目的：应用基因芯片技术对移位后的弹性软骨进行基因表达差异研究,并分析弹性软骨特征蛋白基因的差异,筛选出与弹性软骨特征相关的关键核心基因。方法：取健康8周龄C57BL/6小鼠,随机分为正常侧耳廓弹性软骨组、对侧耳廓缺损弹性软骨移植组、背部耳廓弹性软骨移植组。取移植后软骨分析移植后弹性软骨的基因表达以及基因表达差异。结果：差异基因pathway分析可见,与氧化应激相关基因NDUFB1,NDUFB2,COX7A1,UQCRQ；与磷酸化相关NDUFA2,NDUFA3,COX7C,NDUFA1,COX17,ATP5H,C OX6C,NDUFB6,COX6B1,COX5B,COL2A1,COL10A1,ALP,Runx2,ZHD-1,CPD；与再生相关NDUFB1,NDUFB2,COX7A1,UQCRQ,ND UFA2,NDUFA3,COX7C,NDUFA1,ATP5H,COX6C,NDUFB6,COX6B1,COX5B,COL2A1,COL10A1,ALP,Runx2,ZHD-1,CPD；与迁移相关基因COL2A1,COL10A1,ALP,Runx2,ZHD-1,CPD,NDUFB1,NDUFB2,COX7A1,UQCRQ,NDUFA2,NDUFA3,COX7C,NDUFA1,AT P5H,COX6C,NDUFB6,COX6B1,COX5B；与核蛋白体相关基因RPL3I,RPL37A,RPS19,RPL39,RPS11,PRS13,RPS25,RPL27,RPS23,RP LP2；与RNA多聚酶相关POLR2I。差异基因共计23个,上调17个,下调6个。结论：通过动物模型筛选出表达差异基因可为进一步干预弹性软骨移位后的转归提供参数和依据,为临床耳再造提供最为生理的支架材料。

Objective:Differentially expressed genes of auricular cartilage cells were detected by gene chip technology, and the characteristics of elastic cartilage special protein gene were also analyzed to find out the key gene with elastic cartilage characteristic. Methods:Healthy 8-week-old C57BL/6 mice were randomly divided into normal side ear elastic cartilage group, contralateral ear defects elastic cartilage transplantation group, back auricular elastic cartilage transplantation group. Transplanted elastic cartilage were collected to detect the differences in gene expression.Results:With difference gene pathway analysis, NDUFB1 NDUFB2, COX7A1, UQCRQ were oxidative stress-related genes, NDUFA2, NDUFA3, COX7C NDUFA1, Cox17, ATP5H, COX6C NDUFB6, COX6B1, COX5B the COL2A1, COL10A1, ALP , Runx2, ZHD-1, CPD were phosphorylation-related genes. NDUFB1, NDUFB2, COX7A1 UQCRQ, NDUFA2 NDUFA3 COX7C, NDUFA1, ATP5H COX6C NDUFB6 COX6B1 COX5B the COL2A1, COL10A1, ALP, Runx2, ZHD-1, and CPD were regenerati

真核翻译起始因子4A（eIF4A）蛋白属于DEAD-box RNA解旋酶家族,具有RNA依赖的ATP酶活性和ATP依赖的RNA解旋酶活性。脊椎动物中已经鉴定出三种eIF4A：eIF4A1、eIF4A2和e IF4A3。除参与翻译起始,eIF4A家族成员在胚胎发育等多种生命过程中也发挥着重要的作用。虽然三种eIF4A在序列上高度相似,但它们的作用不尽相同。eIF4A成员作用的独特性和多样化通常由相互作用的结合蛋白来调节。本文将eIF4A家族成员的最新研究进展,特别是e IF4A成员各自独特的作用作一综述。

The eukaryotic initiation factor 4A ( eIF4A) belongs to the DEAD -box protein family with the activities of RNA -dependent ATPase and ATP -dependent RNA helicase .Three eIF4A proteins, eIF4A1, eIF4A2 and eIF4A3, had been identified from vertebrates .Besides helicase function in translation initiation, the members of eIF4A also play essential roles in many life processes including embryo develop-ment.Although there are highly similar in sequences , the three eIF4A show diverse roles.The specific and diverse functions of eIF4A are usually regulated by interacting partner proteins .In this paper, the recent re-search advances of eIF4A were reviewed, especially the diverse roles of eIF4A family members.

