利用SSR分子标记技术对热辣3号辣椒杂交种进行纯度鉴定。从85对SSR引物中筛选出10对引物在热辣3号亲本间表现明显的多态性,均为共显性标记。为了提高鉴定结果的准确性,选用位于辣椒不同染色体上的3对共显性标记对热辣3号辣椒进行纯度检测,种子纯度为99.49%。分子标记鉴定与表型鉴定比较分析表明,2种鉴定结果高度一致。研究结果表明,SSR分子标记可以用于热辣3号辣椒杂交种纯度的快速、准确检测。

Establishing a rapid and stable seed purity identification technology system is the effective measure for controlling the pepper hybrid quality. The development of molecular marker techniques could provide an accurate and fast way for identifying the crops hybrid purity. In this study,SSR markers were applied to testing the purity of Rela No. 3. 10 pairs among 85 pairs of SSR primers showed significant polymorphisms between the parents of Rela No. 3. All of them were co-dominant markers. In order to improve the accuracy of identification results,three co-dominant SSR markers located on different chromosomes were selected for purity test of Rela No. 3. The seed purity was 99 . 49%. Comparative analysis showed that the identification results of molecular markers and phenotypes were highly consistent. The study results indicated that SSR markers could be used for hybrid purity test of Rela No. 3, quickly and accurately.

为了建立杂交黄瓜品种川翠3号的快速纯度鉴定体系，用164对全基因组覆盖的SSR引物对川翠3号及其亲本进行了多态性筛选及纯度鉴定。其中4对引物表现出稳定的共显性，电泳图谱清晰、易分辨，特异性强，对川翠3号种子鉴定纯度为99.0豫，真、假杂种编号与田间鉴定结果一致，可以用来区别其中混杂的母本、父本及其他种子，说明这4对引物组成的指纹图谱能为川翠3号杂交种的真伪鉴定、纯度检测及亲本提纯等提供准确的技术指导。

In order to establish fast purity identification system for hybrid cucumber cultivar Chuancui No.3 seed, we used 164 pairs SSR primers which covered whole genome for purity identification and polymorphic analysis of cucumber cultivar Chuancui No.3 and its parents. The results showed that, four primers were stably codominant, their electrophoretogram were clear, easy to distinguish, and had high specialty, its molecular marker identification purity was 99.0% by detecting some cucumber cultivar Chuancui No.3 seeds, which was the same as the field identification result,they could be used to distinguish medley female parent, male parent and other seeds. So, the fingerprint with these four pairs primers could provide more accurate technical guidance for authenticity identification, purity test and parents purification of hybrids.

杂交种纯度优劣直接决定了杂交种产量，而常规方法鉴定周期长，不能及时反映杂交种纯度，难以指导生产应用。由于种子混杂和SSR位点杂合都会造成株间带型的差异，为提高杂交种纯度鉴定的准确性，本研究从196对SSR引物中筛选到4对在双亲间表现稳定多态性的引物，根据带型特点进行组合，结果显示，利用多重PCR-SSR技术可以区分棉花杂交种种子混杂和SSR位点杂合，为杂交种快速准确鉴定提供技术支持。

Hybrid seed purity decides the hybrid yield. The conventional identification method lasts long time and can not display the seed purity promptly, so not to meet the production need. Since it is difficult to distinguish the bands pattern differences of hybid lines caused by mixed seeds and SSR loci heterozygous, in order to improve the accuracy of hybrid purity identification, 4 pairs of SSR primers showing stable polymorphism in the parents were selected from 196 pairs of primers, and combined according to the characteristic of PCR banding pattern, the results showed that the multiplex PCR-SSR technology could distinguish hybrid seed impurity and SSR loci heterozygous, so as to provide technical support for rapid and accurate seed purity identification of hybrids.

SSR标记具有快速、稳定、准确的技术优势，已经在品种鉴定等方面得到广泛的应用。为了鉴定生产用“两杂”种子样品的遗传多样性及纯度，利用36对SSR引物对39份杂交水稻样品进行了遗传多样性鉴定，其中2个样品为“同名异种”（遗传距离为0.11），有2个样品疑似“异名同种”；利用33对SSR引物对36份杂交玉米样品进行了遗传多样性鉴定，同样存在“同名异种”和“异名同种”现象。在人为混杂的杂交水稻样本中，SSR标记检测值与实际混杂率u测验没有显著性差异；同时利用SSR标记和田间鉴定方法比较种子公司提供的10个杂交水稻样品纯度，尽管有3个样品SSR标记检测结果低于田间鉴定，但由于田间纯度鉴定以农艺性状为标准，遗传背景相似的单株难以区分，因此从加强种子质量监管角度考虑，SSR分子标记鉴定技术更为有利。

SSR molecular markers which had the technical advantages of fast, stable and accurate were used to investigate the genetic diversity and purity test of two hybrid seed commercially available. 36 rice SSR primer pairs were used to assess the genetic diversity of 38 hybrid rice samples. The result showed two of the sample were heterogenous (genetic distance 0.11), whereas two of the others seemed to be synonym (genetic distance 0.03). 33 maize SSR primer pairs were used to determine the genetic diversity of 36 hybrid maize sample, the result showed two of the sample were heterogenous (genetic distance 0.12). In the artificial mixed sample test, u test revealed there was no significant difference between all sample test value and actual mixed ratio. Meanwhile, 10 hybrid rice samples provided by seed company were compared with SSR molecular marker and field identification methods, though SSR marker test results were below the field identification in threesamples, the field identifying were

基于分子标记的茄子（Solanummelongena）杂交种子纯度快速检测技术体系的建立对茄子杂交品种的研究具有重要的意义。本研究以茄子杂交品种“苏崎3号”、“苏崎4号”及其双亲为试验材料，采用NaOH裂解法快速提取DNA与SSR标记分析相结合的方法，对茄子杂交品种种子纯度的鉴定技术进行了研究。结果表明，利用NaOH裂解法提取的DNA能够进行正常的SSR分析。从46对SSR引物中各筛选出1个共显性标记，对‘苏崎3号”和“苏崎4号”种子进行群体检测，发现分子标记鉴定结果与田间鉴定结果一致。研究结果表明NaOH裂解法和SSR标记分析相结合可实现对茄子杂交品种种子纯度的快速和准确鉴定。

It is important to construct a molecular marker based detection system of seed purity for eggplant hybrid. In this paper, two eggplant hybrids,‘Suqi 3’and‘Suqi 4’, and their parents were used as plant materials. Rapid DNA extraction by NaOH method and SSR analysis were used to construct a system for the identification of seed purity of eggplant hybrids. The data showed that the DNA quality was suitable for SSR amplification. By screening 46 pairs of SSR primers, two co-dominance SSR markers were identified for‘Suqi 3’and‘Suqi 4’ respectively. Further seed purity identification of two hybrid populations by SSR markers showed the coincident results of field identification. Above results indicated that the combination of DNA extraction by NaOH method and SSR analysis is a rapid and accurate method for the detection of eggplant hybrid seed purity.