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双语推荐：Chelex

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通过对Chelex 100和Chilete P两种螯合树脂对水中Sr2＋离子的静态和动态分离研究,考察浓度、pH值、体积和流速等条件的影响,设计正交实验,摸索树脂动态吸附和洗脱的工艺路线。结果表明：Chelex 100比Chilete P具有更好的Sr2＋离子吸附效果,静态分离回收率分别为71.48%和51.49%。Chelex 100树脂的动态分离工艺：浓度为250mg·mL-1、pH值为3的Sr2＋离子溶液20mL,以流速为3ml.min-1通过层析柱吸附;8mol.L-1的HNO3溶液以流速为2ml.min-1进行Sr2＋离子的洗脱;获得最终回收率为87.08%。
The study on the application of chelating resin to separation of strontium metal ions was given .The adsorbing concentra-tion,pH value,volume and velocity of flow of Chelex 100 and Chelite P resin from strontium solutions were studied in a batch reac-tor.Otherwise,orthogonal test was designed.The results show that adsorbing capacity of Chelex 100 resin was strong than Chelite P.the recovery of Sr 2+were 71.48%and 51.49%for static state separation.The optimum conditions of Chelex 100 resin to separation of strontium ions are as following , absorption conditions:concentration 250mg· mL-1 , pH 3, volume 20mL, velocity of flow 2ml.min-1;desorption conditions:HNO3 concentration 8mol· L-1,velocity of flow 2ml· min-1.Ultimately,the recovery of Sr2+was 87.08%.

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对Chelex-100树脂对铜和钠的吸附系数Kd进行了测定，研究pH对吸附效果的影响，分析铜和钠的柱行为，并绘制了淋洗曲线，测定Chelex-100树脂对铅、镉、铜的吸附容量。使用自制小型离子交换柱，建立Chelex-100树脂分离-石墨炉原子吸收法测定养殖用海水中铜的方法，成功将铜与氯化钠基体分离，消除了基体干扰。方法回收率：94%～102%， RSD：1.5%～4.2%。
Separation method using Chelex-100 chelating resin and GFAAS determination of copper in seawater for culture was eatablished. This method could solve the problem of interference on the determination of copper from sodium chloride matrix. Kd value of Na and Cu on chelating resin was detected. The influence of pH value of solution on adsorption and behavior of ion on resin were analyzed. Elution curve for Na and Cu was drawn. Adsorption capacity of resin was detected. Recovery of method:94%-102%, RSD:1.5%-4.2%.

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对昆虫病原体的直接取样、检测及定量化能够直接反映昆虫流行病学调查中的病原体丰度。本文以在田间蚜群中广泛流行的虫霉目真菌新蚜虫疠霉为研究对象,通过对不同来源的新蚜虫疠霉样品的DNA提取测试,建立Lysis提取结合Chelex-100方法的PCR检测技术体系,并以此方法为支撑进行了田间麦蚜新蚜虫疠霉流行病的调查验证。结果表明改良的Chelex方法不仅为昆虫病原真菌的田间流行学调查提供了一个快速、准确的评价方法,而且在其他真菌的核酸快速、痕量检测方面也可能具有重要的应用潜力。
Direct sampling, detection and quantification of entomopathogens can directly reflect the epizootiology of pest in abundance. Pandora neoaphidis, a widespread obligated-aphid pathogen, was investigated in aphid populations. A PCR technology system based on modified Lysis extraction associated with Chelex-100 was developed for epizootiological investigation of entomophthoralean fungi on wheat aphid. Results show that the developed method not only provide a rapid and accurate tool for epizootiological investigation of entomopathogenic fungus, but also have a potential application for rapid and tiny-nucleic acid detection in other fungus.

