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双语推荐：II

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建立碱性橙II的酶联免疫分析方法。采用戊二醛偶联法将半抗原碱性橙II与载体牛血清白蛋白（BSA）及卵清蛋白（OVA）制备免疫抗原碱性橙II-BSA和包被抗原碱性橙II-OVA。经紫外可见吸收光谱鉴定和大白兔免疫实验证实人工抗原成功合成，并制备抗碱性橙II的多克隆抗体，该抗体可检测快速定量食品中的碱性橙II，最低检测限为10 pg。该检测方法具有高灵敏度和特异性，可应用于食品中碱性橙II检测试剂盒的研制。
@@@@In order to determined the Chrysoidin II by enzyme-linked immunosorbent assay (ELISA) method, a complete antigen Chrysoidin II -BSA and a coating antigen Chrysoidin II -OVA were prepared by coupling Chrysoidin II with the carrier proteins of bovineserum albumin (BSA)and ovalbumin (OVA) using glutaraldehyde method, respectively. The synthesis of Chrysoidin II artificial antigens was confirmed by UV absorption spectra and animal experiments. A novel anti-Chrysoidin II polyclonal antibody was prepared , which was successfully applied to the determination of Chrysoidin II in food with a limit of detection of 10 pg. High sensitivity and specificity will contribute to the development of commercial ELISA kits for the detection of Chrysoidin II in foods.

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选取Altera公司Cyclone II系列EP2C8Q208芯片作为新型高速数字喷墨印刷机控制系统的开发平台,以μC/OS-II作为印刷机控制的实时系统,通过Nios II 11.0 Eclipse,运用Nios II软核处理器技术来生成μC/OS II实时操作系统模块,通过系统编程来实现新型数字喷墨印刷机控制系统。通过软硬件测试,验证了该系统的稳定性和可操作性。
This paper designs a new high-speed inkjet printers on the platform of Cyclone II series EP2C8Q208 of Altera corporation, utilizing μC/OS-II operating system, and Nios II 11.0 Eclipse tools and various techniques like Nios II soft-core processor in order to generate μC/OS-II real-time operating system module. Besides, combining with system programming that realize a new high-speed control system of inkjet printers.Testing demonstrates that the proposed system is able to exhibit good performance in terms of stability and operability.

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使用乙二胺改性的木屑黄原酸盐对水溶液中的Cu(II)、Ni(II)离子进行吸附。在单离子体系中，考虑影响因素(温度、投加量)对Cu(II)、Ni(II)单离子吸附等温线的影响；并计算Cu(II)、Ni(II)离子吸附的热力学参数：吸附吉布斯自由能(?GΘ)、吸附过程的焓变(?HΘ)以及熵变(?SΘ)，表明此吸附是一个放热自发的过程。在 Cu(II)和Ni(II)双离子体系中，采用修正后的拓展Langmuir模型对体系的吸附情况可以进行很好的预测。在单离子体系和双离子体系中，吸附过程的数据均可通过准二级动力学模型进行描述；计算得到其对Cu(II)和Ni(II)单离子的吸附活化能分别为59.12和55.92 kJ/mol。结果表明，金属离子在改性木屑表面的吸附效果会受到另一离子存在的影响。
Sawdust xanthate modified with ethanediamine was used for the removal of Cu(II) and Ni(II) from aqueous solution. The influence of various operating parameters such temperature and adsorbent dosage on the adsorption isotherms of modified sawdust was investigated. Thermodynamic parameters, namely Gibbs free energy (?GΘ), enthalpy (?HΘ) and entropy (?SΘ) of Cu(II) and Ni(II) adsorption process were calculated, showing that the adsorption is a spontaneous and exothermic process. The modified extended Langmuir equation approaches provide excellent prediction of the binary adsorption. In single and binary systems, the overall adsorption data were best described by the pseudo-second order kinetic model, then the calculated values of activation energy of Cu(II) and Ni(II) adsorption process were 59.12 and 55.92 kJ/mol respectively. The results show that the affinity of each metal ion onto the modified sawdust surface is influenced by the presence of the other one.

