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双语推荐：钙离子ATP酶

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通过建立肥大心肌细胞模型,研究钙通道阻滞剂对心肌细胞钙调素依赖性蛋白激酶II(calcium/calmodulin-dependent protein kinase II,CaMKII)的表达及钙离子浓度的变化对钙泵、ATP凋亡信号调节激酶(apoptosis signal-regulation kinase1 ASK1)、细胞活力及细胞凋亡率的影响,深入了解钙离子拮抗剂在心肌细胞肥大过程中的作用。通过早期应用钙离子拮抗剂,可以明显改善CaMKII信号系统的活性,保持心肌细胞内钙稳态,抑制心肌细胞的凋亡。
To establish the animal′s models of hypertrophied cardiomypcytes,to investigate the effect of L-type calcium channel blockers on the expression of calmodulin-dependent protein kinase II(CaMKII),the Ca2+-ATPase,ATP,ASK1,cell viability and apoptotic in myocardial cells.,in order to understand calcium antagonists in the process of myocardial hypertrophy role thoroughly.It can significantly improve CaMKII signal system activity,and keep the myocardial intracellular calcium homeostasis,inhibiting myocardial apoptosis by the early application of calcium antagonists.

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有研究认为,右美托咪定(Dex)可减轻大鼠局部心肌缺血再灌注损伤,但其机制尚未完全阐明[1].细胞内钙超载是诱发心肌缺血再灌注损伤的重要原因,而三磷酸腺苷(ATP)酶在保持细胞内外Na+、K+、Ca2等多种离子平衡,维持细胞钙稳态方面发挥着重要作用.本研究旨在观察Dex后处理对大鼠心肌缺血再灌注时ATP酶活性的影响,探讨其减轻心肌缺血再灌注损伤的机制.
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李氏禾(Leersia hexandra Swartz)是一种多金属富集植物,通过研究其根系吸收铜的途径和方式,有助于解释李氏禾对铜的富集现象,为开发李氏禾修复铜污染土壤的植物修复技术提供科学依据。本文在温室水培条件下,利用钙离子通道抑制剂(LaCl3)和钾离子通道抑制剂(TEA),研究了李氏禾根系铜吸收与钙、钾离子通道的关系;同时,在研究ATP酶抑制剂、解偶联剂和低温作用对铜吸收影响的基础上,探讨了李氏禾根系铜吸收与能量代谢的关系。结果表明,在1 mmol·L-1钙离子通道抑制剂的作用下,李氏禾根对铜的吸收明显被抑制了(p0.05)。处理48 h后,李氏禾根中铜的浓度较对照下降了39.2%。这说明李氏禾根吸收铜与钙离子通道密切相关。在5 mmol·L-1钾离子通道抑制剂的作用下,李氏禾根对铜的吸收与对照没用显著差异。这表明,李氏禾根系对铜的吸收可能不是通过钾离子通道进行的。ATP酶抑制剂钒酸钠(Na3VO4)显著地抑制李氏禾根对铜的吸收(p0.05)。25μmol·L-1和50μmol·L-1 Na3VO4处理48 h后,李氏禾根中铜的浓度较对照分别下降了26.2%和31.0%。由此,推测李氏禾根系对铜的吸收是一个消耗能量的过程。该结果与解偶联剂抑制实验结果相一致。在25μmol·L-1和50μmol·L-1解偶联剂2,4-二硝基苯酚
Leersia hexandra Swartz is a multi-metal accumulating plant. The study on the mechanisms of Cu uptake by roots of L. hexandra will help to understand how plants accumulate copper. Additionally, understanding Cu uptake mechanisms in plants is also valuable for phytoremediation of Cu-contaminated soil by L. hexandra. In the present work, a series of greenhouse hydroponic experiments were carried out to study the relationship of Cu2+uptake with Ca2+and K+channels using a calcium ion channel blocker (LaCl3) and a potassium ion channel blocker (TEA). Effects of ATPase inhibitor, uncoupler, and low temperature on Cu2+uptake by roots of L. hexandra were also studied, respectively. Based on these experiments, Cu uptake mechanisms in L. hexandra were discussed. The results showed that Ca2+channel blocker significantly limited the Cu uptake (p<0.05). After 48 h treatment with 1 mmol·L-1 LaCl3, Cu concentration in the roots was decreased by 39.2%as compared to the control group, which

