目的：探讨莱菔硫烷联合茶多酚对肺癌组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的表达的影响。方法选取经病理证实并手术治疗的肺癌患者56例，随机分为治疗组和对照组。采用ELISA测定组织中蛋白激酶A锚定蛋白95、细胞周期蛋白 E2的浓度，免疫组化法测定组织中蛋白激酶 A 锚定蛋白95、细胞周期蛋白 E2的表达并统计微血管密度（MVD），Western blotting法检测组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2蛋白表达情况。结果肺癌患者组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的浓度水平高于治疗组（P＜0.05）；肺癌患者癌变组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2的表达均呈阳性，且对照组织中MVD高于治疗组（P＜0.05），Western blotting法检测对照组组织中蛋白激酶A锚定蛋白95、细胞周期蛋白E2表达高于治疗组（P＜0.05）。结论蛋白激酶A锚定蛋白95、细胞周期蛋白E2在肺癌患者组织中均存在表达升高，与肿瘤生长呈正相关，莱菔硫烷联合茶多酚用药对两种蛋白起到抑制作用。

Objective To investigate the effect of sulforaphane combined with tea polyphenols on the expression of protein kinase A anchored protein 95 and cyclin E2 in lung cancer tissue. Methods The 56 cases confirmed by pathology and operation therapy for lung cancer patients were randomly divided into treatment group and control group. The levels of protein kinase A anchored protein 95 and cyclin E2 were determined by ELISA method. Protein A kinase anchoring protein 95, the expression of cyclin E2, and statistics of microvessel density (MVD) were determined by immunohistochemical method. The protein kinase A anchored protein 95 and cyclin E2 protein expression in tissues were detected by Western blotting method. Results The content of protein in the tissues of patients with lung cancer A kinase anchored protein 95 and cyclin E2 was higher than that of combination group, and the difference was statistically significant (P<0.05). The expression of cancerous tissue of lung cancer patien

卵黄蛋白为鱼类早期的胚胎发生和发育提供营养物质并发挥生物功能，鱼类卵黄蛋白原和卵黄脂磷蛋白可作为检测环境中雌激素的生物标志物。系统介绍卵黄蛋白的前体卵黄蛋白原的发生、鱼类卵黄蛋白的主要成分卵黄脂磷蛋白和卵黄高磷蛋白的结构以及鱼类卵黄蛋白生理功能的研究进展，并进行展望。

Yolk proteins serve nutrition and play physiological function during oocyte hydration and embryo- genesis in teleost. Vitellogenin, which is the precursor substance of yolk proteins, has been widely used as an effective and sensitive biomarker for exposure to environmental estrogens in aquatic environments as well as lipovitellin. Recent progresses of the occurrence of vitellogenin, the organization of lipovitellin and phosvitin, and yolk proteins'' physiological function are elaborated. Finally, the developmental prospects of teleost yolk proteins are also discussed.

目的探讨茶多酚联合莱菔硫烷对肺癌组织中蛋白激酶A锚定蛋白95和细胞周期蛋白E2表达的影响。方法选取经病理证实并手术治疗的肺癌患者56例,随机分为联合给药组和对照组,每组28例。采用ELISA测定所有患者肺组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的含量;免疫组化SP法测定组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的表达并统计微血管密度(microvascular density,MVD);Western Blot检测组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2蛋白表达情况。结果对照组肺癌患者组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的含量均高于联合给药组,差异有统计学意义(P0.01);免疫组化SP法结果显示肺癌患者癌变组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2的表达均呈阳性表达,且对照组织中MVD高于联合给药组(P0.01);Western Blot结果显示对照组组织中蛋白激酶A锚定蛋白95及细胞周期蛋白E2蛋白表达高于联合给药组(P0.01)。结论蛋白激酶A锚定蛋白95及细胞周期蛋白E2在肺癌患者组织中均存在表达升高现象,2者表达呈正相关,与药物治疗呈负相关,可以作为肺癌的评估检测指标。

Objective To investigate the effect of tea polyphenols and sulforaphane on the expression of protein kinase A anchoring protein 95 and cyclin E2 in lung cancer tissue.Methods 56 cases confirmed by pathology and operation therapy for lung cancer patients,were randomly divided into combined treatment group and control group,each had 28 cases.The content of protein kinase A anchoring protein 95 and cell cycle protein E2 in all patients were determined with ELISA.The expression of protein kinase A anchoring protein 95,cyclin E2 and statistics of microvessel density(MVD) were detected by immunohistochemical SP method.The protein levels of protein kinase A anchoring protein 95 and cell cycle protein E2 protein were detected by Western Blot. Results The contents of protein kinase A anchoring protein 95 and cyclin E2 in control group were higher than that in combination group,the differences were statistically significant(P<0.01 ).Immunohistochemical SP results showed that protein kinase A anc

目的优化小鼠主动脉平滑肌细胞膜蛋白提取方法,获得高纯度的Robo4膜蛋白。方法高速离心法选择性地分离提取总膜蛋白,BCA法测定总膜蛋白含量,Western-Blot检测目的蛋白条带。结果成功提取总膜蛋白,总膜蛋白浓度达1.47μg/μL,Western-Blot检测出Robo4膜蛋白条带。结论该提取方法简单、可靠、快速,获得的膜蛋白纯度高,可用于Western-Blot后续试验。

Objective To optimize the method of extracting membrane proteins in mouse aortic smooth muscle cells and harvest high-purity Robo4 membrane proteins .Methods The total membrane proteins were selectively ex-tracted by high speed centrifuge ,the total membrane protein concentrations were measured by BCA ,and Robo4 mem-brane proteins were assessed by Western-Blot .Results The total membrane proteins were successfully extracted ,the membrane protein concentration was 1 .47μg/μL ,and target protein bands were detected by western blot successful-ly .Conclusion This extraction method was simple ,reliable ,fast ,and could harvest high purity of membrane pro-teins .It can be used for follow-up testing of western blot .

目的：探讨短期视觉剥夺对视网膜蛋白表达的影响。方法在大鼠出生后14 d，行双眼睑缝合14d，建立视觉剥夺模型。通过二维凝胶电泳分离视网膜蛋白，用PDQuest软件选择差异表达的蛋白，用质谱仪鉴定表达差异的蛋白，并用Western blot和免疫组织化学法进一步确定差异蛋白的变化，用HE染色观察视网膜细胞的变化。结果视觉剥夺后，视网膜中4个蛋白的表达量下降超过（2.5±0.5）倍。它们分别是热休克蛋白70、糖酵解蛋白α-烯醇化酶、转运蛋白血清铁传递蛋白和钙离子结合蛋白恢复蛋白。然而未发现视网膜细胞数量的减少。结论短期视觉剥夺可以引起视网膜中蛋白的变化，可能与弱视的产生有密切关系。

Objective To explore the influence of visual deprivation on retinal protein expression. Methods The visual deprivation model is established by bilateral eyelid closure. Bilateral eyelid closure was performed at the postnatal 14 days, 2 weeks after which the rats were used for further experiment Two-dimensional gel electrophoresis was used to separate proteins in the retina, the differential proteins were chosen using PDQuest software and identified by tandem MS. the change of protein expression was further confirmed by Western blot and immunohistochemistry. Hematoxylin and eosin staining was performed to observe the retinal structure. Results Expressions of four proteins in the rat retina were decreased after visual deprivation. These proteins were identified as heat shock 70、alpha-enolase、serotranfferin and recoverin. However, we did not observe the change of the amoun of the retinal cells under the light microscope. Conclusions Short-term visual deprivation induces the ch