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目的：：探讨血管紧张素(angiotensin，ang) II、醛固酮及其拮抗剂干预对大鼠心脏成纤维细胞(cardiac fibroblasts，CFs)活性和胶原蛋白表达的影响。方法：采用胶原酶II消化法、差速贴壁法、差速脱壁法获取并纯化SD乳鼠CFs，将3，4代CFs分为：ang II组、醛固酮组、ang II+醛固酮组、ang II+氯沙坦组、醛固酮+螺内酯组、对照组。采用CCK-8活细胞计数试剂盒检测细胞活性；RT-PCR检测细胞中I型胶原前胶原A1(collagen，type I，alpha 1， COL1A1)、III型胶原前胶原A1(collagen，type III，alpha 1，COL3A1)以及MMP1，基质金属蛋白酶的组织抑制剂(tissue inhibitor of metalloproteinases，TIMP1)mRNA的变化；Western印迹检测COL1A1，COL3A1，MMP1，TIMP1蛋白表达的变化。结果：与对照组比较，ang II、醛固酮可明显促进CFs增殖(增殖率分别为38.5%，28.5%；P<0.05)，ang II+醛固酮组的增殖率较ang II、醛固酮单独干预组更高(增殖率54.4%，P<0.05)，氯沙坦、螺内酯可显著抑制ang II、醛固酮诱导的细胞增殖效应(P<0.05)；与对照组比较，ang II，醛固酮可显著促进COL1A1，COL3A1，MMP1 mRNA和蛋白表达(P<0.05)，同时抑制TIMP1 mRNA和蛋白表达(P<0.05)，氯沙坦、螺内酯可显著抑制ang II、醛固酮诱导的COL1A1， COL3A1，MMP1 mRNA和蛋白表达的增加(P<0.05)，同时升高TIMP1 mRNA和蛋白表达(P<0.05)。结论：Ang II、醛固酮可促进体外培养CFs增殖和增加I，III型胶原表达；Ang II、醛固酮同时干预时具有协同和叠加效应，而氯沙坦、螺内酯可抑制这一效应；其作用机制可能通过影响MMPs/TIMPs的平衡，使心胶原代谢紊乱，增加CFs活性，促进心纤维化。
Objective:To investigate the effect of angiotensin II (ang II), aldosterone (ald) and their receptor antagonists losartan (los) and spironolactone (spi) on the proliferation and collagen production of cardiac ifbroblasts (CFs) in rats. Methods:CFs were isolated from neonatal SD rats by collagenase II method and puriifed with differential attachment and detachment method. The 3 or 4 passages of the CFs were divided into the following groups:angiotensin II, angiotensin II+aldosterone, aldosterone, angiotensin II+losartan, and aldosterone+spironolactone. The cell viability of the CFs was assessed by Cell Counting Kit-8 (CCK-8) after the drug administration. The mRNA and protein expression levels of COL1A1, COL3A1, MMP1 and TIMP1 were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively. Results:Ang II and Ald facilitated the proliferation rate of the CFs independently compared with that in the control group (38.5%vs 28.5%;P Conclusion:Ang II a

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本文首先介绍了LPC1343的硬件架构和UCOS- II实时操作系统的特点，最后介绍了UCOS- II在LPC1343上移植的方法和注意事项。
The document introduced the hardware structure of LPC1343 and character of real- time Operating system、UCOS- II,then discussed the measures and matters needing attention to transplanting ucos- II.

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深入研究成本低廉的膨润土对水溶液中 Pb(II)的吸附特性,采用 Batch 试验方法,分析了土水比、pH、离子强度、反应时间、温度及 Pb(II)的初始浓度对 Pb(II)在膨润土上吸附性能的影响,并对吸附动力学和吸附平衡试验进行了探讨.Pb(II)在膨润土上去除率与 pH、离子强度有很强的依赖性.当 pH<7时,膨润土对 Pb(II)的吸附主要是离子交换,而当7II)的吸附非常迅速,15 min 去除率就达到50%,120 min内可达到平衡.动力学数据符合伪二级动力学模型,吸附速率常数均随 Pb(II)的浓度增加而减小.Langmuir 模型可成功的拟合等温吸附数据,膨润土对 Pb(II)的吸附量可达27.45 mg/g,升温有利于膨润土对 Pb(II)的吸附.试验结果表明:膨润土可作为防污屏障和降低水环境中重金属污染物的吸附剂.
The adsorption of lead on bentonite is studied by means of the Batch experiment due to its low cost and easy availability. The effects of soil-water content, solution pH, ionic strength, reaction time, temperature and initial Pb(II) concentration on the removal of Pb(II) by use of bentonite, kinetic process and adsorption equilibrium are investigated. The removal of Pb(II) from aqueous solutions by use of bentonite is strongly dependent on pH and ionic strength. When pH<7, the removal of Pb(II) is dominated by ion-exchange on soil surfaces, whereas inner-sphere surface complexation is the main adsorption mechanism when 7

