蛇床子素是中药蛇床子中主要药理活性成分，有雌激素样作用，在中枢神经系统、心血管系统和免疫系统等方面的疾病治疗应用广泛，本文通过查阅相关文献，对蛇床子素的药理作用及相关作用机制作一综述，为蛇床子素的进一步开发利用提供依据。

Osthole is the main pharmacological active ingredient extracted from Cnidium monnieri (L.) Cuss.. It has estrogen-like effects and has been widely used in the treatment of diseases of central nervous system , cardiovascular system and immune system. This article reviewed the latest study progress of the pharmacological activities and the related mechanism of osthole. The research will provide the basis for the further development and utilization of osthole.

目的：探讨蛇床子素（osthole）对人肺癌细胞A549的杀伤作用及其机制。方法将人肺癌细胞A549用不同浓度的蛇床子素治疗后，采用MTT法检测蛇床子素对A549细胞增殖的抑制作用；通过流式细胞术检测A549细胞内DNA的含量测定蛇床子素对A549细胞周期的影响、蛇床子素处理后A549细胞凋亡情况；Western blot检测蛇床子素处理后A549细胞周期相关蛋白p-Cdc2和Cyclin B1及凋亡相关蛋白Bcl-2和Bax的表达。结果蛇床子素对A549细胞增殖的抑制效应呈剂量依赖性，50μM、100μM、150μM的蛇床子素组增殖抑制率分别为（25.4±3.2）%、（40.2±3.7）%、（63.7±4.5）%。蛇床子素对A549细胞的凋亡诱导效应也呈剂量依赖性，50μM、100μM、150μM的蛇床子素组凋亡率分别为（9.4±1.4）%、（17.3±2.5）%、（22.9±3.3）%。蛇床子素处理后A549细胞周期相关蛋白p-Cdc2、Cyclin B1及抗凋亡蛋白Bcl-2表达显著下降，Bax表达显著增高。结论蛇床子素可诱导人肺腺癌细胞进入G2/M阻滞，并引起肿瘤细胞凋亡性死亡。

Objective To explore the effects of Osthole on the proliferation of A549 cells treated with Osthole and the underlying mechanism. Methods A549 cells were treated with various concentrations of Osthole. Cell pro-liferation was measured by using the MTT assay. Cell cycle was evaluated by using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry. The expressions of cell cycle related proteins Cyclin B1, p-Cdc2 and apoptosis related proteins Bcl-2, Bax were evaluated by Western blotting. Results Osthole significant-ly inhibited the proliferation of A549 cells in a dose-dependent manner. The inhibitory rate of A549 cells treated with 50, 100, and 150μmol/L osthole were(25.4±3.2)%, (40.2±3.7)%, and (63.7±4.5)%, respectively. Osthole also increased the apoptosis of A549 cells in a dose-dependent manner. The apoptosis rate of A549 cells treated with 50, 100, and 150μmol/L osthole were(9.4±1.4)%, (17.3±2.5)%, and (22.9±3.3)%, respectively. West

探讨白芥子促透皮吸收的物质基础．以蛇床子为研究对象，采用改良Franz扩散池，以离体Wistar大鼠背部皮肤为透皮模型，考察白芥子中白芥子碱、白芥子苷和黄酮、白芥子多糖、白芥子脂肪油、白芥子挥发油及不同促渗剂对蛇床子中蛇床子素体外经皮渗透的影响．结果表明，白芥子中挥发油和不同促渗剂均能促进蛇床子素经皮吸收，其作用强弱依次为5％薄荷油（17．8429μg·cm-2·h-1）＞5％冰片（17．0042μg·cm-2·h-1）＞5％氮酮（16．6560μg·cm-2·h-1）＞0．5％白芥子挥发油（16．6101μg·cm-2·h-1）．白芥子中挥发油对蛇床子中蛇床子素具有促透皮吸收作用，百分含量以0．5％为最佳．

