登录

双语推荐：FITC

进入期刊文章
目的：测定冷藏（4℃）保存48 h后血小板膜糖蛋白GPⅠbα末端糖基脱落情况。方法分别以一定浓度梯度的FITC-sWGA、FITC-RCA-1、FITC-ECA标记血小板，应用流式细胞仪检测，确定最佳工作浓度。检测冷藏保存48 h后血小板GPⅠbα末端唾液酸基及次末端半乳糖基脱落情况。结果 FITC-sWGA、FITC-RCA-1、FITC-ECA工作浓度分别为2μg/ml、2μg/ml、30μg/ml时标记效果好。冷藏保存48 h后GPⅠbα末端唾液酸基及次末端半乳糖基脱落较22℃常规保存明显增加，分别增至（156±18）%及（123±13）%，差异有统计学意义（P＜0.01）。结论冷藏保存48 h可致血小板膜糖蛋白GPⅠbα末端糖基脱落增加。
Objective To measure the exposed residues of platelet membrane glycoprotein GPⅠbαwith flow cy-tometry (FCM) after refrigerating 48 h. Methods A series of concentration was set, and the optimal working concentration of FITC-sWGA, FITC-RCA-1, FITC-ECA was determined respectively. The platelet membrane GPⅠbαterminal exposed residues were analyzed after 22℃ preservation and 4℃ refrigeration respectively. Results FITC-sWGA, FITC-RCA-1 and FITC-ECA worked well at 2μg/ml, 2μg/ml and 30μg/ml respectively. Refrigeration led to increasing of GPⅠbαterminal sugar radicals shedding, terminal sialic acid group shedding increased to (156±18)%(P< 0.01), and penultimate galactose group shedding increased to (123 ±13)%(P< 0.01). Conclusion Refrigerating platelets for 48 h leads to in-creasing of platelet GPⅠbαsubunit terminal sugar radicals shedding.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的 制备功能化纳米氧化石墨烯(nano-GO-Tf-FITC)微粒,并研究其在近红外线(NIR)照射下对脑胶质瘤U251细胞的靶向荧光显像作用. 方法 将氧化石墨烯(GO)超声振荡制得纳米单层GO(nano-GO)微粒,以聚赖氨酸叠氮基团(poly-L-lysine-G3)连接靶向分子转铁蛋白(Tf)和荧光分子异硫氰酸荧光素(FITC),制备出功能化nano-GO-Tf-FITC微粒,应用透射电镜在一定倍数下观察并拍摄样品形貌,测定样品粒径.将培养的脑胶质瘤U251细胞分成3组,即靶向实验组(加入nano-GO-Tf-FITC微粒孵育)、平行对照组(加入抗CD71-FITC试剂孵育)和非靶向对照组(加入nano-GO-FITC微粒孵育),采用荧光显微镜检测其荧光显像. 结果 在高分辨率透射电镜下nano-GO为20～100 nm单片层纳米颗粒,功能化nano-GO-Tf-FIT微粒为分散在＜100 nm的单片层.在荧光显微镜下,靶向实验组和平行对照组U251细胞均呈黄绿色,非靶向对照组U251细胞则无显像. 结论 成功制备了功能化nano-GO-Tf-FITC微粒,并且其对脑胶质瘤U251细胞具有明显靶向显像作用.
Objective To prepare the functionalized nano-graphene oxide (nano-GO) particles,and study their targeted fluorescence imaging effects on glioma U251 cells under near infrared (NIR)irradiation.Methods Single-layer nano-GO particles were obtained after ultrasonic oscillation,and then connected with transferrin (Tf) and fluorescein isothiocyanate (FITC) through poly-L-lysine-G3 to prepare functionalized nano-GO-Tf-FITC particles.Sample morphology was observed and the particle sizes were measured under certain transmission electron microscope (TEM).Glioma U251 cells were employed and divided into targeted experimental group (adding functionalized nano-GO-Tf-FITC particles),parallel control group (adding anti-CD71-FITC reagent) and non-targeted experimental group (adding nano-GO-FITC particles).Fluorescence imaging of these groups was determined by fluorescence microscopy.Results Single-layer particles with a range of 20-100 nm were observed under high TEM,and the functionalized nano-GO-Tf-

