目的：探讨苦参碱与氧化苦参碱对人肝癌SMMC-7721细胞凋亡的影响。方法采用MTT法检测SMMC-7721细胞增殖，并采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶双染法检测SMMC-7721细胞凋亡。结果苦参碱与氧化苦参碱浓度为0.50～2.00 mg/ml时，细胞增殖抑制率均逐渐上升，呈药物浓度和作用时间的依赖性；同样浓度下（1.00 mg/ml），苦参碱增殖抑制作用强于氧化苦参碱[24 h、48 h和72 h时苦参碱组分别为（42.39±0.04）%、（51.69±0.03）%、（78.98±0.05）%，氧化苦参碱组分别为（21.36±0.02）%、（36.16±0.02）%、（61.24±0.13）%；P均＜0.05]。苦参碱与氧化苦参碱浓度为0.25、0.50、1.00 mg/ml时SMMC-7721细胞凋亡率显著增高；同样时间点（48 h），苦参碱对细胞凋亡的诱导强于氧化苦参碱[0.25、0.50、1.00 mg/ml时，苦参碱组凋亡率分别为（4.08±0.20）%、（4.32±0.19）%、（9.93±0.18）%，氧化苦参碱组分别为（2.20±0.18）%、（3.08±0.26）%、（9.01±0.20）%；P均＜0.05]。结论苦参碱和氧化苦参碱可抑制人肝癌SMMC-7721细胞增殖，促进其凋亡。

Objective To explore the effects of matrine and oxymatrine on apoptosis in human hepatocarcinoma SMMC-7721 cells.Methods The MTT assay and double staining of annexin V-fluorescein isothiocyanate(annexin V-FITC)/propidium iodide(propidium iodide, PI)were used to detect proliferation and apoptosis of SMMC-7721 cells, respectively.Results When the concentrations of matrine and oxymatrine were 0.50 mg/ml, 1.00 mg/ml and 2.00 mg/ml, the proliferation inhibition rates in SMMC-7721 cells was gradually increased in a dose- and time-dependent manner,and the inhibition of matrine on proliferation were greater than that of oxymatrine in the same concentration(1.00 mg/ml)(24 h:42.39%±0.04%vs. 21.36%±0.02%;48 h: 51.69%±0.03%vs. 36.16%±0.02%;72 h: 78.98%±0.05%vs. 61.24%±0.13%;allP<0.05). When the concentrations of matrine and oxymatrine were 0.25 mg/ml, 0.50 mg/ml and 1.00 mg/ml, the apoptosis rates of SMMC-7721 cells were significantly increased;and induction of matrine in apoptosis

中药苦参的主要成分为苦参生物碱，通过整理和分析文献资料，对苦参生物碱药理作用及临床应用方面进行综述。苦参生物碱有抗心律失常、抗肿瘤、免疫调节、抗生育、平喘等多种药理作用，在银屑病、湿疹、病毒性肝炎、肺结核、青光眼等方面有重要应用。

The main ingredients of traditional Chinese medicine forSophora alkaloids and its pharmacological effects and clinical applications were reviewed through data collation and analysis.Sophora alkaloids have anti-arrhythmic, anti-tumor, immunomodulatory, anti-fertility, asthma and other pharmacological effects.Sophora alkaloids are widely used in the treatment of psoriasis, eczema, viral hepatitis, tuberculosis, glaucoma, etc. .

