目的：检测肺癌外周血中细胞角蛋白19（CK19）mRNA、细胞角蛋白20（CK20）mRNA和基质金属蛋白酶-9（MMP-9）mRNA，探讨其作为判断晚期肺癌外周血检测其转移及判断预后的指标可行性。方法采用RT-PCR技术检测肺癌患者外周血中CK19mRNA、CK20mRNA、MMP-9mRNA的表达量，按表达阴阳性作为标准对其临床资料及短期疗效进行评估。结果 CK19、MMP-9及CK20表达阳性率化疗3疗程后均下降，其中CK19、MMP-9差异有统计学意义（P值0.003、0.007）；CK19mRNA、CK20mRNA和MMP-9mRNA表达阳性率化疗前后与性别，分化等均无相关性（P＆gt;0.05）；其中化疗前CK19mRNA表达Ⅳ期阳性率高于Ⅲ期患者（P=0.01）；腺癌患者CK19mRNA与MMP-9mRNA表达，化疗前表达阳性率明显高于鳞癌差异有统计学意义（P值为0.01、0.04）；化疗后腺癌CK19mRNA、CK20mRNA、MMP-9mRNA表达较鳞癌表达下降明显（P值为0.04、0.03、0.02）；治疗前CK20mRNA和MMP-9mRNA表达与有无疗效存在相关性，差异有统计学意义（P值为0.04、0.0.02），CK19mRNA表达无相关性。化疗后CK19mRNA、CK20mRNA表达与有无疗效存在相关性，差异有统计学意义（P值为0.01、0.02），MMP-9mRNA表达无相关性。结论通过CK19、CK20、MMMP-9mRNA表达检测，

Objective To investigate the feasibility that CK19,CK20 and MMP-9mRNA are considered as the index to detect the metastasis of peripheral blood of patients with intermediate and advanced-stage lung cancer and judge the prognosis. Method The expression amount of CK19mRNA,CK20mRNA and MMP-9mRNA in peripheral blood of patients with lung cancer was measured by RT-PCR and clinical data and the short-term curative effect were evaluated according to the standard of positive and negative expression. Results Positive expression rates of CK19,MMP-9 and CK20 were decreased after 3 course of chemotherapy,of which,the differences of CK19 and MMP-9 had statistical significance(P=0.003 and P=0.007,respectively);the positive expression rate of CK19mRNA,CK20mRNA and MMP-9mRNA before and after chemotherapy was not correlative with gender and differentiation etc(P>0.05);the positive expression rate of CK19mRNA of patients in stage IV was higher than that of patients in stage III before chemotherapy(P=0.01

目的观察雄性大鼠缺氧后头颈部真皮雌激素受体mRNA表达的变化。方法成年雄性Wistar大鼠置于缺氧舱中，每天缺氧处理4 h ，分别缺氧处理1、5、10、15、20、30 d。采用逆转录-聚合酶链式反应（RT-PCR），检测面部真皮雌激素受体（ER）α、βmRNA表达。结果缺氧处理后，ERβmRNA的表达则表现为缺氧后1天略有升高，但差异无统计学意义，5 d后ERβmRNA表达明显升高（P＜0．05），10 d后 ERβmRNA表达有所回落；15、20 d后ERβmRNA表达再次升高，其中20 d的表达与对照组比较差异有统计学意义（ P＜0．01）。缺氧30 d 后ERβmRNA表达再次下降至对照组水平。结论缺氧可在转录水平上影响大鼠面部真皮ERβmRNA表达。

Objective To observe the expression of estrogen receptor (ER) mRNA in male rat facial skin ex-posed to hypoxia .Methods Adult male Wistar rats were exposed to hypoxia module for 4 h everyday for 1 ,5 ,10 , 15 ,20 and 30 days .Levels of ERαand ERβmRNA in facial skin tissue were measured by using semi-quantitative re-al-time polymerase chain reaction .Results The expression of ERβmRNA increased since the 1st day ,but was not significant .While ,after the 5th day ,the expression level significantly increased (P<0 .05) .Level of ERβmRNA began to decrease after 10 days ,but increased again after 15 and 20 days of treatment ,and was significantly higher than control group after 20 days (P<0 .01) .After treatment of 30 days ,level of ERβmRNA were decreased again . Conclusion Hypoxia might affect the transcription of ERβin male adult rat facial skin .

