利用高效液相色谱测定了三个地点的大豆品种合丰25与Bayfield及其衍生的144个重组自交系种子中的维生素E含量,运用主基因+多基因混合遗传模型,对三个地点的大豆种子的维生素E进行了遗传分析。结果表明:在哈尔滨地区α-生育酚为2对主基因+多基因遗传模型;γ-生育酚为1对主基因+多基因遗传模型;δ-生育酚为2对主基因+多基因遗传模型;在呼兰地区α-生育酚为3对主基因+多基因遗传模型;γ-生育酚为2对主基因+多基因遗传模型;δ-生育酚为2对主基因+多基因遗传模型;在绥化地区α-生育酚为无主基因+多基因遗传模型;γ-生育酚为3对主基因+多基因遗传模型;δ-生育酚为2对主基因+多基因遗传模型。

Vitamin E composition contents in soybean seeds Hefeng 25 and Bayfield,and their 144 recombi-nant inbred lines were determined by high performance liquid chromatography (HPLC)in 3 locations.Vitamin E contents were analyzed through mixed major gene plus polygene inheritance models in 3 locations.The results showed that α-tocopherol contents were 2 major genes plus polygene inheritance models,γ-tocopherol contents were 1 major genes plus polygene inheritance models andδ-tocopherol contents were 2 major genes plus polygene inheritance models in Harbin.The α-tocopherol contents were 3 major genes plus polygene inheritance models,γ-tocopherol contents were 2 major genes plus polygene inheritance models andδ-tocopherol contents were 2 ma-jor genes plus polygene inheritance models in Hulan.And α-tocopherol contents were without major genes plus polygene inheritance models,γ-tocopherol contents were 3 major genes plus polygene inheritance models andδ-tocopherol contents were

目前,医学界普遍承认治疗骨肉瘤的最佳方法是基因治疗,常用的有抑癌基因治疗、反义基因治疗、自杀基因治疗、免疫基因治疗、联合基因治疗等.但是无论哪种基因治疗方法,都必须具备安全的基因载体.骨肉瘤的基因治疗方法近几年有所突破,在广泛应用的基础上,还应进一步强调基因载体的重要性.

At present,the medical profession generally acknowledged the best way to treat osteosarcoma is gene therapy,which includes tumor suppressor gene therapy,antisense gene therapy,suicide gene therapy,immune gene therapy,combined gene therapy,etc.But no matter what kind of gene therapy is that the gene must have a safe carrier.Gene therapy has made a breakthrough in osteosarcoma recently.On the basis of widespread use,we should emphasize the importance of gene vectors.

针对高维小样本癌症基因数据集的有效区分基因子集选择难题,提出基于统计相关性和K-means的新颖混合基因选择算法实现有效区分基因子集选择.算法首先采用Pearson相关系数和Wilcoxon秩和检验计算各基因与类标的相关性,根据统计相关性原则选取与类标相关性较大的若干基因构成预选择基因子集;然后,采用K-means算法将预选择基因子集中高度相关的基因聚集到同一类簇,训练SVM分类模型,计算每一个基因的权重,从每一类簇选择一个权重最大或者采用轮盘赌思想从每一类簇选择一个得票数最多的基因作为本类簇的代表基因,各类簇的代表基因构成有效区分基因子集.将该算法与采用随机策略选择各类簇代表基因的随机基因选择算法Random,Guyon的经典基因选择算法SVM-RFE、采用顺序前向搜索策略的基因选择算法SVM-SFS进行实验比较,几个经典基因数据集上的200次重复实验的平均实验结果表明:所提出的混合基因选择算法能够选择到区分性能非常好的基因子集,建立在该区分基因子集上的分类器具有非常好的分类性能.

