目的探索Erbin表达缺失导致Her2阳性的人乳腺癌细胞MDA-453对曲妥珠单抗敏感性的影响。方法设计、构建含人Erbin基因特异性的短发夹RNA(shRNA)及对照shRNA的干扰载体,转染MDA-453人乳腺癌细胞。经G418加压筛选,获得稳定敲低Erbin的MDA-453细胞(MDA-453-Erbin sh)和稳定表达对照shRNA的MDA-453细胞(MDA-453-NC),以后者作为对照细胞。应用Western印迹法检测MDA-453-NC和MDA-453-Erbin sh细胞中Erbin表达水平。分别通过细胞增殖活性实验及细胞集落形成实验,观察敲低Erbin的MDA-453细胞对曲妥珠单抗敏感性的变化。通过免疫组化染色法,分析Erbin在人乳腺癌及正常乳腺组织中的表达。结果建立了稳定敲低Erbin的MDA-453细胞株,发现敲低Erbin表达可诱导MDA-453细胞对曲妥珠单抗产生抗性。与正常乳腺上皮组织相比,人乳腺癌组织标本中Erbin表达水平降低。结论 Erbin表达缺失可能影响乳腺癌对曲妥珠单抗治疗的敏感性。

Objective To explore the effect of Erbin deficiency in MDA-453 cells on trastuzumab(Herceptin) resist-ance .Methods The specific short hairpin RNA ( shRNA) targeting Erbin was designed and cloned into plasmid pSuppres-sor, which was subsequently transfected into MDA-453 human breast cancer cells .After being selected by G418, MDA-453 cells stably expressing Erbin shRNA were obtained and nominated as MDA-453-Erbin sh.The MDA-453 cells express-ing control shRNA ( MDA-453-NC) were used as control cells .The expression of Erbin at the protein level in MDA-453-NC and MDA-453-Erbin sh cells was analyzed by Western blotting .Cell proliferation and colony formation assays were em-ployed to investigate the effect of Erbin knockdown on the sensitivity of MDA -453 cells to trastuzumab in vitro.The levels of Erbin expression in human breast cancer tissue and normal breast tissue samples were evaluated by immunohistochemistry . Results MDA-453 cells, in which Erbin expression was stably knocked-dow

目的探讨mTOR在乳腺癌中的表达及其抑制剂CCI-779对乳腺癌细胞MDA-MB-231增殖及凋亡的影响。方法采用免疫组化SP法检测mTOR蛋白在乳腺癌组织和乳腺癌细胞MDA-MB-231中的表达;MTT法检测mTOR抑制剂CCI-779对MDA-MB-231细胞增殖的影响;应用AnnexinV-FITC/PI法检测CCI-779对MDA-MB-231细胞凋亡的影响。结果 mTOR蛋白在71例乳腺癌组织中的阳性率为54.9%,明显高于32例癌旁组织（阳性率为21.9%）;MDA-MB-231细胞中亦存在mTOR蛋白的表达;mTOR抑制剂CCI-779对MDA-MB-231细胞的增殖具有显著抑制作用,呈浓度和时间依赖性;但CCI-779并不能诱导MDA-MB-231细胞发生凋亡。结论 mTOR与乳腺癌关系密切,其抑制剂CCI-779具有较强的抗MDA-MB-231细胞活性,有望成为乳腺癌治疗的前景药物。

Purpose To investigate the expression of mTOR in breast cancer, and to observe the effect of CCI-779 on proliferation and apoptosis of MDA-MB-231 cell. Methods Immunohistochemical staining was used to detect the expression of mTOR protein in breast cancer tissue and MDA-MB-231 cell. MTT method was used to show effect of CCI-779 on proliferation of MDA-MB-231 cell. Annex-inV-FITC/PI method was used to show effect of CCI-779 on apoptosis of MDA-MB-231 cell. Results 54.9% in 71 cases of breast cancer tissue could express mTOR protein, the expression was significantly higher than in 32 cases of normal tissue (21.9%), mTOR protein was also detected in MDA-MB-231 cell, CCI-779 could inhibit the proliferation of MDA-MB-231 cell, and show a dose-and time-dependent, but CCI-779 could not induce apoptosis of MDA-MB-231 with AnnexinV-FITC/PI assay. Conclusion mTOR is closely related to the formation of breast cancer, CCI-779 has strong activity against MDA-MB-231 cell, it has prospect f

探讨小檗碱对人乳腺癌细胞MDA-MB-231体外生长的影响及其与PPARγ受体的关系。方法:采用噻唑蓝(MTT)法检测小檗碱对MDA-MB-231细胞的生长抑制效应;TUNEL法检测细胞凋亡;联合PPARγ拮抗剂GW 9662,分析小檗碱对MDA-MB-231细胞增殖的影响与PPARγ受体的关系。结果:小檗碱呈量-效和时-效关系抑制MDA-MB-231细胞生长,24 h的半数抑制浓度(IC50)为0.21μmol/L;小檗碱诱导MDA-MB-231细胞凋亡作用随药物浓度的增加而增强;PPARγ受体拮抗剂不能逆转小檗碱对细胞增殖的抑制作用。结论:小檗碱可明显抑制MDA-MB-231细胞生长并诱导其凋亡,但其作用不通过PPAR受体介导。

