目的建立双波长高效液相色谱法同时检测穿心莲浸膏中穿心莲内酯和脱水穿心莲内酯的含量测定方法。方法采用Intersil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇—水(60∶40)为流动相;检测波长穿心莲内酯为225 nm,脱水穿心莲内酯为254 nm;柱温为35℃;流速为1.0 mL·min-1。结果穿心莲内酯进样量在0.1043~1.0430μg范围内与峰面积呈良好的线性关系(r=1.000 0,n=5),脱水穿心莲内酯进样量在0.099 9~0.999 0μg范围内与峰面积呈良好的线性关系(r=1.000 0,n=5);穿心莲内酯的平均加样回收率为98.53%(RSD=1.27%,n=6),脱水穿心莲内酯的平均加样回收率为101.51%(RSD=1.10%,n=6)。结论该方法灵敏度高,重现性好,能准确测定穿心莲浸膏中穿心莲内酯和脱水穿心莲内酯的含量。

Objective To establish a dual-wavelength HPLC method for the determination of andrographolide and dehydroandrographol -ide in extractum andrographis .Methods The analysis was performed on Intersil C 18 column(250 mm ×4.6 mm,5μm),using metha-nol-water (60∶40)as mobile phase,the UV detector wavelength set at 225 nm for andrographolide and 254 nm for dehydroandrographol-ide.Column temperature was maintained at 35 ℃and the flow rate was 1.0 mL· min-1 .Results There was good linearity within the range of 0.104 3~1.043 0μg (r=1.000 0,n=5)for andrographolide and 0.099 9~0.999 0 μg (r=1.000 0,n=5)for dehydroan-drographolide.The average recoveries of andrographolide and dehydroandrographolide were 98.53%(RSD=1.27%,n=6)and 101. 51%(RSD=1.10%,n=6).Conclusions This method is highly sensitive,rapid and accurate for determination of andrographolide and dehydroandrographolide in extractum andrographis .

目的：以中成药“消炎利胆片”为考核样品,统计了能力验证项目NIFDC-PT-005中来自30个省（市）、自治区的139家实验室的穿心莲含量测定结果,并对离群数据进行了技术分析。方法依据 CNAS-GL02,以“穿心莲内酯”、“脱水穿心莲内酯”以及“穿心莲内酯加脱水穿心莲内酯”总量为评价指标,对各参加实验室的能力验证结果进行了结果总数、中位数、标准四分位数间距、稳健的变异系数、最小值、最大值、极值、Z比分数的统计分析。评定标准：当｜Z｜≤2时,为满意结果；当2〈｜Z｜〈3时,为可疑结果；当｜Z｜≥3时,为不满意结果。结果“穿心莲内酯”、“脱水穿心莲内酯”以及“穿心莲内酯加脱水穿心莲内酯”总量3个指标均为满意结果的实验室有117家,占84.2%。结果可疑或者不满意的原因包括：供试品未去包衣,中性氧化铝的适宜性未验证,色谱分离欠佳,计算错误等。结论多数实验室的能力验证结果为“满意”,导致结果“可疑”或者“不满意”的主要因素为技术因素。

Objective To evaluate the results from the 139 laboratories in 30 provinces obtained in the proficiency testing program NIFDC-PT-005, which was intended to assess the proficiency of the laboratories in the determination of Xiaoyanlidan tablet.Technical analysis was also performed for outlier results. Methods Statistic analysis was performed on the results of andrographolide, dehydroandrographolide (quality-indicating compounds of Xiaoyanlidan tablet)and their total amount, including their total number, median, norm IQR (InterQuartile Range ), robust CV (coefficient of variation),minimum,maximum, range and Z-score. Evaluation criteria:if the absolute value of the Z-score is not more than 2 (|Z|≤2),the result is satisfactory;if the |Z| value is between 2 and 3 (2<|Z|<3),the result is questionable;if the |Z| value is not less than 3 (|Z|≥3),the result is unsatisfactory.Results Among 139 laboratories,117 ones (84.2%)obtained satisfactory results of andrographolide,dehydroandrog

目的对穿心莲药材的有效成分进行定性定量鉴别,对水分、总灰分、酸不溶性灰分、浸出物及重金属含量进行定量测定,进一步完善穿心莲药材的质量标准。方法采用薄层色谱法对穿心莲中的穿心莲内酯和脱水穿心莲内酯进行定性鉴别,高效液相色谱法进行定量测定,常规检查项目按2010年版《中华人民共和国药典》（一部）附录ⅨA杂质检查法、ⅨH水分测定法中的烘干法、附录ⅨK灰分测定法、附录ⅩA浸出物测定法及ⅨE重金属检查法测定。结果 10批穿心莲样品中穿心莲内酯和脱水穿心莲内酯及醇溶性浸出物含量均符合我国药典规定的标准。结论所建立的方法简便、准确,能作为质量标准以控制穿心莲药材的质量。

