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双语推荐:菌根

田间栽培的柑橘根毛少甚至无,高度依赖丛枝菌根替代根毛吸收养分,因而柑橘丛枝菌根的研究被广泛关注。本文综述了近20年国内外柑橘丛枝菌根的形态特征、根际丛枝菌根真菌的资源、丛枝菌根的生理和分子研究以及菌根功能的影响因子的最新进展,重点通过植株生长、营养吸收和抗逆性阐明丛枝菌根的生理功能。
Citrus plants possess less or no root hairs in the fields and thus highly depend on arbuscular mycorrhizas to replace the root hair for nutrient uptake from soil .Therefore,arbuscular mycorrhizas in citrus have been widely concerned .This paper summarizes the new advances in the research of morphology ,fungal re-sources in rhizosphere ,physiology and molecular studies of arbuscular my corrhizas in citrus ,and factors impac-ting mycorrhizal functionings .It clarifies the physiological functions of arbuscular mycorrhizas in terms of plant growth ,nutrient uptake and adverse tolerance .

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以云杉外生菌根菌种为试材,比较了各外生菌根菌与云杉形成菌根的能力以及不同菌根感染率,并探讨了外生菌根菌对云杉苗木生长的影响.结果表明:褐粘盖牛杆菌的最佳培养温度是20~25℃,最适 pH 值是6.0;播种时接种外生菌根菌可提高种子的出苗率,并使出苗时间提前.菌根菌能显著提高云杉幼苗的苗高、地径和侧根数,经褐粘盖牛肝菌、微甜乳菇和土生空团菌液体菌剂处理的种子出苗率比对照提高了26.8%、23.9%、22.0%;就各种菌根菌而言,以微甜乳菇菌株的接种效果最好,其次为褐粘盖牛肝菌和土生空团;黑紫红菇和白乳菇对云杉苗促生效果相对较差.
The ectomycorrhizal fungi of Picea asperata were employed to develop the techniques of seedling cultivation.By inoculating ectomycorrhizal fungi into the roots of seedlings,the effects of nine ec-tomycorrhizal fungi on the growth of four spruces and mycorrhiza infection rates by different inoculation methods was studied.The results indicated that the optimum culture temperature was 20~25 ℃,and the optimum pH was 6.0.Inoculating ectomycorrhizal fungi when the seeds were planted increased percentage of germination and made seeds germinate ahead of time.Ectomycorrhizal fungi promoted the growth of the seedlings in the height,root collar diameter and number of lateral root.The germination rate of seeds was increased by 26.8%,23.9%,22.0% after inoculated with Suillus luteus,Lactarius subdulcis and Cenococ-cum geophilum.Among the tested mycorrhizal fungi,L.subdulcis showed the best,followed by S.luteus and C.geophilum,Russula atropurpurea and L.piperatus had a less growth promotin