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目的：对5种DNA提取方法进行比对，为选择适用于痰中结核分枝杆菌DNA的提取方法提供依据。方法：采用PureLink genomic DNA kits (PLKm)、苯酚与氯仿法(PCm)、Chelex-100法(Cm)、磷酸盐缓冲液法(PBm)及硅珠法(SBm)5种方法提取痰标本中的DNA，对提取产物的浓度、纯度等技术指标进行分析，并对核酸扩增结果进行统计。结果：DNA浓度均值由高到低依次是PBm、Cm、PCm、SBm、PLKm，组间比较差异有统计学意义，两两比较发现PLKm与PCm、Cm、PBm之间差异有统计学意义，但Cm与SBm间的比较有统计学意义；5种提取方法的OD260：OD280比值均值与1.8的接近程度依次是PLKm、PCm、Cm、PBm、SBm，组间比较差异有统计学意义，两两比较发现除SBm与另外4种方法间存的差异具有统计学意义外，其余各组间的比较均无统计学意义；采用PLKm、PCm及Cm提取的产物均扩增出阳性结果，而PBm、SBm提取产物分别有18例和11例未获得阳性结果，经Mc-Nemar x2检验方法间的差异有统计学意义。结论：Chelex-100提取痰标本中结核分枝杆菌DNA是一种简便、省时、廉价适用于基层结核病实验室进行核酸检测的方法。
Objective Comparison five DNA extraction methods of sputum specimen, To select the applicable DNA extraction method of Mycobacterium tuberculosis in basic tuberculosis research laboratories. Methods Using the five methods to extract DNA of Mycobacterium tuberculosis from sputum, PureLink genomic DNA kits (PLKm), phenol and chloroform method (PCm), Chelex-100 method (Cm), Phosphate buffer method (PBm) and silica beads method (SBm), to analyze concentration, purity and other technical indicators of the extraction product, and to do the statistical analysis of nucleic acid amplification results. Results The Average DNA concentration values from high to low in turn is PBm, Cm, PCm, SBm and PLKm, there was statistically significant difference between the groups.The Average values of OD260:OD280 Proximity to 1.8 from high to low in turn is PLKm, PCm, Cm, PBm and SBm, there was statistically significant dif-ference between the groups(P=0.001),there were statistically significant difference be

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研究了一种测定盐湖水中铀的含量及234 U/238 U活度比值的方法。采用Chelex-100螯合树脂将盐湖水中大量的可溶盐基体与铀分离,钠的消除率大于99.9%,铀的回收率为90.5%~106%,4次平行测定结果的相对标准偏差小于10%。经P350树脂进一步纯化后,由α能谱仪测定234 U/238 U活度比值,相对标准偏差小于5%(n=3)。研究结果表明,该基体消除法可以用于盐湖水中铀的含量及234 U/238 U活度比值的测定。该技术已被应用于实际生产中。
A matrix pre-separation method for the determination of uranium content and 234 U/238 U activity ratios in saline waters by ICP-MS andα-spectrometry was studied.The uranium was well pre-separated from saline waters by Chelex-100 resin.The separation efficiency of Na is higher than 99.9%.The recovery of uranium is in the range of 90.5%-106% with sr<10% (n=4).The 234 U/238 U activity ratios were achieved byα-spectrometry after removing the interferences by P350 resin.The results show that the matrix pre-removal method can be well adapted to determine 234 U/238 U activity ratios in saline waters.The sr of 3 times determination is less than 5%.The developed method has been already applied to determine the uranium content and 234 U/238 U activity ratios in real saline waters.