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目的：探讨钩骨体部骨折的临床分型和治疗策略。方法报告41例钩骨体部骨折病例的临床分型与治疗效果。根据骨折线的方位和对钩骨-掌骨关节稳定性的影响进行分型：横行骨折（I型）和纵行骨折（ II型），后者进一步分为三个亚型，II A型为钩骨冠状面劈裂骨折；II B型骨折线为斜形，涉及钩骨关节面背侧较大部分；II C型为钩骨背侧撕脱骨折。本组I型骨折2例，II A型骨折9例， II B型骨折21例，II C型9例。根据骨折类型选择治疗方案，保守治疗6例，手术治疗35例。结果本组患者随访4～48个月，平均（21.2±12.2）个月。至最后一次随访，所有患者对治疗的主观评价为满意或非常满意。骨折愈合率100%，术后握力双侧对比差异无统计学意义，患侧平均握力（40.41±6.18） kg，健侧平均握力（40.26±5.13） kg，P＝0.613。没有出现感染、肌腱粘连和神经损伤等并发症。结论钩骨体部骨折预后较好，II A型和II B型骨折由于稳定性差，一般需要手术治疗，II C型骨折则需评估合并损伤情况和腕掌关节复位后的稳定性来决定是否手术。
Objective To investigate the clinical classiifcation and therapeutical strategy of fractures of the body of the hamate. Methods The clinical classiifcation and therapeutic effects of 41 cases of fractures of the body of the hamate were reported. The fractures of the body of the hamate were classiifed into 2 major groups and 3 subgroups based on the orientation of the fracture line and the effects on the stability of the hamatometacarpal joint. Type I referred to the traverse fracture, and type II referred to the longitudinal fracture. Type II A represented coronal splitting fractures of the hamate, type II B represented oblique fractures with a major part of the dorsal hamate joint involved, and type II C represented avulsion fractures of the dorsal hamate. There were 2 cases of type I, 9 cases of type II A, 21 cases of type II B and 9 cases of type II C in this group. The therapeutical strategy was chosen based on the type of fractures, and the conservative treatment was performed on

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利用离子印迹技术制备了镉（II）离子印迹聚合物，并对其吸附性能进行了详细研究。以镉（II）离子为模板，以3-氨基丙基-三乙氧基硅烷为功能单体，在纳米 TiO2/SiO2表面聚合形成镉（II）印迹聚合物。实验结果表明，与非印迹的聚合物相比较，该印迹聚合物对镉（II）离子具有较好的识别性和选择性。镉（II）印迹聚合物与非印迹聚合物的吸附量分别是42．56 mg ? g -1和13．95 mg ? g -1。该法的检出限为0．11 ng ? mL -1，相对标准偏差为1.79％。将该印迹聚合物用于环境和生物样品中的镉（II）的分离富集和测定，结果令人满意。
A Cd(II) ion imprinted nanometer TiO2 /SiO2 composite material was prepared by surface imprinting technique using Cd(II) ion as the template and 3-aminopropyltriethoxysilane as the functional monomer .With the traditional solid sorbents and non-imprinted polymer particles ,the ion-imprinted poly-mers (IIPs) had higher adsorption capacity and selectivity for Cd (II) .The maximum static adsorption ca-pacity of the imprinted and non-imprinted sorbent for Cd(II) was 42 .56 mg ? g- 1 and 13 .95 mg ? g- 1 ,re-spectively .The detection limit of the method was 0 .11 ng ? mL - 1 and relative standard deviation of the e-leven replicate determinations was 1 .79 % .The method was applied for the determination of Cd(II) in en-vironmental samples with satisfactory results .

