考察芥子碱氯化盐对家兔离体肠平滑肌收缩功能的影响。方法:以家兔离体肠平滑肌收缩频率和张力为指标,采用Medlab-U/4C501H生物机能实验系统,考察不同浓度芥子碱氯化盐对家兔离体肠平滑肌收缩功能的影响。采用M受体阻断剂(阿托品)孵育肌条,观察芥子碱氯化盐在M受体阻断剂存在下对家兔肠管平滑肌收缩效应的变化。结果:各个实验浓度范围的芥子碱氯化盐对家兔离体肠平滑肌的收缩频率均无明显影响(P0.05),各实验浓度芥子碱氯化盐能够显著提高家兔离体肠管的张力(P0.01);M受体阻断剂阿托品对芥子碱氯化盐引起的肌条收缩有抑制作用,但抑制作用不完全。结论:芥子碱氯化盐对家兔离体肠管平滑肌具有兴奋作用,其兴奋肠管的作用与激动M受体有关。

Objective:To investigate the effect of sinapine chloride on the contractility of isolated ileum smooth muscle of rabbits .Methods:Use the contraction frequency and tension of muscle strips as indexes to investigate the effect of different doses of sinapine chloride on the contractility of isolated ileum smooth muscle , and the contraction information was recorded with Medlab -U/4 C501 H biological function experimental system .The muscle strips were incubated with M receptor blocker ( atropine ) to observe the effects of sinapine chloride con-tractile activity in the presence of atropine .Results:All doses of sinapine chloride had no obvious effect on the contraction frequency of isolated ileum smooth muscle strips ( P>0 .05 ) .All doses of sinapine chloride could obviously increase the tension of muscle strips ( P<0 .01 ) .M receptor blocker ( atropine ) could inhibit the contraction effect caused by Sinapine but the effect was incomplete .Conclusion:Sinapine chloride has an ex-citato

优选咳喘三伏贴处方中芥子碱硫氰酸盐的超声波提取工艺。方法:采用HPLC梯度洗脱法测定芥子碱硫氰酸盐的含量,以芥子碱硫氰酸盐的提取率为指标,通过正交试验优化超声波提取的工艺。结果:最佳提取工艺为混合药材用35倍的60%乙醇提取2次,超声频率为28kHz。结论:优选的工艺提取效率高,稳定可行。

OBJECTIVE To optimize the ultrasonic extraction process of sinapine thiocyanate from Kechnansanfutie formula.METHODS Sirmpine thiocyanate was determined by HPLC with gradient elution,and the optimal extracting process was obtained via an orthogonal test method according to the extracting efficiency of sinapine thiocyanate.RESULTS The optimal extracting process was adding 60% alcohol thirty-five times as the total weighty of Chinese materials and extracting for two times with 28 kHz uhrasound.CONCLUSION The extracting rate is high,and the process is stable.

目的建立通络糖泰颗粒的质量标准。方法采用薄层色谱法对制剂中黄连、芥子、玄参进行定性鉴别,采用高效液相色谱法测定通络糖泰颗粒中盐酸小檗碱含量。结果黄连、芥子、玄参薄层色谱斑点清晰,专属性强,阴性对照无干扰。盐酸小檗碱进样量在0.037 29~2.610 3μg范围内呈良好的线性关系,r=1.000 0,平均回收率为98.85%,RSD=0.70%(n=6)。结论该方法灵敏、简便、准确、重复性好,可作为通络糖泰颗粒的质量控制标准。

Objective To establish the quality standard for Tongluo Tangtai Granules. Methods TLC was applied to the qualitative identification of Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae, and HPLC was applied to the quantitative determination of berberine in the prescription. Results Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae could be detected by TLC, the spots were clear and there was no interference for the negative sample. A good linearity of berberine was showed in the range of 0.037 29-2.610 3 μg (r=1.000 0). The average recovery of berberine was 98.85%, and RSD was 0.70% (n=6). Conclusion The methods are simple, sensitive, accurate with good reproducibility. It can be used for the quality control of Tongluo Tangtai Granules.

芦竹作为一种多年生的能源植物，具有广阔的开发利用前景。以傅里叶变换拉曼光谱技术对苯醇抽提前后芦竹原料、磨木木质素以及不同碱处理时间获得的碱木质素进行了无损分析。研究结果表明，原料中苯醇抽出物的存在会降低芦竹拉曼特征峰的强度，但不产生新的拉曼特征峰。芦竹磨木木质素的拉曼光谱图表明芦竹木质素为SGH 型木质素。相比于木材磨木木质素，芦竹磨木木质素在1173 cm-1处的特征峰更强。通过对羟基肉桂酸标准物的拉曼光谱分析，进一步证实了该特征峰可能由草类原料中的羟基肉桂酸引起。此外，碱木质素拉曼光谱分析结果表明，碱处理40 min获得的木质素中羟基肉桂酸特征峰最强，松柏醛/芥子醛特征峰最弱，而松柏醇/芥子醇的特征峰几乎消失。可见，该碱木质素中酚酸含量最高，潜在的抗氧化性最好，利用价值最高。二维异核单量子碳氢相关谱进一步验证了所得结果，证实了傅里叶变换拉曼光谱技术可作为一种安全、快速、准确、无损的分析手段鉴定木质素结构。

Arundo donax linn,as a perennial energy crop,has promising application prospect.In the present study,Fourier transform Raman (FT Raman)spectroscopy was applied to determine the structural information of materials,milled wood lignin (MWL),and alkaline lignins (AL,under different treated time)from A. donax stem nondestructively.The results indicated that,extractable compounds in A. donax had negative contribution to the Raman spectra without rising new Raman peaks.FT Raman spectrum of MWL indicated that MWL from A. donax was HGS type lignins.Compared with the spectra of MWL from wood materials,the peak at 1 173 cm-1 was much higher in intensity for the MWL from A. donax stem,which may be assigned to hydroxycinnamic acid by analyzing the standard.With respect to FT Raman spectra of ALs,the relatively highest intensity of 1 173 cm-1 was found in alkaline lignin (AL2 ),which was treated for 40 min by alkaline.Moreover,the peak of coniferalde-hyde/sinapaldehyde (1 630 cm-1 )was lowest in int

芥子酶能分解底物硫代葡萄糖苷产生萝卜硫素等抗癌物质。本研究拟从十字花科植物寄生菜蚜体内分离产芥子酶微生物。通过RSM培养基富集培养,氯化钯-硫苷降解率法初筛,葡萄糖产量法复筛。分离获得9株产芥子酶菌株,其中DS12菌株的酶活最高,为1.486 IU;并且在VC浓度为0.28μg/uL时,酶活最大;在发酵42 h,生长量最大。DS12可作为生产芥子酶的潜力菌株。

Myrosinase can degrade glucosinolates to produce sulforaphane and other anti-cancer materials. This study aimed to isolate myrosinase producing microorganisms from cabbage aphid from Lipaphis erysimi that specifically parasitizing Brassicaceae plants. Throngh enrichment culture with RSM medium , screening with palladium chloride-the glucosinolate degradation rate and rescreening with glucose production method , 9 strains producing myrosinase were isolated in this paper , in which the D12 strain has the most enzyme amounts up to 1.486 IU, its enzyme activity is most at VC concentration 0.28 μg/μL, its maximum amount of growth arised at fermentation time 42 hours. So DS12 has the potential for producing myrosinase.