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双语推荐:酸性磷酸酶

在不同温度下培养黑豆,提取不同萌发时期的酸性磷酸酯酶,利用福林-酚法测定其活力,研究温度对黑豆萌发时酸性磷酸酯酶活力的影响。结果表明,在15~35℃的温度范围内,黑豆在萌发时期,酸性磷酸酯酶的活力总体呈现出先上升、达到最大后又略微降低的现象。温度较高时,黑豆萌发较快,酸性磷酸酯酶达到最大酶活力所需时间较短;温度较低时,黑豆萌发过程中的最大酸性磷酸酯酶活力要低于较高温度下萌发的最大酶活力。
To study the effect of temperature on acid phosphatase activity during the period of black soy?bean germination,black soybeans were cultivated under different temperatures,and acid phosphatase from different periods of germination was extracted,then the acid phosphatase enzyme activity was determined us?ing Folin-phenol method.Research shows that,in the temperature range of 15~35℃,acid phosphatase activi?ty during germination first increases,and then slightly decreases after reaching its maximum.Black soybeans germinate rapidly under higher temperature,and acid phosphatase activity reaches maximum in shorter time. Besides,the maximum activity of acid phosphatase from black soybeans cultivated under lower temperature is less than that of higher temperature.

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通过自制根箱试验,研究了植物根际土壤酶活性(脲酶,转化酶,酸性磷酸酶,碱性磷酸酶)和微生物区系(细菌、真菌、放线菌)在不同蕹菜品种中( Cd-PSC,non-Cd-PSC)镉积累行为的特征。结果表明:蕹菜茎叶的镉积累特性具有稳定性。相同条件下,根际土壤的4种酶活性和3类微生物数量都显著大于非根际土壤。在蕹菜两个品种间,3类微生物的数量差异显著,其大小排序为:细菌,真菌,放线菌;转化酶、酸性磷酸酶、碱性磷酸酶在品种间差异极显著,其大小排序为:酸性磷酸酶,转化酶,碱性磷酸酶;脲酶在品种间的差异性不显著。
A root box experiment was conducted to study the characteristics of four kinds of rhizosphere soil enzymes (urease, invertase, and acid and alkaline phosphatases ) and three kinds of microflora (bacteria, fungi, and actinomyces ) in cadmium accumulation of different species of Ipomoea aquatica Forsk.( Cd-PSC, non-Cd-PSC) .The results show that the cadmium accumulation in the stems of leaves of Ipomoea aquatica Forsk.was stable .Under the same conditions , the enzymatic activities and the microorganism amounts were significantly greater in the rhizosphere soil than in the non-rhizosphere soil . There were significant differences in the microorganism amount between the two species of Ipomoea aquatica Forsk., showing a descending order in amount , as follows:bacteria, fungi, and actinomycetes.Significant differences existed in the species of invertase , and in acid and alkaline phosphatases , showing a descending order in amount as follows:acid phosphatase , invertase , and alka

