研究姜黄素对人永生化表皮HaCaT细胞凋亡过程中相关基因表达的影响。方法:通过免疫细胞化学法和免疫印迹检测姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响。结果:免疫细胞化学染色结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达减弱,促凋亡蛋白P53、Fas、Bax表达增强。Western blot检测结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达下调,促凋亡蛋白P53、Fas、Bax表达上调。结论:姜黄素诱导HaCaT细胞凋亡过程中伴随着抑凋亡基因bcl-2表达的抑制和促凋亡基因p53、Bax和Fas基因的激活。由此证明姜黄素通过多种凋亡相关基因的调控,激活凋亡信号传导途径,诱使细胞凋亡。

To explore the effects of curcumin on apoptosis-related gene expression in HaCaT cells. METHODS:Immunocytochemistry and Western blot were used to detect the effects of curcumin on apoptosis-related protein expression. RESULTS:Immunocytochemistry staining results showed that the expression levels of P53,Bax,Fas were upregulated significatntly while the products of Bcl-2 proteins were downregulated significantly in cells treated with 7.5 μg/mL curcumin. Western blot analysis confirmed the same results. CONCLUSION:Curcumin induced apoptosis of HaCaT cells by inhibiting expression of Bcl-2 gene and promoting the apoptosis genes p53,Bax and Fas activation. Curcumin could induce apoptosis by regulation of a variety of apoptosis-related genes to activate the apoptosis signaling pathway.

目的：探讨桂皮醛对人胃癌BGC-823细胞增殖、凋亡及其相关分子机制。方法：采用MTT法检测桂皮醛对人胃癌BGC-823细胞增殖的影响；采用流式细胞仪检测桂皮醛诱导的凋亡，Hoechst 33342染色法观察凋亡形态的变化；RT-PCR法检测桂皮醛对人胃癌BGC-823细胞相关凋亡基因的影响；Western Bolt方法检测桂皮醛对人胃癌BGC-823细胞相关凋亡蛋白的影响。结果：与对照组相比，桂皮醛有抑制人胃癌BGC-823细胞增殖的作用（P<0.01）；桂皮醛能诱导凋亡的产生；桂皮醛能下调凋亡相关基因Bcl-2、Bcl-xL、Survivin，上调凋亡相关基因Bax、Bak；桂皮醛能下调凋亡相关蛋白Bcl-2、Procaspase-3，上调凋亡相关蛋白Bax。结论：桂皮醛对人胃癌细胞BGC-823的增殖具有抑制作用并能诱导凋亡，可能与内源性凋亡途径的激活有关。

This article was aimed to investigate the cell proliferation , cell apoptosis and its related molecular mechanisms of the human gastric carcinoma cell line BGC-823 in v itro after treatment with cinnamaldehyde . The MTT Assay demonstrated the inhibitory effect of cinnamaldehyde . And the Flow Cytometry was used to determine its induction of cell apoptosis. The Hoechst 33342 was used to observe morphological changes during apoptosis . Moreover , quantitative real time PCR and western blot analysis were used to detect the effect of cinnamaldehyde on human gastric carcinoma cell line BGC-823 . The results showed that compared with the control group , cinnamaldehyde had inhibitory effect on human gastric carcinoma cell line BGC-823 ( P <0 . 01 ) . It showed that cinnamaldehyde induced apoptosis through the downregulation of Bcl-2 , Bcl-xL and Survivin expression , upregulation of Bax and Bak expression , downregulation of Bcl-2 and Procaspase-3 , and upregulation of BAX . It was concluded

目的利用流式细胞技术检测耐多药结核分枝杆菌(Multidrug resistant mycobacterium tuberculosis,MDRTB)临床分离株和结核分枝杆菌一、二线药物敏感株分别感染U937细胞后的凋亡率及其时相性变化。方法用两组细菌分别感染U937细胞,在感染后2、4、8及12 h用流式细胞技术检测各组感染U937细胞的凋亡率,并分析其时相性变化。结果结核分枝杆菌MDR株和敏感株均能诱导U937细胞凋亡,两组细胞凋亡率比较差异有统计学意义(P0.01),MDR株诱导U937细胞凋亡率显著低于敏感株诱导的凋亡率,且在诱导后2、4、8及12 h各时间点的凋亡率都显著低于敏感株组,诱导早期凋亡率升高显著,特别是诱导后2 h凋亡率升高最显著,4 h以后凋亡率升高逐渐变慢。结论结核分枝杆菌临床分离株感染U937细胞后能快速诱导其凋亡,凋亡率与诱导菌株的耐药表型相关,敏感株诱导的凋亡率显著高于MDR株,诱导早期凋亡率升高最显著,4 h以后凋亡率升高减慢。

