目的：研究Vimentin在宫颈癌组织中的表达与淋巴转移及宫旁浸润的关系，以探讨Vimentin预测宫颈癌淋巴转移及宫旁浸润的可能性．方法免疫组化法检测宫颈癌组织微阵列芯片78例组织标本Vimentin表达，数据用SPSS软件进行统计分析处理，＜0.05为差异有统计学意义．结果（1）78例宫颈癌组织中Vimentin阳性表达率为30/78（38.5%），Vimentin在宫颈癌组织中表达与淋巴转移和宫旁浸润相关，淋巴转移组Vimentin表达率为15/31（48.4%），显著高于淋巴转移阴性组的15/47（31.9%）；（2）宫旁浸润组Vimentin表达率为17/32（53.1%），显著高于宫旁无浸润组15/46（32.6%）的表达率（＜0.05）．结论 Vimentin在宫颈癌组织中的表达与淋巴转移及宫旁浸润之间存在相关性，检测宫颈癌组织Vimentin可有效预测宫颈癌淋巴转移及宫旁浸润．

Objective To investigate the relationship between the Vimentin expression and lymph node metastasis of cervical cancer in order to explore the possibility of its predictive value on the lymph node metastasis and parametrial infiltration. Methods The expression of Vimentin in cervical cancer tissue microarray chip which contains 78 cases was evaluated by immunohistochemistry method to study the relationship between the expression and lymph node metastasis and parametrial infiltration of cervical cancer. Results The expression of vimentin in 78 cases of cervical cancer tissue was 30/78 (38.5%) .The expression of vimentin in cervical cancer was significantly related to lymph node metastasis and parametrial infiltration respectively. The expression of Vimentin in lymph node metastasis positive group was 15/31 (48.4%), which were significantly higher than that in lymph node metastasis negative group. The expression of Vimentin in parametrial infiltration positive group was 17/32 (53.1%), wh

目的 探讨波形蛋白(Vimentin)、E-钙黏蛋白(E-cadherin)在原发性肝癌(HCC)的表达及其临床意义.方法 采用免疫组织化学的方法(SP法)分别检测55例HCC癌组织、癌旁组织和20例非肿瘤性正常肝组织中Vimentin及E-cadherin的表达情况,分析它们与HCC临床病理学特征的关系及对患者预后的影响.结果 Vimentin在癌组织中阳性表达率为69.1％,较癌旁组织及正常肝组织表达明显升高,差异具有统计学意义(P＜0.05).E-cadherin在癌组织中阳性表达率为27.3％,较癌旁组织及正常肝组织表达明显下降,差异具有统计学意义(P＜0.05).Vimentin、E-cadherin二者表达呈负相关关系(r=-0.653,P＜0.05).Vimentin在TNMⅢ～Ⅳ期组、低分化组、肿瘤结节多个组、转移复发组的表达分别高于TNM Ⅰ～Ⅱ期组(x2=7.267,P＜0.05)、中高分化组(x2=4.045,P＜0.05)、肿瘤结节单个组(x2=12.143,P＜0.05)、无转移复发组(x2=7.267,P＜0.05).E-cadherin的表达在肿瘤结节单个组高于肿瘤结节多个组,差异具有统计学意义(x2=6.878,P＜0.05).随访患者表明,Vimenfin阳性表达组患者无病生存率明显低于Vimentin阴性表达组,差异具有统计学意义(P＜0.05).结论 Vimentin、E-cadherin参与HCC上皮间质转化过程,Vimentin的高表达、E-cadherin的低表达与HCC的发生、发展密切相关.检测HCC患者Vimentin及E-cadherin的表达水平可协助HCC的诊断及判断预后.

Objective To investigate the molecular expression and clinical significance of Vimentin and E-cadherin during epithelial-mesenchymal transformation of hepatocellular carcinoma (HCC) tissues.Methods Hepatocellular carcinoma and paracancerous tissue were collected from 55 patients,while nonneoplastic nonnal liver tissue were collected from 20 patients without cancerous diseases as control group.Expressions of Vimentin and E-cadherin were examined by immunohistochemistry staining.Clinicopathological features of the relative expression of Vimentin and E-cadherin,and its influence on prognosis were discussed as well.Results Vimentin was positively expressed in 69.1％ cancer tissue,and was significantly higher than paracancerous tissues and normal liver tissues(P ＜ 0.05).E-cadherin was positively expressed in 27.3％ cancer tissue and was significantly lower compared with paracancerous tissues and normal liver tissues(P ＜0.05).The expression of Vimentin and E-cadherin was negative

目的探讨N钙黏蛋白(neuronal cadherin,N-cadherin)和波形蛋白(vimentin)在原发性直肠腺癌中的表达及其临床意义。方法选择手术切除并经病理证实的58例原发性直肠腺癌组织和距肿瘤5cm以上的正常直肠黏膜组织,采用免疫组织化学SP法检测组织中N-cadherin和vimentin的表达并与临床资料进行相关性分析。结果直肠腺癌组织中N-cadherin和vimentin阳性表达率[53.4%(31/58)、46.6%(27/58)]明显高于正常直肠黏膜组织[3.4%(2/58)、1.7%(1/58)](P0.01);N-cadherin和vimentin的阳性率与肿瘤浸润深度、淋巴结转移、远处转移和TNM分期呈正相关(P0.05),与肿瘤分化程度无相关性(P0.05)。结论 N-cadherin和vimentin的表达与原发性直肠腺癌的转移密切相关,检测N-cadherin和vimentin的表达可作为判断直肠腺癌转移和预后的指标之一。

