以海藻酸钠为原料、氯化钙为交联剂,反应直写制备海藻酸钙水凝胶中空纤维及组织工程支架。纤维的凝胶率和溶胀度直接影响支架的成形质量与所装载细胞的存活率。浸取分离所直写中空纤维的未交联部分,分析材料浓度对凝胶率的影响;以滴注凝固浴制备的海藻酸钙微球为对照组,分析直写工艺的溶胀度表现。结果表明,以4%海藻酸钠与3%氯化钙材料交联直写的中空纤维凝胶率和纤维形貌较好地满足了直写工艺的要求;随着海藻酸钠浓度的增加,中空纤维的溶胀度先减小后小幅升高并趋于稳定;随着氯化钙浓度的增加,凝胶纤维的溶胀度逐渐减小;反应直写工艺由于较小的两相接触表面,其溶胀度比凝固浴制备凝胶微球高2～5倍,更高的含水率有利于保障所装载细胞的活性和传质。

With sodium alginate as raw material and calcium chloride as crosslinker, a hollow hydrogel fiber was fabricated by direct writing combined with crosslinking, and was used to construct tissue engineering scaffold. Fiber gel fraction and swelling degree directly affect the forming quality of scaffold and laden cell survival. The influence of materials concentration on gel fraction was analyzed by leaching the ungelled part from the fiber. As a control group, alginate beads were prepared with coagulating bath. The requirements of direct writing process, such as gel rate, gel fraction and fiber morphology were satisfied, when the concentration of sodium alginate and calcium chloride were 4% and 3% respectively. With increasing sodium alginate’s concentration, equilibrium swelling degree of hollow fiber first decreased and then slightly increased and stabilized finally. With increasing calcium chloride’s concentration, swelling degree gradually decreased. Due to smaller diffusi

背景:海藻酸纤维是一种多形式且多功能性物质,目前已被逐步应用于生物医学及临床.目的:全面评述海藻酸纤维的结构与功能,同时介绍海藻酸与壳聚糖、海藻酸与明胶共混纤维的特性及在医学上研究的应用情况.方法:由第一作者检索1990至2012年 PubMed 数据库及万方数据库有关海藻酸纤维制作过程、海藻酸纤维结构及性能、海藻酸纤维在生物医学的应用情况及其相关共混纤维的特性与应用等方面的文献.结果与结论:海藻酸盐已被广泛应用于农业化工、生物防治、组织工程、缓释药物系统、创伤修复、净化环境等方面.通过共混改性得到的各种新型混合纤维,不但改善了海藻酸纤维应用上的不足,同时也赋予其更多的性能,成为了当今和未来生物材料的研究热点.目前生物医学材料正向着环保、高功能、智能化等方向发展,自然界储存量丰富、成本价格低廉、具有良好生物相容性及降解性的海藻纤维、壳聚糖纤维和明胶纤维及以它们为基质通过共混和/或改性等方法制造出来的功能性纤维潜力巨大,在生物医学及仿生医学领域有待进一步开发.

BACKGROUND: At present, alginate fiber possesses various forms and functions, which is increasingly used in biomedicine and clinical medicine. OBJECTIVE: To comprehensively review the structure and feature of alginate fiber and to introduce the biomedical application of its blend fiber with chitosan or gelation. METHODS: PubMed database and Wanfang database were retrieved by the first author for literature about alginate fiber production process, the alginate fiber structure and properties, biomedical application of alginate fiber, and related blended fiber characteristics and applications, published from 1990 to 2012. RESULTS AND CONCLUSION: Alginate has been widely applied in agriculture and chemical industry, biological control, tissue engineering, drug delivery system, wound repair, and environmental purification. New hybrid fibers obtained through blending modification cannot only improve the shortcomings of alginate fiber applications, but also be endued with more performances, a

综述了海藻酸纤维的结构、制备方法及应用性能，重点介绍了喷涂浸渍法、离子交换法、在位还原法和共混纺丝法等海藻酸纤维载银技术及相关研究进展，并分析了不同载银技术的优势及存在的问题，提出了海藻酸纤维载银技术的未来发展方向。

This paper highlights the structure, preparing method and applying performance of the alginate fiber. It emphatically introduces the Ag-loading technology and its related research, including spray impregnating method, ion exchanging method, in situ method and blend spinning method, and analyzes the advantages and disadvantages of different Ag-Loading technology. It also proposed the direction of future development of Ag-loading technology of alginate fiber.

