眼底自发荧光是一种非侵入性的眼底成像技术,其可以反映视网膜色素上皮细胞的功能和感光细胞的状态,评价视网膜色素上皮细胞的代谢状况.动态观察眼底自发荧光,有助于对某些与视网膜色素上皮细胞代谢相关的疾病进行诊断、治疗、评估预后及病程随访.文中就眼底自发荧光的机制、种类及其在检测视网膜功能方面的研究进展,作一综述.

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目的 观察结晶样视网膜色素变性患者的眼底红外照相、荧光血管造影及视网膜电图检查特点.方法 对已确诊为结晶样视网膜色素变性的8例患者,进行常规眼底检查,眼底红外照相、荧光血管造影检查及视网膜电图检查,部分患者行OCT检查,观察其眼底病变特点.结果 所有患者均可见无数细小闪亮的黄色结晶体散布在后极部及中周部视网膜上,荧光造影显示不同程度的色素上皮萎缩及增殖,脉络膜血管萎缩.视网膜电图显示部分患者两种视细胞功能不同程度降低.结论 结晶样视网膜色素变性特征为结晶样小体散布于后极部甚至中周部视网膜,所有患者均有不同程度的色素上皮改变及脉络膜萎缩,且病变部位远比常规眼底检查所见要广.

Objective To observe the image features of infrared ray (IR),fundus fluorescein angiography (FFA) and electroretinogram (ERG) in patients with crystalline retinitis pigmentosa.Methods Eight patients (16 eyes) with crystalline retinitis pigrnentosa were examined by funduscopy,IR,FFA and ERG.Part of them was tested optical coherence tomography (OCT).Results The most striking features were numerous tiny refractile yellow dots scattered throughout the posterior pole and the mid-periphery retina associated with macular pigment mottling and depigmentation.FFA revealed diffuse retinal pigment epithelial atrophy and pigment accumulation and showed areas of choroidal atrophy.The electroretinogram noted a reduction in the number of both types of photoreceptors in some cases.Conclusions Crystalline retinitis pigmentosa is characterized by the presence of refringent crystals scattered on the posterior pole even the mid-periphery retina,associated with alterations of retinal pigment epith

采用荧光猝灭光谱、紫外-可见吸收光谱研究了桑色素与人血清白蛋白（ HSA）的结合作用。实验表明桑色素对HSA的荧光猝灭属于单一静态猝灭反应，在溶液中以摩尔比1：1牢固结合，各结合反应的平衡常数Kp〉105，结合常数Kb〉104；根据F？rster非辐射能量转移机理，求算HSA与桑色素间距离r为3.81-3.58nm，能量转移效率E为0.18-0.13。并根据结合反应的热力学常数推测了药物与HSA之间的主要作用力类型为疏水作用力和偶极-偶极作用力。

The interaction of Morin with Human Serum Albumin(HSA) was studied by Spectrometry , UV-visible absorption spectrophotometry and fluorescence spectrometry. The result show that the Morin could induce an endogenous fluorscence quenching of HSA ,which was proved to be a process of static quenching by the fitting equations of Stern-Volmer and double logarithm equation. The binding constants were determined to be more than 104.The binding site(n)was about 1.The binding distance was estimated to be 3.81~3.58nm according to F?rster nonradiation energy transfer mechanism.Energy transfer effciency of E were 0.18~0.13J.The thermodynamic parameters suggested that the interaction forces between Morin and HSA were mainly hydrophobic interaction and dipole-dipole interaction.