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随着森林生态系统的正向演替,植物物种多样性、群落结构、生产力以及土壤条件均会发生显著的变化,这些变化对菌根类型和多样性会产生不同程度的影响。为了探讨群落结构和功能的变化对菌根资源可能产生的影响,选择季风常绿阔叶林及其演替系列上的代表性森林生态系统为对象,对菌根化根系、菌根类型和菌根真菌孢子密度进行调查,并结合已有的群落信息和土壤养分状况,分析在森林演替过程中菌根资源的变化情况和可能的影响因素。结果表明:季风常绿阔叶林各演替阶段的森林生态系统中菌根化比例接近70%,但不同演替阶段森林的优势菌根类型存在明显的差异。处于演替初期的马尾松(Pinus massoniana)林以丛枝菌根为主,占菌根总数的78%;演替中期的针阔叶混交林中的外生菌根占有绝对优势,占75%,是丛枝菌根的3倍;演替顶级的季风常绿阔叶林中的外生菌根和丛枝菌根的比例相当。马尾松林的菌根真菌孢子密度最高,每20 g风干土壤中的孢子数量高达2 925个,是针阔叶混交林的2.5倍,季风常绿阔叶林的2倍。演替系列上的森林生态系统的菌根类型的差异与植物物种多样性和群落结构,尤其是林下的灌木、草本层密度存在一定的相关性,同时也受土壤养分状况的影响。马尾松林具有较丰富的草本植物和较高的草本层密度,并且该森林的土壤相对贫瘠,这些条件都有利于丛枝菌根真菌侵染草本植物的根系形成丛枝菌根并产生大量孢子。针阔叶混交林中外生菌根的优势主要受该森林中外生菌根植物在群落组成上的绝对优势影响。季风常绿阔叶林的物种丰富,群落结构复杂,因此该森林呈现了两种类型菌根优势相当的现象。该文的结果表明,随着季风常绿阔叶林演替的进行,菌根资源在类型上会出现较大的分异,而这种变化受植物物种数量、群落结构的影响,与土壤养分状况存在一定的关系,并且不同演替阶段森林生态系统影响菌根组成的因素存在差异。
Plant species diversity, community structure, productivity and edaphic factors varied significantly with forest succession, these variations may influence the diversity of mycorrhizae. To understand how forest structure and function impact on mycorrhizae at ecosystem level, three subtropical forests were chosen along a forest succession series in South China to investigate mycorrhizae, ectomycorrhizae ( ECM ), arbuscular mycorrhizae ( AM ), and mycorrhizal fungal spores in the top soil ( 0-20 cm depth ). Potential influences of forest structure on mycorrhizae were analyzed based on field census together with plant diversity and soil nutrients. The results showed that nearly 70% of fine roots less than 2 mm diameter in the top soil of each forest were colonized by mycorrhizal fungi, but dominate mycorrhizae types varied significantly in the three successional forests. AM accounted for 78% of total mycor-rhizae in the pine forest at early successional stage, ECM contributed to 75% of myc

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菌根桑能与石漠化地区贫瘠、干旱等主要生态障碍相耦合,有利于减轻贫瘠干旱胁迫,成为喀斯特石漠化生态治理的一种新的有效措施。在综述丛枝菌根真菌的结构、生理生态功能的基础上,初步探讨了石漠化恢复过程中菌根桑的可利用性以及在调查桑树根系丛枝菌根真菌多样性、影响丛枝菌根真菌多样性的关键因子、筛选优良抗性菌株、丛枝菌根真菌与喀斯特石漠化地区桑树的共生机制等几个方面开展前期研究工作,旨在从全新的角度探讨菌根桑对喀斯特生境的适应对策以及为岩溶生态系统退化与恢复机理奠定基础,从而保证菌根桑在石漠化地区的可持续发展。
Mycorrhizal mulberry is a plant, whose mulberry root and Arbuscular Myc-orrhizal fungi came into being a symbiotic relationship by a mean of natural or artifi-cial inoculation. Mycorrhizal mulberry coupling with main ecological barriers, such as barren soil drought in rocky desertification area, wil be beneficial to recovering the karst degraded ecosystem by removing these stresses. So, mycorrhizal mulberry is considered a new effective way in ecological control of karst rocky desertification. The research summarized the structures and the characteristic eco-physiological functions of arbuscular mycorrhizal fungi (AMF), and preliminarily discussed its availability and research in karst rocky desertification restoration ,including investiga-tion of AMF diversity, the key affecting factor of AMF species diversity, the sym-biosis mechanism between AMF and mulberry, promoting the development of agri-cultural economy in Karst Rocky Desertification area by mycorrhizal mulberry. The

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为研究丛枝菌根真菌对黄瓜苗期立枯病的防治效果和相关生理生化变化。以黄瓜品种中农16为材料,播种时接种丛枝菌根真菌,对20 d幼苗人工接种立枯丝核菌。结果表明,接种菌根真菌后能够显著提高黄瓜苗期对立枯病的抗性,降低发病率。黄瓜苗期人工接种立枯丝核菌后菌根苗和非菌根苗发病率分别为16.5%和52.0%,非菌根苗发病严重,病情指数为46.7,接菌病情指数为10,较非接菌苗下降了78.5%,接种菌根真菌提高黄瓜抗病性与改变相关酶活性有关,在病害发生前菌根真菌已经与寄主植物互利共生,提前启动了抵抗病害得相关机制,如提高了保护酶活性,促进植株生长,接种菌根真菌后菌根苗地上鲜质量和地上干质量分别比未接菌处理提高33.10%和25.93%。在病害发生过程中菌根真菌与病原菌互相竞争,抑制病菌对寄主的侵害,减轻细胞膜受损害程度。
The cucumber strain Zhongnong 16 were used to screen the effect of arbuscular mycorrhizal fungi on seedling blight of cucumber in solar greenhouse , and related physiological and biochemistry changes were studied . The result indicated that when cucumber seedlings inoculated with mycorrhizal fungi it can significantly enhance the ability of cucumber resistance .After inoculated rhizoctonia solani incidence rates of AM cucumber seedlings and non A-M cucumber seedlings were 16 .5% and 52 .0%.The mean disease index of cucumber seedling inoculated with AMF was 78.5%lower than that of the control respectively ,moreover,there were most obvious the differences between the treatments and the control .Before disease occurrence the sysbiosis of AMF and host plant had formed and the relevant mechanisms of disease resistance had been started .The inoculation of AMF enhanced plant grewth and improved the activity of protect enzyme .During disease occurred AMF and pathogens compete with ea