Objective To compare the apoptosis rate of macrophages U 937 infected with clinical isolates of MDR-TB and susceptible Mycobacterium tuberculosis by using a flow cytometry .Methods Macrophages U937 were respectively infected with MDR-TB and susceptible clinical isolates , then the apoptosis rate of macrophages U 937 were detected by the flow cy-tometry after infection in 2, 4, 8 and 12 hs and the phase changes of apoptosis rate of infected macrophages U 937 were ana-lyzed.Results U937 apoptosis can be induced both by the MDR-TB and the susceptible isolates , but the differences are significant in the apoptosis rates in between two types of isolates (P<0.01).The apoptosis rates of U937 infected with MDR-TB are significantly lower than that with susceptible isolates , and all of the apoptosis rates of U 937 after infection in 2, 4, 8 and 12 hs with MDR-TB are significantly lower than that with susceptible isolates .The apoptosis rates of U937 in-crease markedly in early stage of infectio

细胞凋亡存在于多细胞生物的整个生命过程当中,可及时清除机体内多余和受损伤的细胞,维持组织器官的稳定性。真核细胞主要通过死亡受体介导的外部凋亡途径、内部线粒体途径、B粒酶介导的细胞凋亡途径以及近几年开始关注的内质网应激途径介导细胞凋亡的发生。而参与这些细胞凋亡过程的主要有4类蛋白分子,即凋亡蛋白酶(caspases)、衔接蛋白(adapter proteins)、Bcl-2和凋亡抑制蛋白(IAPs)。细胞凋亡对卵巢储备及一些妇科疾病如子宫内膜异位症、卵巢癌等的发生发展产生影响。

Cell apoptosis exists in the whole life of multicellular organisms ,which is capable to maintain homeostasis of tissues and organs through removing unwanted or damaged cells. Eukaryotic cells mediate apoptosis through four main pathways:the death receptor-mediated extrinsic pathway,the intrinsic mitochondrial pathway,granzyme B-mediated pathway and endoplasmic reticulum stress-mediated pathway. There are four important protein molecules involved in apoptotic pathways:caspases (the proteases which execute cell death),adapter proteins (caspase activators),Bcl-2 family proteins and Inhibitor of apoptosis proteins (IAPs). Cell apoptosis plays an important role in establishment of ovarian reserve and abnormal apoptosis inducing some diseases,such as endometriosis,ovarian cancer,et al. In this review we will discuss the apoptotic pathways and their mechanisms mentioned above.

目的探讨小鼠肾脏发育过程中的细胞凋亡规律及相关形态学特征。方法在树脂切片上,应用光镜技术和原位末端标记法(TUNEL)分别观察不同胚龄及生后不同日龄小鼠肾脏细胞凋亡。结果胚龄12 d的小鼠肾脏输尿管芽中即存在细胞凋亡现象,皮质凋亡细胞多出现在生肾区S小体之间和肾小体内,皮质中的肾小体凋亡高峰期在胚龄14~18 d。而肾小管、髓放线及髓质凋亡细胞出现在肾小管上皮内,三者的凋亡高峰期均出现在生后7 d左右。观察发现皮质和髓质凋亡细胞被邻近细胞吞噬,或部分脱落到肾小管腔内。结论小鼠肾脏发育过程中存在细胞凋亡,凋亡现象在后肾形成时即存在;皮质中细胞凋亡与生肾区的出现和肾小体发育完善有关,髓质中的细胞凋亡则与髓质中肾小管和集合管的改建、完善有关。

Objective To investigate the cell apoptosis in kidney development in mice and related morphological characteristics.Methods In the resin sections,light microscopy and in situ end labeling(TUNEL) were applied to observing embryos and postnatal apoptosis of renal cells in mice of different ages .Results The cell apoptosis was found in embryonic kidney ureteric bud of 12-day-old mice, the cortical apoptosis cells mostly appeared between S bodies and renal corpuscle body in nephrogenic zone ,the apoptosis peak time of renal corpuscle in the cortex was embryonic age of 14-18 days.The renal tubules,medullary rays and medulla apoptotic cells were found in the renal tubular epithelium,their apoptosis peak appeared on the 7th day after birth.The observation on cortex and medulla apoptotic cells showed that the cells were phagocytized by neighboring cells,or partially exfoliated into the tubular lumen.Conclusion The apoptosis exists during the development of kidney in mice,which does exist in th