目的：研究雄黄、水飞雄黄、朱砂、氯化汞（HgCl2）对小鼠肝/肠细胞色素P450（cytochrome P450， CYP450）及肠道P-糖蛋白（P-glycoprotein，P-gp）的影响。方法将120只NIH小鼠随机分为纯水组，羧甲基纤维素钠（CMC）对照组，利福平组（40 mg·kg-1），酮康唑组（1.8 mg·kg-1），雄黄高、低剂量组（60，15 mg·kg-1），水飞雄黄高、低剂量组（60，15 mg·kg-1），朱砂高、低剂量组（120，30 mg·kg-1）和HgCl2高、低剂量组（0.2，0.05 mg·kg-1）。小鼠每天灌胃2次，连续5 d。第6天灌胃1 h后摘取肝脏和全段小肠。用超微量ATP酶测试盒测定肠匀浆液中P-gp依赖性ATP酶的活性；用紫外分光光度法测定梯度离心分离的小鼠肝/肠微粒体中Cyp3a的活性；用实时定量PCR （real-time PCR，RT-PCR）法测定肝中Cyp3a11和肠道P-gp基因（Abcb1b） mRNA的表达。结果小鼠肠道P-gp偶联的ATP酶活性测定结果显示，雄黄、水飞雄黄、朱砂和HgCl2的高、低剂量组与CMC对照组比较差异均有统计学意义（P<0.05，P<0.01）；用红霉素和氨基比林为探针药检测肝脏和小肠微粒体Cyp3a酶活性的结果显示，各供试物组的Cyp3a酶活性均显著高于CMC对照组（P<0.05，P<0.01），而RT-PCR结果显示各供试物均能显著诱导Cyp3a11和抑制Abcb1b的mRNA表达。结论雄黄、水飞雄黄、朱砂、HgCl2对小鼠肝脏和肠道中的Cyp3a均具有显著的诱导作用，而对肠道P-gp的转运活性则有显著抑制作用。

Objective To investigate the effects of realgar,levigated realgar,cinnabar and mercuric chloride(HgCl2) on mice hepatic/intestinal cytochrome P450 (CYP450)and intestinal P-glycoprotein(P-gp). Methods One hundred and twenty NIH mice were randomly divided into normal group, sodium carboxymethylcellulose(SCMC) group, rifampicin group (40 mg·kg-1),ketoconazole group (1.8 mg·kg-1),high- and low-dose realgar groups(60,15 mg· kg-1),high- and low-dose levigated realgar groups (60,15 mg·kg-1),high- and low-dose cinnabar groups (120,30 mg·kg-1), and high- and low-dose HgCl2 groups(0.2, 0.05 mg·kg-1). The mice were orally treated with the corresponding medicine twice a day for 5 continuous days. On the experiment day 6, the liver and intestine were quickly isolated one hour after intragastrical administration. The activities of P-gp coupled ATPase in intestinal homogenate were detected by minim ATP enzyme test kit. The activities of Cyp3a in liver/intestine were detected by ultrav

