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双语推荐：荧光特性

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本文通过外加电场改变氧化石墨烯团簇分子的共振能量，利用激光激发氧化石墨烯产生的共振荧光特性测量氧化石墨烯在电场作用下的极化动力学特性。发现存在外加电场使得荧光共振峰的半高全宽趋于饱和的时间特性，而不同的氧化石墨烯团簇分子的荧光共振峰的暂态特性同时反映了电场对氧化石墨烯产生定向极化和变形极化的动力学特性。
We have changed the resonance energy of the graphene oxide molecular clusters by using the external electric field. From the graphene oxide resonance fluorescence we could study the polarization dynamics of graphene oxide under the electric field. It is found that the electric field makes the FWHM of fluorescence resonance peak tend to be saturated, and the temporal change of fluorescence resonance of the graphene oxide molecular clusters reflects the directional polarization and the deformation polarization generated in the graphene oxide due to the electric field.

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通过分析HyJet V磷酸酯液压油的环境(润滑油等)及干扰因素(酸度和温度等)的影响,研究荧光法检测飞机用液压油泄漏的可行性。利用荧光分光光度计,获得不同酸度和温度时HyJet V磷酸酯液压油、Jet Oil Ⅱ润滑油、2197润滑油的荧光特性,实验结果:HyJet V磷酸酯液压油、Jet Oil Ⅱ润滑油、2197润滑油的荧光峰分别在362,405和456nm。温度对HyJet V磷酸酯液压油影响较小;Jet Oil Ⅱ润滑油、2197润滑油荧光强度随温度升高逐渐减小。酸度增加时,HyJet V磷酸酯液压油荧光峰从370nm渐变为362nm,荧光强度逐渐减小;Jet Oil Ⅱ润滑油荧光峰保持405nm不变,荧光强度逐渐减小;2197润滑油荧光峰由456nm处红移至523nm,出现双荧光峰,直至荧光特性消失。结果表明:在环境及干扰因素的影响下,HyJet V磷酸酯液压油的荧光特性基本保持不变,并且区别于Jet Oil Ⅱ润滑油、2197润滑油。因此,实验证明利用荧光法检测HyJet V磷酸酯液压油泄漏是可行的。
By analyzing HyJet V phosphate ester hydraulic oil environmental impacts (oil ,etc .) and confounding factors (pH , temperature ,etc .) ,the feasibility was studied for the fluorescence detection of aircraft hydraulic oil leaks .By using the fluores-cence spectrophotometer at various acidities and temperatures ,the fluorescence properties of HyJet V phosphate ester hydraulic oil ,Jet Oil II lubricant and 2197 lubricant were gained .The experimental results are shown as following :The fluorescence peaks of HyJet V phosphate ester hydraulic oil ,Jet Oil II lubricant and 2197 lubricant are at 362 ,405 and 456 nm ,respectively .The impact of temperature on HyJet V phosphate ester hydraulic oil is less effective ;Jet Oil II lubricant and 2197 lubricant fluores-cence intensity decreases with increasing temperature .When acidity increases ,the fluorescence peak of HyJet V phosphate ester hydraulic oil gradient shifts from 370 to 362 nm ,and the fluorescence intensity decreases ;the

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本文在研究了离体人体鼻咽正常组织和癌变组织的荧光寿命的基础上,实验研究了生理盐水的浓度、组织光学特性参数、激发光源的偏振性对癌变和正常鼻咽组织的荧光寿命的影响。实验结果表明:组织的光学特性参数对组织的荧光寿命有不同程度的影响;而不同浓度的生理盐水和光源的偏振性对组织的荧光寿命没有显著的影响。荧光寿命与该发射荧光的强度没有关系,只决定于局部环境,受微环境的物理化学性质因素的影响,因此荧光寿命作为人体组织癌变的检测方法,有着很好的应用前景。
The life-times of normal nasopharyngeal tissues and nasopharyngeal carcinoma tissues were determined.Fur-thermore,impact including concentration of brine,optical tissue properties,polarization of excitation light source on au-to-fluorescence lifetime of human nasopharyngeal carcinoma and normal nasopharyngeal tissues in vitro were investigated, respectively.The results show that optical tissue properties have different effects on fluorescence lifetimes of nasopharyn-geal tissues,while concentration of brine and polarization of excitation light source have no significant influence on fluo-rescence lifetime.Fluorescence lifetime is determined by physical and chemical properties of microenvironment and has nothing to do with fluorescence intensity,it can be used as a testing method for tumor.

