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双语推荐：2表达

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目的探讨胃癌组织中血小板反应蛋白2(THBS2)基因表达水平微血管密度(MVD)、基质金属蛋白酶(MMP)-2表达的相关性。方法应用免疫组化EnVision二步法染色检测MMP-2表达水平。应用CD34免疫组化染色评价MVD的表达。通过PCR技术定量分析80例胃癌组织和30例正常胃黏膜组织中THBS2基因的表达,分析胃癌组织中THBS2基因的表达与MVD、MMP-2表达的相关性。结果胃癌组织中THBS2基因表达为(-1.2±0.05),正常的胃黏膜组中为(-1.82±0.12),两组之间的差异显著(P0.05)。THBS2基因高表达与低表达与MMP-2蛋白表达具有密切的关系(P0.05);胃癌组织中MVD 45.34±12.35,与THBS2基因水平呈正相关(r=0.432,P=0.001)。结论胃癌组织中THBS2基因的表达水平于MVD、MMP-2表达存在一定的相关关系,THBS2基因的变化可能是通过促进MVD、MMP-2表达实现的。
Objective To study correlation between the microvascular density ( MVD) and matrix metalloproteinase ( MMP)-2 ex-pression of THBS2 gene in gastric cancer tissue .Methods Immunohistochemical EnVision two steps staining was used to detect MMP-2 ex-pression level, the immunohistochemical staining of CD34 was applied to evaluate the MVD expression ,expression of THBS2 gene in 80 cases of gastric cancer tissue and 30 cases of normal gastric mucosa tissues were analysed by quantitative PCR technique .Relationship between MMP-2,MVD expression and the expression of THBS 2 gene in gastric carcinoma was analysed .Results The average value of the expression of THBS2 gene in gastric cancer tissue was (-1.2 ±0.05),while the average THBS2 gene expression in normal gastric mucosa was (-1.82± 0.12) (P<0.05).There was a close relationship between the high ,low expression of THBS2 gene and MMP-2 protein expression (P<0.05).The average expression of THBS2 gene in gastric carcinoma tissue was

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目的探讨恒温快速冷冻切片对乳腺癌组织中Cerb B-2蛋白表达的影响。方法应用Max Vinsion免疫组织化学技术,对130例浸润性导管癌患者的恒温冷冻切片对应组织和剩余组织石蜡标本进行标记,比较两组Cerb B-2蛋白表达情况。结果 130例浸润性导管癌冰冻切片对应组织样本组中42例Cerb B-2表达阴性,33例Cerb B-2表达+,34例Cerb B-2表达++,21例Cerb B-2表达+++;剩余组织常规切片样本组56例Cerb B-2表达阴性,50例Cerb B-2表达+,19例Cerb B-2表达++,5例Cerb B-2表达+++;两组标记结果比较具有非常显著性差异,而且两组Cerb B-2阳性表达结果一致性较差。结论恒温快速冷冻切片影响乳腺癌组织中Cerb B-2蛋白表达,减弱乳腺癌组织中Cerb B-2蛋白阳性表达。
Objective To explore the influence of frozen section on the expression of CerbB-2 in patients with advanced breast cancer .Methods The CerbB-2 expression of tissues after frozen section and the rest tissues in 130 cases of advanced breast cancer were detected by Max vinsion immunohistochemistry ,and the difference of the 2 groups were compared .Results There ware 42 cases of CerbB-2 negative expression ,33 cases of CerbB-2+expression ,34 cases of CerbB-2++expression and 21 cases of CerbB-2+++expression in the frozen section group .There were 56 cases of CerbB-2 negative expression ,50 cases of CerbB-2+expression,19 cases of CerbB-2++expression and 5 cases of CerbB-2+++expression in the rest tissues by routine section .The difference of the CerbB-2 expression of the 2 groups was significant , and the CerbB-2 expression of the 2 groups had low degree of consistency .Conclusion The frozen section affects the CerbB-2 expression in the advanced breast cancer,and decreases the Cerb

