探讨脑室内注射5,7-双羟色胺(5,7-DHT)对内侧前额叶皮层(mPFC)锥体神经元5-羟色胺-1A(5-HT1A)受体敏感性的影响,阐明5-HT1A受体对锥体神经元电活动的作用。方法:36只雄性SD大鼠随机分为假手术组(n=21)和5,7-DHT损毁组(n=15)。损毁组大鼠脑室内注射5,7-DHT,假手术组大鼠脑室内注射同等剂量生理盐水,2组大鼠静脉注射不同剂量(0.5~128.0μg·kg-1)5-HT1A受体激动剂8-OH-DPAT,采用体细胞外电生理学方法观察mPFC中锥体神经元放电频率的变化,并静脉注射5-HT1A受体拮抗剂WAY100635,观察损毁组大鼠对5,7-DHT激动剂和拮抗剂的敏感性,并与假手术组进行比较。结果:在假手术组中,不同剂量(0.5~128.0μg·kg-1)8-OH-DPAT对大鼠锥体神经元的放电频率均产生兴奋-抑制式的影响,这些神经元在低剂量(0.5~32.0μg·kg-1)时被兴奋,放电频率增加(P0.05);而在高剂量(128.0μg·kg-1)时则被抑制,放电频率减少。在损毁组中,不同剂量(0.5~128.0μg·kg-1)8-OH-DPAT剂量依赖性地抑制大鼠锥体神经元的电活动(df=5,F=3.44,P=0.003),即放电频率减少,未见兴奋-抑制效应;WAY10035可以反转8-OH-DPAT的抑制作用。结论:脑室内注射5,7-DHT可使大鼠mPFC锥体神经元5-HT1A受体敏感性降低。
Objective To explore the influence of intraventricular injection of 5, 7-drhydroxytryptamine (5, 7-DHT)in 5-HT1A receptor sensitivity of medial prefrontal cortex pyramidal neurons in the rats,and to clarity the effect of 5-HT1A receptor on the eletronic response of pyramidal neurons.Methods 36 male SD rats were randomly divided into sham operation group (n=21)and 5,7-DHT lesion group (n=15).5,7-DHT was injected intraventricularly in the rats in 5,7-DHT lesion group,and the same dose saline was injected in the rats in sham operation group.The rats in two groups were intravenously injected with different doses(0.5-128.0μg·kg-1 )of 8-CH-DPAT.The firing rate of mPFC pyramidal neurons was recorded with extracellular electrophysioological examination.The rats in two groups were intravenously injected with WAY100635,the sensitivites of the rats to 8-OH-DPAT and WAY100635 in 5, 7-DHT lesion group were observed, and compared with sham operation group.Results The different doses (0.5