目的对多叶越南槐中苦参碱与氧化苦参碱进行分离及含量测定。方法采用溶剂提取、萃取、硅胶色谱柱分离纯化,利用色谱技术进行化学结构鉴定;高效液相色谱(HPLC)法色谱条件为:Kromasil 100-5 NH2(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-磷酸二氢钠(0.05 mol/L)-磷酸水溶液(3.0%)-乙醇(80∶5∶9.5∶5.5);流速为1.0 ml/min;检测波长为210 nm;柱温为30℃。结果从多叶越南槐中分离得到两生物碱类化合物,经鉴定为苦参碱与氧化苦参碱;苦参碱在0.0164~0.1476μg范围与其峰面积呈良好的线性关系,氧化苦参碱在0.2872~2.5848μg范围与其峰面积呈良好的线性关系。苦参碱和氧化苦参碱的加样回收率分别为100.2%(RSD=0.76%)、99.8%(RSD=1.19%)。结论苦参碱与氧化苦参碱均为首次从多叶越南槐中分离得到;所建立的HPLC简便、准确、重现性好,结果准确可靠,可用于多叶越南槐中苦参碱与氧化苦参碱的含量测定。

Objective To study the isolation and assaying of matrine and oxymatrine in Sophora tonkinensis Gagnep.var.Polyphylla.Methods The chemical constituents were extracted by solvent and then isolated by silica gelchromatographycolumn .The chromatographic technique was used to identify its chemical structures .The chromatographic conditions of assaying by high performance liquid chromatography ( HPLC ) as follows:Kromasil 100-5 NH2 column (250 mm ×4.6 mm,5 μm) with acetonitrile-monometallic sodium orthophosphate (0.05 mol/L)-phosphoric acid solution(3.0%)-ethylalcohol (80 ∶5 ∶9.5 ∶5.5) as mobile phase,the flow rate was 1.0 ml/min,the UV detection wave length was 210 nm,and the column temperature was 30℃.Results The two alkaloids compounds attained from the separation of Sophora tonkinensis Gagnep.var.Polyphylla were identified as matrine and oxymatrine .The matrine formed linear relation with its peak area when it was in the range of 0.0164 -0.1476 μg.The oxymatrine formed l

本次研究通过控制注射用苦参总碱(冻干)中苦参总碱的质量指标和药效学的比较研究确定了最佳的提取工艺为工艺Ⅲ,并对质量标准进行合理的改进和提高,以期获得符合临床制剂质量标准要求的苦参总碱,从而使注射用苦参总碱(冻干)更加安全、有效和质量可控。

We optimized the extraction process of alkaloids of Sophora flavescens Ait.for its injection(freeze-dried) ,and we de-termined the third process was best.We improved the quality standard.The aim of this research is to assure alkaloids of Sophora flave-scens for its injection(freeze-dried) was more securety, effective and quality control.

目的：优选山豆根药材的提取工艺。方法：以提取物中苦参碱的含量为研究指标，采用高效液相色谱法测定其含量；比较水提醇沉法、60℃酸水浸泡醇沉，pH8-9氨水提取醇沉法、60%乙醇加热回流法等不同工艺的差异。结果：60%乙醇加热回流法苦参碱含量较高，苦参碱含量在6.24μg～399.04μg范围里呈较好的线性关系；苦参碱的回收率为100.08%。结论：60%乙醇加热回流法，提取山豆根药材的提取物中苦参碱含量较高，苦参碱转移率最高，提取方法合理、操作简单，适合用于大生产提取工艺。

Objective: To optimize the extraction technology for the Sophorae Tonkinensis Radix Et Rhizoma. Methods: Taking the content of the Matrine within the extracts as a study index, we use HPLC to determine its content. Then we compare differences among Water extraction - Alcohol precipitation,60℃Acid water soaking- Alcohol precipitation,pH8-9 Ammonia water extraction Alcohol precipitation and 60% Alcohol Heating reflux method.Result: The content of the Matrine from 60% Alcohol Heating reflux method is the higher.In the range of 6.24μg~399.04μg,the content of the Matrine shows a good linear relationship and the recovery is 100.08%.Conclusion: Using the 60% alcohol Heating reflux method, the content of the Matrine from the Sophorae Tonkinensis Radix Et RhizomaExtracts is the higher, with a highest metastasis rate. The extracting method is reasonable, simple and suitable for industrialized production.