Trigram sequence of the“Zhou Yi”sixty-four trigrams is inconclusive so far. This study uses magic square theory, and combined with the Fu Xi sixty-four trigrams square diagram, constructs the sixty-four trigrams magic map, and the universe symmetry principle model. The basic structure and principle of this model is described and explained, and the arrangement and distribution of one Yin and one Yang trigrams, two Yin and two Yang trigrams, three Yin and three Yang trigrams, six Yin and six Yang trigrams, twelve Pi trigrams and sixteen Mi-zi trigrams are analyzed, and reached the research conclusion of “magic principle decided the ancient trigram sequence”. At last, by using the conclusions of this research, the sixty-four trigrams horse step magic figure as verification is constructed.