目的:采用TK基因突变试验评价土荆芥精油的遗传毒性,为其安全性评价提供资料。方法:土荆芥精油浓度设置分别为体积分数0.002%、0.004%、0.006%和0.008%,对L5178Y细胞进行3 h染毒处理;同时分别设二甲基亚砜(DMSO)和环磷酰胺(CP)为溶剂对照和阳性对照,微孔板法进行TK基因突变试验。检测土荆芥精油对L5178Y细胞的细胞毒性及TK基因位点突变频率。结果:随土荆芥精油浓度的增大,L5178Y细胞相对存活率(RS)、相对悬浮生长率(RSG)和相对总生长率(RTG)较溶剂对照组有下降趋势;0.008%剂量组时,RS、RSG和RTG分别为溶剂对照组的29.38%、38.13%和5.75%,表现出明显的细胞毒性;与溶剂对照组相比,突变频率(TMF)随土荆芥精油浓度增加则呈上升趋势(F=410.5,P=0.00)。TMF在土荆芥精油各浓度组间以及和阳性、溶剂对照组间的多重比较差异均有统计学意义(P〈0.05)。小集落百分比(SC)均超过95%。结论:土荆芥精油具有一定的细胞毒性,可诱导L5178Y细胞TK基因位点突变频率增高,有一定的致突变性。
OBJECTIVE:The genotoxicity of essential oil fromChenopodium ambrosioides L. was evaluated withTK gene mutation test to provide information for its safety. METHODS:The mouse lymphoma cells L5178Y were exposed to essential oil ofC. ambrosioides at volume fraction concentrations of 0.002%, 0.004%,0.006% and 0.008% for 3 h. 1% dimethyl sulfoxide (DMSO) and 10μg/mL cyclophosphamide (CP) were used as solvent and positive controls,respectively.TK gene mutation was assessed with microwell method to detect cytotoxicity and mutation frequency atTK locus in L5178Y cells.RESULTS:Relative survival (RS),relative suspension growth (RSG) and relative total growth (RTG) decreased when concentration of essential oil fromC. ambrosioides increased,with only 29.38%,38.13% and 5.75%,respectively,in 0.008% dose group indicating significant cytotoxicity. Compared with the solvent control mutation frequency (TMF) increased with the rising concentration of essential oil(F=410.5,P=0.00). Multiple comp