目的探讨荜茇明碱(PL)对人乳腺癌MDA-MB-231细胞增殖、凋亡的影响及作用机制。方法体外培养人乳腺癌MDA-MB-231细胞;采用活细胞计数试剂盒(CCK-8)法检测不同浓度的PL对MDA-MB-231细胞增殖的影响;流式细胞术检测PL对细胞凋亡的影响;蛋白免疫印迹(Western blot)法检测PL对MDA-MB-231细胞Bcl-2和Bax蛋白表达水平的影响。结果PL抑制MDA-MB-231细胞增殖,并呈现出浓度、时间依赖性。PL诱导MDA-MB-231细胞凋亡,而乙酰-L-半胱氨酸则抑制了PL诱导的细胞凋亡。Western blot结果提示随着PL浓度的增加,Bax的表达逐渐增加,而Bcl-2的表达逐渐减少。结论 PL对人乳腺癌MDA-MB-231细胞有明显的生长抑制和诱导凋亡作用,其机制可能与Bcl-2蛋白表达的减少和Bax蛋白表达增加有关。

Objective The aim of the study was to investigate the effect of piplartine on the proliferation and apoptosis in human breast cancer MDA‐MB‐231 cells line and the mechanism involved .Methods Human breast cancer MDA‐MB‐231 cells line was cultured in vitro .The inhibitory effect of piplartine on the proliferation of MDA‐MB‐231 cells was measured by CCK‐8 assay after treatment with piplartine at different concentrations .The apoptosis rates were measured by flow cytometry .Western blot was used to explore the effect of piplartine on MDA‐MB‐231 cells Bcl‐2 and Bax protein expression .Results The CCK‐8 assay showed that piplartine had an inhibiting effect on the proliferation of MDA‐MB‐231 cells in a concentration and time dependent manner .Piplar‐tine induced apoptosis of MDA‐MB‐231 cells obviously .The antioxidant N‐acetyl‐L‐cystein inhibited the apoptosis of cells .By Western blot analysis ,we found the expression of Bax was up‐regulated whereas that of Bcl