An experiment was conducted to determine to dilution plate method for transfer AmGS cold-toler-ant gene Photinia×fraseri microbial species and quantity of rhizosphere soil,and bacterial,fungal strains were cultured traits were analyzed,the results showed that:transfer AmGS gene Photinia×fraseri compared with the control group,no significant change bacterial species,fungi species and number did not change;The genera of Trichoderma were dominant in fungi communities and Bacillus in bacterial communities. The transgenic Photinia×fraseri had no evident effect on soil microbial diversity.