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目的 检测胰腺导管腺癌(PDAC)组织中Musashi 2(MSI2)蛋白和mRNA的表达,分析癌组织MSI2表达与临床病理参数的相关性.方法 应用免疫组化方法检测61例PDAC组织及配对癌旁组织MSI2蛋白表达水平,采用蛋白质印迹法及实时PCR法检测10例癌组织及配对癌旁组织MSI2蛋白及mRNA的表达.分析癌组织MSI2表达与肿瘤临床病理参数的关系.结果 61例PADC患者癌组织MSI2高表达率为63.9％,配对癌旁组织为41.0％,癌组织的表达显著高于配对的癌旁组织,差异有统计学意义(t =2.809,P=0.007).10例癌组织及癌旁组织的MSI2蛋白表达量分别为0.748±0.195、0.420±0.171;癌组织MSI2 mRNA的表达量为癌旁组织表达量的(2.507±2.981)倍,差异均有统计学意义(t=3.689,P=0.005;t=2.660,P=0.026).癌组织MSI2表达仅与肿瘤大小呈正相关(x2=5.096,P =0.024),而与其他参数均无相关性.MSI2高表达患者的术后中位生存期为321 d,低表达者为730 d,高表达者的生存期显著短于低表达者,差异有统计学意义(x2=6.706,P=0.010).结论 胰腺癌组织中MSI2表达上调,其表达量与肿瘤大小相关,高表达患者的预后差.
Objective To detect the expression of MSI2 protein and mRNA in pancreatic ductal adenocarcinoma (PDAC) tissue,and investigate the correlation between the expression of MSI2 protein and the clinicopathological parameters.Methods The expression of MSI2 protein in 61 PDAC specimens and paired adjacent non-cancerous pancreatic tissues were detected by immunohistochemistry.Western blot and quantitative real-time PCR (QRT-PCR) were used to examine the expression of MSI2 protein and mRNA level in 10 PDAC specimens and adjacent non-cancerous pancreatic tissues.Then the relationship between MSI2 expression in cancerous tissues and clinicopathological parameters was analyzed.Results In 61 patients with PDAC,the expression rate of MSI2 protein was higher in cancerous tissues (63.9％) compared with that in paired non-cancerous pancreatic tissues (41.0％),and the difference between the two groups was statistically significant (t =2.809,P =0.007).The expression levels of MSI2 protein in 10

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探讨小细胞肺癌c-myc癌基因的表达对Nrf2表达的影响,分析c-myc与Nrf2表达的相关性。方法:选用两株耐药性不同的肺小细胞癌肿瘤细胞株,小细胞肺癌药物敏感株H69及耐药株H69AR,Western-Blot方法比较Nrf2,c-myc在两株细胞间的表达差异;H69细胞通过Lipofectamine2000脂质体转染过表达c-myc,Western-Blot方法检测过表达c-myc前后Nrf2蛋白表达变化;H69AR细胞通过Lipofectamine2000脂质体转染c-myc siRNA,Western-Blot方法检测c-myc siRNA前后Nrf2的蛋白表达变化。结果:与H69细胞相比,H69AR细胞株中c-myc及Nrf2高表达;H69细胞c-myc转染后,Nrf2表达上调;H69AR细胞c-myc siRNA干扰后,Nrf2表达下调。结论:小细胞肺癌中Nrf2的表达与c-myc表达呈正相关。
Objective:To determine the influence of oncogene c-myc over Nrf2 in small cell lung cancer, and to an-alyze the correlation between c-myc and Nrf2 expressions. Methods:Two lines of small-cell lung cancer cells were chosen in this study. One is drug sensitive H69 cell. The other is multidrug resistant H69AR cell. Western blot assays were uti-lized to compare the different expression levels of c-myc and Nrf2. Overexpression of c-myc by Lipofectamine 2000 trans-fection was performed in H69 cells, and Nrf2 protein expression was compared between the control and c-myc transfection groups. Down-regulation of c-myc by siRNA transfection was performed in H69AR cells, and Nrf2 protein expression was compared between the control and c-myc siRNA groups. Results: Compared with H69 cells, protein expression level of c-myc and Nrf2 was much higher in H69AR cell. In H69 tumor cells, Nrf2 was up-regulated after c-myc transfection. Mean-while, in H69AR tumor cells, Nrf2 was down-regulated after c-myc s

