The softwood cuttings propagation experiment using the half-lignified twigs of 4-year-old Tectona grandis L.f.was per-formed in the last ten-days of May,June,July,August and September.The variation of the soluble sugar ,total nitrogen,ratio of C/N, soluble protein,activity of peroxidase,etc,were studied in the same time,in order to discuss the impact of physiological and biochem-ical indices on rooting of cuttings propagation.The results showed that ,in different sprouting period ,differences of peroxidase ,soluble sugar,total nitrogen,ratio of C/N,rootage ratio are significant at 1%,differences of soluble protein are not significant;Positive rela-tivity between ratio of C/N and rootage ratio is quite high.The best time of softwood cuttings propagation for Tectona grandis L.f.in 4 years old is in July to August.