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目的对双亲缺失的姐妹进行同胞鉴定。方法采用chelex-100法提取血液DNA,通过检测常染色体短串联重复序列(short tandem repeat,STR)位点、X染色体STR位点和线粒体单核苷酸多态性(single nucleotide polymorphism,SNP)进行基因分型,分型结果通过判别函数、ITO法、X染色体STR位点和线粒体SNP分析的方法进行同胞关系判定。结果 39个常染色体等位基因位点的共享基因数为27个,用辨别函数判别归于无关个体;用ITO法计算亲缘关系系数(FSI)为6.95003×10~(-44),判别为无关个体;12个X染色体STR等位基因位点中,有3个X染色体STR位点不匹配,排除姐妹俩来自同一父亲;线粒体SNP的检测显有3个基因座不同,两人来源于不同母系。结论通过联合利用常染色体STR、X染色体STR和线粒体SNP检测技术进行确认两人不是同胞姐妹关系。
Objective To carry out a sibling identification on the orphaned sisters. Methods Genomic DNA of two ladies were extracted by using chelex-100 method. Genotyping was performed by detecting STRs loci on autosome, STR loci on X-chromosome and mitochondrial-SNP loci. Then the data were analyzed by discriminant function analysis , ITO method , X-STR analysis and mitochondrial-SNP analysis to identify the sibling relation of the orphaned sisters. Results There were 27 shared loci among 39 STRs loci on the autosome , which were considered as unrelated individuals based on discriminant function analysis and ITO method. 3 inconsistent loci were detected among 12 X-STR loci , which implied that the two sisters didn’t have a biological father. 3 inconsistent loci were detected among 49 mitochondrial-SNP loci, which suggested that they didn’t have a biological mother. Conclusion The joint use of the technology of autosome STR , X-chromosome STR and mitochondrial-SNP determined that two

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目的探讨氨基比林血痕预试验处理血痕后样本DNA含量的变化及对STR分型检测的影响。方法 10名健康无关个体EDTA抗凝血液制成滤纸血痕,氨基比林血痕预试验检测,按试验后血样干燥保存时间分30min、1h、3h、6h、12h、24h共6个实验组,并采用磁珠法、QIAcube DNA纯化法、Chelex-100法三种方法提取样本DNA,应用荧光定量PCR检测样本DNA含量,PCR-STR荧光技术进行STR分型。结果提取方法相同时,氨基比林血痕预试验后血样随干燥保存时间的延长,样本DNA含量呈逐渐降低的趋势。保存时间相同时,不同DNA提取方法间,样本DNA含量差异也有统计学意义。90.56%样本均可获得16个STR基因座明确分型。结论氨基比林血痕预试验对血痕样本DNA有损伤,24 h内多可获有效STR分型。磁珠法提取样本DNA进行STR分型,效果最好。
Objective To study DNA quantification and STR typing of samples pre-treated with pyrami-don. Methods The blood samples of ten unrelated individuals were anticoagulated in EDTA. The blood stains were made on the filter paper. The experimental groups were divided into six groups in accor-dance with the storage time, 30 min, 1 h, 3 h, 6 h, 12 h and 24 h after pre-treated with pyramidon. DNA was extracted by three methods: magnetic bead-based extraction, QIAcube DNA purification method and Chelex-100 method. The quantification of DNA was made by fluorescent quantitative PCR. STR typing was detected by PCR-STR fluorescent technology. Results In the same DNA extraction method, the sample DNA decreased gradually with times after pre-treatment with pyramidon. In the same storage time, the DNA quantification in different extraction methods had significant differences. Sixteen loci DNA typing were detected in 90.56% of samples. Conclusion Pyramidon pre-treatment could cause DNA degradation, but

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目的 提取西安地区出土3000年前人牙齿中的DNA并进行性别鉴定,为后续的考古研究提供帮助.方法 选取西安地区出土的3000年前人牙齿35颗,应用逆向根管技术提取牙齿内部组织的DNA.设计釉原蛋白基因特异性引物,应用PCR技术进行样本的性别鉴定.结果 成功提取到30份3000年前的人基因组DNA,并对相应样本进行了性别鉴定,其中男性8例,女性22例.结论 逆向根管技术可用于古人类牙齿DNA的提取,基因学方法可补充体质人类学方法的不足,完善古代样本性别信息.
Objective To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi''an and determine the genders for the individuals.Methods Thirty-five ancient human teeth were studied.A ‘Reverse-root-canal'' technique and a Chelex-100 solution were used to extract the DNA.Specific primers for Amelogenin gene were designed for PCR amplification.Results Genomic DNA was successfully extracted from 30 samples,for which 8 were determined to be males and 22 were females.Conclusion The "Reverse-root-canal" technique can be used for extracting DNA from ancient human teeth.Genetics method can supplement physical anthropology for sex-determination of ancient samples.

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