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以1年生的白梨树幼苗为试材,通过移栽过程中在根系附近添加50、100和150 mL不同剂量的菌根接种剂来研究菌根真菌对白梨树幼苗磷获取的影响。结果表明,菌根真菌添加120 d后,与对照相比,50、100及150 mL处理的定植率分别是对照的17.4、21.8和29.5倍,并且定植率与菌剂的添加量呈现较好的正相关关系;与定植率相反,菌根真菌明显降低了根际土的pH,菌根处理的pH分别是对照的93%、91%和87%。菌根真菌有效地改善了根际土中磷的有效性和叶片的全磷含量,处理间差异显著,两者均在菌根真菌接种后90 d达到最高值。菌根真菌促进了白梨树幼苗的生长,150 mL处理的株高及分枝数在真菌接种后的120 d分别比对照增加24%和47%。综合分析认为,每株添加150 mL的菌根真菌能较大程度地改善白梨树幼苗对磷元素的获取,促进幼苗的生长发育。
The effects of AM fungi on the phosphorus acquisition of one-year-old Chinese white pear seedling were studied by the application of 50 mL, 100 mL and 150 mL arbuscular macorrhiza (AM) fungi agent into surroundings of root system when pear seedlings were transplanted. The results showed that AM fungi significantly increased the colonization rate in the root with the colonization rate of 50 mL, 100 mL and 150 mL being 17.4, 21.8 and 29.5 times more than that of the con-trol, respectively. A positive trend was found between application amount of AM fungi and colonization percentage. Contrary to colonization rate, AM fungi decreased pH in the soil of AM treatments significantly with 96%,92% and 87% of the con-trol. Phosphorus availability in soil and total content in leaves were improved and peak values were occurred at 90 days after AM fungi were reinforced into soil. Compared with the control, AM fungi enhanced the growth of pear seedlings. Especially in 150 mL treatment, plant height a

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为探明马缨杜鹃菌根真菌的种类,采用 rDNA-ITS-PCR分子生物学方法对分离的马缨杜鹃菌根真菌进行鉴定。结果表明:从马缨杜鹃菌根共分离出6种与杜鹃花类菌根真菌 Phialocephala sp (CK007137)、Oidiodendron sp(HM208742.1)、Oidiodendron sp.shylhs04(AY268186.1)、2个不同的 Ep-acris root associated fungus 类菌株和 fungal sp.R38亲缘关系十分密切的真菌。其中,前5种真菌与其他ERM 植物的菌根真菌同源或近缘,fungal sp.R38是 ERM 植物菌根真菌的新类型。
To explore the species of mycorrhizal fungi of R.delavayi,and provide technical reserves for cultivation and application of R.delavayi,the molecular biological method rDNA-ITS-PCR was employed to identify the isolated mycorrhizal fungi of R.delavayi.Results:six kinds of mycorrhizal fungi closely related to ERM were isolated, including Phialocephala sp (CK007137 ), Oidiodendron sp (HM208742.1 ),Oidiodendron sp. shylhs04 (AY268186.1 ), two kinds of different Epacris root associated fungus,which were the relatives of ERM and fungal sp.R38 which was a new type of ERM respectively.

