为了优化东亚飞蝗消化系统双向电泳技术,建立东亚飞蝗消化系统蛋白表达图谱,探讨雌、雄东亚飞蝗消化系统蛋白组分的差异,利用pH值为3~10和pH值为4~7的胶条分别对雌、雄东亚飞蝗消化系统进行双向电泳分析,进行蛋白组学分析.研究结果发现:雌性东亚飞蝗消化系统蛋白种类多于雄性,雌性东亚飞蝗消化系统特异蛋白中偏酸性蛋白数量与偏碱性蛋白相当,而雄性东亚飞蝗消化系统特异蛋白中酸性蛋白多于碱性蛋白.在雌、雄东亚飞蝗消化系统相匹配蛋白中,含量差异达3倍以上的蛋白约占总匹配蛋白的50%.可见,雌、雄东亚飞蝗消化系统蛋白组分存在差异.

Two-dimensional electrophoresis system was optimized and carried out to establish protein expression map of alimentary system from Locusta migratoria manilensis (Meyen) and then the proteomics difference was in-vestigateed between female Locusta migratoria manilensis (Meyen) and male ones. Two-dimensional electrophore-sis was carried in pH 4~7 and pH 3~10 in order to obtain the best condition and to analyze the proteomics differ-ence between female Locusta migratoria manilensis (Meyen) and male ones. It was found that there were more kinds of protein in alimentary system from female Locusta migratoria manilensis than that in male ones. Among the special protein from female Locusta migratoria manilensis, the quantity of acid protein has the similar level with that of alkaline protein while quantity of acid protein is larger than that of alkaline protein in male Locusta migratoria manilensis. In addition, the quantity of the matched protein spots whose abundance in female or ma

采用活体生物试验方法进行活性跟踪，选取瑞香狼毒对东亚飞蝗毒杀活性最高的石油醚萃取部分，测定瑞香狼毒活性成分对东亚飞蝗的生物活性及其中肠消化道的组织病理学影响．结果表明：瑞香狼毒活性成分对东亚飞蝗具有拒食、触杀、胃毒活性及生长发育抑制作用，其中拒食活性最为显著，24 h、48 h拒食中浓度（A FC50）分别为317．63、113．20 mg/L ；96 h触杀致死中浓度（LC50）、胃毒致死中浓度（LC50）分别为548．21 mg/L、2486．19 mg/L ；生长发育抑制作用也十分明显，48 h生长发育抑制中浓度（EC50）为39．75 mg/L ．东亚飞蝗中肠组织病理学变化研究结果表明，瑞香狼毒萃取物可破坏东亚飞蝗中肠的正常组织结构，引起其中肠柱状上皮细胞脱落坏死．

The activity of ligarine extracts from Stellera chamaej asme L .against Locusta migratoria manilensis(Meyen) was tested and compared by bioassay methods . The biological activity and histopathological effects of the active ingredients from Stellera chamaej asme L .on Locusta migratoria manilensis(Meyen) and mid-gut cells were determined .The results showed that the active ingredients from Stellera chamaej asme L .had anti-feeding activity ,contacting activity ,stomach toxicity activity and inhibition of growth and development on Locusta migratoria manilensis(Meyen) .The AFC50of complete anti-feeding activity were 317 .63mg/L ,113 .20mg/L in 24 hour and 48 hour ;The LC50 of contacting activity were 548 .21 mg/L and the LC50 of stomach toxicity activity were 2486 .19 mg/L in 96 hour for ligarine extracts from Stellera chamaej asme L .,active ingredients also had strong effect on growth and development of Locusta migratoria manilensis(Meyen) .The EC50of growth inhibition rate was 39 .75 m

温度是影响蝗虫滞育的重要环境因子,为明确有效积温对东亚飞蝗卵滞育的影响,本文设置6个有效积温梯度处理饲养2代飞蝗,结果表明544.3、662.2、678.8、760.2、840.0、843.5日度处理下蝗卵滞育率为73.1%、58.6%、48.6%、12.1%、0、0;越冬蝗卵部分滞育,滞育率(D)与越冬前卵期有效积温(Y)负相关,D=-0.719Y+82.75,R2=0.774,Y≥115.1日度时蝗卵全部孵化。进一步分析越冬前不同温度积累有效积温对蝗卵滞育影响,结果表明27℃以上有效积温(E)对滞育率(D)影响最显著,呈线性负相关,D=-0.058E+0.732,R2=0.967。越冬蝗卵经历负积温(X)越小,滞育率(D)越低,呈线性负相关:D=-0.155ln(|X|)+1.136,R2=0.970。本文明确了不同时期越冬蝗卵滞育率与有效积温关系,为飞蝗发生期、发生量预测提供理论依据。

We reared oriental migratory locusts, Locusta migratoria manilensis (Meyen) in the laboratory from egg stage until egg-laying female adults, at six different degree-days (544.3, 662.2, 678.8, 760.2, 840.0 and 843.5 heat units), to reveal the influence of degree-days (DD) on diapause rate of overwintering locust eggs. Results showed that the diapause rate of eggs at the six treatments was 73.1%, 58.6%, 48.6%, 12.1%, 0 and 0, respectively. This indicated there were only parts of overwintering locust eggs could enter diapause during winter. The relationship between diapause rate of eggs (D) and DD of pre-overwintering period (Y) was D=?0.719Y+82.75, R2=0.774. When Y≥115.1 DD, all overwintering locust eggs would hatch. We further studied the sensitive temperature on diapause rate of eggs at the six treatments, results showed that DD based on temperature over than 27 ℃ (E) had the significant effect on diapause rate of eggs (D), the relationship was D=?0.058E+0.732, R2=0.967. Th