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背景:抗酒石酸酸性磷酸酶是破骨细胞分化及骨吸收功能的特异性标志酶,是破骨细胞分化成熟的标志。 目的:观察双膦酸盐对破骨细胞分化及骨吸收功能相关因子抗酒石酸酸性磷酸酶的影响。 方法:小鼠单核巨噬细胞RAW264.7诱导培养破骨细胞。实验分2组:对照组开始时加入质量浓度100μg/L核因子kB受体活化因子配体进行诱导至收获细胞,双膦酸盐组在对照组的基础上加入10-7 mol/L阿仑膦酸盐处理至收获细胞。培养第7天检测各组破骨细胞生成和骨吸收功能,免疫荧光检测两组抗酒石酸酸性磷酸酶表达的差异,Western blot检测抗酒石酸酸性磷酸酶蛋白表达情况。 结果与结论:各组细胞均有抗酒石酸酸性磷酸酶阳性多核破骨细胞生成,并在牙本质磨片上形成吸收陷窝;但对照组抗酒石酸酸性磷酸酶阳性多核细胞数目、吸收陷窝数目及陷窝面积均大于双膦酸盐组(P<0.01)。免疫荧光检测显示,对照组抗酒石酸酸性磷酸酶表达均强于双膦酸盐组(P<0.01)。Western blot检测显示,双膦酸盐组抗酒石酸酸性磷酸酶蛋白的表达低于对照组(P<0.01)。说明双膦酸盐通过抑制抗酒石酸酸性磷酸酶蛋白的表达,阻碍破骨细胞分化生成及骨吸收功能。
BACKGROUND:Tartrate-resistant acid phosphatase is a specific marker for osteoclast differentiation and bone resorption, which is a sign of osteoclast maturity. OBJECTIVE:To study the effect of alendronate on tartrate-resistant acid phosphatase related to osteoclast differentiation and bone resorption. METHODOsteoclasts were cultured by mouse monocyte-macrophage cellline-RAW264.7. The cells were divided into two groupcontrol group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor;alendronate group, treated with 100μg/L receptor activator of nuclear factorκB ligand factor+10-7 mol/L alendronate. Osteoclastogenesis and resorption function of osteoclasts were examined at 7 days of culture. Gene expression of tartrate-resistant acid phosphatase was detected by immunofluorescence method. Western blot assay was used to detect protein expression of tartrate-resistant acid phosphatase. RESULTS AND CONCLUSION:Tartrate-resistant acid phosphatase positive mul

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鉴于桉树主要栽植区林地有效磷匮乏且分布空间高度异质性,造成了桉树人工林可持续发展严重受阻的现状,本文在构建不同供磷方式的室内盆栽环境的基础上,选择我国南方主要栽培的8个桉树品种为研究对象,通过测定分析不同桉树在不同的磷环境下其叶片和根系酸性磷酸酶活性的差异性规律,为筛选高效利用品种桉树提供依据,以缓解林地磷素匮乏造成桉树人工林生产力下降的困境。结果表明:巨桉9叶片在低磷环境下具有较高的酸性磷酸酶活性,而尾巨桉3216在磷充足的条件下能够充分的吸收外界的磷素,提高自身的酸性磷酸酶活性;桉树根系酸性磷酸酶的活性大小为:邓恩桉在低磷环境下能够提高酸性磷酸酶活性,而在磷充足的条件下,尾巨桉3216能够充分的吸收外界的磷素,提高自身的酸性磷酸酶活性。
Due to the effective-phosphorus shortage of Eucalyptus robusta planting area and distribution of land space high heterogeneity, the sustainable development of artificial Eucalyptus robusta is baffled. Base on indoor plants envi-ronment, different ways of applying phosphorus are adopted. And eight main cultivation Eucalyptus robusta variety of south China are developed as the research object. The law of different phosphatase activity of leaf blade and root sys-tem in different Eucalyptus robusta variety with variational phosphorus environment is analyzed. According to the law, high effective utilization Eucalyptus robusta variety can be developed. Meanwhile the trouble of decreasing in Euca-lyptus robusta planting productivity is solved to some content. The conclusion shows: leaf blade of E. geandis 9 has high acid phosphatase activity in low degress of phosphorus, while E. urophyllaíE. grandis DH-32-16 can fully ab-sorb outside phosphorus in sufficient phosphorus conditon to