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的探讨特异靶向人表皮生长因子受体2（ HER2）七肽分子LTVSPYW偶联超顺磁性氧化铁（ SPIO）纳米颗粒分子探针的构建方法及其表征，以及对体外乳腺癌细胞行靶向MR成像的可行性。方法（1）通过共沉淀法合成表面包被聚乳酸的 SPIO （ PS ）；将 PS 与异硫氰酸荧光素（FITC）标记的七肽分子LTVSPWY（FITC-LTVSPWY）偶联构建分子靶向探针（FITC-LTVSPWY-PS），用透射电子显微镜分别测量PS和FITC-LTVSPWY-PS粒径大小，动态光散射仪检测其粒径分布及表面电位，3.0 T MRI检测弛豫率。（2）制备高表达HER2的人乳腺癌细胞株MCF-7爬片，与FITC-LTVSPWY-PS进行孵育，荧光显微镜观察细胞与探针的结合情况，并行普鲁士蓝染色验证。（3）将合成的2种探针分别与HER2高表达的MCF-7细胞及HER2阴性的MDA-MB-231细胞孵育，消化离心后置入1.5 ml Eppendorf管。 FITC-LTVSPWY-PS与MCF-7孵育作为实验组，PS与MCF-7孵育作为对照组，不添加探针的MCF-7细胞为空白组，每种处理设立3管，采用3.0 T MR扫描1次，采集T2 WI序列图像，观察各组细胞MR信号并测出T2信号值，检测分子探针的靶向增强作用。各组间T2信号值的比较采用单因素方差分析法。结果分子靶向探针构建成功，电子透射显微镜测得PS纳米粒子的核心粒径大小为（13.9±1.6） nm，动态光散射仪测得PS的表面粒径为（122.0±5.5） nm。 FITC-LTVSPWY-PS探针的表面电位与弛豫率分别为（-30.7±2.2） mV和70.7 m· M-1· s-1，PS的表面电位与弛豫率分别为（28.1±2.8） mV和72.1 m· M-1· s-1。25μg/ml FITC-LTVSPWY-PS与MCF-7细胞孵育1 h后体外细胞MRI显示，实验组呈短T2低信号，对照组与空白细胞组呈等信号。实验组、对照组和空白组T2信号值分别为（61.8±5.7）、（101.6±2.5）和（103.5±1.9）ms，差异有统计学意义（ F＝355.698，P＜0.05）。结论针对HER2与聚乳酸修饰的SPIO耦联后获得的MR靶向探针具有良好的物理学表征与磁学特性，可与HER2阳性细胞特异性结合，满足靶向成像的基本要求。
Objective To develop a superparamagnetic iron oxide nanoparticles ( SPIO ) based on MRI probe specifically targeting human epidermal growth factor receptor 2 (HER2) and explore its value as MRI positive contrast agents in vitro.Methods (1) The superparamagnetic iron oxide ( PS) was obtained by means of classical coprecipitation in polylactic acid solution , then coupled with fluorescein isothiocyanate (FITC) labeled LTVSPYW to develop the targeted probe ( FITC-LTVSPWY-PS).The particle size was measured under transmission electron microscope.Relaxation rate was detected by 3.0 T MR scanner.(2) Climbing films of human breast cancer cell MCF-7 were prepared and incubated with FITC-LTVSPWY-SPIO, then fluorescence distribution was observed under inverted microscope.And distribution of iron particles was confirmed by prussian blue staining.(3) MCF-7 and MDA-MB-231 cells were incubated withFITC-LTVSPWY-SPIO and PS, respectively.MCF-7 incubated with FITC-LTVSPWY-PS were used as experimental gr

