目的：分析海南产温莪术挥发油的化学成分，为其资源利用和质量评价提供依据。方法采用水蒸气蒸馏法提取挥发油，以海南产的温莪术挥发油和浙江产的温莪术挥发油为研究对象，采用GC-MS法、HPLC色谱法，根据《中国药典》公布的莪术油的质量标准分析其挥发油的化学成分。结果海南产温莪术挥发油与浙江产温莪术挥发油的成分基本一致，两种挥发油的特征图谱与《中国药典》2010年版公布的莪术油的特征图谱比对，相似度均达到95%以上。结论不同产地的温莪术挥发油成分有一定的差异，但主要成分基本相同，为海南产温莪术的开发利用提供了科学依据。

Objective To analyze the essential oil compositions of Curcuma wenyujin Rhizoma from Hainan Province in order to provide identifiable ground for the use of resources and quality evaluation. Methods Essential oil of Curcuma wenyujin Rhizoma from Hainan Province and from Zhejiang Province was selected as the research ob-jects. These essential oil were extracted by steam distillation. They were analyzed and determined by gas chromatogra-phy-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC), compared with the quality standards of"China pharmacopoeia". Results Essential oil compositions of Curcuma wenyujin Rhizoma from Hain-an Province and Zhejiang Province were consistent. Similarity of characteristic chromatogram of two essential oils reached more than 95%compared with Chinese pharmacopoeia characteristic chromatogram (2010) of Curcuma we-nyujin Rhizoma. Conclusion Essential oil compositions of different origin are similar. The study provided scientific

目的：探讨能够将莪术有效成分充分煎出的最佳饮片类型。方法：将莪术饮片按厚度分成4mm厚片组、2～3mm细丝组和2mm薄片组，将每组莪术都平均分成三等份，将第一份浸泡0.5 h，将第二份侵泡1 h，将第三份侵泡1.5 h，以水煎法测定这三种莪术饮片的挥发油含量及浸出物含量。结果：2～3mm细丝组莪术饮片的挥发油含量和浸出物含量均明显高于4mm厚片组和2mm薄片组。结论：2～3mm的细丝莪术饮片是最适宜煎煮莪术饮片类型。

Objective: To explore the most suitable water extract of Curcuma Pieces type. METHODS: Curcuma 4mm thick sheet , the sheet of 2mm , 2 ~ 3mm thick filaments of the four groups of the three equal parts , the soaking time was set to 0.5 h, 1 h, 1.5 hours to cook four different types of cooking determination Pieces Curcuma extract content of volatile oil and different immersion time . Results: Volatile oil and extract content of 2 ~ 3mm thick filaments is the highest . Conclusion: Curcuma 2 ~ 3mm thick filaments, is best suited to the type of water extraction Pieces .

目的：对广西莪术叶中挥发油成分进行系统的分析，并与其传统的用药部位块根和根茎的挥发油成分对比，寻找广西莪术叶作为代替性药材的可能性，尽可能的扩大广西莪术的药用部位，提高广西莪术整株植物的经济效益。方法：采用水蒸气蒸馏法提取挥发油，运用GC/MS方法比较叶与根茎，块根的化学成分及其各成分的相对百分含量。结果：从广西莪术叶中分离得到38个色谱峰，鉴定出26种成分。结论：首次从广西莪术叶中鉴定出26种挥发性成分，叶中所含成分与根茎、块根的差别较大，但也含有部分抗肿瘤成分。

Objective:To analyze the volatile oil in leaves of Cureuma Wangsiensis. S. G. Lee et C. F. Liang and compare with that in the traditional medicinal parts ( rhizomes and tuberous roots) to look for the possibility of the leaves as alternative medicinal part, expand the medicinal parts of the Chinese medicine as much as possible and improve the economic benefits of the whole plant. Methods:The volatile oil was extracted by steam distillation method, and the content was determined by gas chromatography-mass spectrometry ( GC/MS) . The chemical composition and content of the volatile oil in leaves, rhizomes and tuberous roots were com-pared. Results:Totally 38 chromatographic peaks were isolated and 26 constituents were identified from the volatile oil in leaves. Con-clusion:Totally 26 volatile constituents are identified from the volatile oil in leaves of Cureuma Wangsiensis. S. G. Lee et C. F. Liang for the first time. Except for some anti-tumor constituents, the other componen

为合理开发使用莪术叶的挥发油成分提供科学依据。方法:用水蒸气蒸馏法提取3种莪术根茎和叶子中的挥发油,进行GC-MS分离测定,利用Nist98标准质谱图库及相关标准品进行对照,鉴定其结构,并测定各成分的相对百分含量。结果:莪术二酮、吉马酮等抗肿瘤活性成分在3种莪术叶子中的含量均较高,甚至远远高于在其根茎中的含量。结论:3种莪术不同部位挥发油成分差别不大,但各成分的相对百分比含量差异明显,可对其叶子进行回收利用。

Objective: To provide the scientific basis for the reasonable development and use of the leaves of Rhizoma Curcumae. Methods: To extract the volatile oil from roots and leaves of three species Rhizoma Curcumae by steam distillation and isolate these products with the method of gas chromatography-mass spectrum. Taking Nist98 standard mass spectrometer database and contrast as control, the structure of the mass spectrogram was identified, and the relative contents of the components were determined with area normalization method. Results: The anti-tumor active constituents such as curdione and germacrone in the leaves had the greater content than in its rhizome. Conclusion: The varieties of volatile components in different parts of three species of Rhizoma Curcumae are similar, but their contents are obviously various. We can make the leaves of Rhizoma Curcumae for recycling use.

目的观察莪术对正常和血瘀证孕大鼠血液流变学的影响。方法采用皮下注射盐酸肾上腺素与冷刺激的方法制备血瘀证模型,造模后将正常组随机分为空白对照组和高、中、低剂量莪术组,将血瘀证模型组随机分为模型对照组和高、中、低剂量莪术组。受孕后第6～19d给予不同剂量的莪术水煎液。检测受孕雌鼠血液流变学指标。结果莪术高、中、低剂量组均能降低正常组与血瘀证模型组在不同切变率下全血黏度。与正常对照组相比较,正常+莪术高剂量组在不同切变率下全血黏度有显著性差异(P0.05),而在中切变率下,各剂量血瘀+莪术组的全血黏度与正常对照组相比较均有显著性差异(P0.05)。结论莪术+血瘀证模型组能使全血黏度降至正常孕大鼠水平,证明了中医"有故无殒,亦无殒"的中药辨证减毒理论。

Objective This study aims at evaluating the effect of curcumae rhizoma on hemorheological parameters between normal and blood -stasis pregnant rats .Methods A model of blood -stasis in rats was established by injecting adrenalin and being immersed in ice water .Normal Wistar female rats were randomly divided into blank control group and low , medium and large dose curcumae rhizoma groups , blood-stasis model rats were divided into model control group , low, medium and large dose curcumae rhizoma groups .Curcumae Rhizoma was orally administered to pregnant rats from gestational day ( GD) 6 through 19 at different doses .The hemorheological parameters were used to monitor the pregnant rats .Results Curcumae rhizoma could significantly reduce the hemorheological indexes in whole blood viscosity .Compared with normal group , whole blood viscosity at high , middle and low shear rate were decreased in rats of normal group treated with Curcumae Rhizoma(P<0.05), and We only could find blood vi