AIM: To investigate the anti-proliferation effect and its relationship to PPARγ/of berberine on human breast cancer cell line MDA-MB-231. METHODS: Cytostatic effect of berberine on MDA-MB-231 cells was measured by MTT. Apoptotic cells were determined by TUNEL. MDA-MB-231 cells were treated with berbcrine alone or in combination with PPARγ antagonist GW9662 to investigate the effect of berberine on cell proliferation and its relationship to PPARγ. RESULTS: MTT analysis detected that berberine inhibited growth of MDA-MB-231 cells in a dose and time-dependent manner with 0.21 pμmol/L IC50 value at 24 h post-treatment. Apparent apoptosis of MDA-MB-231 cells induced by berberine were also observed. PPARγ selective antagonist GW9662 could not reverse the inhibitory effect of berberine on proliferation of MDA-MB-231 cells. CONCLUSION: Berberine could induce MDA-MB-231 cells''apoptosis and inhibit the cells proliferation with independent of PPARγ activation.

目的探讨荜茇明碱(PL)对人乳腺癌MDA-MB-231细胞增殖、凋亡的影响及作用机制。方法体外培养人乳腺癌MDA-MB-231细胞;采用活细胞计数试剂盒(CCK-8)法检测不同浓度的PL对MDA-MB-231细胞增殖的影响;流式细胞术检测PL对细胞凋亡的影响;蛋白免疫印迹(Western blot)法检测PL对MDA-MB-231细胞Bcl-2和Bax蛋白表达水平的影响。结果PL抑制MDA-MB-231细胞增殖,并呈现出浓度、时间依赖性。PL诱导MDA-MB-231细胞凋亡,而乙酰-L-半胱氨酸则抑制了PL诱导的细胞凋亡。Western blot结果提示随着PL浓度的增加,Bax的表达逐渐增加,而Bcl-2的表达逐渐减少。结论 PL对人乳腺癌MDA-MB-231细胞有明显的生长抑制和诱导凋亡作用,其机制可能与Bcl-2蛋白表达的减少和Bax蛋白表达增加有关。

Objective The aim of the study was to investigate the effect of piplartine on the proliferation and apoptosis in human breast cancer MDA‐MB‐231 cells line and the mechanism involved .Methods Human breast cancer MDA‐MB‐231 cells line was cultured in vitro .The inhibitory effect of piplartine on the proliferation of MDA‐MB‐231 cells was measured by CCK‐8 assay after treatment with piplartine at different concentrations .The apoptosis rates were measured by flow cytometry .Western blot was used to explore the effect of piplartine on MDA‐MB‐231 cells Bcl‐2 and Bax protein expression .Results The CCK‐8 assay showed that piplartine had an inhibiting effect on the proliferation of MDA‐MB‐231 cells in a concentration and time dependent manner .Piplar‐tine induced apoptosis of MDA‐MB‐231 cells obviously .The antioxidant N‐acetyl‐L‐cystein inhibited the apoptosis of cells .By Western blot analysis ,we found the expression of Bax was up‐regulated whereas that of Bcl

目的 探讨断指再植患者术后血清丙二醛(malondialdehyde,MDA)含量及超氧化物歧化酶(superoxide dismutase,SOD)活性变化特点.方法 对40例患者分别于手术后1、24、48、72 h,第7、14 d检测血清MDA含量和SOD活性,另外取30例健康成年人检测血清MDA含量和SOD活性作为对照组进行对比分析.结果 断指再植患者术后1h至7d血清MDA含量逐渐升高,血清SOD活性明显降低,与对照组比较差异有统计学意义(P＜0.05),术后14 d血清中MDA及SOD基本达到对照组水平(P＞0.05).结论 术后检测MDA含量及SOD活性的变化可以指导临床用药,有效地防治血管危象的发生.

Objective To investigate the characteristic changes of serum malondialdehyde (MDA) and supemxide dismutase (SOD) levels in patients who had finger replantation.Methods Blood was drawn from 40 patients at 1,24,48,72 hours and 7,14 days after finger replantation to test the SOD activity and MDA content in the serum.Serum samples were,also collected from 30 healthy adults and analyzed to serve as control.Results Serum SOD activity was significantly reduced,while the serum MDA content increased gradually from 1 hour to 7 days after the operation.There were significant differences when compared to levels in the healthy control (P ＜ 0.05).Serum SOD activity and MDA content basically reached the normal level 14 days later(P ＞ 0.05).Conclusion Detection of serum SOD activity and MDA content after finger replantation can guide medication and prevent probability vascular crisis.