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目的评估不同转染及培养条件下COS1细胞过表达钙离子ATP酶SERCA1a的量及活性,探讨影响真核细胞目标蛋白质表达量及活性的因素。方法应用常规的全量或者半量DNA、lipofactamin和plus reagent转染COS1细胞,37℃、34℃或31℃培养2至5d。提取微粒体蛋白后定量并测定SERCA1a的ATP酶活性。结果微粒体蛋白量在细胞培养3至4d达到高峰,改变转染及细胞培养条件,未见明显变化;降低细胞培养温度及提高DNA转染用量,可增加SERCA1a表达量;SERCA1a表达量在8μg DNA转染细胞31℃培养3d达到最高,然而降低细胞培养温度后,SERCA1a的ATP酶活性及EP生成量也随之下降;31℃表达的SERCA1a,其ATP酶活性降低比EP生成量减少的幅度更大,并且ATP酶活性及EP生成量随着细胞培养时间延长而增加。结论应用常规的半量转染试剂、全量DNA瞬时转染COS1细胞,37℃培养3-4d可获得最大量具有正常酶活性的SERCA1a蛋白。降低培养温度虽然可以提高外源性SERCA1a的表达,但却可能影响细胞内蛋白质的准确折叠及正常降解。
Obj ective The amount and activities of calcium ATPase SERCA1 a were evaluated to study the effect of different conditions on the expression of exogenous target proteins.Methods COS1 cells were transfected with routine amount or half amount of DNA,lipofectamine and plus reagent,and then cultured at 37 ℃,34 ℃ or 31 ℃ for 2 to 5 days.Microsomal proteins were prepared by ultracentrifuga-tion.SERCA1 a proteins were quantified and used for the determination of ATP hydrolysis and EP (auto-phosphorylated intermediate)formation.Results The amount of microsomal proteins reach the peak in cells cultured for 3 to 4 days and did not change with other conditions.SERCA1 a per mg microsomal pro-teins increased with more DNA transfection and lower temperature.Although ELISA showed the highest concentration of SERCA1a from 8μg DNA transfected cells cultured at 37 ℃ for 3 days,SERCA1a AT-Pase activity and EP formation were reduced whit the decrease in temperature.For SERCA1 a expressed at

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目的 观察氯通道阻断剂5-硝基-2 (3-苯丙胺)苯甲酸(NPPB)和二异硫氰基芪-2,2’-二磺酸(DIDS)对氧化应激诱导的晶状体上皮细胞(HLE-B3)凋亡的影响,初步探讨其抑制细胞凋亡的机制.方法 实验研究.于2010年11月至2011年6月在福建医科大学附属协和医院实验室以晶状体上皮细胞HLE B-3为实验对象,用500 μmol/L叔丁基过氧化氢(t-BHP)诱导细胞氧化应激损伤作为t-BHP处理组,常规培养的细胞作为对照组.在t-BHP存在或不存在下分别加入5、100、200 μmol/L的氯通道阻断剂NPPB或DIDS培养12 h,通过MTT法检测各组细胞存活率;Annexin V-FITC/PI双染法检测细胞凋亡率;AO/EB双荧光染色观察细胞凋亡形态学改变;采用分光光度法检测细胞中丙二醛(MDA)、三磷酸腺苷(ATP)的含量及超氧化物歧化酶(SOD); Fluo-3/AM荧光探针检测细胞内钙离子含量的变化.结果 与空白对照组相比,t-BHP处理组细胞存活率及凋亡率降低、抗氧化酶SOD活性和细胞内ATP含量降低、细胞内MDA及钙离子含量升高,差异比较有统计学意义(P＜0.01);与t-BHP处理组比较,NPPB或DIDS处理组(100、200 μmol/L)皆可使得细胞存活率增高及凋亡率降低,并明显减少氧化应激引起的凋亡形态,增强SOD活性及提高ATP含量,降低细胞内MDA及钙离子含量
Objective To observe the effect of chloride channels in cell damage induced by oxidative stress and to probe into its possible mechanisms.Methods 500 μmol/L tert-butyl hydroperoxide (t-BHP) was used to induce HLE B-3 cells apoptosis,the cells treated by only t-BHP were used as t-BHP-induced group,and the cells cultured by regular method were used as control group,chloride channel blockers (NPPB or DIDS) with the concentrations of 5,100 and 200 μmol/L were added into the medium for 12h respectively and with or without 500 μmol/L t-BHP-induced the cells at the same time to induce the apoptosis in experimental groups.MTT method was used to observe the cell viability.Apoptosis was measured via the Annexin V-FITC and PI staining,the intracellular free Ca2+ concentration was determined by Fluo-3 fluorometry.AO/EB double fluorescent staining was observed under the fluorescent microscope,and the cells were collected respectively after different treatments for measuring MDA level a

