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双语推荐:根毛

根毛是根系特异化表皮细胞外伸形成的管状凸起物,是植物吸收矿质养分和水分的重要器官。根毛的发育可分为根毛细胞命运决定、根毛起始、根毛顶端生长和根毛成熟等阶段。本文对根毛发育生长过程中的细胞形态及其生理生化变化进行了综述,并从根表皮细胞命运决定分子机理, EXPANSIN、bHLH和MYB等转录因子以及小G蛋白和生长素/乙烯等方面简要说明了根毛生长发育的遗传基础。
Root hairs are long tubular outgrowths from specialized epidermal cells of roots. Root hairs can in-crease the root surface area for aiding plants in nutrient and water acquisition. Development of root hairs in-cludes four phases:root hair cell fate speciifcation, root hair initiation, root hair tip growth and root hair matu-ration. This review represents cells morphology and the physiochemical changes in four phases of root hairs, followed by discussion about the molecular mechanisms of root hair cell fate specification, the regulatory mechanisms of TFs (EXPANSIN, bHLH and MYB) ROPs and auxin/ethylene on root hairs development.

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目的单根毛囊单位在眉毛种植中的应用和心得体会方法选用耳后较细的毛发采用FUE方法提取单根毛囊,在显微镜下经专业毛囊分离人员加工分离成单根毛囊,将分离好的单根毛囊单位种植于眉毛缺失处。结果应用FUE方法提取单根毛囊,眉毛种植60例,术后随访6~12个月,眉毛生长良好,效果满意,无并发症。有5例患者自觉密度欠佳,再次加密。结论单根毛囊单位进行眉毛种植,效果自然,无并发症,术后眉毛更浓密、更自然。
Objective TO application and experience of single hair follicle unit in planting the eyebrow Methods Use FUE method to extract single hair follicle of fine hair at rear of the ear. After separated by professional under the microscope, the single follicle units will be planted at the hair missing area in the eyebrow. Results Apply FUE method to extract single hair follicle, eyebrow plantation 60 cases and followed up satisfactory, well growing of eyebrow, no complications in the 1-12 months after the operation.Conclusion The single follicle unit of eyebrow planting is proved with natural effect without complications and the eyebrow looks bushy and natural after the operation.

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田间栽培的柑橘根毛少甚至无,高度依赖丛枝菌根替代根毛吸收养分,因而柑橘丛枝菌根的研究被广泛关注。本文综述了近20年国内外柑橘丛枝菌根的形态特征、根际丛枝菌根真菌的资源、丛枝菌根的生理和分子研究以及菌根功能的影响因子的最新进展,重点通过植株生长、营养吸收和抗逆性阐明丛枝菌根的生理功能。
Citrus plants possess less or no root hairs in the fields and thus highly depend on arbuscular mycorrhizas to replace the root hair for nutrient uptake from soil .Therefore,arbuscular mycorrhizas in citrus have been widely concerned .This paper summarizes the new advances in the research of morphology ,fungal re-sources in rhizosphere ,physiology and molecular studies of arbuscular my corrhizas in citrus ,and factors impac-ting mycorrhizal functionings .It clarifies the physiological functions of arbuscular mycorrhizas in terms of plant growth ,nutrient uptake and adverse tolerance .

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以莴苣幼苗为材料,检测了分离自细果角茴香的生物碱对莴苣幼苗根生长及根毛发育的影响。结果表明:50~200μmol·L-1浓度范围内,8-甲氧基二氢血根碱对莴苣幼苗根长显著抑制;10、20和30μmol·L-18-甲氧基二氢血根碱对莴苣幼苗根毛的长度和数量有显著的抑制作用,且抑制作用均表现了浓度依赖性。通过对莴苣根尖细胞有丝分裂研究发现,50μmol·L-1的8-甲氧基二氢血根碱能显著抑制根尖细胞的有丝分裂,而且莴苣幼苗净增值率与根尖细胞的有丝分裂之间呈正相关,证明8-甲氧基二氢血根碱主要通过抑制根尖细胞有丝分裂对莴苣根的生长产生影响。
Effects of 8-methoxy dihydrosanguinarine isolated from Hypecoum leptocapum on the root growth and root hair development of Lactuca sativa seedlings were studied.The results showed that the root growth was signifi-cantly inhibited by 8-methoxy dihydrosanguinarine at the concentration of 50-200μmol??L-1;the inhibitory effect on root hairs increased with concentration ranging from 10 to 30μmol??L-1 .The inhibitory effects of tested compound on the length and number of root hairs showed a dose-dependent manner.Moreover,the mitosis of apical cells could be inhibited by 8-methoxy dihydrosanguinarine at the concentrations above 50μmol??L-1 and revealed a positive linear regression with root NGR.Thus,the effect of 8-methoxy dihydrosanguinarine on Lactuca sativa seedlings mainly ex-plored on the inhibition of the mitotic activity.