To deal with the challenging problem of recognizing the small number of distinguishable genes which can tell the cancer patients from normal people in a dataset with a small number of samples and tens of thousands of genes, novel hybrid gene selection algorithms are proposed in this paper based on the statistical correlation and K-means algorithm. The Pearson correlation coefficient and Wilcoxon signed-rank test are respectively adopted to calculate the importance of each gene to the classification to filter the least important genes and preserve about 10 percent of the important genes as the pre-selected gene subset. Then the related genes in the pre-selected gene subset are clustered via K-means algorithm, and the weight of each gene is calculated from the related coefficient of the SVM classifier. The most important gene, with the biggest weight or with the highest votes when the roulette wheel strategy is used, is chosen as the representative gene of each cluster to construct the d

目的对前期获得的3头转基因牛进行分子生物学评价，包括转基因阳性鉴定、基因及蛋白表达水平的检测，旨在进一步为转基因牛的安全评价提供一些科学依据。方法根据 GenBank 上公布的牛 FABP4基因（GenBank登录号：NM_174314）mRNA序列，利用兼并密码子对现有FABP4基因进行突变，并构建了pEGFP-C1-FABP4真核表达载体、通过细胞转染、体细胞核移植、胚胎移植技术获得3头转基因牛；分别采集了1号转基因牛不同组织样，2、3号转基因牛与野生型个体8、10、12、14月龄的血液样本，利用RT-PCR技术对所获转基因个体的阳性与否进行了鉴定；同时利用荧光定量PCR技术检测了1号转基因牛不同组织中外源性FABP4基因的表达情况，并分析了2、3号转基因牛在8—14月龄之间，外源性FABP4基因、内源性FABP4基因的变化趋势，并通过叠加转基因牛外源与内源性FABP4基因的表达量，分析了转基因牛和非转基因牛FABP4基因的整体表达水平差异；通过Western blotting技术分析了3头转基因牛外源性FABP4基因的蛋白表达水平。结果①对所获得的3头转基因牛RT-PCR检测发现，3头转基因个体各样本均在205 bp处产生条带，而妊娠母牛及阴性对照在205 bp处均未有条带出现，初步表明3头转基因牛均为转基因阳性。

Objective] The objective of this study is to identify 3 transgenic beifers cattle by testing mRNA and protein expression of exogenous FABP4 gene, so as to provide a scientific basis for the further study of the safety of genetically modified cattle.[Method] The bovine FABP4 gene was modified, which was obtained from GenBank (accession No. NM_174314), to form an exogenous FABP4 gene by the degeneracy of synonymous codon. Then the pEGFP-C1-FABP4 eukaryotic expression vector was constructed and transferred into recipient cows by somatic cell nuclear transfer and embryo transfer technology. The No.1 and 2 transgenic animals were born on July 19, 2012 and No.3 individual was on August 1, 2012, but No.1 individual was dead on July 20, 2012. The tissue samples of No.1 transgenic individual and blood samples of No.2&3 transgenic individual andBos Taurus at 8, 10, 12 and, 14 months were collected for identification. The RT-PCR was used to detect whether the transgenic individuals are

选择标记基因广泛应用于植物转基因工程中,然而这些基于除草剂和抗生素类抗性选择标记基因的利用,在转基因筛选和鉴定完成后功能多余。当前转基因植物的标记基因及相关产物对环境污染和食用安全的潜在影响,已经引起了人们的广泛关注。随着转基因技术的不断发展和人们对转基因植物和食品安全性的关注,删除转基因植物中的选择标记基因非常重要。文章着重讨论了植物转基因过程中,选择标记基因的利用,删除选择标记基因的几种方法,包括共转化法、位点特异性重组、染色体内重组、转座子介导的重组、删除叶绿体转化中选择标记基因,以及无选择标记基因植株的直接再生等。同时,还对这些技术的发展作了展望。

Selectable marker genes were widely used in the transgenic plants .However, these genes based on the re-sistance to antibiotic and herbicides are redundant since transgenic plants have been selected and identified .Due to the presence of selectable marker gene and its products , the environment pollution and food safety of transgenic plants are strongly concerned by consumers .With the development of transgenic technology , it is very important to eliminate the selectable marker gene in transgenic plants .In this review , utilization of selectable marker gene , methods of marker gene excision consisted of co-transformation , site-specific recombination , intrachromosomal recombination , recombination by transposon , removal of chloroplast marker gene , regeneration of direct marker-free plants, as well as the developmental directions of this technology were discussed .