To investigate the material basis of Semen Sinapis promoting percutaneous absorp-tion, the Cnidium monnieri was taken as the research object .The penetration experiments in vitro were performed using excised dorsum skin of Wistar rats on modified Franz diffusion cells to study the effect of Semen Sinapis alkali , Semen Sinapis glycosides and flavonoids , Semen Sinapis polysaccharide , Semen Sinapis fatty oil , Semen Sinapis volatile oil from Se-men Sinapis and several penetration enhancers on percutaneous permeation of Osthole .Re-sults indicated that the penetration rate of Osthole by the volatile oil from Semen Sinapis and several penetration enhancers was increased in the following order:5%peppermint oil (17. 842 9 μg· cm-2 · h -1 )>5%borneol (17.004 2 μg· cm -2 · h -1 )>5%azone (16.656 0μg· cm-2· h-1)>0.5% volatile oil from Semen Sinapis(16.610 1 μg· cm -2· h-1). The volatile oil from Semen Sinapis can improve the percutaneous absorption of Osthole from Cnidiu

对近年来有关蛇床子素脑缺血保护作用研究的报道进行分析,发现其不仅能抑制脑缺血再灌过程中的炎性反应、减轻脑水肿而保护受损脑组织,还能增加缺血脑组织中抗氧化酶的活性,清除氧自由基而减少再灌过程中自由基带来的损伤。此外蛇床子素还具有良好的抗凝抗血栓作用,该文从抗炎、抗氧化、抗凝、抗凋亡等几个方面综述蛇床子素发挥保护作用的可能机制,以期为进一步研究蛇床子素防治脑缺血的药理作用提供参考。

In this paper,the publication of osthole in protecting brain ischemia were retrieved and summarized. Our analysis found that osthole can not only decrease the cerebral ischemia reperfusion injury by inhibiting the inflammation reaction and lowering cerebral edema,but also increase the activity of antioxidant enzymes in the ischemic tissue of the brain, scavenge oxygen free radicals and reduce ischemia reperfusion injury caused by free radicals. Furthermore,osthole also exhibited a good anticoagulant effect. So the possible mechanisms of the protective role of osthole were reviewed from anti-inflammatory,anti-oxidation, anti-coagulant,anti-apoptotic and other aspects,in order to provide support for the further pharmacological studies of osthole in brain cerebral ischemia.

观察蛇床子素对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠神经元凋亡及细胞周期的影响,探讨蛇床子素的神经保护作用及其作用机制。方法:采用一次性侧脑室注射(icv)聚集态β淀粉样肽(β-amyloid peptide,Aβ25-35)建立AD大鼠模型,腹腔注射(ip)蛇床子素12.5,25.0 mg·kg-1进行干预,观察大鼠认知功能、神经元凋亡及细胞周期变化。结果:蛇床子素能明显改善AD模型大鼠空间学习记忆能力,减少神经元凋亡,增加S期细胞百分率,促进G2/M期细胞进一步分裂,增强细胞增殖活性,调节细胞周期,有利于维持神经元正常的生理功能。结论:蛇床子素可通过减少神经元凋亡、调节细胞周期发挥神经保护作用,这可能是其改善AD大鼠学习记忆障碍的作用机制之一。

Objective:To study the neuroprotective effects and its mechanism of osthole by observing neuronal apoptosis and cell cycle in Alzheimer’s disease(AD)rats. Methods:An intracerebroventricular(icv)injection ofβ-amyloid peptide(Aβ25-35)was administrated to establish AD rat model. Osthole(12.5,25.0 mg·kg-1)was injected intraperitoneally to rats. Cognitive functions,neuronal apoptosis and cell cycle of AD rats were observed. Results:Osthole could improve spatial learning and memory abilities of AD rats,reduce neuronal apoptosis,increase the percentage of S phase cells,promote the G2/M phase cells to divide further,strengthen the cell proliferation activity,regulate cell cycle and benefit to the maintenance of normal physiological function of neurons. Conclusion:Osthole has neuronal protection through reducing neuronal apoptosis and regulating cell cycle,which may be one of mechanism of improving learning and memory disorder in AD rats.