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通过荧光光谱仪，确定可乐的弱荧光物质和滤光特性，对532 nm激光激发的红移现象进行解释。自组微调夹持转向装置，利用教学用光栅单色仪定量研究了激光与可乐作用深度对光谱的影响。实验结合了荧光光谱和物质滤光特性等知识，物理内涵丰富，可通过自组实验装置锻炼学生解决实际问题的能力。
Using fluorescence spectrometers ,the weak‐fluorescence substance and the optical fil‐tering properties of Coca‐Cola were found out to explain the red shift phenomenon when excited at 532 nm .The quantitative relationship between excitation depth and spectrum was studied using a set of apparatus and a grating spectrometer .This experiment applied many physics knowledge ,including fluorescence spectrum ,filtering properties etc .A part of the experiment devices needed to be made by students themselves ,w hich could develop their abilities to solve problems in research .

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荧光量子点由于具有发射荧光效率高、光稳定性好、无光漂白等优良特性,因此在生物学研究中得到广泛应用。用量子点标记细胞,通过量子点荧光可以对细胞进行长时程观察,实现活细胞动态示踪。利用量子点和DiO荧光染料对A549细胞膜双染,直观展示了量子点优于有机荧光染料的抗光漂白特性,并阐述了一种利用量子点对细胞A549进行标记并观测在α粒子辐照下细胞损伤动态变化过程的方法,这种方法对于阐明粒子辐射作用细胞的过程、了解辐射损伤的机理有一定的优势。
Owing to the excellent optical properties such as high quantum yield of emission, photo-stability and anti-photobleaching, fluorescent quantum dots(QDs) now have been extensively applied in bioscience re-search. Labeling cells by quantum dots allow long-term observation of the cellular processes. In this paper, we employed both QDs and DiO dye to label cells and thus showed the superior anti-photobleaching property of QDs directly, and also reported the method of application of QDs in observing the damage process of A549 cells induced byα particle irradiation, demonstrating the advantage of application of QDs in the radiobiology research.

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影响量子点荧光特性除了量子点尺寸因素外，其溶液浓度也会对其荧光特性起很重要的作用。但到目前，还未见到有关量子点 CdSe／ZnS 在溶液中浓度对其荧光影响的详细报道。为了掌握影响量子点CdSe／ZnS荧光特性的因素及其物理机制，利用紫外-可见吸收光谱仪和荧光光谱仪测量了尺寸为4 nm的量子点CdSe／ZnS在氯仿中不同浓度下的吸收光谱和荧光光谱，并侧重研究了量子点CdSe／ZnS的浓度对其发光特性的影响并分析了其物理机制。结果表明量子点对光谱的吸收随其浓度的增加而增大，但其发光却具有一个最佳浓度2μmol·L－1。当量子点的浓度＞2μmol·L－1时，量子点的荧光强度随着量子点浓度的增加而降低，而当量子点的浓度＜2μmol·L－1时，量子点的发光强度则随其浓度的降低而降低。其原因主要有两个：（1）荧光猝灭效应；（2）荧光发射与光吸收的竞争效应。当量子点的浓度＞2μmol·L－1时，由于量子点之间相距太近（仅为94 nm）而引起了荧光猝灭效应，且其荧光猝灭效应随量子点间距的减小而增大，且因为吸收过大，导致受激的量子点并未增加，因此，其荧光随浓度的增加而降低。而当量子点的浓度＜2μmol·L－1时，因为量子点之间的距离足够大，不再引起荧光猝灭效应，其荧光强度取决于单位体积内的量子点的个数，单位体积内量子点的个数越多，其发光强度越强。因此其发光强度随量子点溶液的浓度降低而降低。
The authors measured the absorption and the fluorescence spectra of the quantum dots CdSe/ZnS with 4 nm in size at different concentration with the use of the UV-Vis absorption spectroscopy and fluorescence spectrometer.The effect of quantum dots CdSe/ZnS’s concentration on its fluorescence was especially studied and its physical mechanism was analyzed.It was ob-served that the optimal concentration of the quantum dots CdSe/ZnS for fluorescence is 2μmol·L-1 .When the quantum dot’s concentration is over 2μmol·L-1 ,the fluorescence is decreased with the increase in the concentration.While the quantum dot’ s concentration is less than 2μmol·L-1 ,the fluorescence is decreased with the decrease in the concentration.There are two main reasons:1)fluorescence quenching and 2)the competition between absorption and fluorescence.When the quantum dot’s concentration is over 2μmol·L-1 ,the distance between quantum dots is so close that the fluorescence quenching is induced. Th