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的 研究胆固醇基修饰的普鲁兰(CHSP)纳米粒的体外HepG2细胞的摄取机制及亚细胞分布.方法 采用异硫氰酸荧光素(FTTC)标记CHSP,透析法制备FITC标记的CHSP(FITC-CHSP)自组装纳米粒并进行表征.采用MTT法考察CHSP纳米粒对HepG2细胞的毒性.选用选择性的内吞途径抑制剂氯丙嗪、菲律宾菌素和阿米洛利来研究HepG2细胞摄取CHSP纳米粒的机制.最后,采用细胞免疫荧光法对内质网、高尔基体和溶酶体进行染色,使用激光扫描共聚焦显微镜(CLSM)观察不同的孵育时间CHSP纳米粒的亚细胞分布.结果 成功合成了FITC-CHSP,且FITC-CHSP能在水介质中自组装成纳米粒,该纳米粒呈规则球形,平均粒径为(63.0 ±1.9) nm.MTT结果表明CHSP纳米粒对HepG2细胞无明显的细胞毒性.内吞抑制实验表明网格蛋白介导的内吞途径以及巨胞饮途径共同参与了CHSP纳米粒的入胞过程.纳米粒的亚细胞分布实验表明:在研究的孵育时间(4 h)内,并未发现CHSP纳米粒进入高尔基体和内质网;当纳米粒与HepG2细胞孵育30 min时,没有纳米粒定位于溶酶体中,随着孵育时间的延长,大量纳米粒分布于溶酶体中.结论 CHSP纳米粒有望成为细胞内递送治疗剂的一种通用载体.
Objective To investigate the cellular uptake mechanism and subcellular distribution of cholesterol-modified pullulan (CHSP) nanoparticles by human hepatocellular carcinoma (HepG2) cells.Methods Fluorescein isothiocyanate (FITC) was conjugated to CHSP,and FITC-labeled CHSP (FITC-CHSP) nanoparticles were prepared by dialysis.The cytotoxicity of CHSP nanoparticles against HepG2 cells was evaluated by MTT assay.Meanwhile,several selective endocytosis inhibitors (chlorpromazine,filipin and amiloride) were selected to study the potential endocytosis mechanism.To study the subcellular distribution of CHSP nanoparticles at different incubation times,immunofluorescence staining was used to identify the Golgi apparatus,endoplasmic reticula (ER) and lysosomes,followed by confocal laser scanning microscopy(CLSM) observation.Results Covalent conjugation with FITC yielded stably labeled CHSP,which was successfully formulated into nanoparticles (mean particle size (63.0±1.9) nm) by dialysi

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的探讨胸腺基质淋巴细胞生成素(TSLP)对正常人支气管上皮细胞(16HBE)通透性的影响。方法体外培养16HBE,待细胞状态稳定后,用四唑盐(MTT)比色法检测TSLP(0.1、1、10、100 ng/ml)对16HBE细胞活力的影响。使用无血清1640培养基同步化12 h后,分别加入TSLP刺激液0.1 ng/ml和1ng/ml,分别刺激0、0.5、6、12、24 h,使用跨细胞电阻(TER)及异硫氰酸萤光素-右旋糖酐(FITC-DX)透过率检测不同时间及浓度下的TSLP刺激对16HBE单层细胞通透性的影响。Western blotting法检测TSLP对16HBE粘附连接蛋白E钙粘蛋白(E-cadherin)表达的影响。结果 1 ng/mlTSLP刺激对16HBE细胞活力无影响,10 ng/mlTSLP可显著促进细胞增殖(P0.05);浓度为0.1 ng/ml及1 ng/ml的两个TSLP处理组与对照组相比,TER值均显著升高(P0.001),FITC-DX透过率均明显下降(P0.05),本效应无明显浓度依赖;E-cadherin表达在0.1 ng/mlTSLP处理组较其余两组明显升高;TSLP处理不同时间后,处理24 h组的TER值及E-cadherin表达量较其余组显著升高(P0.05),FITC-DX透过率在处理12 h组及24 h组均明显下降(P0.05),其处理12 h组效果更明显。结论 TSLP可明显改善正常支气管上皮屏障功能。
Objective To investigate the effect of thymic stromal lymphopoietin (TSLP) on the permeablily of monolayer bronchial epithelial cells in vitro. Methods Cultured human bronchial epithelial cell line 16HBE was exposed to 0.1 or 1 ng/ml TSLP for 0, 0.5, 6, 12, or 24 h, and the epithelial monolayer permeability was assessed by measuring transepithelial electrical resistance (TER), permeability to FITC-labeled dextran (FITC-DX) and expression of E-cadherin. Results Compared with the control cells group, 16HBE cell monolayer showed significantly increased TER (P<0.001) and decreased FITC-DX fluorescence in the lower chamber (P<0.05) following exposure to 0.1 and 1 ng/ml TSLP, but these changes were not dose-dependent. Exposure to 0.1 ng/ml TSLP resulted in significantly increased expression of E-cadherin. The 16HBE monolayer exposed to 0.1 ng/ml TSLP for 24 h showed the most obvious increase of TER and E-cadherin expression (P<0.05);FITC-DX fluorescence level was markedly decreased after TSL