背景：课题组和青岛大学高分子材料研究所合作研制的海藻纤维生物膜，具有优良的生物相容性，常被用作制备各种复合材料。目的：观察海藻纤维膜片包绕覆盖神经断端吻合口对大鼠坐骨神经损伤后再生的影响。方法：切断36只雄性Wistar大鼠右侧坐骨神经，随机分组：对照组行神经外膜端端吻合；实验组行神经外膜端端缝合，将海藻纤维膜片包绕并覆盖神经吻合口远近端各约0.5 cm，形成封闭再生室。术后观察海藻纤维膜片降解吸收规律及缝合处粘连情况，组织学切片行苏木精-伊红染色、锇酸染色、白细胞介素2及白细胞介素4免疫组织化学染色。结果与结论：术后4-6周，实验组海藻纤维膜片逐渐被降解吸收，与周围组织粘连较少，炎性细胞浸润程度较轻，纤维组织增生较少。两组术后1，7，14 d的白细胞介素2及白细胞介素4含量比较差异无显著性意义。实验组术后6周再生神经纤维分布规则且大小较为均一，其神经纤维数量、轴突大小及髓鞘厚度等指标均显著优于对照组(P <0.05)。表明海藻纤维膜片具有良好的生物降解性和组织相容性，其包绕覆盖坐骨神经形成的神经再生密闭室可促进大鼠损伤坐骨神经再生。

BACKGROUND:The sodium alginate film, jointly developed by our research team and the Institute of Polymer Material of Qingdao University, has good biocompatibility and is often used for the preparation of a variety of composite materials. OBJECTIVE: To observe the effect of sodium alginate film wrapping and covering nerve anastomotic stoma on the regeneration of injured sciatic nerve in rats. METHODS: A total of 36 male Wistar rats were randomly divided into two groups after the right sciatic nerve was cut. Control group: The nerve stump was sutured through epineurial end-to-end anastomosis; Experimental group: The nerve stump was sutured through epineurial end-to-end anastomosis, and wrapped with sodium alginate film, forming a regenerative environment for sciatic nerve regeneration. The degradation and absorption of sodium alginate film, as wel as the adhesion at suture site were observed post-operation. Interleukin-2 and interleukin-4 positive expression was detected with immunohisto

分别使用具有相似羧基含量的海藻纤维、丙烯酸接枝改性聚四氟乙烯纤维和聚丙烯纤维(PAA-g-PP和PAA-g-PTFE)3种含羧酸纤维与Fe3+进行配位反应,研究和比较了反应的动力学特性及影响因素.将3种含羧酸纤维铁配合物分别作为非均相Fenton反应催化剂应用于染料降解反应中,分析和评价了其配位结构和表面性能对催化活性的影响.结果表明,在所涉及的温度和浓度范围内,3种含羧酸纤维与Fe3+的反应均很好地符合Langmuir等温吸附模型和Lagergren准二级动力学方程.Fe3+初始浓度的增加会降低反应速率常数,而反应温度的升高则会增加配合物中Fe3+的配合量.在相同反应条件下,海藻纤维比PAA-g-PP和PAAg-PTFE更容易与Fe3+发生反应,且反应速率常数和Fe3+配合量按照下列顺序排列:海藻纤维PAA-g-PPPAA-g-PTFE.3种含羧酸纤维铁配合物都能够在染料氧化降解反应中作为非均相Fenton催化剂,且紫外光比可见光更能够提高其催化活性.海藻纤维铁配合物比其它2种含羧酸纤维铁配合物具有更好的催化作用,这与三者在配位结构和表面性能之间的显著差异有关.

Three carboxylic fibers including alginate fiber, polyacrylic acid grafted polypropylene and polyte-trafluoroethylene fibers ( PAA-g-PP and PAA-g-PTFE ) with similar carboxyl contents were coordinated with Fe3+, respectively to prepare the different Fe (Ⅲ)-carboxylic fiber complexes. The coordinating kinetics of three carboxylic fibers with Fe3+was compared, and the effecting factors were also examined. And the catalytic performance of three Fe(Ⅲ)-carboxylic fiber complexes was then evaluated as the heterogeneous Fenton cata-lysts in the dye degradation in water. The results indicated that within the observed temperature and concentra-tion range, the coordination of carboxylic fiber with Fe3+showed better agreement with Langmuir isotherm equa-tion and Lagergren second order equation. Increasing Fe3+initial concentration led to a low coordination rate constant. Higher temperature increased the Fe content of the resulting complexes. Alginate fiber reacted more easily with tha