研究17β-雌二醇(17β-E2)对子宫内膜癌细胞发生上皮间质转化的诱导作用。方法:用17β-E2处理Ishikawa和HEC-1A细胞后,Western blot法检测两种细胞中E-cad、N-cad和Vimentin蛋白的表达。结果:经10-8mol/L E2作用后,Ishikawa细胞中的E-cad蛋白表达减少(P0.05),N-cad、Vimentin蛋白增加(P0.05);其他浓度作用前后,各蛋白表达无明显变化(P0.05)。经不同浓度E2作用后,HEC-1A细胞中E-cad、Ncad、Vimentin蛋白均无明显变化。结论:17β-E2仅在特定浓度下存在诱导ER阳性子宫内膜癌细胞Ishikawa发生上皮间质转化的作用。

Objective:To make clear wether 17β-E2 can induce EMT in the endometri-al carcinoma cell. Methods:Ishikawa and HEC-1A cell were cultured by 17β-E2 ,then the ex-pressions of E-cad、N-cad and Vimentin protein were detected by Western blot analysis. Re-sults:The E-cad protein became less(P<0. 05) while the N-cad,Vimentin protein got more (P<0. 05),in the Ishikawa cell which was influenced by 10-8mol/L 17β-E2. The others concentri-on did not change the expression of the proteins. In HEC-1A cell,the E-cad、N-cad and Vimen-tin protein didn''t get different when affected by E2 . Conclusions:The 17β-E2 can only promote the epithelial-mesenchymal transition in the Ishikawa cell in certain concentration. But it could not produce epithelial-mesenchymal transition in the HEC-1A cell.

目的：观察上皮间质转化相关标记物E-钙黏蛋白（E-cadherin）和Vimentin基因及其蛋白在食管鳞状细胞癌淋巴结转移组和非转移组中的表达，并分析E-cadherin和Vimentin表达的相关性。方法采用免疫组织化学SP法和实时定量逆转录聚合酶链反应（qRT-PCR）检测47例有淋巴结转移、30例无淋巴结转移的食管癌和20例正常食管组织中E-cadherin和Vimentin基因及其蛋白的表达情况。结果对照组、非转移组与转移组 E-cadherin蛋白表达分别为20/20（100％）、29/30（96．67％）和44/47（93．62％），组间比较差别有统计学意义（ P＜0．05）。转移组的高表达率22/47（46．8％）低于非转移组18/30（60％），但差别无统计学意义（ P＞0．05）。Vimentin蛋白在对照组、非转移组、转移组阳性表达率逐渐增高，分别为0/20（0）、11/30（36．67％）、37/47（78．72％），组间比较差别有统计学意义（ P＜0．05），转移组与非转移组间比较差别有统计学意义（ P＜0．05）。Vimentin和E-cadherin表达呈显著负相关，相关系数（ r＝-0．377）。qRT-PCR检测显示非转移组和转移组中E-cadherin和Vimentin mRNA表达量与对照组比较差别有统计学意义（P＜0．05）。E-cadherin基因的表达在转移组（0．43±0

Objective To identify the differences of epithelial mesenchymal transition (EM T ) re-lated biomarkers of E-cadherin and Vimentin in mRNA and protein expression levels between esophageal squamous cell carcinoma (ESCC) with lymph node metastasis and no lymph node metastasis . Methods Immunohistochemistry SP method and quantitative reverse transcriptase real-time polymerase chain reac-tion (qRT-PCR) array were applied to detect mRNA and protein expression levels of E-cadherin and Vim-entin on 47 cases of ESCC with lymph node metastasis and 30 cases of ESCC without lymph node metasta-sis ,and 20 cases of normal esophageal mucous membrane tissues . Results The percentage of expressing E-cadherin in normal control group ,non metastasis and metastasis group of ESCC were 20/20 (100% ) , 29/30 (96 .67% ) ,and 44/47 (93 .62% ) respectively ,there was a statistically significant difference among three groups ( P 0 .05) . The number of cases with the positive Vimentin protein expression

