为了探明油桐脂肪酸代谢机理的理论基础,以油桐优良品种葡萄桐的种子为试材,构建其转录组数据库,综合分析了转录组和表达谱的数据库,得到了与油桐脂肪酸代谢相关的非冗余基因序列共83条,涉及主要酶基因10种;同时以KEGG数据库为参考,对油桐脂肪酸代谢途径进行了分析,绘制了油桐脂肪酸代谢的路径图,揭示了油桐脂肪酸代谢途径中关键酶基因调控及催化脂肪酸代谢的基本规律。

In order to ascertain theoretical basis of fatty acid metabolism mechanism in Vernicia fordii, taking seeds of a ifne variety‘Grape Tong’ as materials, its transcriptome database was established, the transcriptome and expression spectrum database were comprehensively analyzed, total 83 non-redundant gene sequences were found, which involved in fatty acid metabolism in V. fordii and 10 main enzyme genes. Taking KEGG database as a reference, the fatty acid metabolism pathways in V. fordii were analyzed, and its pathway graph was drawn, which revealed the basic rules of key enzyme genes regulation and catalytic fatty acid metabolism in fatty acid metabolism pathways in V. fordii.

【目的】克隆油桐酸性磷酸酶（ACPase）基因的全长序列，为进一步分析油桐ACPase的功能奠定基础。【方法】根据油桐转录组酸性磷酸酶蛋白全长序列设计引物，利用RT-PCR从油桐种子中克隆ACPase基因，对其cDNA序列、核苷酸序列的相似性、理化性质、疏水性、跨膜结构、二级结构及三级结构进行分析预测，同时进行多序列比对并构建系统树。【结果】克隆获得油桐ACPase基因，其开放阅读框为1152 bp，编码383个氨基酸残基，相对分子质量为40.94 kDa，理论pI为5.30，属可溶性不稳定蛋白。同源性和进化树的预测分析结果显示，油桐ACPase与毛果杨的ACPase蛋白同源性达84%。油桐ACPase存在磷酸酶基因家族的保守区域，ACPase编码蛋白的大部分氨基酸属于亲水性氨基酸。其蛋白二级结构主要由不规则卷曲、α-螺旋和β-折叠构成。ACPase蛋白包含1个跨膜螺旋区域，其相应氨基酸位置在136~156；包含1个疑似跨膜域，其相应氨基酸位置在255~275；每个跨膜结构域为由20个氨基酸残基组成的螺旋，同时跨膜蛋白的N端和C端位于细胞膜胞质的一侧。【结论】油桐ACPase基因的表达可能与其体内磷营养的分解利用等过程有关。

[Objective]The full-length sequence of acid phosphatase(ACPase)gene in tung tree was cloned to provide ref-erences for further studies on the functions of ACPase. [Method]The primers were designed according to the full-length se-quence of acid phosphatase gene in transcriptome of tung tree. ACPase gene from tung tree seeds was cloned by RT-PCR. Bioinformatic analysis was used to predict cDNA sequence and its deduced nucleotide sequence similarity, physicochemical properties, hydrophobicity, transmembrane structure, secondary structure, and tertiary structure of ACPase. Multiple se-quences alignment and phylogenetic trees were also constructed. [Result]ACPase in tung tree was cloned. The largest open reading frame of ACPase had 1152 bp in length and encoded a polypeptide of 383 amino acid residues. The estimated molecu-lar weight and isoelectric point of the putative protein was 40.94 kDa and 5.30, respectively. The ACPase was a soluble unsta-ble protein. BLASTs search result and phylog

