为了探讨半日花种子与幼苗对干旱胁迫的适应能力,通过对半日花种子进行PEG(聚乙二醇)模拟干旱胁迫处理,以及对半日花幼苗进行盆栽断水干旱胁迫处理,分别研究了干旱胁迫对半日花种子萌发及幼苗生理特性的影响。结果表明:随着干旱胁迫的加剧,半日花种子的萌发率、发芽指数、活力指数均呈下降趋势。10%、20%PEG处理对半日花种子影响较小,25%PEG处理对半日花种子具有明显抑制作用,而30%PEG处理使半日花种子几乎不萌发。随着干旱胁迫的加剧,各指标变化不一,相对含水量呈下降趋势,叶绿素质量分数先上升后下降,脯氨酸质量分数、可溶性蛋白质量分数、丙二醛质量摩尔浓度增加,且在干旱胁迫中后期时变化显著。

Based on the PEG experiment for seeds and pot without water experiment for the seedling , we designed the drought stress simulation experiments of Helianthemum songaricum seeds and seedlings , and studied the effects of drought stress on seed germination and seedling physiological characteristics of Helianthemum songaricum seedlings .The germination rate , germination index and vigor index of Helianthemum songaricum seeds declined with the increasing of PEG solutions .Seed germination was less affected under the 10%and 20%of PEG, and then it was obviously inhibited under 25%, and seeds could hardly germinate under 30%.With the increasing of day numbers without water , the relative water content of leaves declined , the chlorophyll concentration increased firstly and then decreased , and the contents of proline ,soluble protein and MDA increased with obvious variation at the mid-late drought stress stage .

筛选锁阳中具有抗应激作用的提取物,并探讨其可能作用机制。方法:从抗疲劳、抗常压缺氧、耐高温、耐低温4个实验出发考察不同提取物的抗应激效果,并以水提物为研究对象记录小鼠脾脏、胸腺、肾上腺、睾丸脏器指数,并用电化学发光法检测小鼠血浆睾酮及雌二醇水平,用放免法检测小鼠血浆cAMP及cGMP水平。结果:在抗疲劳及耐低温应激试验中锁阳水提物显著提高小鼠存活时间(P0.05,P0.01),并且与人参总皂苷阳性对照比也有所提高。耐高温应激试验中锁阳95%醇提物显著性提高小鼠存活时间(P0.05),锁阳提取物对小鼠抗常压缺氧应激试验中没有显著延长小鼠存活时间(P0.05)。锁阳水提物显著增加小鼠cAMP及cGMP含量,降低cAMP/cGMP的比值,增加雌二醇含量,降低睾酮含量(P0.05)。锁阳水提物能显著降低小鼠睾丸脏器指数(P0.05)。结论:锁阳在延长小鼠抗应激存活时间效果显著,其中水提物对小鼠抗疲劳及耐低温试验方面有效,其机制与降低cAMP/cGMP的比值、增加cAMP含量有关。95%醇提物对小鼠耐高温方面有效。

OBJECTIVE To screen the anti-stress effects of different extracts from Cyrmmorium songaricum and explore its possible anti-stress mechanisms.METHODS The anti-stress effectiveness were investigated from experiments of antifatigue,anti normobaric hypoxia,anti-hyperthermia and anti-hypothermia.The influence to mice organ index of spleen,thymus gland,adrenal glands and testis by aqueous extract were measured.The mice plasma testosterone,estradiol were measured through electrochemiluminescence and cAMP,cGMP were measured through radio-immunity.RESULTS The survival time of mice is longer than positive control group and significantly longer in two Cynomorium songaricum aqueous extracts treated groups of antifatigue and anti-hypothermia than that of blank control group(P 0.05).After treatment of aqueous extract,the contents of cAMP and cGMP were increased,the ratio of cAMP/cGMP was decreased.Meanwbile,the content of testosterone and mice organ index were also decreased while the content of es-

目的 通过正交设计优选锁阳中儿茶素、没食子酸、原儿茶酸的最佳提取工艺。方法 以提取液中儿茶素、没食子酸、原儿茶酸含量为指标,采用L9（3^4）正交设计安排试验,考察溶剂种类、溶剂用量、提取时间的影响。结果 最佳提取工艺条件为,药材过40目筛,加5倍量水,超声提取30 min。结论 优选的提取工艺可提高锁阳中儿茶素、没食子酸、原儿茶酸的提取得率,工艺较简单、适用。

Objective To optimize the best extraction process of catechin,gallic acid and protocatechuic acid in Cynomorium songar-icum by the orthogonal test. Methods With the contents of catechin,gallic acid and protocatechuic acid in the extracting solution as the observing indexes,the L9 (34 ) orthogonal design was adopted to arrange the experiments for investigating the influences of the solvent type,solvent amount and extracting time on the exacting process. Results The optimum conditions of extraction process were as follows:the medicinal materials were sifted by 40 mesh,adding 5 times amount of water into the materials and ultrasonic extracting for 30 min. Conclusion The optimized extraction process can improve the yield rate of catechin,gallic acid and protocatechuic acid in Cynomorium songaricum,the technology is simple and practical.

研究锁阳多糖(CSP)对衰老模型小鼠免疫功能的影响。方法:昆明小鼠腹腔注射D-半乳糖500mg.kg-1.d-1制作衰老模型,低、中、高剂量CSP治疗,连续56d后,测定脾脏、胸腺脏器指数,应用MTT法测定骨髓细胞增殖数,通过体内淋巴细胞转化率和腹腔巨噬细胞吞噬率观察非特异性免疫功能。应用Elisa法测定睾丸组织端粒酶的活性。结果:高、中、低剂量CSP均能显著提高衰老小鼠骨髓细胞增殖数(P0.01),高剂量CSP能够明显提高衰老小鼠体内淋巴细胞转化率(P0.01)和腹腔巨噬细胞吞噬率(P0.05)。中、高剂量CSP组小鼠睾丸组织端粒酶阳性率明显增加。结论:较高剂量的CSP具有提高衰老小鼠免疫功能,增强雄性衰老小鼠生殖细胞端粒酶活性的作用。

OBJECTIVE The effect of Cynomorium Songaricum polysaccharide (CSP) on immune function was studied on the aged mice for CSP''s applying for the clinical uses. METHODS Kunming mice were intraperitoneal injected with D-galac-rose to make the aging models, and different dosages of CSP were given by gavage for 56 days. Spleen coefficient and thymus coefficient were determined. MTT method was used to detect the proliferated number of the bone marrow cells. Lymphocyte transformation rate and peritoneal macrophage phagocytic rate in vivo were tested. The activity of telomerase in the testicles of the mile mice were detected by Elisa method. RESULTS Low, middle and high dosages of CSP could cause a significant in-crease of the proliferated number of the bone marrow cells in the aged mice (P<0.01 ). High dosages of CSP could significant-ly increase the lymphocyte transformation rate (P<0. 01) and the peritoneal macrophage phagocytic rate (P<0.05). High and middle dosages of CSP could s