抑郁症患者的HPA轴功能可能有一定的抑制作用。

Objective To study the effects of melatonin(MT) on activity of hypothalamic -pituitary-adrenal(HPA) axis in depres-sion.Methods Major depression patients(n=86) were rolled for the study and assigned to be tested by MT ,corticotrophin releasing hormone(CRH),adrenocorticotrophin hormone (ACTH),cortisol(COR) and 17-items hamilton depression scale for analysis .Results Divided by the median of serum MT level (51.3ng/L),the patients who had higher MT level (>51.3ng/L) had significantly decreased level of CRH,ACTH,COR (t=3.330,3.315,2.314;P<0.01,0.01,0.05) than those who had lower MT level(<51.3ng/L,n=43). The serum level of MT was markedly related with CRH (r=-0.414,P<0.01) or ACTH(r=-0.329,P<0.05).Conclusion Melatonin may inhibit the activity of hypothalamic -pituitary-adrenal axis in patients with depression .