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双语推荐：柚木无性系

对从老挝、中国海南、缅甸选出的柚木优树进行组培，并培育出23个无性系苗木，以中国海南柚木种子园1个家系的实生苗作为对照，开展柚木的山地造林试验，在造林30个月后对幼林的树高、胸径生长量、保存率、枯梢率进行了测定与分析。研究结果表明：不同的柚木无性系在高、径生长上均存在着极显著差异，表明开展柚木优良无性系选择十分必要；综合柚木的生长量及适应性看，表现较好的无性系为2号、24号、22号；入选的无性系高、径生长量及适应性均优于种子园的实生苗，可作为当地造林的繁殖材料。

With the tissue culture from Laos, Hainan Province of China, Burma elected superior tree of Tectona grandis, we conducted the mountain afforestation experiment by using cultivation 23 clonal seedlings and seedling of one family in Tec-tona grandis orchard of Hainan (as control), and analyzed their tree height, DBH increment, preservation rate and dry treetop rate after 30-month plantation.Different clones in height and diameter growth are significant differences, indicating that the selection is necessary about excellent clones of Tectona grandis.By the integrated views in growth and adaptability of clones, No.2, 24 and 22 are with good performance.Selected clones in high, diameter growth and adaptability are bet-ter than seedling in orchard, and can be used as local propagation material in afforestation.

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以柚木优良无性系71-14组培苗节间茎段为材料,MS为基本培养基,采用正交设计对6-BA、IBA、TDZ、NAA等4个生长调节剂各4水平进行愈伤组织诱导,并以最佳组合使用不同浓度的TDZ进行柚木愈伤组织再生。结果表明:TDZ对形成具再生能力的致密型愈伤组织影响最大,低浓度水平的TDZ和6-BA更易形成致密型愈伤组织;以愈伤组织大小、诱导率和致密型所占比例采用隶属函数法评定得出最优的愈伤组织诱导培养基为MS+0.9 mg·L-16-BA+0.04 mg·L-1IBA+0.02 mg·L-1TDZ+0.8 mg·L-1NAA,愈伤组织诱导率达80.78%、平均直径1.65 cm,获致密型愈伤组织83.0%;得出优化的再生培养基为MS+0.132 mg·L-1TDZ,分化率为34.22%;初步建立了以茎段为外植体的柚木优良无性系71-14的再生体系,为柚木转基因技术的研究提供技术支撑。

To establish a teak (Tectona grandis )regeneration system for further teak transformation,internode seg-ments for clone 71-14 were selected for callus induction and differentiation.In this study,6-BA、IBA、TDZ、NAA were used to study the effects of Plant Growth Regulators (PGRs)and their ratios on callus induction by an orthogonal de-sign with four concentrations,then the optimal callus were used for differentiation with different concentration of TDZ.The results showed that TDZ had the greatest influence on the types of callus,compact callus were much easier to be formed in low concentrations of TDZ and 6-BA.Meanwhile,using membership function which the evaluation in-dicators were callus size,callus induction rate and the compact callus rate,the optimal combination of PGRs for callus induction was 0.9 mg·L-1 6-BA+0.04 mg·L-1 IBA+ 0.02 mg·L-1 TDZ+0.8 mg·L-1 NAA,with 80.78% callus percentage,1.65 cm average diameter and 83.0 % compact callus percentage.The highest callus d