Objective To study the effect of different testing methods on patients with chronic liver disease. Methods 80 cases of patients with chronic liver disease of in our hospital from 2012 to 2014 were carried on the discussion and analysis, and used four kinds of test methods, which were colloidal gold immunochromatographic assay, serum HA detection, polymerase chain reaction (PCR quantitative detection) and enzyme-linked immunosorbent assay (ELISH), then discussed the results. Results After four tests, the results show that, GIA detection rate was 90%, the serum HA detection rate was 60%, PCR detection rate was 95%, ELISH detection rate was 50%, GIA and PCR detection rate than ELISH and serum HA detection rate were higher, with higher sensitivity. Conclusion The effect of GIA, PCR detection in patients with chronic liver diseases is better, and recommend using the two detection methods in clinical practice.