原子吸收分光光度法具有灵敏度高、精密度好、准确度高、测量元素多等特点，因此在环境监测中得到广泛应用。本文重点阐述了原子吸收光度法最佳测试条件的选择，测量时干扰的种类和消除方法以及近年来原子吸收分光光度法在环境监测中的应用。

atomic absorption spectrophotometric method has the characteristics of high sensitivity,good precision, high accuracy,measuring element and so on,so it has been widely used in environmental monitoring. This paper focuses on the best test condition of atomic absorption spectrophotometry,and the methods to eliminate interference type measurement in recent years and the application of atomic absorption spectrophotometry in environmental monitoring.

用硅钼蓝分光光度法测定制浆黑液中的二氧化硅含量,可替代传统硅含量测定方法。分光光度法操作简单,干扰少,不易引入操作误差,数据准确可靠,适于批量检测。

Silicon dioxide content in black liquor was determined by silicomolybdenum blue spectrophotometry, which can be substituted for traditional method. This method has the advantages of simple operation, less interference, less operation errors, high accuracy, is suitable for batch inspection.

建立长春瑞滨的紫外分光光度测定方法。方法:采用紫外分光光度法,以268nm为检测波长,对长春瑞滨含量进行直接测定。结果:长春瑞滨浓度在1.0~20.0μg/mL范围内线性关系良好。结论:紫外分光光度法简便、准确、灵敏,可用于酒石酸长春瑞滨注射液中长春瑞滨含量的测定。

Objective:To develop a ultraviolet spectrophotometry (UV)method for the determination of Navelbine (NVB). Methods:The content of NVB was directly measured by UV spectrophotometry and 268 nm wavelength was used for the detection. Results:The NVB concentration was linear relationship with absorbance in the range of 1.0~20.0μg/mL.Conclusion:The method is simple,accurate and sensitive,which can be applied to determine the trace amount of NVB in inj ection sample.

基于钼酸铵分光光度法建立一种适用于糖厂工业磷酸含量的检测方法。应用国标方法对钼酸铵分光光度法进行验证，结果为：样品加标回收率在100%～109%之间，精密度实验结果相对标准偏差小于4.0%，浓度在0.02～0.72 mg/L之间线性相关良好，方法合成不确定度为1.8041%，专属性实验表明磷酸杂质不影响方法测量准确性。钼酸铵分光光度法在2个不同糖厂测定85%工业磷酸平均值为84.738%和83.152%，样品加标回收率平均为100.20%和104.09%。钼酸铵分光光度法能适用于糖厂工业磷酸含量的测定。

Based on ammonium molybdate spectrophotometric method, a detection method was established to determine industrial phosphoric acid content. Recovery of sample was between 100%~109%; the relative standard deviation of test results precision was less than 4.0%; the concentration of sample phosphorus had a preferable linear correlation in the range of 0.02~0.72 mg/L, method for synthesis uncertainty was about 1.8041%; specificity experiments showed that phosphoric acid with impurity did not affect the accuracy of measurement. The average to determine two industrial phosphoric acid with 85% concentration were 84.738% and 83.152%, and the average recovery were 100.20% and 104.09%. Ammonium molybdate spectrophotometric method can apply to the determination of phosphate content in sugar mill industry.

溶血分光光度法是溶血空斑形成试验和分光光度法的结合[1-2],溶血空斑形成试验是体外检测B细胞功能的一种方法,基本原理是将经绵羊红细胞(sheep red blood cell,SRBC)免疫小鼠的脾细胞与一定量的SRBC混合,在补体参与下使抗体形成细胞周围那些受到抗体分子致敏的SRBC溶解,形成肉眼可见的溶血空斑.分光光度法是通过测定被测物质在特定波长处或一定波长范围内光的吸光度或发光强度,对该物质进行定性和定量分析的方法,单色光辐射穿过被测物质溶液时,在一定的浓度范围内被该物质吸收的量与该物质的浓度和液层的厚度成正比.溶血分光光度法是通过分光光度计检测抗体形成细胞产生和分泌的抗体溶解SRBC所释放的血红蛋白吸光度(A)值[3-4].

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