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研究血管内皮生长因子(VEGF)、环氧化酶-2(COX-2)在91例HER-2阴性乳腺癌组织中的表达及相互关系,分析其与临床病理特征及预后的关系。方法:采用免疫组化SABC法,检测91例HER-2阴性乳腺癌组织中VEGF、COX-2蛋白的表达,并与临床病理特征进行相关性分析。结果:HER-2阴性乳腺癌组织中VEGF阳性表达率为94.5%,其中47.2%为高表达(+++),VEGF的表达与肿瘤大小、淋巴结转移相关(P0.05)。HER-2阴性乳腺癌组织中COX-2阳性表达率为86.8%,其中34.0%为高表达(+++),COX-2的表达与患者年龄、组织学分级、肿瘤大小、淋巴结转移及TNM分期无关(P0.05)。VEGF与COX-2的表达呈正相关(r=0.1798,P=0.018),VEGF/COX-2均高表达的患者淋巴结转移率高。结论:VEGF、COX-2在HER-2阴性乳腺癌组织中均高表达且两者呈正相关,联合检测VEGF、COX-2的表达对HER-2阴性乳腺癌预后判断可能具有重要价值。
Objective:To study the expression of vascular endothelial growth factor( VEGF)and cyclooxygenase-2(COX-2)in HER-2 negative breast cancer tissues of 91 patients and analyze the correlation with clinicopatho-logic features and prognosis. Methods:The expression of VEGF and COX-2 proteins in HER-2 negative breast cancer tissues of 91 patients were detected using immunohistochemistry SABC method. The relationship between COX-2 and VEGF expression was analyzed,together with clinicopathologic features. Results:The positive expression rate of VEGF was 94. 5% in the 91 HER-2 negative breast cancer tissues. The overexpression(score 3+)rate of VEGF was 47. 2%. The overexpression of VEGF was related with tumor size and lymphatic metastasis(P 0. 05). The expression of VEGF was related to the ex-pression of COX-2(r=0. 1798,P=0. 018). Overexpression VEGF/overexpression COX-2 tumors were proved to have high lymphatic metastasis. Conclusion:In HER-2 negative breast cancer tissues,VEGF and COX

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目的探讨乳腺癌患者人表皮生长因子受体-2(CerbB-2)表达与肿块大小及淋巴结转移的关系。方法采用免疫组织化学法(SP)检测80例乳腺癌患者CerbB-2表达情况,并分析其与肿块大小及淋巴结转移的关系。结果 68%(45例)乳腺癌患者CerbB-2阳性表达,CerbB-2在肿块2cm组,2~5cm组,5cm组阳性表达率分别为44%(8例)、59%(32例)、62%(5例)。CerbB-2阳性表达与肿块大小无关(P0.05)。49%的乳腺癌患者有淋巴结转移,CerbB-2在淋巴结转移者中的阳性表达率为77%,CerbB-2阳性表达与淋巴结转移有关(P0.05)。结论 CerbB-2阳性表达与肿块大小无关,CerbB-2阳性表达者易出现淋巴结转移。
Objective To explore the relationship between the expression of CerbB-2 and t tumor size , lymph node metastasis in breast cancer .Methods The expression of C-erbB2 in 80 cases with breast cancer was detected with immunohistochemical technique (SP) ,then the relationship between the expression of CerbB-2 and tumor size ,lymph node metastasis was analyzed .Results Positive expression of CerbB-2 was found in 68% cases of breast cancer .The positive rates of CerbBof CerbB-2 were 44% ,59% and 62% in three different tumor size groups ,respectively .There was no significant association between CerbB-2 expression and tumor size (P>0.05) . Lymph node metastasis was found in 49% cases of breast cancer .The positive rates of CerbB-2 were 77% in cases with lymph node metastasis .Expression of CerbB-2 was correlated with lymph node metastasis (P<0.05) .Con-clusion There was no significant association between the expression of CerbB-2 and tumor size .Lymph node me-tastasis is easy to develop in the

