目的：比较干扰素释放酶联免疫法（TB-IGRA）与蛋白芯片两种方法检测结核分枝杆菌的特异度、敏感度，评价其在结核病诊断中的价值。方法选取2013年10月-2014年3月收集共计78例临床诊断结核病及正常人外周血标本，分别采用TB-IGRA试剂盒和结核分枝杆菌 IgG抗体检测试剂盒（蛋白芯片）进行检测，并对检测结果进行分析比较。结果蛋白芯片法、TB-IGRA法检出结核分枝杆菌的灵敏度分别为34.5％及89.7％，差异有统计学意义（χ2＝26.95，P＜0.05）；二者的特异度分别为90.0％及95.0％，差异无统计学意义（χ2＝1.64，P＞0.05）。TB-IGRA法在肺结核和肺外结核患者阳性率分别为90.5％，89.2％，均高于蛋白芯片法（芯片法在肺结核和肺外结核患者阳性率分别为42.9％，29.7％）。结论 TB-IGRA法与蛋白芯片法比较，无论是诊断肺结核或肺外结核均具有阳性率高、敏感度较高的优点，可广泛用于结核病的早期筛查。

Objective To evaluate the advantages of TB-IGRA and protein chip to detect the Mycobacterium tuberculosis. Methods From October 2013 to March 2014,collected 78 cases of clinical diagnosis of tuberculosis and normal control’s pe-ripheral blood specimens,used TB-IGRA kits and Mycobacteriumtuberculosis IgG kit(protein chip)to detected respectively. The results were analyzed and compared.Results The sensitivity of protein chip and TB-IGRA in the detection of Mycobac-teriumtuberculosis were 34.5% and 89.7% respectively,which was statistically significant (χ2=26.95,P 0.05).The positive rate of TB-IGRA and Protein chip in tuberculosis were 90.5% and 42.9%.The positive rate of TB-IGRA and Protein chipin extrapulmonary tuberculosis were 89.20% and 29.7% respectively.Conclusion Compared TB-IGRA and protein chip,either diagnose tuberculosis or extrapulmonary tuberculosis has highly positive rate and sensitivity, TB-IGRA can be widely used in the early screening of tuberculosis.

目的：了解人群聚集场所人员结核分枝杆菌潜伏感染状况。方法使用结核感染t细胞检测试剂盒(tB-iGra),对人群聚集场所人员组（92例）、活动性肺结核组（78例）及医疗卫生人员组（23例）分别进行tB-iGra试验,了解各组结核分枝杆菌潜伏感染情况。结果193例受检人员中,128例iGra试验阳性,总阳性率66.3%。人群聚集场所人员iGra试验阳性率为65.2%（60/92）；活动性肺结核组iGra试验阳性率79.5%（62/78）；医疗卫生人员iGra试验阳性率26.1%（6/23）。结论人群聚集场所人员结核潜伏感染率高，应加强相关防护措施。

Objective to study the latent tuberculosis infection (ltBi) in congregate population. Methods a total of 193 cases, including 92 people in a congregate place, 78 patients with active pulmonary tuberculosis and 23 health care workers, were collected respectively to detect Mycobacterium tuberculosis infection through gamma interferon release assay (tB-iGra). Results 128 cases’ tB-iGra results were positive and the positive rate was 66.3%. the tB-iGra positive rate in the congregate workers was 65.2%(60/92), the positive rate of patients with active pulmonary tuberculosis and the health care workers was 79.5%(62/78) and 26.1%(6/23), respectively.Conclusion there is high latent tuberculosis infection rate in congregate people, therefore, essential protective measures should be applied.

目的探讨γ干扰素试验在幼儿隐性结核感染诊断中的临床价值。方法选取2012年1月~2013年3月来十堰市太和医院诊治的疑似隐性结核感染的患者33例和活动性结核感染患者35例作为观擦组,另外选取同一时期来十堰市太和医院体检的30名健康儿童作为对照组,分别对以上3组进行痰涂片分析、结核菌素皮肤试验(tuberculin skin test,TST)和γ干扰素释放试验(interferon gamma release assay,IGRA),比较不同方法阳性率。结果在疑似隐性结核感组中,IGRA的检测结果显著高于TST和痰涂片分析(P0.05);在活动性结核感染组中,IGRA的检测结果显著高于TST和痰涂片分析(P0.05);在健康对照组中,TST的检测结果高于IGRA,这与研究对象曾接种过卡介苗有关。IGRA在疑似隐性结核感组和活动性结核感染组检测阳性率分析,P0.05。结论 y干扰素释放试验可作为隐性结核感染诊断的方法之一。

Objective To explore the clinical value of interferon gamma release assays in early childhood diagnosis of latent tuberculosis infection.Methods 33 patients with suspected latent tuberculosis infection and 35 patients with active tuberculosis infection in our hospital from January 2012 to March 2013 were selected.During the same period selected 30 healthy children as a control group.Respectively were given sputum smear analysis,tuberculin skin test (TST)and vinterferon release assay (IGRA).Compare the positive rate of different methods. Results In suspected latent tuberculosis group,IGRA test results were significantly higher than TST and sputum smear method(P<0.05);in active tuberculosis infection group, IGRA test results were significantly higher than TST and sputum smear(P<0.05 );in healthy control group,TST test results were higher than the IGRA,it is because of patients had been vaccinated with BCG.Conclusion IGRA can be used as one way to diagnosis the latent tuberculosis infecti

