介绍了国内外1,4-丁二醇主要生产工艺和供需现状,结合我国1,4-丁二醇装置建设情况对市场前景进行了分析,认为未来我国1,4-丁二醇市场竞争将更加激烈并提出了相关建议。

The article introduces major technologies for producing 1,4-butanediol (BDO) and the market situaiton both in China and the world. Combining with the analysis of BDO plants construciton situaiton in China, it points out that, the future of China’s BDO market compeititon will become more intense and puts forward relevant suggesitons.

以醋酸正丁酯为内标物,采用毛细管气相色谱内标法测定1,4-丁二醇及其酯化产物的含量。结果表明：1,4-丁二醇、1,4-丁二醇单醋酸酯、1,4-丁二醇双醋酸酯的相对校正因子曲线分别在0.7090~41.30mg·mL^-1、0.5980~41.82mg·mL^-1、0.5429~39.10mg·mL^-1范围内呈现良好的线性关系（R2≥0.9994）,平均加标回收率为94.53%~103.20%,RSD≤4.72%。该方法操作简便、精密度高、重现性好、结果稳定可靠,可用于1,4-丁二醇及其酯化产物的含量测定。

With n-butyl acetate as internal standard,the gas chromatography has been developed for the de-termination of 1,4-butanediol(0.7090~41.30 mg·mL-1 ),1,4-butanediol monoacetate(0.5980~41.82 mg· mL-1 )and 1,4-diacetoxybutane(0.5429~39.10 mg·mL-1 ).All linearity relative coefficients are higher than 0.9994 within their respective ranges.And the average recoveries are from 94.53% to 103.20% with the RSD≤4.72%.This method is simple,accurate,reproducible,stable and reliable,which can be applied to the quanti-tative determination of 1,4-butanediol and its esterification products.

研究了以葡萄糖为原料,在催化剂的作用下,通过加氢裂解得到含有1,2-丙二醇、乙二醇、2,3-丁二醇、1,2-丁二醇、1,4-丁二醇（以下简称二元醇）、山梨醇、丙三醇、甲酸盐、乙酸盐、乳酸盐以及其他未知物（以下简称重组份）的反应液[1],优化了葡萄糖制备生物化工醇的氢化裂解工艺,通过单因素和正交试验,获取最佳氢化裂解工艺条件为：反应压力11MPa,反应温度200℃,pH=12,催化剂用量20％,转速300 r/min,时间3h.在此条件下,葡萄糖转化为二元醇的收率为58.6％.

Glucose as a raw material, under the action of the catalyst, 1,2-propanediol, ethylene glycol, 2,3-bu-tanediol, 1,2-butanediol, 1,4-D diol (hereinafter referred to as diol), sorbitol, glycerol, formate, acetate, lactate, and other unknown compounds ( hereinafter referred to as the heavy component) of the reaction solution were obtained by hydrocracking, the preparation process of polyol glucose by hydrocracking was optimized by single factor and orthogonal experiments, and the best hydrocracking process conditions were reaction pressure 11 MPa, reaction temperature 200 ℃, pH=12, 20% of the amount of catalyst, speed 300 r/min, and time 3 h. Under the conditions, the yield of conversion of glucose to diol was 58.6%.

以氨基磺酸为催化剂,对以正丁醛和1,2-丙二醇为原料合成丁醛1,2-丙二醇缩醛进行了研究。较系统考察了醛醇摩尔比、催化剂的用量、反应时间、带水剂诸因素对缩醛反应的影响。实验结果表明,丁醛为0.2mol,n（丁醛）∶n（1,2-丙二醇）=1∶1.5,氨基磺酸1g,带水剂环己烷15mL,反应时间为60min时,产品的收率可达56.14%。

Butyraldehyde 1,2-propanediol acetal was synthesized from n-butyl aldehyde and 1,2-propylene glycol using aminosul-fonic acid as catalyst. The effects of aldehyde alcohol morlar ratio,the amount of aminosulfonic acid,reaction time and water carrying on product yield were studied. The optimal reaction conditions were identified as followed:the amount of butly aldehyde was 0.20mol, n(aldehyde):n(1,2-propanediol)=1∶1.5;the dosage of catalyst was 1g,the volume of cyclohexane was 15mL,the reaction time was 60min,and the yield of butyraldehyde 1,2-propanediol acetal could reach 56.14%.

目前2,3-丁二醇生产菌株大部分为致病菌,对人类健康和环境具有一定威胁。从牛奶样品中分离到1株产2,3-丁二醇的芽孢杆菌127-7,分析其16S rRNA基因序列,确定该菌株为地衣芽孢杆菌(Bacillus licheniformis)。进一步对菌株127-7进行紫外诱变,筛选耐受高浓度葡萄糖和高产乙偶姻的菌株。摇瓶发酵结果显示,突变株BL41的2,3-丁二醇产量较出发菌株127-7提高了41.1%。对发酵副产物分析发现,不控制发酵液pH可以显著降低乳酸产量,2,3-丁二醇产量在72 h达到81.4 g/L。进一步调整补糖策略,维持最低残糖浓度为30 g/L,菌株BL41产2,3-丁二醇83.4 g/L,最高产率为1.9 g/L·h,发酵时间缩短至46 h。结果表明,地衣芽胞杆菌BL41可以作为候选菌株,用于工业规模2,3-丁二醇的生产。

Currently, most isolated 2,3-butanediol producing strains are pathogens and they cause certain risks to public health and environments. In this work, Bacillus sp. 127-7 was isolated for 2,3-butanediol(BD)production from raw milk samples. It was identified as Bacillus licheniformis by analyzing its 16S rRNA gene sequence. By UV-mutagenesis, mutants that could endure high glucose concentrations and produce relatively high amounts of acetoin were isolated for further analysis. In shake-flask fermentations, 2,3-BD production was increased by 41.1%in mutant strain BL41 compared with the parent strain 127-7. Additionally, BL41 yielded much less amounts of lactate acid in the cultures without acidity control and 2,3-BD reached 81.4 g/L in the fed-batch fermentation. Moreover, the minimal residual glucose concentration was incre ased to 30 g/L in the culture, the fermentation time was significantly reduced to 46 h with 83.4 g/L 2,3-BD. The highest productivity is up to 1.9 g/L·h. Th