目的：了解上海市骨科外来器械灭菌前清洗效果。方法2011年12月随机选取上海市骨科手术较多的9所医院外来骨科器械和医院自行清洗器械，采用残留血、残留蛋白、ATP和细菌培养计数等4种方法进行监测。结果共监测1405件手术器械，其中1115件外来器械，290件医院自行清洗器械；残留血阳性率外来器械35．7％、医院自行清洗器械4．3％（OR＝12．39）；残留蛋白阳性率外来器械59．1％、医院自行清洗器械32．5％（OR＝3．00）；A T P对数值外来器械（3．19±0．89）、医院自行清洗器械（1．71±0．47）（ P＜0．001）；细菌菌落数均值外来器械为165．69 CFU／cm2、医院自行清洗器械为12．36 CFU／cm2，秩和检验 P＜0．001。结论外来器械灭菌前清洗不到位，残留血、残留蛋白、ATP和细菌菌落数合格率均低于医院自行清洗器械。

OBJECTIVE To understand the cleaning effects of the rented surgical instruments from orthopedics de-partments in Shanghai .METHODS In Dec 2011 ,the rented surgical instruments that were used frequently for sur-geries in the orthopedics departments of 9 hospitals in Shanghai were randomly collected ,meanwhile ,the surgical instruments cleaned by the hospitals themselves were also included ,then the monitoring was performed by using 4 methods ,including the residual blood ,residual protein ,ATP ,and bacterial culture counts .RESULTS A total of 1405 pieces of surgical instruments have been monitored ,including 1115 pieces of rented surgical instruments and 290 hospital-hold instruments .The positive rate of blood residue test of the rented instruments was 35 .7% ,the hospital-hold instruments 4 .3% (OR=12 .39);the positive rate of protein residue of the rented instruments was 59 .1% ,the hospital-hold instruments 32 .5% (OR=3 .00) .The ATP logarithm value of the rented instrumen

采用普通PCR扩增、SHOT-GUN测序、软件拼接首次获得了池蝶蚌(Hyriopsis schlegelii)线粒体基因组全序列。线粒体基因组全长为15939 bp,由13个蛋白质编码基因、22个tRNA基因、2个SrRNA基因和28个长度为1—393 bp的非编码区组成;除ND3-ND5、ND4L、ATP6、ATP8、COX1-COX3、tRNA-D、tRNA-H之外,其他大多数基因在L链编码。池蝶蚌线粒体全基因组序列、蛋白编码基因、tRNA基因、rRNA基因及非编码区的A+T含量分别为60.36%、59.84%、61.7%、60.23%及62.5%,与其他淡水蚌类一致,均表现出A+T偏好性,淡水蚌类线粒体基因组长度的差异主要表现在非编码区长度的差异。池蝶蚌mtDNA的COX2-12SrRNA区域基因排列存在差异,是ND3、tRNAHis、tRNAAla、tRNASer1、tRNASer2、tRNAGlu、ND2、tRNAMet 8个基因发生重组造成。22个tRNA基因都具有典型的三叶草二级结构,tRNA-E与tRNA-W间的非编码区含有一个ORF区,而控制区并未发现。从GenBank上下载的14种双壳纲贝类的mtDNA序列构建的系统进化树,显示池蝶蚌与三角帆蚌亲缘关系最近。研究结果为淡水珍珠蚌线粒体基因重排及进化特征提供理论依据。

The complete mitochondrial genome of Hyriopsis schlegelii was obtained by using polymerase chain reaction (PCR), shot-gun sequencing. The genome contains 15939 base pairs and 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 28 non-coding regions ranged from 1bp to 393bp in size. Most genes were encoded on the L strand, while ND3-ND5, ND4L, ATP6, ATP8, COX1-COX3, tRNA-D, and tRNA-H were encoded on the H strand. The structure and organization of mitochondrial genomes of H. schlegelii and other 7 freshwater mussels were analyzed by using comparative genomics and bioinformatics methods. Results showed that:(i) Strong bias was toward A+T for the genome of H. schlegelii. (ii) The striking mitochondrial genome difference in the size performed on the non-coding regions in all the freshwater mussels. (iii) The gene arrangement of H. schlegelii was identical to that of Hyriopsis cumingii and Lamprotula leai, but was different from that of Cristaria plicata, Lampsilis or