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应用双进样口、双毛细管色谱柱以及质谱检测器(MSD)和氢火焰检测器(FID),通过四通分流装置在一台带FID的气相色谱质谱联用仪(GCMS)上面实现一根毛细管柱同时采集MSD和FID双检测器信号数据;同时安装两根不同极性的柱子,不需要拆装柱子,进行无痕切换使用来满足不同样品分析要求。
This paper introduces the mathod of MSD and FID simultaneous detection in 6890N/5975C gas chromatograph-mass spectrometer and the mathod can meet different analysis without column ex-change .

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植物应对磷胁迫的方法之一是改变根系的构型.以水稻 SUMO 化 E3连接酶 SIZ1突变体 ossiz1为供试材料,研究了 OsSIZ1在水稻根发育中的作用以及其与磷胁迫、生长素之间的关系.与野生型相比,OsSIZ1抑制 ossiz1种子根和不定根的伸长,促进侧根密度的增加和根毛的增多.缺磷时,突变体 ossiz1的反应更强烈,即不定根伸长、侧根密度增大和根毛增多的趋势更加明显.说明 OsSIZ1参与调控水稻根构型的改变,低磷时效果更明显.ossiz1地上部和地下部的总磷浓度显著高于野生型,说明 OsSIZ1在水稻中负调控磷素的吸收利用.定量 RT-PCR 结果显示,ossiz1中 OsYUCCA1和 OsP IN1a/1b的相对表达量显著高于野生型,说明 OsSIZ1负调控根中生长素的合成与极性运输,并且缺磷时负调控作用减弱.结果表明,SUMO 化 E3连接酶 OsSIZ1调控缺磷条件下根构型的形成,而且这一过程可能是通过调控生长素分布完成的.
Plants cope with the low P environment by altering their root architecture.Here,we studied the role of SUMO E3 ligase SIZ1 on the root development and architecture,and the relationship among OsSIZ1 ,phosphate stress and auxin using ossiz1 mutants.Compared with wild-type (WT),the ossiz1 mutation inhibited the elongation of seminal roots and adventitious roots,promoted lateral roots formation and root hair proliferation.However,the response of the ossiz1 mutation was stronger than WT under Pi deficiency,namely,the trend of root elongation and the increase of the lateral root density and the root hair number of ossiz1 were more obvious relative to WT seedlings. This suggests that OsSIZ1 regulates the root architecture,and the effect is more obvious under Pi deficiency.The total P concentration of shoots and roots in ossiz1 is higher than WT,this suggests that OsSIZ1 negatively regulates phosphorus acquisition and use.And relative expression of OsYUCCA1 and OsP I N1a/1b in ossiz1

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为进一步明确油菜根肿病发病条件,通过室内盆栽试验研究pH值、土壤含水量、接种体浓度、温度等因素与油菜根肿病发生的关系。结果表明:pH值在4-7均可发病,其中pH5发病率及病情指数最高;肿根腐烂处理病情指数比未腐烂处理高;土壤含水量在60%左右发病最严重;接种体浓度越高发病越严重,低于104个休眠孢子/mL不发病;施氮肥会加重发病;光照周期和温度能够影响根毛侵染的情况。
To further clarify the occurrence conditions for rape clubroot disease ,some factors like pH ,soil moisture,inoculum concentration ,temperature,etc,were studied by means of indoor pot experiment .The results indicated that when soil pH was from 4 to 7 rape plants would contract the disease;the disease incidence and disease index were the highest when pH was 5;the disease index of club rot treatment was higher than that without the treatment;the disease was serious when the soil moisture content was about 60%;the higher the in-oculum concentration ,the higher the disease incidence and disease index , the host did not have the disease when the inoculum concentration was less than 104 resting spores/mL;applying nitrogen fertilizer could aggra-vate the disease;the infection in the root hairs was affected by photoperiod and temperature .