Objective To improve the quality standard of Andrographis Herba through determination of effective components, moisture, total ashes, acid insoluble ashes, extracts and heavy metals. Methods TLC and HPLC were used for qualitative and quantitative identification of andrographolide and dehydroandrograpolide in Andrographis Herba. Routine examinations were based on the procedures recorded in the Appendix Ⅸ A, Ⅸ H, Ⅸ K, ⅩA and Ⅸ E of Chinese Pharmacopoeia (2010), for foreign matter, moisture, ashes, extracts determination and heavy metal test respectively. Results Total content of andrographolide and dehydroandrographolide, extractives (70% ethanol) all complied with Chinese pharmacopoeia. Conclusion The established method was simple, accurate and can be used as the quality standard for the quality control of Andrographis Herba.

目的：探讨穿心莲内酯对新西兰兔实验性高脂血症调血脂的作用。方法：SPF级新西兰家兔雌雄各半36只，随机分为6组：正常对照组、模型对照组、非诺贝特组、穿心莲内酯高、中、低剂量组，对照组给予普通饲料饲养，其余各组给予高脂饲料50g，造模喂养4周后，按照分组继续进行高脂饲料喂养，对照组同法灌胃给予等体积蒸馏水，连续给药4周。末次给药后1小时耳动脉采血，测定血清血脂水平。结果：与模型对照组相比，穿心莲内酯高、中、低剂量组新西兰兔血清TC含量明显降低（P〈0．01），穿心莲内酯高、中剂量纽新西兰兔血清TG、LDL—C含量明显降低（P〈0．01），穿心莲内酯高、中、低剂量组新西兰兔血清HDL．C含量明显上升（P〈0．01）。结论：穿心莲内酯能明显改善新西兰兔实验性高脂血症的血脂水平。

Objective: To investigate the effects in andrographolide regulating blood lipid on experimental hyperlipemia of New Zealand rabbits. Methods:SPF New Zealand rabbits, half male and female of 36, were randomly divided into 6 groups:normal control group, model control group, fe-nofibrate group, andrographolide group of high, medium and low dose, control group was given ordinary feed, the others were given high-fat diet 50g, after feeding 4 weeks, control group with intragastric administration of distilled water, the others continued high-fat diet in accordance with grouping, with successive administration of 4 weeks. Serum lipid levels were determinated by ear arterial blood after 1h of last administration. Re-sults:Comparing with model group, serum TC of New Zealand rabbits in andrographolide group of high, medium and low dose decreased significant-ly (P<0.01), as well as serum TG, LDL-C of New Zealand rabbits in andrographolide group of high and medium dose (P<0.01), serum HDL-C of New

在前期筛选具有抗猪繁殖与呼吸综合征病毒(PRRSV)作用的中药研究基础上,选取药效较好的三种中药:穿心莲、芦根和夏枯草。为进一步研究其有效抗病毒成分,采用MTT法观察了穿心莲内酯盐、芦根多糖和夏枯草多糖成分对猴肾上皮细胞系(Marc-145)生长的影响,分别采用阻断、抑制、直接杀灭3种作用方式观察了各成分抗RPPSV感染作用。结果显示:穿心莲内酯盐对细胞最大安全浓度为0.16 mg/mL,芦根多糖为0.575 mg/mL,夏枯草多糖为1.125 mg/mL;芦根多糖在三种方式上均有不同程度的抗PRRSV作用,而穿心莲内酯盐和夏枯草多糖阻断PRRSV作用不明显,抑制和杀灭PRRSV作用明显。结果表明穿心莲内酯盐、芦根多糖和夏枯草多糖均有抗病毒作用,为抗病毒中药的主要药效成分。

Andrographolide, phragmites polysaccharide and selfheal polysaccharide were selected to study the antiviral action against to porcine reproductive and respiratory syndrome virus( PRRSV) in vitro. The blocking, inhibiting and killing roles of these extract were detected in Marc-145 cells infected with PRRSV. Results showed the safety concentration of andrographolide, phragmites polysaccharide and selfheal polysaccharide respectively are:0.16, 0.575 and 1.125 mg/mL. All the 3 extracts had antiviral actions against to PPRSV by inhibiting and killing roles,but just phragmites polysaccharide has the blocking role in highest safety concentration. And the way of extract acting in protection of the cell was as the same as the whole herb, indicate these extract are the major portion of the Chinese medicine herb.