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机械通气可导致呼吸机相关的膈肌功能障碍(ventilation induced diaphragm dysfunction,VIDD),其中膈肌失用性萎缩是造成VIDD的主要原因[1].研究结果显示,失用性萎缩状态下肌纤维蛋白主要通过ATP依赖的泛素-蛋白酶体途径(ubiquitin-proteasome pathway-,UPP)降解[1-3].但UPP系统只分解单体的收缩蛋白,不能降解完整肌纤维的骨架蛋白.而钙蛋白酶(calpain)是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,在肌细胞Z线中浓度最高,主要作用是降解细胞骨架蛋白和蛋白激酶等[3].因此,本研究中观察钙蛋白酶抑制剂(calpeptin)对控制通气条件下大鼠膈肌肌纤维结构和肌萎缩的影响,探讨钙蛋白酶对肌萎缩的影响及其机制.
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[目的]综述中药有效组分防治心肌缺血再灌注损伤机制研究进展。[方法]通过查询近5年来的相关文献,综述近年来干预心肌缺血再灌注损伤效果明显的多种中药组分对心肌再灌注损伤作用机制研究概况。[结果]多种中药有效组分可以通过:①增加内源性抗氧化酶或抗氧化物质的活性或/和合成,减少氧化产物的生成,减轻氧化机制造成的再灌注损伤;②通过增加Ca2+Mg2+-ATP酶及Na+K+-ATP酶的活性,调节离子转运,减轻钙离子超载;③减少IL-1、TNF-α等炎症因子释放,减轻炎症反应;④调节细胞凋亡相关分子的表达,抑制心肌细胞凋亡;⑤调节舒血管活性物质前列环素(PGI2)和缩血管活性物质血栓素A2(TXA2)释放,改善内皮功能等途径发挥抗心肌缺血再灌注损伤作用。[结论]中药有效组分可有效防治心肌缺血再灌注损伤,但目前研究存在受试物质量控制缺乏、重复性差、受试物各成分间相互作用机制不明、有效成分不清、药理作用机制研究有待深入等问题,今后研究应考虑中医药作用特点,结合现代医学研究进展,阐明中药在防治心肌缺血再灌注损伤中的物质基础和作用机制。
[Objective] Summarizing the effect of components from traditional Chinese medicine on mechanism in myocardial ischemia reperfusion injury. [Methods]By querying the nearly five years of related literature, summarizing the advances in studies on various traditional Chinese medicine effective components prevention and treatment for myocardial ischemia reperfusion injury.[Results] Traditional Chinese medicine effective components through the same or different ways play a role, for example,(1)antioxidant,(2)reduce calcium overload, (3)reduce inflammation reaction, (4)inhibit myocardial apoptosis , (5)improve endothelial function. [Conclusion] The review provides further research of traditional Chinese medicine in the prevention and treatment of myocardial ischemia reperfusion injury to provide the reference.

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目的 研究哺乳期多溴联苯醚-153(BDE-153)染毒对成年大鼠皮质细胞内钙离子浓度和钙激活相关酶水平的影响,为研究多溴联苯醚的神经发育毒性提供科学依据.方法 40只雄性新生乳鼠按体重和窝别随机分成1、5、10 mg/kg BDE-153组和橄榄油溶剂对照组,每组10只.在出生第10天,染毒组按0.1 ml/10 g体重一次腹腔注射染毒BDE-153,橄榄油溶剂对照组给予同剂量橄榄油.染毒后2个月时处死大鼠,冰皿上迅速分离大脑皮质,用流式细胞仪测定细胞内Ca2+浓度,用比色法测定钙调神经磷酸酶(CaN)、Ca2+,Mg2+-ATP酶活力,实时荧光定量PCR法和蛋白免疫印迹法(Western-blot)检测钙激活蛋白酶calpain-1和calpain-2的基因和蛋白表达.结果 对照组、1、5和10 mg/kg BDE-153组大鼠皮质细胞内Ca2+平均荧光强度分别为10.83、1.48、1.93和0.62,各染毒组细胞内Ca2+浓度均明显低于对照组,差异有统计学意义(P＜0.05).各染毒组CaN酶活力、Ca2+-Mg2+-ATP酶活力、calpain-1基因和蛋白表达量与对照组的差异无统计学意义(P＞0.05).随着BDE-153染毒剂量的增加,BDE-15染毒组calpain-2蛋白的表达量逐渐升高,与对照组[基因:(0.81±0.26),蛋白:(0.15±0.07)]比较,5、10 mg/kg BDE-153组calpain-2的基因[5 mg/kg BDE-153组:1.16±0.52,10 mg/kg BDE-153组:1.32±0.23]及蛋白表达量[5 mg/kgBDE-153组:0.31±0.07,10 mg/kg BDE-153组:0.37±-0.06]明显升高,差异有统计学意义(P＜0.05)；10 mg/kgBDE-153组calpain-2的蛋白表达量(0.37±0.06)明显高于1 mg/kg BDE-153组(0.22±0.07),差异有统计学意义(P＜0.05).结论 Ca2+介导的calpain-2激活可能是BDE-153神经毒性的主要机制之一.
Objective To investigate the effects of polybrominated diphenyl ether-153 (BDE-153) exposure during lactation period on the calcium ion (Ca2+) concentration and calcium-activated enzyme levels in cerebral cortical cells among adult rats and to provide a scientific basis for the study on the developmental neurotoxicity of BDE-153.Methods Forty newborn male rats were randomly and equally divided into four groups according to their body weights and litters:1,5,and 10 mg/kg BDE-153 groups and olive oil solvent control group.On postnatal day 10 (PND 10),the BDE-153 groups were administrated BDE-153 (0.1 ml/10 g body weight) by intraperitoneal injection,while the olive oil solvent control group was given an equal volume of olive oil.Two months later,these rats were decapitated,and the cerebral cortex was separated quickly on an ice-cold dish.The Ca2+ concentration in cerebral cortical cells was measured by flow cytometry.The activities of calcineurin (CaN) and Ca2+-Mg2+-ATP enzyme were deter