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重金属污染的地下水治理不断面临着挑战,尤其是在一些发展中国家。纳米铁颗粒代表新一代环境治理技术,面对最具挑战的环境治理问题能够提供有效的解决办法。在实验室制得纳米铁颗粒,粒径为20~40 nm,比表面积(BET)为49.16m2·g-1。通过考察纳米铁对多种重金属共存水体的去除情况,包括As(III)、As(V)、Cd(II)、Pb(II)、Cr(VI)、Cu(II)和Mn(II),实验结果表明,重金属的去除效果与重金属类型,纳米铁投加量和反应时间有关。通常当纳米铁投加量为1.25 g·L-1时,反应时间在30 min内,纳米铁对水体中质量浓度范围为0.1~1.0 mg·L-1的重金属离子As(III)、As(V)、Cd(II)、Pb(II)、Cr(VI)、Cu(II)和Mn(II)去除率达90%以上,还可获得以下结论:1)纳米铁能同时对As(III)和As(V)去除,而不需要将As(III)预先氧化成As(V);2)纳米铁对重金属去除速率快慢为Cu(Ⅱ)Pb(Ⅱ)Cr(Ⅵ)Cd(Ⅱ);3)纳米铁对重金属去除由刚开始快速消失,到后期缓慢去除的2个步骤组成;4)纳米铁对实际水样中重金属都有很好的去除效果,尤其是对高浓度Mn去除效果更明显,可通过延长处理时间或增加纳米铁的投加量方式,去除率能达99%以上。纳米铁对重金属的去除机理取决于重金属的标准电势,纳米铁对As和Cd(Ⅱ)的去除主要是通过吸
Remediation of groundwater contaminated by heavy metal is a recurring challenge, especially in developing countries. Nanoscale iron particles represent a new generation of environmental remediation technologies that could provide cost-effective solutions to some of the most challenging environmental cleanup problems. This study investigated the use of nZVI particles with the particle size of 20-40 nm and specific surface area (BET) of 49.16 m2·g-1 in removing mixed heavy metal contaminants including As(III), As(V), Cd(II), Pb(II), Cr(VI), Cu(II) and Mn(II) from groundwater. Results showed that the removal efficiencies of heavy metals varied with the metal species, nZVI loading, and reaction time. In most cases, use of 1.25 g·L-1 nZVI resulted in removal efficiencies of more than 90% for Cr(VI), Cu(II), Cd(II), Pb(II), Mn(II), As(III), and As(V) with different concentration levels (0.1-1.0 mg·L-1) in 30 min. The following points may be concluded: 1) Both As(III) and As(V) f

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目的：检测寻常型银屑病皮损中血管紧张素II（ AngII）及血管紧张素转化酶（ ACE）的水平变化。方法：应用荧光定量聚合酶链反应（ FQ-PCR）和免疫组化技术检测30例寻常型银屑病患者（进行期17例，静止期13例）皮损Ang II和ACE mRNA及蛋白质的表达。结果：银屑病患者皮损中Ang II和ACE mRNA及蛋白质的表达明显高于正常人对照组（P〈0.001），且进行期高于静止期（P〈0.001）。结论： Ang II和ACE 在银屑病皮损中表达上调，推测可能与银屑病皮损中新血管的形成有关。
Objective: To detect the level of angiotensin II (Ang II) and angiotensin converting enzyme ( ACE) in patients with psoriasis vulgaris. Methods:The level of Ang II and ACE was detected by fluorescent quantitative polymerase chain reaction ( FQ-PCR) and immunohistochemistry technique in 30 patients with psoriasis vulgaris ( progressing stage in 17 cases and stable stage in 13) and 15 normal controls. Results:The level of Ang II and ACE protein was significantly higher in psoriatic lesions than in controls ( P<0.001) and in progressing stage higher than in stable stage ( P<0.001) . Conclusion:Over expression of Ang II and ACE in psoriatic lesions may be implicated in the neovascularization of psoriasis.

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