目的:探讨溶血对生化检验结果准确性的影响及校正方法。方法随机抽取健康体检者26例,采集静脉血,在不同溶血程度的情况下,进行生化检测并比较分析。再对血清 Hb 浓度与受溶血影响较大的部分生化指标变化值进行回归分析。结果标本溶血对 tBil、dBil、ast、alt、ldH、cK、cK-MB、K、na 结果有明显影响,经回归分析, tBil、dBil、ast、alt、ldH、cK、cK-MB、K、na 的溶血变化值与血清 Hb 浓度变化值的相关系数分别为0.974、-0.978、0.980、0.978、0.984、0.988、0.980、0.986、-0.991。结论溶血可以引起多项生化指标测定结果明显改变,并与血清 Hb 浓度有相关性,采用回归分析后,可以有效地对结果进行校正。
Objective to investigate the effect of hemolysis on biochemical test results accuracy and correction method. Method randomly selected 26 cases of healthy people, collecting venous blood hemolysis degree, in different circumstances, biochemical test and the comparative analysis. the serum concentration of Hb and the hemolytic effect of partial biochemical index changes larger values by regression analysis. Results the hemolysis of tBil,dBil,ast,alt,ldH,cK, cK-MB,K,na results are obvious effect, regression analysis, tBil,dBil,ast hemolytic changes, alt,ldH,cK,cK-MB,K,na value of the correlation coefficient and serum Hb concentration were 0.974, - 0.978,0.980,0.978,0.984,0.988,0.980,0.986,-0.991.Conclusion Hemolysis can be caused by a number of biochemical index determination results change significantly, and has correlation with serum Hb concentration, using regression analysis, can effectively correct the result.