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混凝土材料所具有的多孔隙结构是由其本身的物理、化学性质所决定的,而孔隙结构又决定了混凝土整体的强度和渗透性。将混凝土复杂的孔隙结构简化为单根毛细管,根据 Fick 定律和质量守恒原理推导出混凝土孔隙溶解控制方程,并运用 Bear 理论中关于多孔介质毛细孔渗透率的研究理论及饱和溶质运移扩散方程建立侵蚀模型。模拟结果表明,该模型能够有效地反映混凝土孔隙中固相钙离子溶解、浓度变化和孔隙边壁侵蚀厚度变化过程。
The concrete material has a porous structure due to its own physical and chem ical properties, and the pore structure determ ines the strength and permeability of concrete. In this paper, the complex porous structure of concrete was simplified to the single capillary. According to the Fick''s law and mass conservation principle, the dissolution equation of concrete pores was obtained, and the erosion model was established based on the research of capillary permeability of porous medium in Bear theory and saturated solute t ransport diffusion equations. The simulation results showed that the model can reflect the variations of dis-solution and concent ration of solid phase calcium ions and erosion thickness of side wall inpores.

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拟南芥氨基酸透性酶1( AAP1,也叫NAT1)是植物中第一个被分离的氨基酸转运蛋白,在拟南芥主根、侧根、根毛及根表皮细胞中均有增加氨基酸含量的作用。本研究利用RT-PCR的方法,从拟南芥中克隆了该基因,测序结果与 GenBank(NM-104616?4)中已发表的拟南芥 AAP1(AtAAP1)基因的同源性高达99?93%;并将AtAAP1基因与pCAMBIA3300构建p3300?AAP1植物表达载体,为过表达AtAAP1基因,提高作物氨基酸含量,从而改善作物氮素利用效率提供参考。
Arabidopsis amino acid permease 1 ( AAP1, also called NAT1) is the first separated a?mino acid transporter in plants, and AAP1 gene is an important amino acid transporter to increase the content of amino acids in main roots, lateral roots, root hairs and root epidermal cells. In this study, AtAAP1 gene was cloned from Arabidopsis thaliana by RT-PCR. Sequence homology analysis showed that it had high similarity (99?93%) with Arabidopsis thaliana AAP1 that has been submit?ted to GenBank ( NM-104616?4). The plant expression vector p3300?AAP1 was constructed by gene AtAAP1 and pCAMBIA3300. It is the over expression of AtAAP1, which can increase amino acid content of crops, and thereby provide reference for improving nitrogen use efficiency.

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以模式植物拟南芥为研究材料,探讨了Ca在拟南芥根生长发育及抵御盐胁迫过程中的重要生理作用。结果表明,低Ca条件会导致拟南芥幼苗鲜质量降低、抑制主根生长、侧根发育及根毛发育与生长。研究发现,50 mmol/L NaCl胁迫会导致对照培养植物产生1个细胞质Ca2+([Ca2+]cyt)升高峰值,为0.325μmol/L;而低Ca培养植物产生3个[Ca2+]cyt升高值,升高频率明显增多,最高达0.467μmol/L,是对照的1.40倍。由此推断,低Ca条件使植物对盐胁迫更敏感。
We choose Arabidopsis as material. The research studies on the important physiology role of calcium in root development and resistance to salt stress. In this study,the fresh weight,primary root,lateral roots and root hairs of Arabidopsis thaliana were all inhibited on the calcium deprivation media. The plants grown on control media were evoked a transient rise of cytosolic Ca2+( [ Ca2+] cyt ) by 50 mmol/L NaCl,while the plants grown on calcium deprivation media were evoked three. The peaks of signal were 0. 325,0. 476 μmol/L,respectively. It is suggested the plants were more sensitive to salt stress under the calcium deprivation condition.

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