目的了解大连地区2012年感染性腹泻病原菌的种类和流行特征，为感染性腹泻的预防控制和临床治疗提供依据.方法采用常规粪便培养方法，对分离菌做血清学分型，用MicroScan WalkAway-40全自动细菌生化鉴定仪对分离菌做生化鉴定.结果监测29例腹泻患者样本，检出感染性腹泻病原菌10株，阳性率34.48%，其中副溶血弧菌所占比例最大.结论副溶血弧菌是2012年大连地区感染性腹泻的首要病原菌，应依据此监测结果做好2013年大连地区腹泻病的防治工作.

Objective To understand the epidemic character and types of infectious diarrhea pathogens in 2011 in Dalian,and provide references for the prevention and control as well as clinical treatment of infectious diarrhea.Method The pathogen strains were isolated by routine stool culture and made serological typing.The biochemical identification of the isolates were carried out by Automated Microbiology Analyzer MicroScan WalkAway-40. Result 10 intestinal pathogenic bacteria were detected out from 29 samples,and the positive rate was 34.48%,in which Parahaemolyticus accounted for the largest proportion. Conclusion Parahaemolyticus is the primary pathogen caused infectious diarrhea in 2011 in Dalian. Based on the surveillance results,prevention and control work of diarrheal disease in Dalian should be carried out in 2013.

目的对襄阳市中心医院分离1株疑似霍乱弧菌进行鉴定及药物敏感性试验,并检测其主要毒力基因。方法利用MicroScan WalkAway 40鉴定仪进行生化鉴定及药物敏感性试验,玻片凝集法确定其血清型别,应用PCR及测序技术分析其16SrRNA基因;PCR检测其6个毒力基因。结果经鉴定,该株疑似霍乱菌株为非O1群非O139群霍乱弧菌,经16SrRNA分析与美国国家生物技术信息中心数据库中霍乱弧菌相似性达100%。药敏试验结果显示该菌对氨苄西林、氯霉素、甲氧苄啶-磺胺甲(口恶)唑、四环素均敏感,毒力基因检测rtxC和toxR阳性,tcpAET、ctxA、hlyA、tcpACL阴性。结论该株疑似霍乱弧菌为非O1群非O139群霍乱弧菌,其致病与rtxC、toxR毒力基因有关。

Objective To identify an suspected Vibrio cholerae isolated from Xiangyang Central Hospital and characterize the strain in terms of antibiotic resistance and relevant virulence genes.Methods Pathogen identification and susceptibility testing were completed with MicroScan WalkAway 40 Automated Microbiology System.Slide agglutination was used for serotyping. PCR and sequencing technology were employed for 16s RNA gene analysis.PCR technique was used to detect six major viru-lence genes.Results This suspectedVibrio cholerae isolate was confirmed as non-O1 and non-O139 Vibrio cholerae .Suscep-tibility testing results indicated that the strain was sensitive to ampicillin,chloramphenicol,trimethoprim-sulfamethoxazole, and tetracycline.16s RNA gene sequence analysis showed 100% homologous with the registered sequence in National Center for Biotechnology Information database.Virulence genes rtxC and toxR were identified.The other virulence genes such as tcpAET,ctxA,hlyA,and tcpACL were negativ

目的：监测我院2010年-2012年从临床标本中分离的鲍曼不动杆菌及泛耐药鲍曼不动杆菌的检出率及对抗菌药物的耐药情况，为医院感染监测和临床合理用药提供依据。方法收集近3年来我院患者的各类感染标本，经微生物室培养后，用美国DADE-MicroScan Neg Combo Type 31细菌菌种鉴定和最低抑菌浓度（MIC）测定，补充药敏试验用琼脂纸片扩散（K-B）法进行检测。所有数据通过WHONET5.6软件进行统计学分析，以排除重复菌株。结果2010年-2012年鲍曼不动杆菌的检出率分别为9.18%，6.92%，7.19%，其中泛耐药（XDR）鲍曼不动杆菌在所分离出的鲍曼不动杆菌中的检出率分别为12.41%，26.47%，13.01%。结论自2012年我院加强医院感染控制措施，控制药品比例以及加强合理使用抗生素管理以来，虽然鲍曼不动杆菌耐药率总体呈上升趋势，但泛耐药鲍曼不动杆菌的检出率明显降低。