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分析不同磷处理下的大豆幼叶、根尖酸性磷酸酶活性与植株磷利用率间的相关性,提供能够快速准确筛选磷高效利用大豆基因型的生化指标;在3种不同磷处理水平下,测定并比较12个大豆品种的幼叶、根尖酸性磷酸酶活性以及植株磷利用率;检测2μmol/L磷处理下的159份大豆品种的根尖酸性磷酸酶活性。2μmol/L磷处理下,根尖酸性磷酸酶活性与磷利用率间存在极显著正相关(rr0.0 5),并从供试171份材料中筛选出6个磷高效大豆基因型;低磷条件下,大豆根尖酸性磷酸酶活性可作为磷高效基因型筛选的重要生化指标。
Study the relationships between the shoot or root acid phosphatase activity (APA) and phosphorus utilization ratio under different phosphorus treatments ,to provide a basis for screening P efficiency varieties in soy -bean.Under three different P supply levels,the soybean shoot and root acid phosphatase activities and P utilization ratio of 12 genotypes were determinated and contrasted .The root acid phosphatase activities of 159 genotypes were also detected under 2 μmol/L P concentration.The results showed that soybean root acid phosphatase activity was positively correlated with P utilization ratio significantly (r >r0.01 )under 2 μmol/L P concentration.Six phosphorus efficiency varieties were screened out from 171 experimental genotypes.Under low phosphorus concentration ,soy-bean root acid phosphatase activity coule be used as an important biochemistry index to screen P efficient genotype .

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研究了玉米不同生长发育时期根际土壤酶活性对宽窄行休耕轮作和常规均匀垄2种种植方式的响应。结果表明:宽窄行种植方式下的土壤脲酶、酸性磷酸酶、中性磷酸酶和转化酶活性显著高于均匀垄种植方式,而蛋白酶活性二者差异不显著;不同时期的土壤酶活性也有一定的规律性变化,除土壤脲酶和酸性磷酸酶外,6月27日的土壤酶活性普遍高于8月和10月2个时期。可见玉米宽窄行种植方式具有较高的土壤脲酶、酸性磷酸酶、中性磷酸酶和转化酶活性,更好地为作物生长提供C、N、P的供应。对提高玉米产量具有重要意义。
This paper studies the response to two kinds of planting patterns of narrow-wide row fallow crop rota-tion and conventional uniform row of soil enzyme activities in crop rhizosphere soil at different growth period. The re-sults showed that urease,acid phosphatase,neutral phosphatase and invertase activities of narrow-wide row planting patterns were significantly higher than that of uniform row planting patterns,but the differences in protease activity of these two patterns were not significant. Soil enzyme activities in different period have regular changes. Except ure-ase and acid phosphatase,soil enzyme activities on June 27th are significantly higher than that in August and Octo-ber. Narrow-wide row planting patterns of maize have higher soil urease,acid phosphatase,neutral phosphatase and invertase activities,which can better provide C,N,P for corn growing. Consequently,further study on the effect of narrow-wide row planting patterns of maize on soil enzyme activities,will instruct

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采用盆栽土培的方法,研究了不同土壤类型外源添加不同浓度的镉( Cd,0,1.5,3.0,6.0 mg/kg)对烤烟生长发育以及烤烟根际土壤微生物量(细菌、真菌、放线菌)及酶活性(脲酶、转化酶、酸性磷酸酶)的影响。结果表明:在试验的范围内,外源Cd对烤烟生长和土壤微生物数量及酶活性的影响程度与土壤类型及添加Cd浓度有关。随着添加Cd浓度增加,烤烟的生长受到抑制,在添加Cd浓度为6.0 mg/kg时,各部位的干物质降低最多,特别是红壤中烤烟根系受抑制更明显。土类和添加Cd浓度对微生物数量的影响顺序为放线菌>真菌>细菌。土类对酶活性的影响顺序为脲酶>转化酶>酸性磷酸酶,添加Cd浓度对酶活性的影响顺序为酸性磷酸酶>转化酶>脲酶。
Pot experiment was conducted to study the effect of exogenous cadmium on flue-cured tobacco bio-mass and soil biota indexes in different soil .The results showed that there was significantly relation between the dose of exogenous cadmium and tobacco biomass ,microbe quantity and enzyme activity in rhizosphere soil .With the in-creasing of cadmium ,the growth of tobacco was suppressed more seriously ,and under the treatment of 6.0 mg/kg Cd,the dry weight was least ,the inhibition of Cd was more seriously in red soil ,especially.Moreover,it was found that inhibition of cadmium on quantity was in the order of actinomycete >fungi>bacteria ,the effect on enzyme ac-tivity was in the order of urease >invertase>acid phosphatase ,the effect on enzyme activity was in the order of acid phosphatase>invertase>urease.