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：研究硼酸-硼砂缓冲液制备的尿酸酶-过氧化氢酶脂质体（BUCLP）中尿酸酶的体外特性。方法采用逆向蒸发法制备BUCLP，并考察其部分理化性质特性。结果 BUCLP中尿酸酶最适温度保持在40℃，最适pH值由8.5降为8.0，而Km值也由14.207μmol/L降为13.623μmol/L；尿酸酶-过氧化氢酶（UAC）与空白纳米脂质体作用后，再与FITC结合，其荧光强度高于游离UAC与FITC结合的荧光强度，且BUCLP在280 nm处的荧光强度高于游离UAC。结论尿酸酶和过氧化氢酶联合制备成BUCLP后尿酸酶的活性增加。
Objective To characterize the property of uricase loaded in uricase-catalase liposomes (BUCLPs) prepared using borate buffer. Methods BUCLPs were prepared using reverse-phase evaporation, and the physicochemical properties of uricase in the prepared BUCLPs were examined. Results The optimal temperature of BUCLP and URI was 40 oC, their optimal pH values were 8.0 and 8.5, and their Michaelis-Menten constants were 14.207 μmol/L and 13.623 μmol/L, respectively. Fluorescence intensity of nanoliposome-loaded uricase-catalase that bound to FITC was higher than that of uricase-catalase binding directly with FITC; the fluorescence intensity of BUCLP was higher than that of free uricase-catalase at 280 nm. Conclusion Uricase activity is enhanced after loading in uricase and catalase liposomes.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
基于核酸适体对靶标的特异性识别和辣根过氧化物酶(HRP)的高效催化反应,发展了一种用于检测三磷酸腺苷(ATP)的酶联核酸适体分析新方法.核酸适体和靶标的特异性结合导致与核酸适体杂交的短链DNA解链,解离的DNA通过杂交被固定在另一酶标板的DNA捕获.解离的DNA预先标记了异硫氰酸荧光素(FITC)基团,FITC特异性结合HRP标记的FITC抗体,HRP作为信号传导元素催化四甲基二苯胺(TMB)底物显色,通过颜色变化及450 nm波长处吸光度的变化检测ATP.该方法对ATP具有良好的选择性,检测不受其它物质如GTP,UTP和CTP的干扰,且检测能在较复杂的试样(体积分数10%和50%的血清)中进行.实验结果表明,在ATP浓度为50~400 nmol/L范围内,具有良好的线性关系,检出限为26 nmol/L.
A novel aptamer-based enzyme-linked assay for the detection of adenosine triphosphate(ATP) was established based on the specific recognition of aptamer towards target and the efficient catalytic ability of horseradish peroxidase(HRP). Aptamer binding with targets caused that duplex DNA containing short chain DNA and aptamer dissociated and dissociated DNA was captured by another DNA immobilized on other plate through hybridization. A fluorescein isothiocyanate(FITC) was tagged on dissociated DNA, a HRP as signal transduction element was linked on microplate through specific interactions with anti-FITC-HRP and catalyzed methyenedianiline(TMB) substrate to produce color change. The detection of ATP was achieved by color change and absorbance change at 450 nm. This method has good selectivity for ATP recognition interfering from other proteins such as GTP, UTP and CTP and the detection can be carried in complex sample such as 10% and 50% serum. Experimental results show that there is a go

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：建立大鼠重症急性胰腺炎（SAP）模型，观察FITC标记的大肠杆菌突破肠屏障及其发生细菌移位的时间及途径。方法：健康雄性Wistar大鼠50只，随机分为SAP组（n=30）和假手术组（n=20）；造模前3 h用FITC标记的大肠杆菌给大鼠灌胃，分别在造模后2、3、4、6、8h处死动物，分别取胰腺、肠系膜淋巴结制备组织匀浆；另取门静脉血及腹水，离心取上清，荧光化学发光检测仪检测标本中FITC的荧光强度，确定细菌移位出现的途径及时间。结果：与假手术组相比，SAP组于造模后3 h在腹水，4 h在胰腺组织，6 h在肠系膜淋巴结中检测出较高强度的荧光（P＜0.05），并以腹水中的荧光强度为最高；门静脉血至造模后8 h仍未检测到细菌移位。结论：腹水和淋巴液可能是SAP早期细菌移位的重要途径，细菌可通过上述途径早期发生移位。
Objective To study the time and route of bacterial translocation when FITC-labeled E.coli went through the intestinal barriers of rats with Severe Acute Pancreatitis (SAP). Methods Fifty healthy male wistar rats with weight of (250 ± 30) g were randomly divided into a SAP group (n=30) and a sham-operated group (n=20). FITC-labeled E.coli were given by gavage 3 hours before surgery. The rats were killed at 2 h, 3 h, 4 h, 6 h and 8 h after SAP was induced. Their pancreas and mesenteric lymph nodes were removed. The route and time of bacterial translocation were examined. Results Compared to the sham-operated group, a higher fluorescence intensity (P<0.05) in ascitic fluid at 3 h, in pancreas at 4 h, and in mesenteric lymph nodes at 6 h in SAP group was detected. The fluorescence intensity in ascitic fluid was the highest. But bacterial translo?cation in the portal vein blood 8 hours after SAP was did not detected. Conclusion SAP can lead to impair?ment of intestinal barrier. A

