生育三烯酚是一种高品质的天然维生素E,具有广阔的开发前景和市场需求。介绍了生育酚及生育三烯酚的来源,重点论述了生育酚及生育三烯酚分离纯化的常用方法、相关技术路线及其对应的优缺点和适用范围。结果表明:离子交换法选择性高,特异性强,适合提取高含量的生育酚及生育三烯酚;柱色谱法仪器设备简单,理论上是分离生育酚和生育三烯酚的有效方法,但普遍存在洗脱时间过长、溶剂消耗量大等问题;超临界流体色谱在制备生育三烯酚及其单体方面具有良好的应用前景。最后介绍了当前国内外生育三烯酚的产业化情况,以期为今后国内生育三烯酚深入研究提供一定的参考。

The tocotrienols are a kind of natural vitamin E of high quality with wide development fore-ground and market demand. The sources of tocopherols and tocotrienols were introduced, the common separation and purification methods,the relevant technical route,the corresponding advantages and disad-vantages and application scope of these methods and routes were emphatically introduced. The results showed that ion exchange method had high selectivity and specificity, which was suitable for extracting high content tocopherols and tocotrienols;the instrument equipment of column chromatography was sim-ple,it was an effective method for the separation of tocopherols and tocotrienols in theory,but it had some problems such as long elution time and high solvent comsumption;supercritical fluid chromatograply had a good application prospect on preparing tocotrienols and its monomer. Finally,the industry situations of to-cotrienols at home and abroad were introduced to provide references for further r

采用甲酯化、冷冻结晶离心、分子蒸馏工艺提取脱臭馏出物中维生素E,其生育酚总损失率为10%~12%;研究工艺条件对生育酚损失影响,并对降低生育酚损失方法进行分析和探讨.

This article is mainly focusing on the study of tocopherol loss during the process of vitamin E purification from deodorization distillate. Total tocopherol loss is within a scope 10~12%. Meanwhile the study is also analyzing the influencing factors of tocopherol loss in the process of methyl–esterification,refrigeration,crystallization,separation,and molecular distillation,and discussing the methods to reduce down the loss of tocopherols.

通过对脱色温度、脱色时间、脱色白土用量3个因素进行单因素及正交实验,研究脱色条件对玉米油生育酚含量的影响。结果表明:对生育酚含量影响的因素依次为脱色时间脱色白土用量脱色温度,当脱色时间为20 min,脱色白土用量为2.5%,脱色温度为105℃时,生育酚损失最少,损失率为9.3%,此时脱色油的色泽为Y35R3.2,色泽达到二级油标准。

The bleaching temperature,bleaching time,decolorization clay dosage were researched by single factor test and orthogonal test,to research the loss of corn oil tocopherols during bleaching. The effects of the variables on the tocopherols content decreased in the order:bleaching time>decolorization clay dosage>bleaching temperature. The results showed that under the condition of the bleaching temperature 105℃,bleaching time 20 min,decolorization clay dosage 2.5%,the loss of tocopherolswas 9.3%,and the decolorization oil color was Y35R3.2. The color reached the secondary oil standard.

维生素E(VE)具有提高人体免疫力、抗癌、预防心血管疾病等保健作用,从大豆中提取的VE安全性更高。本研究采用高效液相色谱技术(HPLC)检测大豆BIEX群体(Essex×ZDD2315)维生素E的α-生育酚、γ-生育酚和δ-生育酚含量。应用QTLNetwork 2.1软件分别检测到8个和12对控制大豆维生素E及组分含量的加性和互作QTL。α-生育酚含量加性和互作QTL累计贡献值分别为8.68%(2个)和15.57%(4对),γ-生育酚含量加性和互作QTL累计贡献值分别为8.59%(2个)和11.57%(2对),δ-生育酚含量加性和互作QTL累计贡献值分别为5.44%(1个)和17.61%(3对),维生素E总含量的加性和互作QTL累计贡献值分别为11.39%(3个)和9.48%(3对)。未检测到维生素E及组分含量和环境互作的QTL。未定位到的微效QTL累计贡献值为66.16%~75.32%,说明未定位到的微效基因的变异占2/3以上。各性状的遗传构成中,未检测出的微效QTL份额最大,加性QTL和互作QTL贡献相差不大。在育种中应考虑常规方法聚合微效QTL与标记辅助方法聚合主要QTL相结合。

Vitamin E has effects on human immunity, anti-cancer and prevention of cardiovascular disease. Vitamin E from soy-bean has the advantages of higher security and higher human body absorption rate. The objective of the present study was to map the additive, additive × additive (epistasis), additive × year and epistasis × year QTLs for vitamin E and relative tocopherol con-tents with the RIL population BIEX (Essex×ZDD2315) using HPLC (high performance liquid chromatography) method and soft-ware QTLNetwork 2.1. Eight additive QTLs and twelve additive × additive (epistasis) QTLs were detected for vitamin E and relative tocopherol contents. The contributions to the phenotypic variances of additive QTL and epistatic QTL pairs were 8.68%(two QTLs) and 15.57% (four pairs) for α-tocopherol, 8.59% (two QTL) and 11.57% (two pairs) for γ-tocopherol, 5.44% (one QTL) and 17.61% (three pairs) for δ-tocopherol and 11.39% (three QTL) and 9.48% (three pairs) for total vitamin E contents

目的应用高压液相色谱法同时测定人乳中视黄醇、α-生育酚、叶黄素和β-胡萝卜素。方法人乳样品经皂化后,石油醚乙醚提取,氮吹浓缩后,用C30柱分离,经紫外检测器和荧光检测器检测4种被测物。结果 4种脂溶性维生素在各自浓度范围内线性良好,相关系数均大于0.9990;三浓度水平加标回收率在70%~120%之间,相对标准偏差均小于20%。结论本文将校准后的标准品用液相色谱法进行纯度鉴定,提高了检测的准确度。通过方法学验证了本方法的准确性,灵敏性和稳定性,可同时测定人乳中视黄醇,α-生育酚,叶黄素和β-胡萝卜素的含量。通过对100个成熟乳样品的分析,初步建立了人乳中脂溶性维生素含量数据库。

Objective To establish a rapid method for simultaneous determination of retinoid,α-tocopherol, lutein andβ-carotene in human milk by high performance liquid chromatography. Methods The human milk sample was saponified and extracted with a mixture of petroleum ether and diethyl ether. The sample was separated after concentration by a C30 column and detected by the UV detector and FLD detector. Results The method showed a good linearity over with R2>0.9990. The recoveries at three spiked levels were between 70%~120%. The relative standard deviation was less than 20%. Conclusion The purity of standard was analyzed using HPLC after standardization with UV spectrometer, thus the accuracy of the method was improved. The method validation showed a high accuracy, sensitivity and stability. This method could be used to simultaneous determination of retinoid, α-tocopherol, lutein and β-carotene in human milk. A preliminary database of the fat-soluble vitamin concentration in human