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目的 利用水溶性ZnSe:Mn/ZnSe掺杂量子点优良的光学特性实现对肝癌细胞株HCCLM9甲胎蛋白(AFP)抗原的特异性免疫荧光标记成像.方法 用巯基丙酸修饰的ZnSe∶Mn/ZnSe掺杂量子点,制备水溶性ZnSe∶Mn/ZnSe-IgG复合物探针.荧光、紫外光光谱分析及透射电镜研究其特性.通过间接免疫荧光法,用水溶性ZnSe∶Mn/ZnSe-IgG复合物探针标记肝癌细胞株HCCLM9 AFP抗原.噻唑蓝(MTT)法检测该复合物探针的细胞毒性,并同传统量子点探针比较.双光子共聚焦激光显微镜观察成像,并进行连续激发实验,同传统量子点探针及有机荧光探针比较光稳定性.结果 该水溶性ZnSe∶Mn/ZnSe-IgG复合物探针具有激发光谱宽、荧光强度高的特点,生物相容性较传统量子点好,无明显细胞毒性.用该探针对肝癌细胞成像清晰,其荧光在共聚焦激光连续激发情况下无明显衰减.结论 ZnSe∶Mn/ZnSe掺杂量子点是一种较理想的荧光材料,比传统量子点毒性更小,并且比传统的有机荧光染料具有更优良的光学特性.
Objective Special immune fluorescent imaging of human hepatocellular carcinoma cell line HCCLM9 AFP antigen with water soluble ZnSe:Mn/ZnSe doped-quantum dots modified by 3-Mercaptopropionic acid.Methods ZnSe:Mn/ZnSe doped-quantum dots were modified with 3-Mercaptopropionic acid to prepare water soluble ZnSe:Mn/ZnSe-IgG probes.The characters of these probes were studied through spectra analyses and transmission electron microscope.These probes were used for special immune fluorescent imaging of human hepatocellular carcinoma cell line HCCLM9 alpha fetal protein (AFP) antigen.Methyl thiazol tetrazolium (MTT) assay were used to compare the cytotoxicity between ZnSe:Mn/ ZnSe-IgG and CdSe/ZnSe-IgG probes.Images were obtained through two-photon confocal laser microscope.Photostability of ZnSe:Mn/ZnSe-IgG probes were studied under continuous illumination by a high intensity laser,comparing with the CdSe/ZnSe and organic fluorescence probes.Results These water soluble ZnSe:Mn/ZnSe-IgG probes