目的探寻γ-干扰素体外释放试验在结核诊断中的应用价值方法 34例菌阳肺结核患者,238例菌阴肺核患者,33例非结核患者,30例健康体检者,分别进行血清结核杆菌抗体检测、结核菌素试验（TST）以及结核分枝杆菌相关γ-干扰素体外释放酶联免疫试验（TB-IGRA）,对其检测结果进行统计分析。结果TB-IGRA、血清结核杆菌抗体试验以及TST的诊断敏感性分别为87.13%、76.10%和64.71%;诊断特异性分别为90.48%、66.67%和71.43%;阳性预测值分别为97.53%、90.79%和90.72%;阴性预测值分别为61.96%、39.25%和31.91%;诊断效率分别为87.77%、74.33%和65.97%。TB-IGRA的诊断敏感性和诊断特异性均显著高于血清结核杆菌抗体试验以及TST（P〈0.01）。结论 TB-IGRA对结核的诊断具有较高敏感性和特异性,对于菌阴肺结核的辅助诊断和疑似结核感染患者的排除优于其它两种方法。

Objective To evaluate the clinical value of IFN-γrelease assay in the diagnosis of tuberculosis. Methods The study selected 34 patients with smear positive pulmonary tuberculosis, 238 patients with smear nega-tive pulmonary tuberculosis, 33 patients with non-tuberculosis and 30 healthy people. They were given mycobacterium tuberculosis antibody detection, tuberculin skin test ( TST) and TB-IGRA respectively. Results The diagnostic sen-sitivity of TB-IGRA, mycobacterium tuberculosis antibody test and TST was 87. 13%, 76. 10% and 64. 71% respec-tively. The diagnostic specificity was 90. 48%, 66. 67% and 71. 43%, the positive predictive value was 97. 53%, 90. 79% and 90. 72, the predictive valueswere 61. 96%, 39. 25% and 31. 91%, and the diagnosis rate was 87. 77%, 74. 33% and 65. 97%. The diagnostic sensitivity of TB-IGRA were higher than mycobacterium tuberculo-sis antibody test and TST (P<0. 01). Conclusion TB-IGRA for the diagnosis of tuberculosis is highly sensitivity and

目的：探讨体外释放酶联免疫法检测结核杆菌γ-干扰素（TB-IGRA）对结核性胸腔积液的诊断价值。方法收集济宁医学院附属医院呼吸科2014年1～9月诊治的55例结核性和49例恶性胸腔积液患者的病例资料，对患者进行 TB-IGRA、细菌涂片镜检、结核菌素皮试（PPD）和结核杆菌抗体等检测，并对以上诊断方法进行比较分析。同时联合检测血清癌胚抗原（CEA）及胸腔积液腺苷脱氨酶（ADA），以评估 TB-IGRA 联合 CEA 和 ADA 对鉴别诊断结核性胸腔积液的意义。采用 SPSS 17．0统计软件进行数据分析，阳性例数以率表示，并进行卡方检验。CEA 和 ADA 的测定值用均数±标准差表示，显著性检验用 t 检验。结果55例结核组患者 TB-IGRA 阳性53例，达96．36％；49例恶性组患者 TB-IGRA 阳性1例，仅2．04％，两组差异有统计学意义（χ2＝88．62，P＜0．05）。结核组血清CEA 水平[（2．34±1．80）μg/L]显著低于恶性组[（2358．68±1069．40）μg/L，t ＝-15．42，P ＜0．05]；胸腔积液 ADA 水平[（57．51±26．90）U /L]显著高于恶性组[（14．43±3．57）U /L，t ＝11．76， P ＜0．05]。结核组中 TB-IGRA、涂片镜检、PPD 试验以及结核杆菌抗体的阳性率分别为96．36％、22．64％、46．67％和

Objective To evaluate the clinical significance of mycobacterium tuberculosis(MTB) related interferon-gamma (IFN-γ)in vitro by enzyme-linked immunosorbent assays for the diagnosis of tuberculous pleural effusion.Methods The clinical data with fifty-five cases with tuberculous and forty-nine cases with malignant pleural effusion were diagnosed and treated in Affiliated Hospital of Jining Medical University from January to September in 2014.These patients were detected by diagnostic kit for mycobacterium tuberculosis correlative IFN-γrealse assay(TB-IGRA),bacterial smear,purified protein derivative test and mycobacterium tuberculosis antibodies.Then the above diagnostic methods were compared and analyzed. Serum carcinoembryonic antigen (CEA)and pleural fluid adenosine deaminase (ADA) together were detected,and the clinical value of TB-IGRA,CEA and ADA for differential diagnosis of tuberculous pleural effusion assessed.Results There were fifty-three TBIGRA positive cases of fi