目的：分析化学发光法（微粒子酶免法）与酶联免疫吸附实验（ELISA）及胶体金免疫沉析法检测乙肝表面抗原的差异性。方法：利用雅培i2000化学发光分析仪测定病人标本，记录乙肝表面抗原的定量结果，将同样的标本用酶联免疫吸附实验及胶体金检测，记录检测结果，经统计学处理，对三种方法的测定结果进行比较。结果：三种方法测定乙肝表面抗原的结果存在差异，化学发光法的阳性检出率较ELISA法及胶体金免疫沉析法高。结论：对ELISA法及胶体金免疫沉析法检测乙肝表面抗原处于临界状态的标本，应使用化学发光法检测，以提高结果的准确性，更好的为临床提供诊断依据。

Analysis of chemiluminescence (microparticle enzyme immunoassay method) and enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation, analysis of coloidal gold was detected difference between hepatitis B surface antigen.Methods:Abbot i2000 using chemiluminescence analyzer patient specimens. Record the results of the quantitative hepatitis B surface antigen, Wil have the same specimens by enzyme-linked immunosorbent assay and coloidal gold detection,Record test results, Statistical analysis,Determination of the three methods were compared.Results:Three Methods for measuring the results of hepatitis B surface antigen differences,Chemiluminescence than the positive rate of ELISA ,and immunogold method with high precipitation.Conclusion:On ELISA, and immune precipitation of coloidal gold assay of hepatitis B surface antigen in the critical state of the specimens,Chemiluminescence detection should be used to improve the accuracy of the results,the beter the basis for the clinical d

目的探讨2种不同化学发光系统(安图ADC CLIA 400和西门子centaur xp)测定肿瘤标记物结果的可比性。方法在安图化学发光仪ADC CLIA 400和西门子centaur xp化学免疫发光仪中进行50例标本肿瘤标记物的检测,分析比较结果。结果用2种化学发光法对血清标本进行定量分析结果表明2种检测系统的检测结果差异无统计学意义(P0.05),5个项目在2种检测系统之间各相关系数均小于0.836,提示2种方法相关性较好。西门子centaur xp化学免疫发光仪检测肿瘤标记物的准确性,重复性高于安图化学发光仪ADC CLIA 400。结论西门子centaur xp化学免疫发光仪检测肿瘤标记物优于安图化学发光仪ADC CLIA 400。

Objective To study the results comparability of two kinds of different chemiluminescence systems(Antu chemilumi-nescence analyzer ADC CLIA 400 and Siemens centaur xp)in the detection of tumor markers.Methods The tumor markers were detected in 50 specimens by using the Antu chemiluminescence analyzer ADC CLIA 400 and the Siemens centaur xp chemilumines-cence analyzer respectively.Then the results were analyzed and compared.Results The quantitative analysis results on the serum specimens showed that there was no significant difference in the detection results between the two kinds of detection system(P >0.05).The correlation coefficients of 5 test items between the two kinds of detection system were more than 0.836,suggesting a good correlation between the two kinds of method.The accuracy and repeatability of the Siemens centaur xp chemiluminescence ana-lyzer were higher than those of the Antu chemiluminescence analyzer ADC CLIA 400 in detecting the tumor markers.Conclusion The

目的以O,O-二乙基硫代磷酰氯和鲁米诺为原料,合成一种新型的化学发光标记物,用于后续的二乙氧基类有机磷农药多残留化学发光免疫法检测。方法取鲁米诺和O,O-二乙基硫代磷酰氯溶于二氯甲烷中,以三乙胺作为缚酸剂,冰浴下反应8 h后分出有机层,产物经柱层析纯化,即得到化学发光标记物。结果产物经紫外、红外鉴定,实验结果表明反应产物即为所需的化学发光标记物,产物在BPCL上进行发光特性测定,表现出较强的发光能力,最大发光强度达到9000 mV。结论本方法合成的化学发光标记物具有较强的发光能力,能用于后续的二乙氧基类有机磷农药多残留的化学发光免疫检测。

Objective Chemiluminescent markers were synthesized using O,O-diethylthiophosphoryl chloride and luminol for the multi-residue determination of organophosphorus pesticides. Methods Luminol and O, O-diethyl phosphoryl chloride were added to dichloromethane under ice-cooling with triethylamine as acid binding agent. After 8 h, the organic layer was separated and purified by column chromatography. Results The product was the chemiluminescent markers after indentation using UV and IR. It had a strong luminescent ability on the BPCL, and the maximum luminous intensity reached 9000 mV. Conclusion The chemilumi-nescent markers synthesized by this method had a strong luminescence capability, and it could be used in the multi-residue determination of organophosphorus pesticides.

目的：对生物芯片化学发光免疫法在检测人TSH和T4中的价值进行评价分析，为今后的临床研究工作提供可靠的参考依据。方法建立生物芯片化学发光免疫法，采集15例患者血清展开TSH和T4水平检测，并对测定结果进行统计分析。结果所建立的生物芯片化学发光免疫法检测TSH的灵敏度为0.096mIU/L，T4的灵敏度为4.5ng/ml，线性关系良好，TSH的回收率在91%-101%之间，T4的回收率在92%-103%之间，检测结果准确。结论采取生物芯片化学发光免疫法对人体血清中TSH和T4水平进行检测时具有较高的灵敏度、线性关系良好，回收率与准确性高，值得关注并推广。

objective to biochip, chemiluminescence immunoassay in detecting TSH and makes an analysis of the value of T4, provide reliable reference for future clinical research work. Method to establish the biological chip, chemiluminescence immunoassay, col ection of 15 patients with serum TSH and T4 level detection, and the measurement results were analyzed. Results of biochip, chemiluminescence immunoassay detection is 0.096 mIU/L of the sensitivity of TSH T4 sensitivity of 4.5 ng/ml, good linear relationship, the recovery rate of TSH between 91% and 101%, the recovery rate of T4 between 92% and 103%, test results are accurate. Conclusion the method of biochip chemiluminescence immune detecting a human serum TSH and T4 levels with high sensitivity, good linearity, high recovery rate and accuracy, worthy of attention and promotion.

评价BECKMAN ACCESS2全自动微粒子化学发光免疫分析系统相关性能。方法:以测定游离三碘甲状腺原氨酸(FT3)为例,收集患者高、中、低值新鲜血清混合标本,应用CLSI颁布的EP10-A2对ACCESS2全自动化学发光免疫分析系统进行初步评价。结果:通过CLSI EP10-A2程序评价显示ACCESS2检测系统偏差、不精密度、交叉污染、线性漂移等因素都在可接受范围内,对精密度无明显影响。结论:ACCESS2全自动发光免疫分析系统分析性能可靠,适合临床实验室使用。

Objective:To evaluate the performance of Beckman Access2 automated chemiluminescent immunoassay system in order to know its status. Method:Using the CLSI document EP10-2A for preliminary evaluation methods to approve the high,medium and low fresh serum of patients which were adopted as samples and was assayed FT3 in Beckman Access2 testing system.Result:The factors of carryover and drift,system deviation,imprecision,cross contamination in the ballpark,they had no effect on precision.Conclusion:The performance of Beckman Access2 automated chemiluminescent system is reliable and suitable for clinical laboratory to use.