Objective To observe the expression of neuronal cadherin (N-cadherin) and vimentin in primary rectum adenocarci-noma tissues and their clinical value .Methods 58 cases of primary rectum adenocarcinoma tissues after surgical resection and con-firmed by pathology ,and normal rectum tissues that at lest 5 cm away from the tumor were selected .The immunohistochemical SP method was used to detect the expression of N-cadherin and vimentin and analyzed their correlation .Results In rectum adenocarci-noma tissues ,the positive expression rate of N-cadherin and vimentin[53 .4% (31/58) ,46 .6% (27/58)]were significantly higher than those of in normal rectum mucosa tissues[3 .4% (2/58) ,1 .7% (1/58)] ,(P 0 .05) .Conclusion N-cadherin and vim-entin have close relationships with metastasis of rectum adenocarcinoma ,to detect the expressions of N-cadherin and vimentin is of a effective method to judge the metastasis and prognosis of rectum adenocarcinoma .

目的检测Snail、E-cadherin及Vimentin在上皮性卵巢癌组织中的表达,探讨Snail介导的上皮间质转化(EMT)在卵巢癌发生、发展及转移中的作用。方法采用免疫组化法分别检测Snail、E-cadherin、Vimentin在48例卵巢浆液性腺癌、卵巢交界性浆液性肿瘤及卵巢浆液性腺瘤中的表达,探讨EMT相关因子表达强度的相关性及与临床病理特征之间的关系。结果 (1)Snail、Vimentin在卵巢浆液性腺癌中的表达率为(68.75%/66.67%),高于卵巢交界性浆液性腺瘤的(41.67%/37.50%)及卵巢浆液性腺瘤的(25.00%/18.75%),结果均具有显著的统计学差异(P0.05),E-cadherin在卵巢浆液性腺癌中的表达率为27.08%,低于与卵巢交界性浆液性腺瘤的54.17%及卵巢浆液性腺瘤的75.00%,结果均具有统计学差异(P0.05)。而卵巢交界性浆液性腺瘤组与卵巢浆液性腺瘤组比较,三种蛋白的表达均无统计学差异(P0.05)。(2)Snail、E-cadherin及Vimentin的表达高低与FIGO分期、分化级别、有无淋巴结转移及腹膜种植有关。(3)在卵巢浆液性腺癌中,Snail与Vimentin的表达呈正相关(r=0.477,P0.05),Snail与E-cadherin的表达呈负相关(r=-0.601,P0.05),而E-cadherin与Vimentin表达的相关性不明显(r=-0.206,P0.05)。结论在上皮性卵巢癌组织中,Snail、Vimentin表达上调,E-cadherin表达下调,提示Snail介导的EMT可能与卵巢癌的发生、发展及浸润转移有关。

Objective To Study the effect on Snail mediated epithelial mesenchymal transition (EMT) in ovarian cancer occurrence, development and metastasis. Methods Immunohistochemical staining was detected Snail, E-cadherin,in 48 cases of ovarian serous adenocarcinoma,ovarian serous borderline tumors and ovarian serous adenomas Vimentin,investigate the correlation between the expression of EMT -related factors and the strength and relationship between clinicopathological features. Results(1)The expression of Snail,Vimentin in ovarian serous adenocarcinoma was 68.75% and 66.67%,higher than in borderline serous ovarian tumors (41.67% and 37.50) and in ovarian serous adenomas( 25.00%and 18.75%),with significant difference(P 0.05).(2)The expression level of Snail and FIGO, E-cadherin and Vimentin staging,differentiation level,there was no lymph node metastasis and peritoneal implants.(3)in ovarian serous adenocarcinoma, the expression of Snail positively correlated with Vimentin (r=0.477,P < 0.05),t

【目的】探讨肺腺癌组织中上皮‐间叶转换（EMT）相关蛋白的表达及意义。【方法】采用免疫组化法检测100例肺腺癌及40例癌旁正常肺组织中的细胞角蛋白（CK）、波形蛋白（Vimentin）、α‐平滑肌肌动蛋白（α‐SMA）的表达，并分析其临床意义。【结果】肺腺癌组织中CK、Vimentin、α‐SMA阳性表达率分别为51．0％、60．0％、59．0％，与癌旁正常组织中各蛋白的表达率（分别为72．5％、25．0％、27．5％）比较均有显著性差异（P＜0．05），CK、Vimentin、α‐SMA与分化程度相关，与年龄、性别、浸润深度、临床分期及远处转移不相关。【结论】CK与a‐SMA、Vimentin呈现负相关，分化程度降低，CK含量降低，α‐SMA、Vimentin呈现逐渐上升的趋势，提示肺癌恶变程度与EMT有关。

[Objective]To explore the expression and significance of epithelial mesenchymal transition (EMT) related proteins in lung adenocarcinoma tissues .[Methods]Immunohistochemical method was used to detect the epxression of CK protein ,vimentin protein and a‐smooth muscle actin protein(α‐SM) in 100 cases of lung adenocarcinoma and 40 cases of normal lung tissue adjacent to carcinoma .Clinical significance was ana‐lyzed .[Results] The positive rates of CK ,vimentin protein and α‐SM protein in lung adenocarcinoma tissues were 51 .0% ,60 .0% and 59 .0% respectively ,and those in normal tissues adjacent to carcinoma were 72 .5% , 25 .0% and 27 .5% respectively ,and there were signficant differences( P<0 .05) .The expressions of CK ,vi‐mentin protein and α‐SM protein were related to tumor differentiation ,but not related to age ,sex ,invasive depth ,TNM stages and distant metastasis .[Conclusion]CK is negatively correlated to α‐SM and vimentin . When the differentiation degr