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采用共沉淀合成法,制备了纳米MgO:Eu3+发光材料.通过X射线衍射(XRD)、激发光谱和荧光发射光谱等手段,对所合成样品的相结构、发光特性等进行了表征,探讨了激发光波长对样品光致发光性能的影响.XRD表征结果显示,所合成的纳米MgO:Eu3+材料具有立方晶相结构,颗粒平均尺寸在50nm～60 nm.通过激发光谱和荧光发射光谱的测量,研究了纳米MgO:Eu3+发光材料在不同发射波长下的激发谱特性,以及不同激发波长激发时,样品的荧光发射特性,讨论了激发波长对MgO:Eu3+样品发光特性的影响.
A luminescent material of MgO：Eu3 ＋ was synthesized by conventional co - precipitation method. The structure of the prepared samples was characterized by X - ray diffraction, and the influences of the excitation wavelengths on the properties of photoluminescence were also discussed. The characterization from X - ray diffraction showed that the prepared samples have cubic crystalline phase of magnesium oxide and that the average sizes of the particles are ranging from 50 nm to 60 nm. The luminescence property of MgO：Eu3＋ nano - material under different excitation wavelength was investigated by the measurement of emission spec- tra. And the excitation spectra were also discussed through monitoring the different emission wavelengths. Additionally, the influence of the excitation wavelengths on the spectroscopic properties was studied.

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采用红外光谱、热重分析、紫外光谱和荧光光谱对侧链含查尔酮基团的聚(4-甲基丙烯酰氧基-4''-二甲氨基查尔酮)(PMADMAC)和聚(4-丙烯酰氧基查尔酮)(PAC)的光交联特性进行了研究。随着光照时间的增加,PMADMAC和PAC聚合物光致环加成反应迅速进行,波长短的紫外线更易使得聚合物发生[2+2]环加成反应。与溶液状态相比,固体薄膜状态下的光交联反应速率较慢。PMADMAC聚合物更容易发生光致环加成反应,其光交联速率要比PAC聚合物快,环加成反应也更彻底。采用荧光光谱研究了聚合物的发光特性,发现PAC聚合物无荧光,而PMADMAC聚合物具有溶剂极性敏感的荧光特性。在PMADMAC聚合物的稀溶液中,随着365 nm紫外光照时间的增加,荧光强度迅速降低,其荧光特征波长在紫外光照射后发生蓝移。PMADMAC和PAC聚合物的热稳定性较好,光交联后形成热不稳定的环丁烷结构,其热稳定性有所降低。
The photocrosslinking properties of poly ( 4-methacryloyloxy-4’-dimethyl aminochalcone ) ( PMADMAC) and poly(4-acryloyloxychalcone) ( PAC) homopolymers were investigated by Fourier transform infrared spectoscopy ( FT-IR) , thermogravimetric analysis, UV-visible spectroscopy ( UV-Vis) , and fluorescence spectroscopy. Photocrosslinking results of PMADMAC and PAC indicate the photo-induced [2+2] cycloaddition reaction takes place rapidly with the increase of light irradiation time and is carried out easily at presence of shortwave UV light. The photocrosslinking rate of poly-mers in the state of thin film is slower than that of solution due to the limited molecular motion in sol-id. Compared to PAC, the cycloaddition reaction of PMADMAC with intramolecular charge transfer properties takes place more easily and completely, and the photocrosslinking rate more rapidly. The photoluminescent properties were investigated by fluorescence spectroscopic techniques and the re-sults show

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采用荧光分光光度法测定甲苯咪唑,对甲苯咪唑的荧光特性进行了研究.研究发现,该物质在pH=10的碱性介质中有很强的荧光,其最大激发波长和发射波长分别为302.4nm、361.6nm,甲苯咪唑浓度在0.01~6mg/L范围内与荧光强度呈良好的线性关系.该方法简单、快捷、灵敏度高。
This paper reports the assay of mebendazole by fluorescence spectrophotometry .Fluorescence characteristics of mebendazole have been studied .In the medium of pH=10 , mebendazole has strong flu-orimetric characteristics at 361.6nm.And its fluorescence intensity and the toluene imidazole density as-sume the linear relationship .The method is simple , fast and sensitive .

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