以三峡库区油桐幼苗为实验材料，在种植前喷施百草枯，并以喷施清水作为对照，比较研究百草枯对油桐幼苗生长及其土壤理化性质的影响．结果表明：处理组的土壤 pH 值比对照高1.0％，差异不显著（p ＞0.05）；处理组的土壤碱解氮、速效磷和速效钾含量分别比对照高6.8％，6.3％和5.4％，差异均不显著（p ＞0.05）；处理组的土壤有机质、全氮、全磷和全钾含量分别比对照低1.5％，2.0％，0.39％和6.5％，差异也均不显著（p ＞0.05）；处理组的油桐幼苗株高和地径增长量分别比对照组高11.8％和1.6％．可见，在正常使用剂量范围内，百草枯不仅对土壤的理化性质影响不大，还有助于油桐幼苗生长．因此，百草枯可用于消除油桐幼苗早期的杂草．

The change of soil physicalchemical properties and growth situation of Three Gorges Reservoir area Tung oil tree has been analyzed in this paper with spraying the appropriate concentration of paraquat and water before planting .Results indicate that the soil pH ,content of alkali‐hydrolysable nitrogen ,avail‐able phosphorus and available potassium of the treatment are respectively 1 .0% ,6 .8% ,6 .3% and 5 .4%higher than that in control ,no significant difference in the treatment and the control (p > 0 .05) ;soil or‐ganic matter ,total nitrogen ,total phosphorus and total potassium tested are 1 .5% ,2 .0% ,0 .39% and 6 .5% lower than the contrast ,differences are not significant (p > 0 .05) ;increment of height and basal di‐ameter of Tung oil tree plant treated are 11 .8% and 1 .6% higher than in control .Paraquat in a normal range not only has no obvious influence on the soil physical and chemical properties ,also is helpful to Tung oil tree growth .Therefore ,paraquat c

为了揭示油桐乙酰辅酶A羧化酶accA基因的序列特点和结构功能,从而为该基因的深入研究和开发利用奠定基础,以葡萄桐的近成熟种子为材料,根据油桐转录组测序结果设计引物,采用RT-PCR技术克隆了油桐乙酰辅酶A羧化酶α亚基全长cDNA序列。测序结果表明：该基因cDNA序列全长2 313 bp,编码770个氨基酸;以其推导出的α亚基氨基酸序列蛋白的等电点pI为8.48,相对分子量为85 902.7 Da,稳定系数为37.33。生物信息学分析结果表明：此蛋白含有4个比较明显的跨膜区,是一个不稳定的非分泌蛋白。模体搜索结果表明：在α亚基上有羧基转移酶域和ACCA功能域;其三级结构中有14个α螺旋,整体呈球型,并有一个向外延伸的结构。该基因已登录到GenBank,命名为VfCTα。

In order to reveal sequence features and structural function of accA gene in Vernicia fordii, and to lay a foundation for further research, development and utilization, taking nearly ripe seeds in V. fordii as materials, the speciifc primers were designed according to the results of transcriptome sequencing analysis of V. fordii, and full-length cDNA sequences of accA gene were cloned by RT-PCR technique. The results show that full-length cDNA sequence of accA gene is 2 313bp, encoding 770 amino acids. Iso electric point (pI) of accA protein is 8.48, the relative molecular mass is 85 902.7 Da, and the stability coefifcient is 37.33. The results of bioinformatics analysis show that, the protein has four obvious trans-membrane domains, and is an unstable non-secretory protein. The results of motif research show that a Carboxyl transferase domain and a ACCA functional domain are located inαsubunit. The tertiary structure of this protein has 14αhelixes, and appears a spherical

香椿造林中通过实施不同基肥的处理,对其造林效果进行了试验研究,结果表明,施用基肥第3年地(胸)径、树高生长量分别比对照提高70%~98%和42.8%~114.3%;第5年胸径、树高生长量分别比对照提高22%~31%和48%~56%,施用有机质含量高、肥效长的油桐饼,效果最佳。

Toona sinensis forestations were treated with different basic fertilizers .The survey results showed that tree (chest) diameter and height growth mass were increased by 70% ~ 98% and 42 .8% ~ 114 .3% compared with the control in the third year of fertilizers application ;in the fifth year ,they were increased respectively by 22% ~ 31%and 48% ~ 56% compared to the control .Application of tung cake with high organic matter content and long-term fertilizer efficiency brought the best effect .