目的：研究 PLK1（Polo-like kinase 1）对食管鳞癌细胞株 TE-15上皮间质转化（epithelial-mesenchymal transition， EMT）的影响，并探讨其作用机制。方法Western blot 检测PLK1稳定过表达的食管鳞癌细胞克隆与空载对照克隆中EMT 相关标志蛋白 E-钙粘素（E-cadherin）、波形蛋白（vim-entin）的表达情况；Real-time PCR 检测 vimentin mRNA 表达水平；分别提取细胞总蛋白和胞核蛋白，Western blot 检测PLK1对信号通路分子β-连环蛋白（β-catenin）表达的影响；采用 RNA 干扰技术，在 PLK1过表达克隆中分别瞬时转染靶向β-catenin 的 siRNA 和非特异性 siRNA，Western blot 检测细胞中 vimentin 的表达；通过免疫沉淀和 Western blot 检测 PLK1对β-catenin 降解复合物的影响。结果与空载对照克隆相比，PLK1过表达的食管鳞癌细胞中，间质标志物vimentin 的表达明显上调，上皮标志物 E-cadherin 的表达明显下调，提示食管鳞癌细胞发生了 EMT；vimentin mRNA 表达水平亦明显升高；在 PLK1过表达的克隆中，细胞总蛋白和胞核蛋白中β-catenin 表达都明显增加，敲降β-catenin 的表达能引起 vimentin 的表达下降；Axin 免疫沉淀物中的 APC及 GSK-3β都明显减少。结论PLK1

Aim To investigate the effect of PLK1 on epithelial-mesenchymal transition (EMT)of human e-sophageal squamous cell carcinoma (ESCC)cells TE-1 5 and its relevant molecular mechanisms.Methods PLK1 overexpressed ESCC cells and control vector were used as the experimental cells.The expression of EMT-related protein markers E-cadherin and vimentin were measured by Western blot.vimentin mRNA was measured by Real-time PCR.Total cellular protein and nuclear protein were respectively extracted,and then they were used to detect the expression of β-catenin by Western blot.β-catenin siRNA and non-specific siR-NA were transiently transfected into the cell clones overexpressed PLK1 ,and then vimentin was detected by Western blot.β-catenin protein degradation com-plex was detected by immunoprecipitation and Western blot.Results The mesenchymal marker vimentin wasdistinctively upregulated and the epithelial marker E-cadherin was distinctively downregulated in the cell clones overexpressed

目的检测颅咽管瘤,特别是肿瘤正常组织交界处上皮间质转化(EMT)相关蛋白(Vimentin、E-cadherin)表达,联系临床病理和预后进行相关分析。方法采用免疫组织化学及免疫荧光双染检测42例颅咽管瘤中Vimentin、E-cadherin表达,联系临床病理特征和预后进行相关分析。结果肿瘤整体和肿瘤正常组织交界处,釉质上皮型和鳞状乳头型颅咽管瘤两亚型间Vimentin、E-cadherin表达均有显著差别。整体范围内,Vimentin和E-cadherin表达与肿瘤复发、术后体质量和下丘脑功能紊乱相关;肿瘤正常组织交界处,Vimentin和E-cadherin表达与肿瘤复发、术后体质量和下丘脑功能紊乱相关。结论颅咽管瘤组织中Vimentin、E-cadherin表达与肿瘤预后相关;EMT有可能是影响釉质上皮型颅咽管瘤进程的重要机制之一。

Objective To assess the different protein expressions of epithelial mesenchymal transition (EM T ) markers Vimentin and E‐cadherin in craniopharyngioma ,especially at the tumour invasive front ,and correlate the findings with clinicopathological fea‐tures and patient outcomes .Methods Forty‐two craniopharyngiomas were subjected to the detection of Vimentin and E‐cadherin by immunohistochemistry and double immunofluorescence staining .The relationships between expression of these markers and various clinico pathological indicators and clinical outcomes of these tumors were analyzed .Results There was statistically significant difference in the expression of Vimentin and E‐cadherin between adamantinomatous and papillary variants in whole tumor and at the tumor invasive front .The expression of Vimentin and E‐cadherin in whole tumor sections were associated with tumor recurrence , postoperative weight and hypothalamic disturbances ,and the expression of vimentin and E‐cad

目的探讨miR-221在耐药胶质瘤细胞中与上皮间质转化的相关性。方法荧光定量PCR和蛋白免疫印迹分析方法比较人胶质瘤细胞株Z1(原发性耐药细胞株)、Z2(药物敏感细胞株)和Z2-BCNU(耐药细胞株)中miR-221、PTEN、p-Akt、Ecadherin、vimentin、MRP1表达的差异。结果 PTEN在Z2细胞中蛋白表达含量明显高于miR-221、vimentin高表达的Z1和Z2-BCNU细胞,在E-cadherin高表达的Z2细胞中vimentin、p-Akt和MRP1含量明显减低。结论 MiR-221通过下调PTEN激活PI3-K/Akt信号从而调控上皮间质转化相关基因的表达。

Objective To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells. Methods The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting. Results The expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin. Conclusion MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.