目的：探讨IE mRNA和pp67 mRNA是否可以作为早期检测诊断人巨细胞病毒（HCMV）感染的标志物。方法采用荧光定量PCR的方法，在HCMV感染初诊患儿中检测IE mRNA和pp67 mRNA的表达特征。结果传统病毒DNA检测方法检测的阴性患儿中，仍然有一部分已经表达IE mRNA，而在初诊病毒DNA检测阳性的患儿中，100%表达HCMV感染后期的pp67 mRNA。结论 mRNA检测具有比DNA更高的灵敏性，应该根据不同时期病毒mRNA表达特征制定不同的治疗方案。

Objective Human cytomegalovirus (HCMV) can induce infection in multiple organs, including genitouri-nary system, central nervous system and liver. It can even lead to serious defects and death. China, as a high-incidence area of HCMV, need employ early treatment. However, the sensitivity of present HCMV diagnosis is not satisfied. Methods In the present study, we focused on transcription level of HCMV IE and pp67 mRNA using real-time quantitative PCR. Results IE mRNA was positive even in a portion of patients who were regarded as negative by traditional DNA diagnosis method. On the other hand, pp67 mRNA that were normally expressed at the late stage of HCMV infection was 100%posi-tive in the first-visit patients who were regarded as positive using traditional DNA diagnosis method. Conclusion The pres-ent study indicated that mRNA detection based on PCR method was more sensitive compared with DNA detection for assess-ing HCMV virus infection. Finally, different treatment should be perfo

目的研究绝经后骨质疏松症肾阴虚证差异表达基因CLCF1、ASB1和PROK2的mRNA表达水平。方法随机选择绝经后骨质疏松症患者,中医辨证肾阴虚证组30例,27例健康绝经后妇女为对照组,RT-PCR法检测外周血CLCF1、ASB1和PROK2 mRNA的表达。结果肾阴虚组CLCF1 mRNA表达水平明显低于对照组(Z=-2.621,P=0.009);肾阴虚组ASB1mRNA表达与对照组比较,有降低趋势,但差异无统计学意义;与健康对照组比较,肾阴虚组PROK mRNA表达差异无统计学意义。结论骨质疏松症肾阴虚证与CLCF1 mRNA表达下调关联。

Objective To investigate the mRNA expression of CLCF1, ASB1, and PROK2 in postmenopausal osteoporosis patients with the kidney Yin deficiency.Methods Patients with postmenopausal osteoporosis were randomly selected.Thirty patients were selected as the kidney Yin deficiency syndrome group, and another 27 patients with normal bone mineral density ( BMD) were selected as control group.The mRNA expression of CLCF1, ASB1, and PROK2 in the peripheral blood was detected using real-time RT-PCR.Results The expression of CLCF1 mRNA in the kidney Yin deficiency group was significantly lower than that in the control group (Z=-2.621, P=0.009).Compared with that in the control group, the expression of ASB1 mRNA in the kidney Yin deficiency group showed a declining tendency, but no significant difference was observed.Compared with that in control group, no significant difference of PROK mRNA expression in the kidney Yin deficiency group was observed.Conclusion Down-regulation of CLCF1 mRNA expression

探讨TCTE3 mRNA在人肝癌细胞株中的表达水平及意义。采用实时荧光定量PCR技术分别检测TCTE3基因在常规培养或缺氧诱导（CoCl2为诱导剂）的人肝癌细胞株中的表达情况。TCTE3 mRNA在常规培养的人正常肝细胞株L02，肝癌细胞株Bel-7402、SMMC-7721、HepG2和QGY-7701中不表达或极低表达；但经缺氧诱导后，SMMC-7721细胞中的TCTE3 mRNA表达量逐渐升高，4h达到最大，随后逐渐降低，与HIF1a mRNA表达的曲线变化有一定相似之处，只是这种变化的时间要早于HIF1a基因。在缺氧状态下肝癌细胞TCTE3 mRNA表达增加，且这种上调表达可能对HIF1a mRNA的表达有一定的促进作用。

To investigate genes expression and significance of TCTE3 mRNA in liver cancer cells cultured. TCTE3 mRNA were examined in liver cancer cells under conventional culture or hypoxia-induced culture by CoCl2 by real-time quantitative Polymerase chain reaction (PCR). No expression or low expression of TCTE3 mRNA levels in normal human liver L02 cells, and liver cancer Bel-7402, SMMC-7721, HepG2 or QGY-7701 cells. However, After SMMC-7721 cells were incubated with hypoxia-inducible CoCl2, the expression of TCTE3 mRNA began to increase gradually, peaked at 4h, and then decreased, which has some similarities with HIF1a mRNA expression curve, but such changes earlier than HIF1a mRNA. The expression of TCTE3 mRNA levels increased, which could promote HIF1a mRNA expression.