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目的 观察前列癃闭通胶囊对大鼠前列腺增生的影响,为临床中药治疗前列腺增生提供依据.方法 取SD雄性2个月龄大鼠30只,完全随机分成正常对照组、模型组和前列癃闭通治疗组各10只.应用去势大鼠皮下注射丙酸睾丸酮诱导前列腺增生,观察正常对照组、模型组、前列癃闭通组大鼠前列腺的湿重、前列腺指数、酸性磷酸酶的变化.结果 前列癃闭通组大鼠前列腺的湿重(375 ±58)mg、前列腺指数(1.68±0.22)和酸性磷酸酶(5.6±1.2)均明显低于模型组[湿重:(419±53) mg;前列腺指数:2.11 ±0.19;酸性磷酸酶:(8.2±1.3)U/L],差异有统计学意义(P<0.01),并接近正常组的数值[湿重:(321±20) mg;前列腺指数:1.08±0.12;酸性磷酸酶:(5.1±0.1)U/L].结论 前列癃闭通胶囊可明显抑制大鼠前列腺增生,抑制大鼠血清酸性磷酸酶水平升高,临床上可以利用中药前列癃闭通胶囊治疗良性前列腺增生症.
Objective To observe influence of Top uroschesis tong capsule on prostatic hyperplasia of rats.Methods A total of 30 of 2 month olds male rats were assigned randomly into normal control group (n =10),model group(n =10) and topuroschesis treatment group(n =10).The prostate hyperplasia of castrated rats was induced by subcutaneous testosterone propionate ; the changes of wet weight,prostatic index,acid phosphatase (ACP) of rat prostate in each group were analyzed.Results Wet weight was (375 ± 58) mg; prostate index was 1.68 ± 0.22 and acid phosphatase was 5.6 ± 1.2 in topuroschesis treatment group; wet weight was (419 ± 53) mg; prostate index was 2.11 ± 0.19 ; acid phosphatase was 8.2 ± 1.3 ; there was a significant difference in both groups (P <0 01),and there was no difference between in the topuroschesis treatment group and in the normal control group [wet weight:(321 ± 20) mg; prostate index:1.08 ± 0.12; acid phosphatase:5.1± 0.1].Conclusions Forefront uroschesis