[ 可能符合您检索需要的词汇 ]

进入期刊文章
Southern杂交作为黄金标准，已广泛运用于DNA的检测上。但是，经典的southern杂交和近年来一些DNA检测方法存在放射性污染，操作繁琐，耗时，对实验仪器设备要求高等问题。本文利用异硫氰酸荧光素（FITC）标记的dUTP 合成DNA探针建立了快速检测DNA的液相杂交方法。该方法包括探针制备、液相杂交、电泳分离和信号检测四个步骤，并在此基础上对FITC-双链和FITC-单链探针的杂交效果作了比较。结果显示，使用FITC标记的两种探针都能取得良好的实验结果，但单链探针较双链的检测灵敏度高；双链DNA探针可以检测出0．8μg（3．64×10^-13 mol）的质粒，而单链DNA探针可以检测出0．38μg（1．82×10^-13 mol）的质粒DNA，在检出效率上是前者的2．1倍。整个检测过程操作简便，可在3 h内完成，可较好地解决了其他DNA检测方法存在的费时费力的问题。
Traditional Southern blot,as the golden standard to detect DNA,is widely used in molecular biolo-gy.However,its operation process has some significant disadvantages,such as radioactive contamination, complex procedures,time-consuming.Some new methods developed in recent years need expensive equipments and also have the defects of complex procedures and time-consuming.The paper described a method to detect DNA quickly by liquid hybridization.The probe used is a fragment of DNA sequence labeled by FITC-12-dUTP.The whole process includes four steps:probe preparation,hybridization,electrophoresis and signal de-tection.The comparative experiments using the single strand DNA probe and the double strand DNA probe in-dicated that the sensitivity of single strand DNA probes,which could detect 0. 38μg (1. 82 ×10-13 mol)plas-mid,is 2. 1 times than that of the double strand DNA probes which could detect 0. 8μg(3. 64×10-13 mol) plasmid.The total procedure is simple and can be completed in 3 h.

[ 可能符合您检索需要的词汇 ]

进入期刊文章
目的：体外分离、扩增培养大鼠骨髓源性内皮祖细胞，并进行生物学特性鉴定，为后续实验研究提供材料准备。方法：采用密度梯度离心法和差速贴壁法分离获得单个核细胞，置于特殊培养基EGM -2MV进行扩增培养，对细胞行免疫荧光法检测CD34、CD133、VEGFR2的表达情况，摄取DiI-ac-LDL、结合FITC -UEA-1双染色鉴定及细胞迁移实验。结果：刚分离的单个核细胞小而圆，诱导培养4d左右细胞开始变大，部分呈梭形或多角形，2周左右呈条索状排列；细胞免疫荧光法鉴定CD34、CD133、VEGFR2为阳性，细胞既能吞噬DiI -ac -LDL又能与FITC-UEA-1结合；细胞迁移实验可见每个视野迁移细胞数为（16．6±1．05）个。结论：通过密度梯度离心法和差速贴壁法能成功获取单个核细胞，在特定培养基EGM -2MV中培养后可分化成为内皮祖细胞，为后续实验提供了细胞来源。
experiment .Methods :Mononuclear cells were collected by density gradient centrifugation and induced culture in EGM -2MV ,then use immunofluorescence to Objective :Isolation , culture and identification of endothelial progenitor cells from SD rat bone marrow in order to supply the subsequent identify cell makers such as CD 34、CD133、VEGFR2 ,then observe cell stained DiI -ac-LDL and FITC -UEA-1 ,and tested the ability of migration .Results :Adherent cells were expressed CD34、CD133、VEGFR2 related antigen and labeled both with DiI -ac-LDL and FITC -UEA-1 ,the migranted cells were (16 .6 ± 1 .05) .Coclusions :The mononuclear cells collected from SD rat bone marrow by density gradient centrifugation can be differentiated into endothelial progenitor cells ,and this method applied cell source for subsequent experiment .

[ 可能符合您检索需要的词汇 ]