Objective In order to monitor the hospital infection and provide the basis for rational drug use,we monitored the detection rate and drug resistance of Acinetobacter Baumannii and pan-resistant Acinetobacter Baumannii isolated from clinical specimens in our hospital from 2010 to 2012. Methods Various infections specimens of patients in our hospital in the past three years were collected and isolated. Then, the Bacterial strainidentification and the minimum inhibitory concentration (MIC) were carried out by America DADE-MicroScan Neg Combo Type 31. At the same time, the MIC was repeatedlydetected using Agar disk diffusion (K-B) method as supplement. All data were analyzed statistically by WHONET5.6 software for excluding repetitive strain. Result In 2010 ~2012 Bauman Acinetobacter infection rates were 9.18%,6.92%,and 7.19%. The detection rates of pan drug resistant (XDR) Acinetobacter baumannii were 12.41%,26.47%,and 13.01%.Conclusion Since 2012,although we have strengthened the hospita

目的：统计分析重症监护病房（IC U ）不同患者送检标本及环境中分离得到的鲍氏不动杆菌耐药性及同源性，为临床治疗及医院感染管理科感染控制提供依据，切断鲍氏不动杆菌医院感染传播途径。方法应用西门子MicroScan微生物鉴定及药敏系统对来自ICU患者及环境中的鲍氏不动杆菌进行细菌培养鉴定及药敏分析，运用肠杆菌科基因组间重复序列聚合酶链反应（ERIC‐PCR）及琼脂糖凝胶电泳进行同源性分析。结果13株鲍氏不动杆菌中有10株为多药耐药株，其中7株对常用抗菌药物均耐药，有3株分离自IC U患者送检标本、4株分离自IC U环境；同源性分析显示，13株鲍氏不动杆菌共分为4型（A～D ），其中有10株为A型。结论 IC U鲍氏不动杆菌聚集暴发由A1亚型引起，含有鲍氏不动杆菌的气溶胶可能为主要传播途径。

OBJECTIVE To statistically analyze the drug resistance of the Acinetobacter baumannii strains isolated from the submitted specimens that were obtained from patients in the ICUs or the environment and observe the ho‐mology so as to provide guidance for clinical treatment and control of nosocomial infections and cut the transmis‐sion route of the A .baumanni infection .METHODS The bacterial culture and identification were performed for the A .baumanni strains that were isolated from the ICU patients and environment by using Siemens MicroScan micro‐bial identification system ,the drug susceptibility was analyzed with the use of drug susceptibility system ;the ho‐mological analysis was performed by means of the Enterobacterial Repetitive Intergenic Consensus‐Polymerase Chain Reaction (ERIC‐PCR) and agarose gel electrophoresis .RESULTS Of 13 strains of A .baumannii ,10 strains were multidrug‐resistant ,of which 7 strains were resistant to the commonly used antibiotics ,incl

目的比较社区感染耐甲氧西林金黄色葡萄球菌(MRSA)与医院感染MRSA的耐药率,了解其耐药趋势,为临床对MRSA的防治提供科学依据。方法筛选中山医科大学第一附属医院2009~2012年从各临床标本中分离出的不重复MRSA菌株,采用MicroScan PC 12或PC 20鉴定药敏复合板进行鉴定和药敏实验,参照中华人民共和国卫生部医政司医院感染监控小组制定的诊断标准,将其分为社区感染MRSA与医院感染MRSA两组,比较分析两组的耐药率。结果社区感染MRSA与医院感染MRSA的感染部位显著不同,痰、伤口分泌物是其主要标本来源。医院感染MRSA多见于痰和伤口分泌物,痰标本由2009年的30例上升到2012年的103例,伤口分泌物标本由2009年的4例上升到2012年的28例,增幅较为显著;医院感染MRSA的耐药性普遍高于社区感染MRSA,其中对环丙沙星、克林霉素、利福平的耐药性显著高于社区感染MRSA。结论医院感染MRSA的耐药性高于社区感染MRSA,社区感染MRSA的耐药率有逐年上升的趋势。

Objective To compare of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) and hospital resistance rate of MRSA infection, to understand the trend of drug resistance for clinical prevention and treatment of MRSA to provide a scientiifc basis for. Method Screening hospital from 2009 to 2012 from various clinical specimens isolated from the non-repetition MRSA strains identiifed by MicroScan PC 12 or PC 20 composite plate identiifcation and drug susceptibility susceptibility test, in the light of the PRC Ministry of Health, Medical Administration hospital infection control team to develop diagnostic criteria, will be divided into community-acquired MRSA and hospital infection MRSA group, compared resistance rates were analyzed. Results Community-acquired MRSA and hospital infection MRSA is obviously different sites of infection, sputum, wound secretion is the main source of specimen;both drug resistance there are some differences. Conclusion Resistance of MRSA hospital