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通过比较不同种源植物的土壤理化性质与生物学活性的差异及根际效应,探讨不同种源植物对庐山酸性山地土壤的环境适应性。在庐山植物园内选择引种多年的11种不同种源的常绿树种,在生长季节中采集根际土和非根际土,测定有机碳含量、碱解氮及有效磷含量、脲酶和酸性磷酸酶活性,并分析各树种根际土壤氮磷养分及酶活性的根际效应。结果表明,(1)不同树种之间的根际土壤酸性磷酸酶(P〈0.01)、有效磷(P〈0.05)和碱解氮(P〈0.01)存在明显差异,而脲酶没有差异。(2)根际与非根际土壤氮磷有效性和酶活性表现出不同的特征,大部分树种根际土壤有效磷及酸性磷酸酶存在一定的根际效应(R/S〉1),例如红花木莲Manglietia insignis、桂南木莲Manglietia chingii及云南拟单性木兰Parakmeria yunnanensis的根际与非根际土壤有效磷含量存在显著差异(P〈0.05),红花木莲、桂南木莲、巴东木莲Manglietia patungensis、红茴香Illicium henryi及红皮糙果茶Camellia crapnelliana与乡土树种云锦杜鹃Rhododendron fortunei根际与非根际土壤酸性磷酸酶存在显著差异(P〈0.05)。但是,根际与非根际土壤碱解氮和脲酶差异不明显,有的甚至出现脲酶的根际负效应(R/S〈1),如巴东木莲和红花木莲等树种根际土壤低于非根际土壤(P〈0.05)。(3)不同种源树种影响下的土壤有效磷和酸性磷酸酶的根际效应(R/S比值)明显,原产西南地区的树种如桂南木莲、云南拟单性木兰和巴东木莲与华南地区的红花木莲、红茴香等土壤有效磷的根际效应(R/S〉1.5)比原产华东亚热带地区的深山含笑Michelia maudiae和云锦杜鹃更明显,相应的酸性磷酸酶的根际效应也较高。土壤脲酶和碱解氮的根际效应却不明显。(4)土壤有机碳与碱解氮、有效磷和酸性磷酸酶存在显著正相关关系(P〈0.01),土壤碱解氮与有效磷(P〈0.01)和酸性磷酸酶(P〈0.05)具显著正相关关系,酸性磷酸酶与pH值(P〈0.05)显著负相关,说明根际土壤有机物质的积累对根际微区的理化环境及根际土壤养分活性具有重要影响。
To assess the environmental adaptability of tree species from different provenances to the acid soils in Lushan mountains, the physicochemical characteristics and biological enzyme activities in the soils beneath different trees and their rhizospheric effects were investigated. 11 tree species introduced from different provenances were selected typically in Lushan botanical garden. Ingrowing seasons, the rhizosphere and non-rhizosphere soils were collected, and the soil pH values, organic carbon contents, available nitrogen and available phosphorus contents, urease and acid phosphatase activities were measured. The results showed that:(1) there were significant differences in the available nitrogen contents (P 1) in the available phosphorus contents and the acid phosphatase activities in between rhizosphere and non-rhizosphere soils beneath most tree species such as Manglietia insignis, Manglietia chingii. The available phosphorus contents were significant differences (P<0.05) in betwe

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实验测定了黄金鲫血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(T -Bil)、直接胆红素(D-Bil)。结果显示:黄金鲫血清中丙二醛含量为(4.68±1.20)nmol/mL ,血清碱性磷酸酶(24.54±0.485)nmol/L ,血清酸性磷酸酶(1.40±0.138)U/L ,血清丙氨酸氨基转移酶(4.36±1.51)U/L ,血清天门冬氨酸氨基转移酶(45.39±29.29)U/L ,血清总胆红素(7.19±3.54)nmol/L ,血清直接胆红素(3.23±1.90) nmol/L ,血清超氧化物歧化酶(82.57±1.69)U/L ,血清过氧化氢酶(10.28±3.62)U/mL。
This experiment through gold crucian carp blood serum superoxide dismutase (SOD) and malondialdehyde (MDA) ,catalase (CAT) ,alkaline phosphatase (AKP) ,acid phosphatase (ACP) ,al‐anine aminotransferase (ALT) ,aspartate aminotransferase (AST) ,total bilirubin (T -Bil) ,direct bilirubin (D -Bli) were measured .As a result ,the statistical analysis ,gold carp serum malondialde‐hyde content is(4 .68 ± 1 .20)nmol/mL ,Serum alkaline phosphatase is (24 .54 ± 0 .485)nmol/L ,Ser‐um acid phosphatase is (1 .40 ± 0 .138)U/L ,Serum alanine aminotransferase is (4 .36 ± 1 .51)U/L ,Se‐rum aspartate aminotransferase is (45 .39 ± 29 .29)U/L ,Serum total bilirubin is (7 .19 ± 3 .54)nmol/L ,Direct bilirubin is (3 .23 ± 1 .90)nmol/L ,Serum superoxide dismutase is(82 .57 ± 1 .69)U/L ,Serum catalase is (10 .28 ± 3 .62)U/mL .

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