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随着工农业与城市化的迅速发展,土壤污染和退化越来越严重,土壤修复亟不可待。作为重要环境功能生物之一的菌根真菌越来越显示出其高效、低耗、安全修复土壤的应用潜力。特别是近10年来菌根真菌与其他生物联合修复污染土壤的效应倍受关注。本文总结了菌根真菌与植物、菌根真菌与土壤动物、菌根真菌与细菌联合修复污染和退化土壤的效应;探讨了菌根真菌与其他生物联合修复污染和退化土壤的可能机制;旨在为今后进一步研发多种生物联合协同修复污染与退化土壤的绿色技术提供依据和思路。
As the rapid development of industry,agriculture and urbanization,soil degradation and pollu-tion become more and more serious.It is badly needed to remediate those soils with biological technology. Mycorrhizal fungi,one of the important environmental functional living organisms increasingly show their application potential with higher efficiency,low consumption,and security in remediating soil.Especially in recent 10 years,people have taken more attention to mycorrhizal fungi and other biological effects of contaminated soil bioremediation.This article summarizes the effect of the soil pollution and degradation which is the mycorrhizal fungi and plants,mycorrhizal fungi and soil animals,mycorrhizal fungi and bacte-ria j ointed repairing;discusses mycorrhizal fungi and other organisms j oint remediation of contaminated soils and degradation mechanism might;In order to further develop j oint collaboration variety of biological remediation of contaminated soils and degraded gre

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以高油115和正大619为实验材料,分别通过盆栽和田间试验研究接种摩西球囊霉和地表球囊霉对玉米根系形态和根系内源生长素含量的影响。结果表明,玉米形成菌根后根系形态明显改变,根条数显著多于对照,并且接种处理与对照间的差异随菌根侵染率的上升而加大,差异极显著。生长60 d时,有菌根的玉米单株总根长度为788.61 cm,根系重量达8.26 g,均显著高于无菌根的对照。根系生长素含量随菌根侵染率上升而增加,并且显著高于不接种的对照。丛枝菌根真菌侵染可以促使玉米根内生长素含量上升,根条数增多,增加吸收面积,促进玉米生长。
Maize cultivar GY115 and ZD619 were used for experimental materials cultured in pot and field re-spectively.The effects of Glomus mosseae and G. versiforme on root morphology and root endogenous growth hormone content of maize(Zea mays) were studied. The results showed that the morphology of mycorrhizal maize roots signifi-cantly changed, root number increased after mycorrhization. Furthermore, the difference between mycorrhizal and non-mycorrhizal roots increased with the development of mycorrhizal infection rate. When grew 60 days, mycorrhizal maize total root length per plant of 788.61 cm, fresh root weight per plant of 8.26 g, were significantly higher than non-mycorrhizal root. Growth hormone content in roots increased with mycorrhizal infection rate increasing, and the content of IAA in inoculation treatment was higher than control significantly. This study indicated that AM fungi can induce the growth hormone content increased in maize roots, enhance root number, and thereby inc

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采用美味牛肝菌(Boletus edulis,简称Be)、黄色须腹菌(Rhizopogen luteous,简称Rl)及其混合菌剂分别对湿地松苗接种,以小区对比试验设计,观测接种后的湿地松菌根形成率、成活率、生长量及叶绿素含量。结果表明:接种外生菌根真菌可以显著提高湿地松的菌根形成率和成活率,增加松苗叶绿素含量,对松苗具有显著的促生长效应。同时对接种后的湿地松根际土壤微生物数量进行了测定,结果表明:湿地松根际细菌数量显著增多,真菌数量显著减少,放线菌数量明显增多。
The mycorrhizal infection rate, survival rate, growth and chlorophyll content of Pinus elliottii seedlings inocu-lated with Boletus edulis, Rhizopogen luteous and double inoculated with Be and RI were investigated. A plot comparative test was used in the experiment.The results indicated that the ectomycorrhizal fungi could improve the mycorrhizal infec-tion rate, survival rate and increase the content of chlorophyll in Pinus elliottii seedlings. It had a significant growth pro-moting effect on pine seedlings. The quantity of rhizosphere microrganisms in P. elliottii seedlings was also determined in this study. Results showed that the number of rhizosphere bacteria significantly increased, rhizosphere fungi significantly reduced, and the quantity of actinomyces increased obviously. The technology of ectomycorrhizal fungal inoculation pro-vided theoretical basis and practical guidance for the pine afforestation.

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