目的 探讨不同类型视网膜色素变性中黄斑水肿的临床表现,并回顾文献分析其病理机制.方法 回顾分析各类型视网膜色素变性合并黄斑水肿患者38例(76只眼),分析其眼底荧光血管造影及眼后段OCT检查,总结黄斑水肿的临床表现.结果 38例患者中典型性视网膜色素变性患者21例、无色素性视网膜色素变性患者7例、结晶样视网膜色素变性患者6例、静脉旁色素型视网膜变性患者4例.38例患者眼底OCT检查可见不同程度黄斑水肿,黄斑中心厚度增加,中心凹形态消失,视网膜色素上皮层反光增强,11例患者黄斑区可见大小不一的囊腔存在,中央囊腔较大,囊腔内为无反射液性暗区；38例患者合并视网膜水肿,视网膜神经上皮不同程度增厚.结论 认识并重视视网膜色素变性患者的黄斑水肿具有十分重要的临床意义,眼底OCT在检出及随访黄斑水肿方面具有较高价值.

Objective To determine the feature of cystoid macular edema in patients with retinitis pigrnentosa and analyze its pathological mechanism.Methods Changes in OCT images,fluorescein angiography,and best-corrected visual acuity were analyzed in 38 patients with retinitis pigmentosa combined with macular edema.Results There were typical retinitis pigmentosa in 21 cases,retinitis pigrnentosa sine pigmento in 7 cases,Bietti''s crystalline retinopathy in 6 cases,and Pigmented paravenous retinochoroidal atrophy in 4 patients.Macular edema was evident in all cases,and the normal shape of macular fovea disappeared,foveal cysticlike spaces were evident in 11 cases,all patients also shown retinal nerve layer''s edcma.Conclusions Recognize and value macular edema in patients with retinitis pigmentosa is very important and the use of OCT in the detection and follow-up macular edema have a high value.

目的 观察视网膜和视网膜色素上皮联合错构瘤(CHRRPE)患眼的眼底影像特征.方法 临床确诊为CHRRPE的8例患者8只眼纳入研究.其中,男性5例,女性3例;年龄6～21岁,平均年龄14.13岁.均为单眼发病.就诊时最佳矫正视力0.02～0.2.眼压、眼前节正常.所有患眼均行眼底彩色照相、眼底自身荧光(AF)、荧光素眼底血管造影(FFA)及频域光相干断层扫描(SD-OCT)检查.观察患眼的眼底影像检查特征.结果 所有患眼的CHRRPE病灶均位于后极部,呈黄白色隆起,其周围色素沉着,病变部位视网膜血管纡曲以及前膜形成.8只眼中,病变累及黄斑和视盘3只眼,占37.5％;仅累及黄斑5只眼,占62.5％.病灶隆起处及其周围色素沉着区域AF亮度降低7只眼,占87.5％;AF亮度增强部位与病灶表面致密的前膜相对应1只眼,占12.5％.FFA检查发现,所有患眼病变部位及其周围脉络膜背景荧光减弱,视网膜血管因纤维膜收缩、牵引而呈螺旋样纡曲;病灶处视网膜毛细血管荧光素渗漏,病变部位荧光亮度逐渐增强.均未发生视网膜出血、渗出、毛细血管无灌注区、视网膜新生血管及脉络膜新生血管等异常.SD-OCT检查发现,所有患眼病变部位视网膜内层结构不清、增厚,呈强弱不均匀反射信号;视网膜色素上皮(RPE)及光感受器内外节连接(IS/OS)等外层视

Objective To investigate the characteristics of fundus image in patients with combined hamartoma of the retina and retinal pigment epithelium (CHRRPE).Methods Eight eyes of 8 cases with CHRRPE were included in this study.There were 5 males and 3 females,ranging from 6 to 21 years old (mean 14.13 years).In all cases,unilateral eye was affected.The best-corrected visual acuity (BCVA) was 0.02 to 0.2.The intraocular pressure in all eyes was normal.The ocular anterior segment in all cases was alright.For all cases,fundus photography,fundus autofluorescence (AF),fundus fluorescein angiography (FFA) and spectral-domain optical coherence tomography (SD-OCT) were taken.The characteristics of fundus image were analyzed.Results All the CHRRPE lesions located at posterior pole,presenting as yellow-white elevations,surrounded by hyperpigmentation.Retinal vascular tortuosity and proliferation of epiretinal membrane were found in all CHRRPE lesions.In 8 affected eyes,lesions were involved