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目的 探讨Her-2基因表达与腋淋巴结转移数目之间关系及对预后的影响.方法 选择2008年1月至2011年1月收治的351例原发性乳腺癌患者为研究对象,采用免疫组织化学法检测Her-2基因表达,分析其与腋淋巴结转移数目之间关系及对预后的影响.结果 Her-2基因表达-、+、++与腋淋巴结转移数目之间无相关性(x2=3.757,1.650,2.379,P＞0.05);Her-2基因表达+++与腋淋巴结转移数目之间存在相关性(x2=8.681,P＜0.05);Her-2基因表达-、+、++、+++的2年生存率分别为77.01％(201/261),85.00％(34/40),29.17％(7/24),11.54％(3/26),其中Her-2基因表达-～+的2年生存率明显高于Her-2基因表达++～+++者,差异有统计学意义(x2=61.605,P＜0.01);腋淋巴结转移数目为0枚和1～3枚时,Her-2基因表达-～+的2年生存率明显高于Her-2基因表达++～+++者,差异有统计学意义(x2=61.605,14.747,P＜0.05).腋淋巴结转移数目为4～9枚和≥10枚时,Her-2基因表达-～+的2年生存率与Her-2基因表达++～+++者比较差异无统计学意义(x2=3.691,3.482,P＞0.05).结论 Her-2基因表达-、+、++与腋淋巴结转移无相关性,Her-2基因表达-～+的2年生存率高于Her-2基因表达++～+++者,而Her-2基因表达-与Her-2基因表达+在判断预后上无区别;
Objective To investigate the relationship between expression of Her-2 gene and the transfer number of axillary lymph node and its influence on prognosis.Methods A total of 351 cases with primary breast cancer from January 2008 to January 2011 were selected.The expression of Her-2 gene was detected by immunohistochemical method,and analyzed the relationship between it and the transfer number of axillary lymph node and its influence on prognosis.Results The expression of Her-2-,+,++ had no correlation with the transfer number of axillary lymph node (x2 =3.757,1.650,2.379,P ＞ 0.05),while the expression of Her-2 +++ had correlation with the transfer number of axillary lymph node (x2 =8.681,P ＜ 0.05).The 2 years survival rates in the expression of Her-2-,+,++,+++ were 77.01％ (201/261),85.00％ (34/40),29.17％(7/24),1 1.54％ (3/26),and which in the expression of Her-2--+ was significantly higher than that in the expression of Her-2 ++-+++ (x2 =61.605,P ＜ 0.01).The transfer numbe

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目的：分析乳腺癌组织中Pirh2和Naked2的表达情况及其临床意义。方法：随机选取在2011年1月—2013年12月期间收治的96例乳腺癌标本作为观察组，同时选取癌旁组织作为对照组，应用免疫组织化学方法对Pirh2和Naked2表达情况进行检测，分析Pirh2和Naked2表达与乳腺癌病理分级的关系及Pirh2和Naked2两者的相关性。结果：Pirh2在乳腺癌组织中高表达，癌旁组织中低表达，Naked2在乳腺癌组织中低表达，癌旁组织中高表达(P＜0.05)；年龄、肿瘤大小与癌中Pirh2和Naked2的表达无关(P>0.05)；肿瘤病理分级和淋巴结转移与Pirh2和Naked2的表达相关(P＜0.05)；Pirh2和Naked2的表达呈负相关。结论：Pirh2和Naked2对乳腺癌的生物学行为的判断及预后有一定的提示作用。
Objective:To analyze the expression and clinical significance of Pirh2 and Naked2 in breast cancer. Methods:98 breast cancer specimens from Jan.2011 to Dec.2013 were randomly selected as the observation group , while the adjacent tis-sues were selected as a control group. The immunohistochemistry method was used to detect the expression of Pirh2 and Naked2 in 96 breast cancer cases, analyze the pathology character, and then detect the relationship between them. Results:Pirh2 was highly expressed in breast cancer, lowly expressed in adjacent tissues. However Naked2 was lowly expressed in breast cancer tissues, highly expressed in adjacent tissues, the difference was significant(P 0 . 05 ) . Pirh2 and Naked2 were correlated with tumor grade and lymph node metastasis(P<0.05). The expression of Pirh2 and Naked2 in breast cancer tissue was negative(P<0.05). Conclusion:Pirh2 and Naked2 has an important role in the breast cancer and has suggestive action in biological behavior and p