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目的：探讨褪黑素（MLT）对常压下慢性间歇性缺氧SD大鼠心肌的保护作用。方法将24只雄性SD大鼠（5周龄）随机分成3组：对照组、实验A组、实验B组。对照组在空气中正常饲养，实验A组和实验B组在常压慢性间歇性低氧箱内，对照组和实验B组灌服生理盐水，实验A组灌服褪黑素。8周后用光电比色法测定血清肌钙蛋白T（cTnT）的浓度和心肌钙离子ATP酶（Ca2＋-ATPase）的活性，取大鼠右心室做病理切片。结果①实验B组与实验A组比较：实验B 组的cTnT明显增加（P＜0．05），心肌组织的Ca2＋-ATPase的活性明显降低（P＜0．05）。病理切片显示，个别心肌细胞核消失，心肌细胞肥大，心肌之间有大量出血；②实验A组与对照组比较：cTnT和Ca2＋-ATPase均无明显差异（P＞0．05）。病理切片显示，心肌细胞之间有少量出血；③实验B 组与对照组比较：实验B组的cTnT明显增加（P＜0．05），心肌组织的Ca2＋-ATPase的活性明显降低（P＜0．05）。病理切片显示，个别心肌细胞核消失，心肌细胞肥大，心肌之间有大量出血。结论褪黑素对慢性间歇性缺氧心肌具有保护作用。
Objective To investigate whether the melatonin (melatonin,MLT)could protect SD rats’cardiac muscu-lar tissues from chronic intermittent hypoxia damage in the atmospheric pressure.Methods The 24 male,SD rats(5 weeks)were randomly divided into three groups:the Control group,the experimental group A and the experimental group B.The control group rats were fed with normally reared in the air,and the experimental group A and B rats were living in the atmospheric pressure and chronic intermittent hypoxia boxes.The control group rats and the experimental group B rats were fed with normal saline solution (NS ),and the experimental group A rats were fed with melatonin (MLT).After 8 weeks,the cTnT capacity and Ca2+-ATPase activity of each rat were detected,and the right ventri-cles were taken for pathological section.Results ①Comparison of the experimental group B and the experimental group A:The cTnT capacity of the experimental group B was increased (P 0.05).There was a small amoun

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将具有光催化潜力的Zn、Ti金属元素引入凹凸棒粘土(ATPs)结构中,采用浸渍法制备了一系列Zn/TiATPs,并用作光催化剂进行盐酸四环素光降解实验。考察了金属离子配比、焙烧温度等对光催化性能的影响。结果表明,Zn/Ti金属摩尔比为3∶1,煅烧400℃时制备的Zn/Ti-ATPs具有良好的光催化性能。在可见光照射120 min条件下,20 mg Zn/Ti-ATPs对20 mg/L盐酸四环素的光催化降解率可达91.29%。
In order to make the attapulgite clay(ATPs)have the photocatalytic performance,and put the photocatalytic potential metal elements Ti and Zn into the ATPs structure. Then prepared a series of Zn /Ti-ATPs by impregnation method and photocatalytic degradation of the tetracycline hydrochloride. The in-fluence of the ratio of metal ions,roasting temperature,etc. on the properties of photocatalytic materials was investigated. The results show that with the Zn / Ti molar ratio of 3: 1,and the roasting temperature of 400 ℃,the Zn / Ti-ATPs has a good photocatalytic performance. The photocatalytic degradation of tetracy-cline hydrochloride can be made as high as up to 91. 29% in 120 min under the irradiation of xenon lamp light.

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