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为了克隆猪输血传播病毒2型（TTV2）ORF1基因，构建原核表达载体，在大肠杆菌BL21（DE3）中诱导表达猪TTV2 ORF1蛋白，并对其表达条件进行优化。利用普通PCR从猪TTV2阳性样本中扩增出TTV2 ORF1基因，利用分子克隆的方法将猪TTV2 ORF1基因克隆到原核表达载体pcoldI上，构建表达载体pcold-ORF1，在大肠杆菌中诱导表达带有6个组氨酸标签的ORF1融合蛋白。并对影响重组蛋白表达的3个因素，即诱导时间、诱导温度和IPTG浓度进行优化，确定了pcold-ORF1重组蛋白表达的最佳表达条件。 SDS-PAGE分析结果表明，重组蛋白在BL21中高效表达，以包涵体形式表达为主，分子质量约为39 kDa。蛋白表达量随诱导时间增加而有所增加，在15℃时表达量最高，而IPTG浓度对蛋白的表达量没有显著影响。 Western Blotting 结果表明，重组蛋白与猪TTV2阳性血清特异性反应，具有很好的免疫原性。成功获得TTV2-ORF1基因，构建了原核表达载体并获得高效表达，为TTV的ELISA检测方法的建立提供抗原。且重组蛋白在15℃条件下，加入0．2 mmol/L浓度的IPTG，诱导24 h，表达条件最佳。
To clone the open reading frame 1(ORF1)gene of porcine torque teno virus type 2 and construct a prokaryotic expression vector .TTV2 ORF1 recombinant protein was expressed in Escherichia coli and optimal ex-pression was performed.The ORF1 gene of TTV2 was amplified from the TTV2 positive samples and sub-cloned in a prokaryotic expression vector pcoldI .The constructed recombinant plasmid pcold-ORF1 was transformed to E.coli BL21 for expression the ORF1 fusion protein with six histidine-tag under induction of IPTG .The induction time ,in-duction in different temperature and IPTG concentrations were optimized .The recombinant fusion protein had high expression level in BL21(DE3),and SDS-PAGE analysis showed the exogenous gene was mainly expressed as in-clusion bodies and its molecular weight was about 39 kDa.The induction time were positively related trends with re-combinant protein expression ,the best induction temperature was 15 ℃,whereas IPTG concentration had no signifi-To clone the

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目的：探讨尾侧型同源转录因子2（caudal type homeobox transcription factor-2,CDX2）和黏蛋白2（mucin 2, MUC2）的表达与萎缩性胃炎和胃癌的关系。方法采用组织芯片技术构建含30例正常胃黏膜、30例萎缩性胃炎和41例胃癌的组织芯片,用免疫组化方法（S-P 法）检测组织芯片中 CDX2和 MUC2的表达。结果正常胃黏膜组织中CDX2、MUC2都无表达。萎缩性胃炎中 CDX2（100%）、MUC2（72.40%）高表达。胃癌黏膜 CDX2（66.67%）、MUC2（13.89%）阳性表达。CDX2、MUC2在不同胃黏膜阳性表达率差异有统计学意义（P〈0.001）。CDX2和 MUC2在胃癌组织中的表达与病人年龄、浸润深度、有无淋巴结转移、TNM 分期均无关（P〉0.05）。CDX2表达在男女性别差异有统计学意义（P〈0.05）。CDX2表达与组织学类型、分化程度都无关（P〉0.05）。MUC2表达在不同组织学类型、分化程度差异有统计学意义（P〈0.05）。CDX2、MUC2二者的表达无明显相关性。结论 CDX2、MUC2表达在胃黏膜肠化生进展及胃癌发生中有重要作用。
Objective To investigate the expression of caudal type homeobox transcription factor - 2 (CDX2)and mucin 2 (MUC2)in atrophic gastritis and gastric carcinoma,and their correlation with atrophic gastritis and gastric carcinoma. Methods Tissue chips containing 30 atrophic gastritis specimens,41 gastric carcinoma specimens,and 30 normal gastric mucosa specimens were constructed by tissue microarray technolo-gy,at the same time,the expressions of CDX2 and MUC2 in tissue microarrays were determined by using im-munohistochemistry. Results Neither CDX2 expression nor MUC2 expression occurred in normal gastric mucosa tissue specimens.High expression of CDX2 (100%)and MUC2 (72.40%)occurred in atrophic gastri-tis specimens.Gastric carcinoma mucosa had CDX2 -positive expression (66.67%)and MUC2 -positive ex-pression (13.89%).The comparison of CDX2- and MUC2- positive expression among different gastric mu-cosa specimens yielded statistical significant differences (P 0.05).Compared the mal

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